• ALGAE
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• v.37 no.4
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• pp.281-291
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• 2022

Quantifying the abundance of Chattonella species is necessary to effectively manage the threats from ichthyotoxic raphidophytes, which can cause large-scale mortality of aquacultured fish in temperate waters. The identification and cell counting of Chattonella species have been conducted primarily on living cells without fixation by light microscopy because routine fixatives do not retain their morphological features. Species belonging to the Chattonella marina complex, including C. marina and C. marina var. ovata, had high genetic similarities and the lack of clear morphological delimitations between the species. To estimate the abundance of C. marina complex in marine plankton samples, we developed a protocol based on the droplet digital polymerase chain reaction (ddPCR) assay, with C. marina complex-specific primers targeting the internal transcribed spacer (ITS) region of the rDNA. Cell abundance of the C. marina complex can be determined using the ITS copy number per cell, ranging from 25 ± 1 for C. marina to 112 ± 7 for C. marina var. ovata. There were no significant differences in ITS copies estimated by the ddPCR assay between environmental DNA samples from various localities spiked with the same number of cells of culture strains. This approach can be employed to improve the monitoring efficiency of various marine protists and to support the implementation of management for harmful algal blooms, which are difficult to analyze using microscopy alone.

• Journal of Sensor Science and Technology
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• v.33 no.1
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• pp.30-33
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• 2024

The occurrence of harmful algae on the coast of Korea has been a cause of damage to the aquaculture industry and deterioration of the coastal ecosystem environment. A method is required to predict their outbreak in real-time at the site. Therefore, this study attempted to develop a small hybrid optical sensor and real-time monitoring system based on LiDAR that can be used in the field and laboratory and can be applied to various platforms. FMS-L specifically suggested the amount of Chlorophyll a (Chl a) in the sample by measuring and analyzing the fluorescence emitted by the irradiating light. The accuracy of FMS-L was verified by measuring the concentrations of standard Chlorophyll a substances and Margalfidinium polykirkoids. In addition, the precision was verified by comparing the measurement results of FMS-L using commercial equipment Phyto-PAM-II. This equipment is compact and easy to move. Therefore, it can be easily applied to field surveys, allows short time measurements (10 s), and can be applied at a distance of 10 m from the measurement site.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38C no.3
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• pp.263-270
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• 2013

Recently, the prediction and analysis technology of marine environment are actively being studied since the ocean resources in the world is taken notice. The prediction of marine disaster by automatic collecting marine environment data and analyzing the collected data can contribute to minimized the damages with respect to marine pollution of oil spill and fisheries damage by red tide blooms and marine environment upsets. However the studies of the marine environment monitoring and analysis system are limited in South Korea. In this paper, we study the marine disasters prediction system model to analyze collection marine information of out sea and near sea. This paper proposes the models for the marine disasters prediction system as communication system model, a marine environment data monitoring system model, prediction and analyzing system model, and situations propagation system model. The red tide prediction model and summarizing and analyzing model is proposed for prediction and analyzing system model.

• Journal of Korean Society on Water Environment
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• v.26 no.2
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• pp.231-242
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• 2010

The objective of study was to determine an optimal location of a float-type curtain weir in Daecheong Reservoir and to assess its effectiveness for the control of algal blooms in the reservoir. CE-QUAL-W2, a laterally averaged two-dimensional hydrodynamic and eutrophication model, was modified to accommodate vertical displacement of the weir according to water surface fluctuation and applied to simulate the reservoir hydrodynamics and water quality changes for the reservoir. The model calibrated in a previous study was updated and validated for different hydrological conditions representing drought year (2008) and normal year (2006) for the study, and adequately simulated the temporal and spatial variations of water temperature, nutrients and algal (Chl-a) concentrations. The effectiveness of curtain weir on the control of algal bloom was evaluated by applying the validated model to 2001 and 2006 assuming 9 scenarios for different installation locations. The reduction rates of algal concentration were placed in the range of 11.2~40.3% and 20.3~56.7% for 2001 and 2006, respectively. Although, the performance of curtain weir was slightly varied for different locations and different hydrological years, overall, the performance was improved as the weir was installed further downstream.

• 한국해양학회지
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• v.23 no.4
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• pp.159-168
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• 1989

Nutrients and trace metals of copper and nickel were investigated in the inner part of Deukryang Bay (southern coast of Korea) during 1980-81. This region is characterized as permanently well-mixed coastal waters. Because of this hydrographic condition, nutrients and trace metals showed interesting features in their seasonal cycles. The concentrations of dissolved nutrients and dissolved trace metals were higher in fall and winter than in spring and summer, while the concentrations of suspended particulates and particulate trace metals were much higher in winter than the rest of the year. Seasonal changes of nutrients suggest that the addition of nutrients via freshwater runoff during the late summer enhanced phytoplankton growth and subsequently caused phytoplankton blooms in winter. Depletion of nitrate seems to terminate phytoplankton bloom in this region, and copper and nickel appeared to be controlled by the in situ primary production.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.139-148
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• 2015

BACKGROUND: The aim of this study was to isolate and identify algicidal bacterium that tends to kill the toxic dinoflagellate Alexandrium catenella, and to determine the algicidal activity and algicidal range of algicide. METHODS AND RESULTS: Among of algicidal bacteria isolated in this study, NH-3 isolate was the strongest algicidal activity against A. catenella. NH-3 isolate was identified on the basis of biochemical characteristics and analysis of 16S rRNA gene sequences. The NH-3 isolate showed over 99% homology with Arthrobacter oxydans, and was designated as Arthrobacter sp. NH-3. The optimal culture conditions were $25^{\circ}C$, initial pH 7.0, and 2.0% (w/v) NaCl concentration. The algicidal activity of Arthrobacter sp. NH-3 was significantly increased to maximum value in the late of logarithmic phase. Arthrobacter sp. NH-3 showed algicidal activity through indirect attack, which excreted active substance into the culture filtrate. When 10% culture filtrate of NH-3 was applied to A. catenella, 100% of algal cells were destroyed within 30 h. In addition, the algicidal activities were increased in dose and time dependent manners. The pure algicide was isolated from the ethyl acetate extract of the culture filtrate of NH-3 by using silica gel column chromatography and high performance liquid chromatography (HPLC). We investigated the algicidal activity of this algicide on the growth of harmful algal bloom (HAB) species, including A. catenella. As a result, it showed algicidal activity against several HAB species at a concentration of $100{\mu}g/mL$ and had a relatively wide host range. CONCLUSION: Taken together, our results suggest that Arthrobacter sp. NH-3 and its algicide could be a candidate for controlling of toxic and harmful algal blooms.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.51 no.1
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• pp.128-135
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• 2015

The worldwide abundance of various jellyfish appears to have increased in coastal ecosystems in recent years. The enormous jellyfish blooms cause a variety of problems for the local ecology, fisheries, and aquatic-sports in coastal locations. In this study, jellyfish sting protection net was installed to ensure the safety and reduction of the inflow into the Haeundae beach. In order to confirm the stability of the protection net, the tension for protection net was measured from variation of current speed. The periods for maximum tension were observed correspond to the periods of maximum current speed. The maximum tension for protection net was measured up to 4,100 kg. From field evaluations, the jellyfish sting protection net has demonstrated to stability from the current and tide in the Haeundae beach.

• Journal of Ocean Engineering and Technology
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• v.23 no.2
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• pp.18-27
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• 2009

This study was conducted to examine the effect of wind on the circulation of seawater in Gamak Bay, which contains numerous farms for fish and shellfish but suffers a significant loss by fisheries nearly annually from harmful algal blooms. In numerical experiments with a simplified bathymetry for Gamak Bay, the wind in summer appeared to more strongly influence the east-westward flow than the south-northward flow. In winter, this trend was nearly similar to the summer but seemed to have a greater effect on the flow at the north-west of the bay than the flow at the south mouth of the bay. On the other hand, in numerical experiments with a realistic bathymetry for Gamak Bay, the wind in summer appeared to more strongly influence the east-westward flow than the south-northward flow. Furthermore, the effect of the wind was stronger at the south mouth of the bay than at the north-west of the bay. In contrast, the wind in winter affected the east-westward flow more strongly and its effect appeared stronger at the north-west of the bay than at the south mouth of the bay. In addition, the effect of the wind tended to increase with distance from the east to the west. Therefore, the tidal currents in Gamak Bay proved to be strongly influenced by the wind, in particular east-westward. However, some measures are urgently required to improve the water quality of the bay, since the south-northward flow turned out to be obstructed by an east-westward shoal located in the middle of the bay.

• ALGAE
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• v.23 no.1
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• pp.15-29
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• 2008

Many species in Gymnodiniales, which are unarmored dinoflagellates, are responsible for marine algal blooms and some of them have potent toxin in the cell. Their taxonomy has so far been well-defined, and several genera (e.g. Akashiwo, Gymnodinium, Karenia) have recently been re-described. In Korea, few works have been carried out on their taxonomical and molecular studies. This study focused on comparison of both morphological and molecular characteristics of five unarmored dinoflagellates on Korean coastal water: Akashiwo sanguinea, Cochlodinium polykrikoides, Gymnodinium catenatum, Gymnodinium impudicum and Karenia aureolum (=K. mikimotoi). Morphological characteristics observed here was in good accordance with the original descriptions of individual species. In addition, none of difference was found in morphological comparisons between the Korean and foreign strains. Furthermore, molecular analysis showed that the SSU rDNA sequences were generally identical according to each species. In some distinct features, A. sanguinea, which has generally the same morphological features, were divided into two groups: one was Korean isolates including European isolates, the other was American isolates. In the two groups, the nucleus was positioned differently: middle of the cells in the Korean isolates (GnSg02, GnSg03), near the epicone in American isolates (CCMP1593, CCMP1837). In addition, this was strongly supported by phylogenetic analysis, inferred from the SSU rDNA sequences. K. aureolum (GrAr01) was corresponded to European G. aureolum (=K. mikimotoi) in shape and position of nucleus, chloroplast, however, which is similar to K. digitata in view of having a finger-like sulcus. This was in good agreement with phylogenetic study of these species. G. catenatum have identical morphology except the ridge location, and their genotype of SSU rDNA was also identical to GenBank data of the same species. From this study, we found that the five Korean unarmored dinoflagellates are identical morphological characteristics and genotype to each species of foreign isolates.

• ALGAE
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• v.25 no.3
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• pp.141-153
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• 2010

Seagrass epiphyte blooms potentially have important economic and ecological consequences in Tampa Bay, one of the Gulf of Mexico's largest estuaries. As part of a Tampa Bay pilot study to monitor the impact of environmental stresses, precise characterization of epiphyte diversity is required for efficient management of affected resources. Thus, epiphyte diversity may be used as a rational basis for assessment of ecosystem health. In May 2001, epiphytic species encompassing green, brown and red macroalgae were manually collected from dense and sparse seagrass beds of Thalassia testudinum and Syringodium filiforme. A total of 20 macroalgal epiphytes, 2 Chlorophyta, 2 Phaeophyta, and 16 Rhodophyta, were found on T. testudinum and S. filiforme seagrass at the four sampling sites (Bishop Harbor, Cockroach Bay, Feather Sound, and Mariposa Key). The Rhodophyta, represented by 16 species, dominated the numbers of species. Among them, the thin-crusted Hydrolithon farinosum was the most commonly found epiphyte on seagrass leaves. Species number, as well as species frequency of epiphytes, is higher at dense seagrass sites than sparse seagrass sites. Four attachment patterns of epiphytes can be classified according to cortex and rhizoid development: 1) creeping, 2) erect, 3) creeping & erect, and 4) erect & holding. The creeping type is characterized by an encrusting thallus without a rhizoid or holdfast base. Characteristics of the erect type include a filamentous thallus with or without a cortex, and a rhizoid or holdfast base. The creeping and erect type is characterized by a filamentous thallus with a cortex and rhizoid. A filamentous thallus with a cortex, holdfast base, and host holding branch is characteristics of the erect and holdfast attachment type. This study characterized each species found on the seagrass for epiphyte identification.