• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.648-657
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• 2008

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in $FB_1$ biosynthesis.

• Proceedings of the KIEE Conference
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• 2001.11a
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• pp.118-120
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• 2001

The $0.96MgTiO_3-0.04SrTiO_3+xLa(0{\sim}1.0wt%)$ ceramics were prepared by conventional mixed oxide method. The structural properties of $0.96MgTiO_3-0.04SrTiO_3+xLa(0{\sim}1.0wt%)$ ceramics with sintering temperature were investigated by the XRD and SEM. From the X-ray diffraction patterns, it was found that the perovskite $SrTiO_3$ and ilmenite $MgTiO_3$ structures were coexisted in the $0.96MgTiO_3-0.04SrTiO_3$ ceramics. The second phase of $Sr_{0.5}LaTi_2O_6$ was shown with addition of the $La_2O_3$. The dielectric constant(${\varepsilon}_r$), $Q{\times}f$ value and the temperature coefficient of the $0.96MgTiO_3-0.04SrTiO_3+0.2La$ ceramics were 21. 41, 39991, $-3.3ppm/^{\circ}C$, respectively.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.4
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• pp.502-509
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• 2013

It presents an efficient switching pattern, which expects a reduction of switching losses in a cascaded H-bridge PWM multilevel inverter. By the proposed switching scheme, the lower H-bridge module operates at low frequency of 60[Hz] because it assigns to transfer most load power. The upper H-bridge module operates at high frequency of PWM switching to improve THD of output voltage. The proposed switching pattern applies to cascaded H-bridge multilevel inverter with PD, APOD, bipolar, and unipolar switching methods. By computer-aided simulations, we verify the validity of the proposed switching scheme. Finally, we prove that the proposed PD and APOD switching patterns are better than those of the conventional one in efficiency.

• Journal of Ecology and Environment
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• v.38 no.4
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• pp.537-540
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• 2015

Social parasitism is occasionally found in some Polistes and Vespa species, such as Vespa dybowskii. We report a discovey of interspecific facultative social parasitism by Polistes sp. (possibly P. mandarinus) on Polistes djakonovi Kostylev in two rural areas of South Korea. P. djakonovi is very similar to Polistes sp. in its body color patterns except that the mark on the clypeus is different. In nest 1 (65 cells), we found 5 females of P. djakonovi and 4 females of Polistes sp. on 30 July 2014, whereas nest 2 (102 cells) contained 12 females and 16 males of P. djakonovi, and 3 females of Polistes sp. on 28 August 2013. Although we found the two nests in July and August, P. djakonovi seems to have been exploited by Polistes sp. at the end of the preemergence period (early to mid-June). The two nests found in this study had mainly white cocoon caps of P. djakonovi with several yellow ones of Polistes sp. In most cases of social parasitism, intruders have a larger size of the body or some body parts than the host in order to usurp the host; in contrast, this study showed that the hosts had lager bodies than the intruders.

• Smart Structures and Systems
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• v.18 no.6
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• pp.1203-1215
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• 2016

When strain sensing cables are under long-term stress and cyclic loading, creep may occur in the jacket material and each layer of the cable structure may slide relative to other layers, causing fatigue in the cables. This study proposes a device for testing the fatigue characteristics of three types of cables operating under different conditions to establish a decay model for observing the patterns of strain decay. The fatigue characteristics of cables encased in polyurethane (PU), GFRP-reinforced, and wire rope-reinforced jackets were compared. The findings are outlined as follows. The cable strain decayed exponentially, and the decay process involved quick decay, slow decay, and stabilization stages. Moreover, the strain decay increased with the initial strain and tensile frequency. The shorter the unstrained period was, the more similar the initial strain levels of the strain decay curves were to the stabilized strain levels of the first cyclic elongation. As the unstrained period increased, the initial strain levels of the strain decay curves approached those of the first cyclic elongation. The tested sensing cables differed in the amount and rate of strain decay. The wire rope-reinforced cable exhibited the smallest amount and rate of decay, whereas the GFRP-reinforced cable demonstrated the largest.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.1
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• pp.120-127
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• 2015

Morphological properties of dark banded rockfish (Sebastes inermis) were analysed to investigate its acoustic scattering characteristics. Total of 18 live samples was prepared for X-ray photos and collected morphological coordinates of their body and swim bladder shapes. Kirchhoff-ray mode model was used to calculate acoustic scattering pattern for broad-band frequency range. Inclination of swim bladder ranged from 17 to 30 and the averaged value was about $25.2^{\circ}$ (S.D.(standard deviation)=3.15). There were no any tendency of increase or decrease in volume and area ratio of swim bladder to fish body and ranged from 2.2 % to 4.43 % and 14.85 % to 21.31 %, respectively. The averaged value of volume and area ratio was 3.13 % (S.D.=0.52) and 17.6 % (S.D.=1.5). $b_{20}$ values were -69.01 for 38 kHz, -69.83 for 70 kHz, -70.17 for 120 kHz and -70.93 for 200 kHz, recpectively. Broadband acoustic patterns of dark banded rockfish for 20 ~ 200 kHz were similar among samples and they reflected size and morphological properties of fish species.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.3
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• pp.254-259
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• 2015

In this paper, The wireless robot control system was proposed using Brain-computer interface(BCI) systems based on the steady-state visual evoked potential(SSVEP). Cross Power Spectral Density(CPSD) was used for analysis of electroencephalogram(EEG) and extraction of feature data. And Linear Discriminant Analysis(LDA) and Support Vector Machine(SVM) was used for patterns classification. We obtained the average classification rates of about 70% of each subject. Robot control was implemented using the results of classification of EEG and commanded using bluetooth communication for robot moving.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.427-434
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• 2014

Although ex situ target strength (TS) measurements using dual- and split-beam systems have become the primary approach of estimating fish abundance, theoretical model estimation is a powerful tool for verifying the measurements, as well as for providing values when making direct measurements is difficult. TS values for 20 samples of live bambooleaf wrasse (Pseudolabrus japonicus) whose target length (TL) ranged between 13.7 and 21.3 cm were estimated theoretically using the Kirchhoff-ray mode model, and the TS values for 18 live fish samples were additionally measured at ${\sim}0^{\circ}$ tilt angle to the swimming aspect using a tethered method at a frequency of 120 kHz to verify the theoretical values. The digitizing intervals used to extract the fish body and swim bladder morphology in the X-ray photographs significantly affected the calculated TS patterns, but variations based on the speed of sound and density ratio values for the general range of fish flesh were relatively small (within 1 dB). Close agreement was observed between the measured and theoretical TS values, and the correlation between the average TS and body length of the fish could be calculated accurately as <$TS_{120kHz}$>= 20logTL (cm) -71.6 using the theoretical method.

• Journal of Nutrition and Health
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• v.16 no.2
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• pp.115-124
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• 1983

The biochemical nature of the protein constituents of six year old fresh Panax ginseng root was studied. Total protein constituents were extracted with phosphate buffer of pH 7.4, ionic strength of 0.1 and fractionated by ultrafiltration using four different membranes which cut down the materials of molecular weight of 500, 1,000, 5,000 and 10,000, respectively. Each fraction was subjected to ion exchange chromatography using DEAE - cellulose to isolate component proteins. The protein fraction larger than molecular weight of 10,000 was refractionated by the method of ammonium sulfate precipitation. The electrophoresis of the refractionated protein constituents was performed. The amino acid composition of the protein constituents was determined by gas- liquid chromatography. From the results, it could be summarized that eleven different protein constituents smaller than molecular weight of 10,000 were isolated from the fresh Panax ginseng root. At least eleven different protein constituents larger than molecular weight of 10,000 were identified from the electrophoretic patterns. These protein costituents seem to be compounded of all or some of five different subunits.

• Journal of Korean Public Health Nursing
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• v.30 no.1
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• pp.136-148
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• 2016

Purpose: Patterns of traditional medicine (TM) utilization vary depending on culture, historical significance, and regulations. We seek to understand how socio-demographic factors are related to TM use in Korea. Methods: The longitudinal cohort survey data of the Korea Health Panel (2008-2012) were used for the analysis. Information on TM included questions on 4 types of TM therapies used in South Korea (e.g., acupuncture, moxibustion and cupping, herbal therapies, and massage using hand). To adjust clustering across multiple observations per individual, individual random effects were used in the multivariate analysis. All analyses were performed using Stata 11.0. Results: A majority of Koreans (60%) used WM only during a year, and few people used TM only. Among TM users, most (93%) used it along with WM and 8% used TM only. Our regression analysis showed that female, age 34-64, low-educated, and sicker people were more likely to use TM. Conclusion: This study provides evidence on the relationship between TM utilization and socio-demographic factors.