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Induction of Apoptosis in Human Osteosarcoma Cell Lines(Saos-2) by Single Fraction High Dose Irradiation (고용량 방사선 조사 후 골육종 세포주(Saos-2)의 아포프토시스 발생)

  • Kim, Jae-Do;Chung, So-Hak;Hong, Young-Gi;Choi, Jang-Seok
    • The Journal of the Korean bone and joint tumor society
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    • v.5 no.1
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    • pp.1-8
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    • 1999
  • A single fraction of 50 Gy extracorporeal irradiation, as a modality of limb-sparing operation, has been used to achieve tumor necrosis in osteosarcoma. Although this modality of radiation therapy preserving the mobility of a joint is commonly practiced, the precise knowledge on the radiobiological response of osteosarcoma cell has remained to be elucidated. We therefore observed whether a single high dose irradiation caused apoptosis in osteosarcoma cells and whether the commitment to apoptosis was associated with cell kinetics. We also investigated radiation dose response along the time course for development of apoptosis following single high dose irradiation. The morphologic change in apoptosis was observed by fluorescence with Hoechst 33258 and the degree and the fraction of cells by flow cytometry. Irradiation of osteosarcoma cells with 10, 30 and 50 Gy resulted in chromatin condensation and apoptotic body formation. The degree of apoptosis in osteosarcoma cells was $29.5{\pm}3.56%$, $39.9{\pm}4.83%$ at 24 and 48 hours after 10 Gy irradiation ; $41.1{\pm}3.93%$, $66.9{\pm}5.21%$ at 24 and 48 hours after 30 Gy irradiation ; and $48.0{\pm}3.69%$, $75.6{\pm}4.65%$ at 24 and 48 hours after 50 Gy irradiation. The fraction of cells in cell-cycle kinetic was $39.2{\pm}4.3%$ in G2/M, $22.1{\pm}4.65%$ in G1 at 24 hours after 10 Gy irradiation ; $51.0{\pm}4.3%$ in G2/M, $20.4{\pm}4.7%$ in G1 at 48 hours after 10 Gy irradiation ; $40.3{\pm}3.9%$ in G2/M, $26.1{\pm}4.7%$ in G1 at 24 hours after 30 Gy irradiation ; $59.2{\pm}3.9%$ in G2/M, $5.9{\pm}5.1%$ in G1 at 48 hours after 30 Gy irradiation ; and $44.3{\pm}4.2%$ in G2/M, $21.1{\pm}3.5%$ in G1 at 24 hours after 50 Gy irradiation. The fraction of cells at 48 hours after 50 Gy irradiation could not be observed because of irradiation induced cell death of most of cells. All values for irradiated cells showed accumulation in G2/M phase and reduction in G1 phase, irrespective of irradiation dose. The results suggest that a single fraction of high dose irradiation with 50 Gy results in accumulation of cells at G2/M phase, leading to apoptosis.

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A Study on Perception by Examines of the Radiology Department about Exposure to Radioactivity (영상의학과 내원 환자의 방사선피폭 인지도에 관한 연구)

  • Yeo, Jin Dong;Ko, In ho
    • Journal of the Korean Society of Radiology
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    • v.7 no.5
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    • pp.321-331
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    • 2013
  • The purpose of this study was to investigate factors of perception by inpatients of the radiology department about exposure to radioactivity at the time of radioactive examination, providing basic information for making educational materials aimed to change their perception. Most of those patients, 65.5% responded that CT was a type of radioactive examination bringing highest exposure to radiosensitivity. Most of the subjects, 56.1% said that a body part most vulnerable to exposure to radiosensitivity was the genital gland at the time of radiosensitivity examination. And most of the participants, or 26.3% responded that they obtained information about radiation from TVs or newspapers. Among the surveyed patients, men were higher in scores for factors of the perception of radiation such as recognition of radiation, harmfulness of radiation, psychological state at the time of radioactive examination, prevention of exposure to radioactivity and necessity of radiation that women were, with statistically significant differences between the two groups. These findings suggest that it is urgently needed to develop an education program which helps patients better perceive exposure to radioactivity and that radiologists should be very careful to reduce the does of that exposure. If patients better perceive radiation, they would be less anxious and less exposed to radioactivity when receiving the radioactive examination.

A Study on Perception by Examinees of the Dental Hospital Institutions about Exposure to Radioactivity (치과병원에 내원한 환자의 방사선피폭 인지도에 관한 연구)

  • Yeo, Jin-Dong;Ko, In-ho
    • Journal of the Korean Society of Radiology
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    • v.12 no.2
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    • pp.233-243
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    • 2018
  • The purpose of this study was to investigate factors of perception by inpatients of the Dental Hospital institutions about exposure to radioactivity at the time of radioactive examination, providing basic information for making educational materials aimed to change their perception. Most of those patients, 65.5.0% responded that CT was a type of radioactive examination bringing highest exposure to radiosensitivity. Most of the subjects, 56.1% said that a body part most vulnerable to exposure to radiosensitivity was the genital gland at the time of radiosensitivity examination. And most of the participants, or 26.3% responded that they obtained information about radiation from TVs or newspapers. Among the surveyed patients, men were higher in scores for factors of the perception of radiation such as recognition of radiation, harmfulness of radiation, psychological state at the time of radioactive examination, prevention of exposure to radioactivity and necessity of radiation that women were, with statistically significant differences between the two groups. These findings suggest that it is urgently needed to develop an education program which helps patients better perceive exposure to radioactivity and that radiologists should be very careful to reduce the does of that exposure. If patients better perceive radiation, they would be less anxious and less exposed to radioactivity when receiving the radioactive examination.

A Study on Perception by examinees of the Primary Health Care institutions about Exposure to Radioactivity (1차 의료기관에 내원한 환자의 방사선피폭 인지도에 관한 연구)

  • Yeo, Jin-Dong;Jeon, Byeong-Kyu
    • Journal of the Korean Society of Radiology
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    • v.9 no.6
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    • pp.381-392
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    • 2015
  • The purpose of this study was to investigate factors of perception by inpatients of the Primary Health Care institutions about exposure to radioactivity at the time of radioactive examination, providing basic information for making educational materials aimed to change their perception. Most of those patients, 65.5% responded that CT was a type of radioactive examination bringing highest exposure to radiosensitivity. Most of the subjects, 56.1% said that a body part most vulnerable to exposure to radiosensitivity was the genital gland at the time of radiosensitivity examination. And most of the participants, or 26.3% responded that they obtained information about radiation from TVs or newspapers. Among the surveyed patients, men were higher in scores for factors of the perception of radiation such as recognition of radiation, harmfulness of radiation, psychological state at the time of radioactive examination, prevention of exposure to radioactivity and necessity of radiation that women were, with statistically significant differences between the two groups. These findings suggest that it is urgently needed to develop an education program which helps patients better perceive exposure to radioactivity and that radiologists should be very careful to reduce the does of that exposure. If patients better perceive radiation, they would be less anxious and less exposed to radioactivity when receiving the radioactive examination.

Comparison of Yanluozi's and Huatuo's visceral figures (『연라자내경도(煙蘿子內境圖)』와 『화타선생내조도(華佗先生內照圖)』의 비교)

  • Koo, In-Mo;Kim, Hag-Dong;Kim, Ki-Wook;Lee, Byung-Wook
    • Journal of Korean Medical classics
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    • v.28 no.4
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    • pp.41-56
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    • 2015
  • Objectives : This research is aimed to compare the visceral figures published during the Song Dynasty, and to reveal the differences between anatomical and physiological knowledge in the presence of Taoist theoretical background and in its absence. It is also aimed to discuss the theoretical differences among existing medicines. Methods : First, we will find the commonalities and the differences in the drawings describing the internal organs of a human body in the two publications, and study what made such commonalities and differences and how they affected medical theories. Secondly, we will analyze and update the internal organ data specified in the two publications as it relates to the five viscera and six bowls. Finally, we will analyze the commonalities and the differences, and study the background and the significance of medical theories. Results & Conclusions : 1. The positions of the liver and the spleen in the drawing of the Yanluozi's and Huatuo's visceral figures are inverted in the drawing of the Huatuo's visceral figures. The positions of other internal organs are similar. 2. The section of the five viscera in the Yanluozi's and Huatuo's visceral figures describes the Taoist gods necessary to build up discipline by the means of meditation. 3. In the Zhutidian's visceral theory, a belly button is recognized as a very important organ for the activity of life. 4. In the Huatuo's visceral figures, the 'Zangzhen' generated from foods is described as the energy source for the vitality of the five viscera. 5. The Huatuo's visceral figures mentioned the functionality of a gall bladder and an Tripple Energizer, which was not mentioned in the Yanluozi's and Huatuo's visceral figures. 6. Both the "Yanluozi's and Huatuo's visceral figures" and the "Huatuo's visceral figures" specify that the moisture filtered through small intestines turns into urine.

Exercise Treatment of Knee Joint After Anterior Cruciate Ligament Reconstruction Operation (전방십자인대 재건술 후 슬관절 운동치료 접근에 관한 고찰)

  • Kim, Yeun-Jung;Jung, Jae-Min;Han, Jin-Tae
    • Journal of the Korean Society of Physical Medicine
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    • v.3 no.1
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    • pp.27-37
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    • 2008
  • Purpose : Today, it enjoys a sports and a leisure where the anterior cruciate ligament(ACL) injury patient increase. The knee joint is important means of human body movement and to do an important duty when it encounters ACL injuries of the knee joint and the many restriction follows in the life which is ordinary. When it is damaged ACL, it comes to determinate that ACL reconstruction and preservation treatment that the according to condition of ligament and knee joint. After ACL reconstruction, that is the fact which already becomes known the exercise treatment advances a recovery and to reduce a sequela. Methods : we researched the method of exercise treatment after anterior cruciate ligament reconstruction operation by journal of science direct and KISS in daecu university. Results : The representative exercise treatment is isometric exercise, isokinetic exercise isotonic exercise and complex exercise but what kind of exercise treatment most is effective, it revealed and support it was not. The method of exercise treatment is very various, so It causes a confusion made to the therapist and patients. So it executes once again it sought the kinetic therapeutic method which is efficient from this research and it tried to observe preceding research after ACL reconstruction. To operation a various the exercise treatments, operation only the treatment which is general compared to it was effective in muscular power and muscle functional improvement. But this like improve despite with the exercise treatment consequence which is continuous from research of most the pain leg compares to the health leg, it appeared the discrepancy which is a muscular power, a muscular endurance and a hypertrophy muscle etc, to the health leg or before operating 100% of muscular power to having gets the many therapy time was the recovery rate. Conclusion : Therefore after ACL reconstruction, it will become the many research continuously which is improve the muscle functional and ROM of the exercise treatment method and From therapeutic site of the patients it does to memorizes knowledge in advance about ACL injuries and the application the isokinetic treatment or exercise program are the set of necessary, frequency and amusement that considers complex what kind of therapeutic exercise becomes accomplished with the patient take care of attention.

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A Study on Urinary Incontinence, Interstitial Cystitis, Atrophic Viginitis of Elderly Women Using Senior Welfare Center and Nursing Home and the Cognition of Traditional Korean Medicine (복지관 및 요양원 이용 노인 여성의 요실금, 간질성 방광염, 위축성 질염 실태 및 한방치료에 대한 인식 조사)

  • Heo, Su-Jeong;Ie, Jae-Eun;Cho, Hyun-Ju;Myoung, Sung-Min;Sohn, Young-Joo
    • The Journal of Korean Obstetrics and Gynecology
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    • v.23 no.3
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    • pp.123-138
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    • 2010
  • Purpose: The purpose of this study is to identify the real condition of urinary incontinence(UI), interstitial cystitis(IC), atrophic viginitis(AV) for elderly women and analyze the cognition of traditional korean medicine(TKM) for them. Methods: We utilized questionnaire from May to June, 2010. Questionnaires were taken from 125 women using senior welfare center and nursing home, aged over 65 up to 92. The data were analyzed by $X^2$-test using SPSS/PC ver 18.0 program. Results: The prevalences of UI, IC and AV symptoms were 50.4%, 40.8%, 56%, respectively. The average I-QoL score for UI was $82.62{\pm}21.16$, and the average ICSI score for IC was $8.16{\pm}2.50$. After adjustment for each of the variables considered in this study, alcohol was associated with UI and age, BMI(body mass index) were associated with IC. Most of respondents have no experience(94.4%) or don't know (79.2%) about TKM for UI, IC and AV. 44 women(37.3%) indicated that they weren't willing to use TKM for UI, IC and AV. Reasons for not taking TKM were because of 'no knowledge of TKM(34.1%)' and 'more accustomed to western treatment(34.1%)'. Conclusion: Although the prevalence of UI, IC, AV for elderly women was high, the actual percentage of treatment for these diseases was low, furthermore, patients were not aware of TKM and had very few experiences of TKM for these diseases. The development and increased promotion about TKM program for elderly women's urogenital diseases is needed.

Parish Nursing : A New Challenge for Primary Health Care (지역교회간호(Parish Nursing) - 일차건강간호를 위한 새로운 도약)

  • No, Yu-Ja;Baek, Yeong-Mi
    • The Korean Nurse
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    • v.37 no.2
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    • pp.53-62
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    • 1998
  • ursing as a profession is characterized by its holistic, mind-body-spirit approach to the patient. Also, nurses have historically been the leaders in health education and promotion. Parish nursing has a great potential for providing primary preventive health care. services as well as assisting people to access the health care system. While working in the community, parish nurses see the church as the new arena for delivering health care services. The parish nurse program was introduced by Granger Westberg in 1984. The concept of parish nursing is based on several beliefs; health is multidimensional and affects all aspects of an individual-physical, psychological, social, and spiritaul being. Parish nursing is one model in which churches can cooperatively work with health care institutions to address the needs of their parishioners. The role of the parish nurse is emphasized in four basic area: a) health education, b) health counseling, c) referal services, and d) facilitation and organization of support groups within the congregation. The parish nurse programs work chiefly in congregation or commuity where a certain language of faith is ready at hand. This means that the parish nurse works in an ecology of meanings and care which encourages the drawing on the message of God's grace, the practices and habits it encourages. The parish nurse may be involved in the church's health ministries and may work on either paid or volunteer basis; however, one of the most important qualification of the parish nurse is to have the nursing knowledge and skills to practice within the standards of Nursing Practice Act. The completion of standards of practice for professional nurses practicing as parish nurses had been identified as a priority by the HMA Executive Board (1996, HMA). In conclusion, parish nursing promotes health and healing by empowering the faith community, family, or individual to incorporate health and healing practices. There are several preconditions that should proceed to establish the foundation for successful development of the parish nursing program in Korea. First, reciprocal relationship with home health nursing should be considered. Second, correct terms and concepts of parish nursing should be studied and understood. Third, systematic study and investigation should be followed for further development of parish nursing. Fourth, strengths and weaknesses of different models should be studied to develop proper model of parish nursing for Korean situation. Finally, consensus of standardized education program and corporation with various religious communities as well as health institutions should be established. When these preconditions are met, the role of parish nursing as a new program for the promotion of holistic health will be established.

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Consistency of 1-day and 3-day average dietary intake and the relationship of dietary intake with blood glucose, hbA1c, BMI, and lipids in patients with type 2 diabetes (제2형 당뇨병 환자의 1일과 3일 평균 식이섭취량의 일관성과 혈당, 당화혈색소, 체질량지수, 지질과의 관련성)

  • DaeEun, Lee;Haejung, Lee;Sangeun, Lee; MinJin, Lee;Ah Reum, Khang
    • Journal of Korean Biological Nursing Science
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    • v.25 no.1
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    • pp.20-31
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    • 2023
  • Purpose: This study aimed to determine the consistency of 1-day and 3-day average dietary intake using the 24-hour diet recall method and to investigate the relationship of diet intake with physiological indicators potentially associated with diabetic complications in patients with diabetes. Methods: This study conducted a secondary data analysis using pretest data of a nursing intervention study entitled "Development of deep learning based AI coaching program for diabetic patients with high risk and examination of its effects." Data were analyzed through descriptive analysis, one-way repeated-measures analysis of variance, and Pearson correlation coefficients using SPSS 26.0. Results: The average total daily calorie intake over 3 days was 1,494.48 ± 436.47 kcal/day: 1,510.90 ± 547.76 kcal/day on the first day, 1,414.22 ± 527.58 kcal/day on the second day, 1,558.34 ± 645.83 kcal/ day on the third day, showing significant differences (F = 3.59, p = .031). The correlation coefficient between the 1-day and 3-day average dietary intake was 0.41-0.77 for each nutrient and 0.62-0.80 for each food group. Vegetable intake showed negative correlations with body mass index (BMI; r = -.19, p = .023) and triglycerides (r = -.18, p = .036), whereas dairy intake was positively associated with low-density lipoprotein-cholesterol (LDL; r = -0.18, p = .034) and triglycerides (r = .40, p<.001). Conclusion: This study demonstrated that 1-day dietary intake was highly correlated with 3-day average dietary intake using the 24-hour diet recall method. Food groups showed significant associations with physiological indicators of potential diabetic complications such as BMI, triglycerides, and LDL levels. Further studies are needed to improve the knowledge base on the relationships between physiological indicators and food groups.

An Analysis of Image Use in Twitter Message (트위터 상의 이미지 이용에 관한 분석)

  • Chung, EunKyung;Yoon, JungWon
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.24 no.4
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    • pp.75-90
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    • 2013
  • Given the context that users are actively using social media with multimedia embedded information, the purpose of this study is to demonstrate how images are used within Twitter messages, especially in influential and favorited messages. In order to achieve the purpose of this study, the top 200 influential and favorited messages with images were selected out of 1,589 tweets related to "Boston bombing" in April 2013. The characteristics of the message, image use, and user are analyzed and compared. Two phases of the analysis were conducted on three data sets containing the top 200 influential messages, top 200 favorited messages, and general messages. In the first phase, coding schemes have been developed for conducting three categorical analyses: (1) categorization of tweets, (2) categorization of image use, and (3) categorization of users. The three data sets were then coded using the coding schemes. In the second phase, comparison analyses were conducted among influential, favorited, and general tweets in terms of tweet type, image use, and user. While messages expressing opinion were found to be most favorited, the messages that shared information were recognized as most influential to users. On the other hand, as only four image uses - information dissemination, illustration, emotive/persuasive, and information processing - were found in this data set, the primary image use is likely to be data-driven rather than object-driven. From the perspective of users, the user types such as government, celebrity, and photo-sharing sites were found to be favorited and influential. An improved understanding of how users' image needs, in the context of social media, contribute to the body of knowledge of image needs. This study will also provide valuable insight into practical designs and implications of image retrieval systems or services.