• 식물병연구
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• 제13권2호
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• pp.88-92
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• 2007

우리나라 참다래 과수원에서 참다래 꽃썩음병을 일으키는 원인 세균인 Pseudomonas syringae pv. syringae를 분리하였다. 총 41개 균주 중 스트렙토마이신 저항성을 보이는 2개의 균주를 대상으로 PCR과 염기서열 결정을 통해 저항성 유전자 구조를 조사하였다. PCR결과 스트렙토마이신 저항성유전자는 Tn5359a에 들어 있는 strA-strB 구조인 것으로 밝혀졌다. Xanthomonas campestris와 Erwinia amylovora에서 알려진 IS6100과 IS1133은 발견되지 않았다. strA-strB의 염기서열은 이미 밝혀진 Tn5393a와 동일하였다. 두 스트렙토마이신 저항성 균주는 각각 3개의 플라스미드를 가지고 있었으며 저항성 유전자는 그 중 100kb의 플라스미드에 들어 있었다.

• Journal of Periodontal and Implant Science
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• 제27권4호
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• pp.867-882
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• 1997

Safflower(Carthamus tinctorius $Linn\acute{e}$ has been traditionally used for the treatment of blood stasis, and Dipsasi Radix has been used as a drug for fracture in Chinese medicine. The purpose of present study was to examine the biologic effects of safflower extract and Disasi radix extracts on the periodontal. ligament cells and osteoblastic cells and on the wound healing of rat calvarial defect. The ethanolic extract of safflower blossom, safflower seed and Dipsasi Radix(125, 250, and 500 ${\mu}g/ml$) were prepared as test group, and PDGF-BB(lOng/ml) and unsafonifiable fraction of Zea Mays L.(125, 250, and 500 ${\mu}g/ml$) were employed as positive control. The effects of each agents on the growth and survival, ALPase activity, expression of PDGF-BB receptor, chemotactic response of PDL cell and ATCC human osteosarcoma MG63 cells in vitro were examined. The tissue regenerative effect of each extracts was evaluated by histomorphometric measuring of newly formed bone on the 8mm defect in rat calvaria after oral administration of 3 different dosages groups : 0.02, 0.1 and 0.35g/kg, per day. It was also employed the same dosages of unsaponifiable fraction of Zea Mays L. as positive controls. Safflower blossom extract, safflower seed extract, and Dipsasi Radix extract stimulate the cellular activity of MG63 cells in concentration range of $125-500{\mu}g/ml$, and safflower bolssom extract and safflower seed extract stimulate also the cellular activity of periodontal ligament cells in concentration range of $250-500{\mu}g/ml$. In activity of ALPase, $250-500{\mu}g/ml$ of safflower blossom extracts showed significant stimulating effects on MG63 cells, and the same concentration range of safflower seed extracts showed significant effect on periodontal ligament cells. In the recovery on PDGF-BB receptor expression which was depressed by $IL-1{\beta}$, $125-250{\mu}g/ml$ of safflower blossom extracts and $250-500{\mu}g/ml$ of safflower seed extracts showed significant increasing effect on MG63 cells, and $500{\mu}g/ml$ of safflower blossom extract and $250-500{\mu}g/ml$ of safflower seed extracts showed significant effect on periodontal ligament cells. In chemotactic response, among all tested group, safflower seed extracts only were chemotactic to MG63 cells and periodontal ligament cells in concentration range of $125-500{\mu}g/ml$. Also in the view of bone regeneration in rat calvarial defect model, the only group that was orally administrated 0.35g/kg, day of safflower seed extract showed significant new bone formation. These results suggested that safflower extracts might have a potential possibilities as an useful drug for adjunct to treatment for regeneration of periodontal defect.

• 한국환경과학회지
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• 제23권5호
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• pp.781-786
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• 2014

Growth and physiological disorders caused by abnormally low temperatures were evaluated in pepper, an important field crop in Korea. In addition, the effects of chemical treatment using glutamine was verified on minimizing the damages by low temperature. The growth of pepper plants in stem length and diameter was suppressed as the temperature decreased from $25^{\circ}C$, and the suppression level was the highest for plants grown for 90 days at $20^{\circ}C$. However, root growth was not affected by the different temperatures. The number of leaf and leaf area decreased at the temperatures below $25^{\circ}C$, an optimum temperature for growth. Fresh weight and dry weight decreased for plants grown at $20^{\circ}C$. Pepper fruit yield also decreased by 11% at $20^{\circ}C$ in comparison to $25^{\circ}C$. Falling blossom rate was different depending on the growth temperature, and the rate was 27.2% at $25^{\circ}C$, 35.2% at $22.5^{\circ}C$, and 41.0% at $20^{\circ}C$, indicating that falling blossom rate increased as temperature decreased. Different growth temperatures did not affected on the level of symptom of calcium deficiency and Phytopathora blight. Falling blossom was severe at abnormally low temperature of $20^{\circ}C$, but the treatment of glutamine reduced falling blossom rate and increased the yield by 7.0% as compared to control. The optimum concentration of glutamine treatment was 10 mg/L for yields.

• 원예과학기술지
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• 제33권4호
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• pp.486-491
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• 2015

본 연구에서는 조생종 '한아름'과 중생종 '신고'에서 만개전 적화처리가 과실의 비대 및 품질에 미치는 영향을 조사하기 위하여 만개 전 5일에 적화처리를 실시하였다. 처리내용은 무처리구, 기부로부터 1, 2번화 제거구(T1), 6, 7번화 제거구(T2)로 나누어 실시하였다. 조생종 '한아름'의 경우 최종수확기에는 3.6%(T1) 및 4.6%(T2)의 크기 증가를 보였고 수확기 과실무게도 10.5%(T1) 및 11.0%(T2)의 증가를 보였다. 중생종 '신고'에서는 최종 수확기에는 12.6%(T1) 및 16.8%(T2)의 과실무게 증가를 보였고 두 품종 모두 대과의 생산비율이 증가하는 경향을 보였다. 적화처리구는 수확기 가용성고형물 함량이 $1^{\circ}Brix$ 정도 증가하였으며 '신고'의 경우에도 무처리구에 비해 적화처리구의 과실에서 높은 적색도를 보였다. 따라서 조생종 '한아름'과 중생종 '신고' 배에 대한 만개 전 적화 처리는 과실의 비대촉진 및 가용성 고형물 증가 및 과피색 향상에 효과를 보이는 것으로 조사되었으며 화총의 기부로부터 6, 7번째 착색된 꽃을 제거하는 것이 보다 실용적인 방법으로 추천되었다.

• 한국환경농학회지
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• 제39권4호
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• pp.360-367
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• 2020

BACKGROUND: The productivity and quality of blueberry (Vaccinium ashei Reade) greatly depend on the number of fruits in a plant. Especially, fruit set more than appropriate number negatively affects productivity and marketability due to the increased number of small fruits and delayed harvest time. This study was conducted to investigate proper timing and concentration for applying chemical blossom thinners such as ammonium thiosulfate (ATS) and UREA. METHODS AND RESULTS: ATS at 1.25% and 1.50%, and UREA at 6% and 8% were applied in four developmental stages, bud swell, pink bud, full bloom and petal fall. Fruit thinning rate was calculated based on the number of fruits harvested divided by that of flowers before applying blossom thinners. Ratios of leaf to flower and leaf to fruit were calculated based on the number of fully developed leaves in 25 days after full blossom divided by that of flowers or fruits, respectively. Chemical injury of leaves was investigated by calculating the number of leaves with chemical injury divided by the total number of leaves. Fruit thinning rates were 48% and 66% for UREA treatments at 6% and 8%, respectively, and 49% and 62% for ATS treatments at 1.25% and 1.50%, respectively, in the full bloom stage. In the petal fall stage, fruit thinning rates were 18% and 24% for UREA treatments at 6% and 8%, respectively, and 49% and 35% for ATS treatments at 1.25% and 1.50%, respectively. Leaf to fruit ratio (L/FR) increased by 109% and 188% compared to leaf to flower ratio in ATS treatments at 1.25% and 1.50%, respectively, and L/FR increased 93 and 196% in UREA treatments at 6% and 8%, respectively, in the full bloom stage. In the petal fall stage, leaf to fruit ratio increased by 60% to 100% in ATS treatments, but did not significantly differ from the control in UREA treatments. Fruit harvest was delayed in all treatments of all developmental stages except for 1.5% ATS and 6% UREA treatments at the petal fall stage, whose fruit harvest was two or three days faster than the control. CONCLUSION: The application of ATS and UREA for blossom thinning should be in the petal fall and full bloom stages for early and late harvest, respectively. Considering chemical injury, integrated harvesting and fruit size, however, it is appropriate to apply ATS at 1.5% in the petal fall stage to increase fruit productivity and quality in blueberry.

• 한국농림기상학회지
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• 제24권4호
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• pp.305-317
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• 2022

배 화상병의 성공적 방제를 위해 2018년부터 2022년까지 우리나라 중부지방의 주요 발병지와 남부지방의 미발병 주산지 주요지점 25곳에 대한 Maryblyt를 구동하여 꽃감염 위험도를 조사하였다. 최근 5년 중 2019년과 2022년 개화기간 중 꽃감염 위험도가 가장 높았다. 한편, 개화기간 중 최적의 꽃감염 방제 처리는 High 경보 다음날에 방제하고, 강우예보를 발령한 전날 방제하는 처리가 배 꽃감염을 낮추는 것으로 평가하였다. 월동 궤양으로부터 활성화된 궤양이 병징을 보일 것으로 Maryblyt가 예측한 날은 대략 중부지방 기준 5월 중순이었는데 이때부터 현장에서 궤양 모니터링을 개시하도록 권장하였다. 천안, 이천, 상주, 나주 등 4곳의 배 과수원에 설치한 영상자료로부터 배 개화기간을 이론적으로 계산한 값과 실제 관측한 값의 차이점을 비교한 결과 남부지방은 이론치나 실측치보다 늘 빠르게 개화를 예측하므로 재조정이 필요하였다. 향후 현장 관리자와 농민들로부터 과원에서 관측한 기상, 기주인 과수, 병징 출현일 등의 정보들이 축적된다면 발병 예측 모델은 현재보다 더 정확한 정보를 제공할 수 있을 것으로 기대된다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 임시총회 및 추계 학술발표회
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• pp.62.2-62
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• 1999

• 생물환경조절학회지
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• 제13권4호
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• pp.194-199
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• 2004

염류집적 토양에서 광합성균, Soil plus, 목초액, 활성탄 등 토양개량제가 오이와 토마토의 생육과 수량에 미치는 영향을 시험하였다. 그 결과 활성탄이 수량을 증진시키고 뿌리의 활력도 좋았다. 토마토 배꼽썩음병도 활성탄 처리구가 가장 낮았다. 토마토의 당도는 Soil plus구가 높았으며 질산염과 인산함량은 처리간에 차이가 없었다.

• 농업과학연구
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• 제47권1호
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• pp.67-81
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• 2020

The physicochemical parameters of honey are used to determine the botanic origin of honey and to specify the composition criteria for honey in regulations and standards. The parameters of honeydew and blossom honeys from Korean beekeepers were determined to investigate whether they complied with the composition criteria for honey in the food code legislated by Korean authority and to establish the parameters which should be subjected to principal component analysis for improved differentiation of honeys. The fructose and glucose contents of the honeydew honey did not comply with the composition criteria. The ash content of the honey was closely correlated with CIE a^* and CIE L^* The principal component analysis of fructose to glucose ratio, CIE a^*, CIE L^*, ash content, free acidity, and fructose and glucose contents enabled classification of honeydew, chestnut, multifloral, and acacia honeys. Additional advantage of the principal component analysis (PCA) is that the physicochemical parameters, such as fructose to glucose ratio (F/G) and color, can be determined using the analytical instruments for composition criteria and quality control of honey. This study suggested that composition criteria for honeydew honey should be established in the food code in accordance with international standards. The principal component analysis reported in this study resulted in improved classification of the honeys from Korean beekeepers.

• 한국의류학회지
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• 제38권2호
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• pp.201-215
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• 2014

This study explores the kinds and types of dyes used in the Joseon Dynasty. Some literature including "Sanga Yorok", "Gyuhap Chongseo", "Imwon Gyeonjeji" were reviewed for this purpose. The findings are as follows. The colors obtained from the dyes were divided into seven color series, such as red, yellow and orange, green, blue, purple, brown, gray and black. These are practical color categories differed from abstract five cardinal colors [五方色]. The diachronic dyes used during the Joseon Dynasty were safflower and Japanese alder bark for red, indigo leaves for blue, gromwell for purple, chestnut tree bark for brown. The representative dyes used in the late Joseon Dynasty were safflower and sappan wood for red, wild pear tree bark and Pentapetes phoenicea for yellow and orange, indigo leaves for blue, sappan wood and gromwell for purple, mulberry tree for brown, and Chinese ink for gray. Common dyes used both in Joseon and China were safflower and sappan wood for red, pagoda tree blossom for yellow, a combination of pagoda tree blossom (or Amur cork tree) and indigo leaves (or sediment) for green, indigo leavers or sediment for blue, sappan wood and hollyhock for purple, and a combination of indigo leaves and Galla Rhois for gray and black.