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http://dx.doi.org/10.5423/RPD.2007.13.2.088

Streptomycin Resistant Genes of Pseudomonas syringae pv. syringae, the Causal Agent of Bacterial Blossom Blight of Kiwifruit  

Park, So-Yeon (Department of Biology, Sunchon National University)
Han, Hyo-Shim (Department of Biology, Sunchon National University)
Lee, Young-Sun (Department of Biology, Sunchon National University)
Koh, Young-Jin (Department of Plant Medicine, Sunchon National University)
Jung, Jae-Sung (Department of Biology, Sunchon National University)
Publication Information
Research in Plant Disease / v.13, no.2, 2007 , pp. 88-92 More about this Journal
Abstract
A total of 41 Pseudomonas syringae pv. syringae, the causal agent of bacterial blossom blight, were isolated from kiwifruit plants in Korea. Among them, two strains showing streptomycin resistance were examined to investigate the structure of resistant determinants by PCR and nucleotide sequence analysis. PCR results suggested that the streptomycin resistance is mediated by strA-strB genes carried on Tn5393a. Insertion sequences, IS6100 and IS1133, which were located within or downstream of tnpR gene in Xanthomonas campestris and Erwinia amylovora were not found. Nucleotide sequences of strA-strB were 100% identical with Tn5393a. Two stretomycin resistant strains had three plasmids. Southern blot hybridization using strA-strB probe indicated that the resistant genes were carried on a 100kb plasmid.
Keywords
Blossom blight; Kiwifruit; Pseudomonas syringae pv. syringae; Streptomycin resistance;
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