• Title/Summary/Keyword: Blue Fluorescent

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Expression of the cyan fluorescent protein in fibroin H-chain of transgenic silkworm

  • Goo, Tae-Won;Choi, Kwang-Ho;Kim, Seong-Ryul;Park, Seung Won;Kim, Seong-Wan
    • International Journal of Industrial Entomology and Biomaterials
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    • v.34 no.1
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    • pp.11-15
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    • 2017
  • We constructed the fibroin H-chain expression system to produce enhanced cyan fluorescent proteins (ECFP) in transgenic silkworm cocoon. Fluorescent cocoon could be made by fusing ECFP cDNA to the heavy chain gene and injecting it into a silkworm. The ECFP fusion protein, each with N- and C-terminal sequences of the fibroin H-chain, was designed to be secreted into the lumen of the posterior silk glands. The expression of the ECFP/H-chain fusion gene was regulated by the fibroin H-chain promoter. The use of the 3xP3-driven EGFP cDNA as a marker allowed us to rapidly distinguish transgenic silkworms. The EGFP fluorescence became visible in the ocelli and in the central and peripheral nervous system on the seventh day of embryonic development. A mixture of the donor and helper vector was micro-injected into 1,020 Kumokjam, bivoltin silkworm eggs. We obtained 6 broods. The cocoon was displayed strong blue fluorescence, proving that the fusion protein was present in the cocoon. Accordingly, we suggest that the ECFP fluorescence silk will enable the production of novel biomaterial based on the transgenic silk.

The Effect of Fluorescent Whitening Agents on the Whiteness and the Shade of Fabrics in Repeated Washings (반복 세척시 형광증백제에 의한 증백효과와 색상변화)

  • 윤혜신;정혜원
    • Textile Coloration and Finishing
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    • v.12 no.3
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    • pp.192-198
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    • 2000
  • Influence of the fluorescent whitening agent(FWA)'s adsorption on the whiteness of cotton and on the color change of the dyed fabrics was investigated by repeating wash cycles. Cotton 100% and cotton60/polyester40 blended fabrics were dyed pink, blue and yellow, and cyanuric chloride diamino stilbene(CC/DAS) and distyryl bisphenyl(DSBP) were used for the FWA with laundry detergents. Fabrics were washed at $20^\circ{C}$ with Terg-o-tometer. The FWA adsorption amount was measured by the absorption intensity for the pyridine-water extracted solution. The FWA adsorption increased on the cotton fabric with the wash cycles. Though adsorption of CC/DAS continuously increased up to the 20th cycle, that of DSBP increased sharply before the 10th cycle and reached an equilibrium. The whiteness of the fabrics dried in the shade was greater than that dried under the sunlight through window glass. The color change of dyed fabrics was increased by the number of wash cycles. Pink changed more greatly than blue, yellow or grey cloth. The color change(\Delta{E)}$ of dyed fabric washed repeatedly up to the 20th cycle with the detergent without FWA was less than 1. It is recommended to wash pale colored fabric with the detergent free of FWA.

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Synthesis of titania nanopowder and its photocatalytic properties

  • Jang, Hee-Dong;Lee, Jae-Chun;Kil, Dae-Sup
    • 한국지구물리탐사학회:학술대회논문집
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    • 2003.11a
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    • pp.569-572
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    • 2003
  • Titanium dioxide ($TiO_2$) nanoparticles were prepared by the oxidation of titanium tetrachloride ($TiCl_4$) in a diffusion flame reactor. The average diameter of particles was 15 to 30 nm and mass fraction of anatase ranged from $40\;to\;80\%$. Effects of particle size and phase composition of those $TiO_2$ nanoparticles on photocatalytic properties such as decomposition of methylene blue and bacteria gas were investigated. The degree of decomposition of methylene blue by the $TiO_2$ nanoparticles under the illumination of the black light was directly proportional to the anantase mass fraction, but inversely to the particle size. The decomposition of bacteria by the $TiO_2$ nanoparticles under the illumination of the fluorescent light showed the same trend as in the case of the methylene blue.

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Variation of Plant Temperature at Joining Parts of Grafted Watermelon Seedlings Graft-taken under Different Light Quality (상이한 광질하에서 활착된 수박 접목묘 결합 부위의 식물체온 변화)

  • Kim, Y.H.;Lee, S.H.
    • Journal of Biosystems Engineering
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    • v.31 no.5 s.118
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    • pp.449-453
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    • 2006
  • Effect of light quality on plant temperatures at joining parts of grafted watermelon seedlings was investigated using a thermal imaging system in this study. Plant temperatures at joining parts lowered than those at stem region during graft-taking. However, difference in plant temperatures at joining parts and at stem region decreased by days after graft-taking. Plant temperatures of grafted seedlings graft-taken under white, blue, red, and blue + red fluorescent lamps repeatedly fluctuated high at photoperiod and low at dark period. Considering the variation of plant temperatures at joining pa퍼5, the illumination of blue and red light as well as white light will be effective for grafting of grafted watermelon seedlings. It is recommended that air temperature around grafted seedlings should be maintained at 27 to $29^{\circ}C$ for enhancing the physiological reaction of callus and for the smooth joining of scion and rootstock.

Highly Efficient Blue-Light-Emitting Diodes Based on Styrylamine Derivatives End-capped with a Diphenylvinyl Group

  • Kim, Seul-Ong;Lee, Kum-Hee;Kang, Sun-Woo;Lee, Jin-Yong;Seo, Ji-Hoon;Kim, Young-Kwan;Yoon, Seung-Soo
    • Bulletin of the Korean Chemical Society
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    • v.31 no.2
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    • pp.389-396
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    • 2010
  • In this paper, we reported the synthesis and electroluminescent properties of blue fluorescent styrylamine derivatives end-capped with a diphenylvinyl group. A new series of styrylamine derivatives have been synthesized via the Horner-Wadsworth-Emmons reaction. To explore electroluminescent properties of these molecules, multilayer organic lighte-mitting devices with the configuration of ITO/NPB/1-5 doped in MADN/Bphen/Liq/Al were fabricated. All devices exhibited blue emissions with good EL performances. Among those reported herein, the device using dopant 5 exhibited a maximum luminance of $24,000\;cd/m^2$ at 11.0 V, a luminous efficiency of 12.5 cd/A at $20\;mA/cm^2$, a power efficiency of 6.50 lm/W at $20\;mA/cm^2$, and $CIE_{x,y}$ coordinates of (x = 0.173, y = 0.306) at 8.0 V, all of which demonstrate the superiority of these materials in blue OLEDs.

Effect of LEDs (Light Emitting Diodes) Irradiation on Growth and Mineral Absorption of Lettuce (Lactuca sativa L. 'Lollo Rosa') (LED 광원이 상추의 생육 및 무기물 흡수에 미치는 영향)

  • Shin, Yong Seub;Lee, Mun Jung;Lee, Eun Sook;Ahn, Joon Hyung;Lim, Jae Ha;Kim, Ha Joong;Park, Hoo Won;Um, Young Ghul;Park, So Deuk;Chai, Jang Heui
    • Journal of Bio-Environment Control
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    • v.21 no.3
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    • pp.180-185
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    • 2012
  • The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Rollo Rosa'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red + blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red + blue and fluorescent light irradiation had a higher $a^*$ value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red + blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red + blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.

Effect of Light Emitting Diodes Treatment on Growth and Quality of Lettuce (Lactuca sativa L. 'Oak Leaf') (LED 처리가 상추의 생육 및 품질에 미치는 영향)

  • Shin, Yong-Seub;Lee, Mun-Jung;Lee, Eun-Sook;Ahn, Joon-Hyung;Kim, Min-Ki;Lee, Ji-Eun;Do, Han-Woo;Cheung, Joung-Do;Park, Jong-Uk;Um, Young-Ghul;Park, So-Deuk;Chae, Jang-Heui
    • Journal of Life Science
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    • v.24 no.2
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    • pp.148-153
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    • 2014
  • The objective of this study was to elucidate the effect of light-emitting diode treatment on early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Oak Leaf'). In changes to leaf morphology, shoot elongation and hypocotyl length showed poor growth under red light irradiation, while red+blue light irradiation induced shorter plant height and more leaves, resulting in increased fresh weight. With respect to Hunter's color and SPAD values, lettuce seedlings grown under red+ blue and fluorescent light irradiation had a higher $a^*$ value but showed no other changes to SPAD values. Interestingly, redness in relative chlorophyll content was 1.4 times higher under red+blue light irradiation. Inorganic element (N, Ca, Mg, and Fe) and ascorbic acid concentrations increased in lettuce plants grown under LED light irradiation compared to those of lettuce grown under fluorescent light, which showed a higher P content. In conclusion, red+blue light irradiation, which stimulates growth and higher nutrient uptake in leaf lettuce, could be employed in containers equipped with LEDs.

Effects of Selective Light Sources on Seedling Quality of Tomato and Cucumber in Closed Nursery System (폐쇄형 육묘시설 내에서 몇 가지 광원이 토마토와 오이의 묘소질에 미치는 영향)

  • Um, Yeong-Cheol;Jang, Yoon-Ah;Lee, Jun-Gu;Kim, Seung-Yu;Cheong, Seung-Ryong;Oh, Sang-Seok;Cha, Seon-Hwa;Hong, Seong-Chang
    • Journal of Bio-Environment Control
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    • v.18 no.4
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    • pp.370-376
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    • 2009
  • To produce uniform seedlings of tomato and cucumber with inexpensive way, their seedling quality by different light sources was investigated. The raising of seedling was performed by red LED (light emitting diodes), blue LED, red-blue mixed LED or fluorescent light with a fixed PPF(photosynthetic proton flux) level, about $40{\sim}60{\mu}mol{\cdot}m^{-2}{\cdot}sec^{-1}$. In the both tomato and cucumber, the rapid extension of hypocotyledonary axis was observed in Blue LED than fluorescent light, but opposite result was found in Red and mixed LED. During the nursery period of tomato and cucumber, the fresh weight was the highest in Red LED as 74% increasement in tomato and 74% in cucumber. In the case of seedling quality after the tomato nursery, there was no difference in the positions of 1st flower cluster and the number of bearing-flower per flower cluster by each light source. In case of cucumber, until 20th node, the setting ratio of female flower was higher in LED than fluorescent treatment, and also more healthy fruit setting was found in LED. Therefore, we assume that the Red or mixed (Red 2 + Blue 1) LED is more favorable to produce high quality tomato and cucumber seedlings in closed nursery facility.

Effects of Fluorescent Light and Light-Emitting Diodes on Leaf Morphology, Growth and Antioxidant Capacity of Salvia plebeia (형광등과 발광다이오드 광원이 '곰보배추'의 생육, 엽형 및 항산화능에 미치는 영향)

  • Park, Heon;Yu, Yeon Jung;Choi, Eun Young
    • Journal of Bio-Environment Control
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    • v.26 no.3
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    • pp.208-214
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    • 2017
  • This study aimed to determinate the effect of fluorescent light and light-emitting diodes on the leaf morphology, growth and antioxidant capacity of Salvia plebeia. The plants were grown for 56 days after transplanting (DAT) under the fluorescent light (FL) and LEDs (White, Red and Blue (R+B, ratio 2:1), Blue, Red LED) under the same light intensity and photoperiod ($130{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 12 hours). Leaf length, width and number of Salvia plebeia at the 56 DAT were significantly higher under the FL and red LED, and lower in the RB LED and white LED. The highest fresh and dry weights of shoot and leaf area were observed in the red LED, followed by the FL and blue LED, and the lowest in the RB LED and white LED. After 21 DAT, leaf apinasty symptom was appeared in plants grown under red LED and RB LED. The chlorophyll content was lower in the red LED. The specific leaf weight, the ratio of leaf dry weight to area, was higher in the blue LED, and lowest in the FL. No significant difference in DPPH radical scavenging activity of Salvia plebeia under the different light sources. All the integrated results suggest that the FL light is a proper light conditions for a closed cultivation of Salvia plebeia.

Emission Characteristics of White Organic Light-Emitting Diodes Using Blue Fluorescent and Red Phosphorescent Materials (청색 형광과 적색 인광 물질을 사용한 백색 OLED의 발광 특성)

  • Park, Chan-Suk;Ju, Sung-Hoo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.28 no.11
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    • pp.704-710
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    • 2015
  • We studied white organic light-emitting diodes using blue fluorescent and red phosphorescent materials. White single OLEDs were fabricated using SH-1 : BD-2 (3 vol.%) and CBP : $Ir(mphmq)_2(acac)$ (2 vol.%) as emitting layer (EML). The white single OLED using SH-1 : BD-2 (3 vol.% 8 nm) / CBP : $Ir(mphmq)_2(acac)$ (2 vol.% 22 nm) as emitting layer showed maximum current efficiency of 8.8 cd/A, Commission Internationale de l'Eclairage (CIE) coordinates of (0.403, 0.351) at $1,000cd/m^2$, and variation of CIE coordinates with ($0.402{\pm}0.012$, $0.35{\pm}0.002$) from 500 to $3,000cd/m^2$. The white tandem OLED using SH-1 : BD-2 (3 vol.% 12 nm) / CBP : $Ir(mphmq)_2(acac)$ (2 vol.% 18 nm) showed maximum efficiency of 19.6 cd/A, CIE coordinates of (0.354, 0.365) at $1,000cd/m^2$, and variation of CIE coordinates with ($0.356{\pm}0.016$, $0.364{\pm}0.002$) from 500 to $3,000cd/m^2$. Maximum current efficiency of the white tandem OLED was more twice as high as the single OLED. Our findings suggest that tandem OLED was possible to produce improved efficiency and excellent color stability.