• Parasites, Hosts and Diseases
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• 제56권1호
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• pp.53-59
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• 2018

Tick saliva is critically important for continuous attachment to the host, blood feeding for days, and transmission of tick-borne pathogens. To characterize the patterns of inflammatory cytokine gene expression during its attachment and blood sucking time, peripheral blood samples of rabbits infested with Haemaphysalis longicornis ticks were collected at different intervals. Blood histamine concentration was evaluated as well as gene encoding IFN-${\gamma}$, TNF-${\alpha}$, IL-2, IL-6, IL-4, and IL-10 were compared with non-infested rabbits. Blood histamine concentration of tick-infested rabbits during fast feeding time was significantly higher than that of non-infested rabbits. In both nymph and adult tick infested rabbits, expression of TNF-${\alpha}$ and IFN-${\gamma}$ genes were decreased significantly (P<0.05), while expression of IL-4, IL-6, and IL-10 were increased 1.3 to 7 folds in adult infested rabbits with the exception of IL-6 that was significantly (P<0.05) decreased in nymph infested rabbits. IL-2 was not expressed in either nymph or adult infestation. H. longicornis saliva is capable of modulate host responses through a complex correlation with histamine and Th1, Th2 mediated cytokines that suppress the inflammatory responses directed toward inflammatory mediators introduced into the host during tick feeding.

• Parasites, Hosts and Diseases
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• 제52권5호
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• pp.507-511
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• 2014

Outbreaks of tick-borne disease cases in Santa Catarina, Brazil are known, but the presence of the pathogen DNA has never been determined. In this study, the first survey of Anaplasma marginale, Babesia bigemina, and Babesia bovis DNA on blood samples of 33 cattle from an outbreak in Ponte Alta Municipality, Santa Catarina, Brazil, has been carried out. A multiplex PCR detected 54.5% of animals were co-infected with 2 or 3 parasites, while 24.2% were infected with only 1 species. The most prevalent agent was B. bigemina (63.6%) followed by A. marginale (60.6%). This is the first report of tick-borne disease pathogens obtained by DNA analysis in Southern Brazil.

• Journal of Microbiology
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• 제42권3호
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• pp.239-242
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• 2004

This study examined the detection rate of the hepatitis B virus (HBV) and Mycobacterium tuberculosis (Mtb) in serum and saliva samples, respectively, from 120 dental patients who were unaware if they have or had either hepatitis or tuberculosis. The frequencies of HBsAg and anti-HBs were determined using an immunochromatic assay. Mtb positivity was determined by the PCR method. Of the 120 patients, 7 (5.8％) were HBV positive and 30 (25.0％) were Mtb positive. This highlights the fact that dental health care workers (DHCWs) can be exposed to the risk of infection from blood- or saliva-borne pathogens as a consequence of their work. Therefore, it is very important to prevent cross infection between patients and dental personnel. Accordingly, laboratory tests prior to surgical treatment are needed to determine the infectious state of dental patients in order to prevent the transmission of infectious diseases in dental clinics.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제41권5호
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• pp.281-283
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• 2015

Extraction of an impacted third molar is one of the most frequently performed techniques in oral and maxillofacial surgery. Surgeons can suffer numerous external injuries while extracting a tooth, with percutaneous injuries to the hand being the most commonly reported. In this article, we present a case involving a percutaneous injury of the surgeon's femoral region caused by breakage of the fissure bur connected to the handpiece during extraction of the third molar. We also propose precautions to prevent such injuries and steps to be undertaken when they occur.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.481-489
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• 2017

Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015. Cattle that grazed in mountainous areas showed a greater prevalence of tick-borne infections with an increased Theileria orientalis infection rate (54.7%) compared to that in non-grazing cattle (16.3%) (P<0.001). Accordingly, the red blood cell (RBC) count and hematocrit (HCT) values of grazing cattle were significantly lower than those of non-grazing cattle throughout the season (P<0.05). Moreover, RBC, hemoglobin (Hb), and HCT of T. orientalis-positive group were significantly lower than those of T. orientalis-negative group (P<0.05). T. orientalis is a widespread tick-borne pathogen in Korea. Grazing of cattle in mountainous areas is closely associated with an increase in T. orientalis infection (RR=3.4, P<0.001), and with consequent decreases in RBC count and HCT. Thus, these findings suggest that the Hanwoo cattle in mountainous areas of Korea are at a high risk of infection by T. orientalis, which can lead to hematological alterations. This study highlights the necessity of preventive strategies that target T. orientalis infection.

• 성인간호학회지
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• 제22권5호
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• pp.466-476
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• 2010

Purpose: The purpose of this study was to examine the under-reporting rate and related factors after blood and body fluid (BBF) exposure among hospital employees. Methods: Fifteen hundred l employees were conveniently sampled from ten university and acute care hospitals. The survey questionnaire consisted of 37 items. Data were collected from September 10 to November 30, 2008. Results: The survey response rate was 88.7%. The 47.9% (638/1,331) of hospital employees were exposed to BBF and the mean number of exposure was $4.7{\pm}5.942$ within the previous year. Under-reporting rate after BBF exposure was 69.4% (443/638). By multi-variate logistic regression analysis, the exposure number, exposure type, infectious disease and hospital were independently related to the under-reporting of BBF among hospital employees. Conclusion: The under-reporting rate after being exposed to blood and body fluids was relatively high. To address this problem, educational programs are needed to decrease the under-reporting rate for healthcare workers. Further, it might be helpful if other factors related to under-reporting be investigated in future studies.

• 한국임상수의학회지
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• 제32권5호
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• pp.473-475
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• 2015

시골에 사는 12년령의 중성화 안한 수컷 말라뮤트가 회음부위의 염증과 통증을 주호소로 내원하였다. 회음부 신체검사에서 구더기와 구멍이 있는 피부 병변이 존재하였다. 혈액검사에서는 미약한 빈혈과 혈소판 증가증 이외에 특이소견은 보이지 않았으며 진단은 임상증상 및 구더기의 존재에 의해 쉽게 되었다. 즉시 삭모 및 소독을 실시하였으며 삭모 중 진드기가 발견되어 SNAP 4DX (IDEXX Laboratories, Westbrook, ME, USA)를 이용해 진드기 및 관련 병원체의 감염유무를 평가하였고 검사결과는 모두 음성이었다. 치료는 이버멕틴(300 mcg/kg)을 피하 주사로 한번 주사하였으며 2차 감염을 예방하기 위해 항생제를 처방하였다. 이 후 구더기증과 상처부위는 2주 이내에 치료되었다.

• 한국임상수의학회지
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• 제28권2호
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• pp.204-210
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• 2011

국내 독일 셰퍼드로부터 에를리히아증과 라임병에 대한 항체가 조사를 위하여 효소면역측정법을 시용하였으며, 중합효소연쇄반응을 이용하여 진드기매개성 병원체를 조사하였다. 2005년 10월부터 2006년 9월까지 국내 5개 지방으로부터 291마리 독일 셰퍼드를 선정하였다. 개 에를리히아 항체 조사를 위한 ELISA (Snap$^{(R)}$ 3Dx$^{(R)}$ test kit, IDEXX) 검사결과 전국적으로 7.56%(22마리)의 에를리히아 양성 항체 보균개와 1.72%(5마리)의 보렐리아 양성 항체 보균개로 조사되었다. 이 중 2마리에서는 두 가지 병원체 모두에 대한 양성 항체가 검출되었다. 에를리히아에 대한 양성항체를 지방별로 보면 경기도는 1.28%(78마리 중 1마리), 강원도는 12.64%(87마리 중 11마리), 충청도는 9.76%(41마리 중 4마리), 경상도는 8.93%(56마리 중 5마리), 전라도는 3.45%(56마리 중 5마리)로 조사되었다. Ehrlichia chaffeensis 유전자를 검출한 결과 전국 평균이 3.09%(291 마리 중 9마리), 강원도는 2.41%(291 마리 중 7마리) 그리고 충청도는 0.69%(291마리중 2마리)로 조사되었다. 중합효소연쇄반응으로 증폭된 유전자(SNU-EC3과 SNU-EC5)에 대한 유전자염기서열분석결과 한국(Haemaphysalis longicornis 진드기)과 중국 그리고 Arkansas 분리주인 E. chaffeensis와 매우 유사하였다. 이 연구의 결과 국내에서 사육되는 독일셰퍼드에서 에를리히아증의 병원체와 라임병에 대한 항체가 검출된 것으로 볼 때 임상 진단에 있어서 진드기매개전염병에 대한 중요성을 인식해야 할 것으로 판단된다. 따라서 혈소판감소증이나 백혈구감소증이 있는 경우 그리고 봄부터 가을까지 야외활동을 한 경력이 있는 환자에 대한 감별진단 항목에 반드시 포함시켜야 할 것을 제안한다.

• Journal of Trauma and Injury
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• 제28권1호
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• pp.9-14
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• 2015

Purpose: The aim of this study was to estimate the seropositive prevalence of blood-borne infection in neurotrauma patients who underwent emergent surgical intervention, especially patients with hepatitis B virus (HBV), hepatitis C virus (HCV), syphilis and human immunodefIciency virus (HIV). Methods: A retrospective review identified 559 patients with traumatic brain injury and spinal trauma who underwent emergent surgery between 2007 and 2014. We reviewed the medical records and extracted data, including age, sex, location of lesion, result of serologic tests, time interval of admission and surgery after presenting to emergency room. Serologic tests for HBV, HCV, syphilis and HIV were performed and analyzed to determine whether the seropositive results were confirmed by the surgeon before surgery. Results: The majority of the patients were male (74.6%), and the mean age was $55.4{\pm}20.2years$. Most patients underwent surgery due to traumatic brain injury (90.0%). Fifty-three patients (10.0%) showed a positive result on at least one serologic test. Seropositive rates according to pathogens were 0.5% for syphilis, 5.2% for HBV and 3.9% for HCV. No positive results were noted on the serologic tests for HIV. HBV in patients with spinal cord injury and age from 40 to 49 years were associated with high serologic positive rate, and that result was statistically significant. However, no statistically significant differences were found in the other variables. Serologic results could not confirmed before surgery in the majority of the cases (62.1%), and 10.4% of these patients showed seropositive results. Conclusion: The results of this study emphasize the importance of taking precautions and conducting rapid serologic testing in preventing the occupational transmission of blood-borne viruses to health-care workers.

• 한국임상수의학회지
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• 제31권1호
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• pp.6-10
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• 2014

제주도에 서식하는 노루는 육지부 다른 종으로 대부분이 진드기에 심하게 감염되어 있다. 제주노루가 진드기 매개질병에 감염 및 다른 야생동물이나 가축에게 질병을 전파할 수 있다. 이에 본 연구에서는 제주야생동물구조센에서 구조 및 치료되는 제주노루 23마리의 혈액과 진드기를 대상으로 진드기 종을 동정하고 주혈원충 감염을 확인하였다. 구조된 노루의 대부분은 심하게 진드기에 감염되었고 모두가 작은소참진드기(100%)였다. 감염노루 혈액과 진드기를 대상으로 주혈원충에 대한 PCR, RFLP 및 염기서열 분석한 결과 혈액시료 모두(100%)에서 Theileria luwenshuni로 확인되었으며, 진드기에서는 23개군중 8개군(34.8%)에서 확인되었다. 제주 노루 감염 진드기는 주로 작은소참진드기며, 이들이 T. luwenshuni 매개함을 확인하였다. 그러나 감염에 따른 혈액 및 혈청학적 검사에서 정상소견과 유의적 차이를 보이지 않았다. 앞으로 사람이나 가축으로 질병을 전파할 수 있는 진드기 매개질병 보균숙주로서 가능성이 있음으로 지속적인 조사 관찰이 필요하다.