• Title/Summary/Keyword: Blood chemical index

Search Result 32, Processing Time 0.027 seconds

An Effects of a New PGE1: Lipo-AS013 on Blood Flow and Survival of Skin Flap (새로운 PGE1인 Lipo-AS013이 피판의 혈류와 생존에 미치는 영향)

  • Seul, Chul Hwan;Choi, Jong Woo;Chi, Yong Hoon;Tark, Kwan Chul
    • Archives of Plastic Surgery
    • /
    • v.32 no.1
    • /
    • pp.5-11
    • /
    • 2005
  • Prostaglandin $E_1$($PGE_1$) is known to have various physiological action such as vasodilatation, decrease of blood pressure, angiogenesis, inhibition of platelet aggregation and so forth. $PGE_1$ has been developed in many different formulations in order to overcome its chemical instability and deactivation in the lungs when administered parenterally. Lipo-AS013 is a potent drug with higher chemical stability and greater vascular wall targeting than others. The study was done on $3{\times}10cm$ model flap of dorsal skin of Sprague-Dawley rats and the flap perfusion survival were observed and documented. The flap treated with Lipo-AS013 beforehand was given intravenously Sodium fluorescein 10 minutes later, and then Percent Dye Fluorescence Index(% DFI) was calculated. The results were compared to a control group and the group administered locally epinephrine.. In the control group, the % DFI and flap survival rate increased from $54.1{\pm}6.7$ to $65.0{\pm}2.6$(p<0.01) while in Lipo-AS013 group from $55.3{\pm}2.2$ to $67.4{\pm}1.9$(p<0.01), respectively. In the epinephrine group, the % DFI(p<0.05) and flap survival rate(p<0.001) decreased. In the both epinephrine and Lipo-AS013 group Percent DFI and flap survival rate are comparable with the control group.The result indicates that the potent Lipo-AS013 enhances the blood flow and flap survival. This highly potent Lipo-AS013 may have targeting ability and accumulate $PGE_1$ onto the vascular walls. A quantitative analysis of fluorescence on the skin surface is a reliable tool to measure the blood perfusion into an ischemic flap and its viability. Further comparative study with conventional $PGE_1$ and Lipo-$PGE_1$ is needed in order to clarify the action and efficiency of Lipo-AS013.

Four-week Repeated Dose Toxicity Test for Myelophil in SD Rats

  • Jung, Jong-Mi;Shin, Jang-Woo;Son, Jin-Young;Seong, Nak-Won;Seo, Dong-Seok;Cho, Jung-Hyo;Cho, Chong-Kwan;Son, Chang-Gue
    • The Journal of Korean Medicine
    • /
    • v.30 no.3
    • /
    • pp.79-85
    • /
    • 2009
  • Aim : To evaluate the pharmaceutical safety of the herbal formula Myelophil, composed of Astragali Radix and Salviae Radix, via systemic subacute toxicological study using SD rats. Methods : Forty male and 40 female SD rats were fed with Myelophil (5000, 2500 or 1250 mg/10 mL/kg) or distilled water for four weeks. Adverse effects were examined intensively by comparing the differences between normal and drug-administered groups using clinical signs, necropsies, histopathologic findings, hematology, urinalysis, and blood biochemical analysis. Results : No altered clinical symptoms including body weight, diarrhea, anorexia, death, and abnormal necropsy of major organs were observed in male or female rats. No drug-induced abnormalities in histopathological finding, hematological values, urinalysis, and blood biochemical values were found at any doses of Myelophil. Conclusion : Myelophil should be very safe when used in a clinical application with a wide therapeutic index.

  • PDF

Blood clot stabilization after different mechanical and chemical root treatments: a morphological evaluation using scanning electron microscopy

  • Stefanini, Martina;Ceraolo, Edoardo;Mazzitelli, Claudia;Maravic, Tatjana;Sangiorgi, Matteo;Zucchelli, Giovanni;Breschi, Lorenzo;Mazzoni, Annalisa
    • Journal of Periodontal and Implant Science
    • /
    • v.52 no.1
    • /
    • pp.54-64
    • /
    • 2022
  • Purpose: This in vitro study was conducted to evaluate the effects of different debridement techniques and conditioning procedures on root surface morphology and blood clot stabilization. Methods: Two debridement techniques (curette [CU] vs. high-speed ultrasound [US]) and 2 conditioning procedures (ethylenediaminetetraacetic acid [EDTA] and phosphoric acid [PA]) were used for the study. Seven experimental groups were tested on root surfaces: 1) no treatment (C); 2) CU; 3) US; 4) CU+EDTA; 5) US+EDTA; 6) CU+PA; and 7) US+PA. Three specimens per group were observed under scanning electron microscopy (SEM) for surface characterization. Additional root slices received a blood drop, and clot formation was graded according to the blood element adhesion index by a single operator. Data were statistically analyzed, using a threshold of P<0.05 for statistical significance. Results: The C group displayed the most irregular surface among the tested groups with the complete absence of blood traces. The highest frequency of blood component adhesion was shown in the CU+EDTA group (P<0.05), while no differences were detected between the CU, US+EDTA, and CU+PA groups (P<0.05), which performed better than the US and US+PA groups (P<0.05). Conclusions: In this SEM analysis, EDTA and conventional manual scaling were the most efficient procedures for enhancing smear layer removal, collagen fiber exposure, and clot stabilization on the root surface. This technique is imperative in periodontal healing and regenerative procedures.

Blood Biochemical Parameters, Physical Activity, Stress and Sleep Management by Body Mass Index (비만클리닉을 내원한 성인의 체질량지수에 따른 혈액생화학적 지표, 신체활동, 스트레스 및 수면관리)

  • Na, Yeon Kyung;Hong, Hae Sook;Suk, Hyun Jin
    • Journal of Korean Biological Nursing Science
    • /
    • v.16 no.2
    • /
    • pp.133-140
    • /
    • 2014
  • Purpose: The purpose of this study was to investigate the comparison of blood biochemical parameters, physical activity, stress, and sleep management between obese and non-obese subjects according to the body mass index (BMI). Methods: Data were collected from February 1 to May 31 in 2012. This research involved 403 subjects who visited an obesity clinic in Korea. They were divided into 4 groups: normal weight 100 ($18.5{\leq}BMI$ < $23.0kg/m^2$), overweight 100 ($23.0{\leq}BMI$ < $25.0kg/m^2$), obese 100 ($25.0{\leq}BMI$ < $30.0kg/m^2$), and severely obese 103 ($BMI{\geq}30.0kg/m^2$). Data were analyzed using SPSS version 19.0. Results: There were significant differences on the SGPT (F=22.98, p<.001), SGOT (F=12.91, p<.001), creatinine (F=4.82, p=.003), triglyceride (F=20.17, p<.001), and total cholesterol (F=6.55, p<.001) among the subjects according to the BMI. There was no significant association between frequency of physical activity according to the BMI. There was significant association regarding the management of sleep (F=5.13, p=.002), but no significant association regarding the management of stress. Overall, there were significant association regarding the management of stress and sleep (F=3.79, p=.011) among the groups. Conclusion: The result of this study suggests that it is possible to use the information as basic data for educational and nursing intervention programs based on the BMI.

Circumferential Alignment of Vascular Smooth Muscle Cells in a Cylindrical Microchannel

  • Choi, Jong Seob;Piao, Yunxian;Kim, Kyung Hoon;Seo, Tae Seok
    • Proceedings of the Korean Vacuum Society Conference
    • /
    • 2013.08a
    • /
    • pp.274.1-274.1
    • /
    • 2013
  • We report the circumferential alignment of human aortic smooth muscle cells (HASMCs) in an orthogonally micropatterned circular microfluidic channel to form an in vivo-like smooth muscle cell layer. To realize a biomimetic smooth muscle cell layer which is aligned perpendicular to the axis of blood vessel, we first fabricated a half-circular polydimethylsiloxane (PDMS) microchannel by soft lithography using a convex PDMS mold. The orthogonally micro wrinkle patterns were generated inside the half-circular microchannel by stretching-releasing operation under UV irradiation. Upon UV treatment with uniaxial 40 % stretch of a PDMS substrate and releasing process, the microwrinkle patterns perpendicular to the axial direction of the circular microchannel were generated, which could guide the circumferential alignment of HASMCs successfully during cultivation. The analysis of orientation angle, shape index, and contractile protein marker expression indicates that the cultured HASMCs revealed the in vivo-like cell phenotype. Finally, we produced circular microchannels by bonding two half-circular microchannels, and cultured the HASMCs circumferentially with high alignment and viability for 5 days. These results are the first demonstration for constructing an in vivo-like 3D smooth muscle cell layer in the circular microfluidic channel which can provide novel bioassay platforms for in-depth study of HASMC biology and vascular function.

  • PDF

Extraction Condition from Schizandra chinensis Baillon for Beverage Develooment of High Scizandrin Concentration (Schizandrin함량이 높은 음료개발올 위한 오미자 (Schizandra chinensis Baillon) 추출조건)

  • Choi, Eun-Oh;Lee, Bong-Soo;Park, Yong-Su;Seo, Eun-Ok;Chung, Bong-Woo
    • KSBB Journal
    • /
    • v.23 no.3
    • /
    • pp.271-275
    • /
    • 2008
  • Omija (Schizandra chinensis Baillon) has five lignans such as schizandrin, gomisin A, gomisin N, gomisin C and schizandrin C. Its major constituent, schizandrin, has a deterrent effect on nephrotoxicity of cisplatin, blood sugar and an ulcer of the stomach. In this study, we have investigated optimized extraction condition to make high functional beverage by quantifying schizandrin and measuring of browning index. Browning index was used as an index of color deterioration of anthocyanin. Browning index was decreased according to increment in extraction temperature and ethanol concentration. The optimum extraction time, extraction temperature and alcohol concentration levels were 3 hrs, $46^{\circ}C$ and 24% alcohol for high schizandrin concentration and desired browing index.

Self-care Behaviors And Physiological Index According to Cognitive Function of Hemodialysis Patients (혈액투석환자의 인지기능에 따른 자가간호 행위와 생리적 지표)

  • Jang, Yeon-Hwa;Oh, Hyo-Sook;Jang, Geum-Song
    • The Korean Journal of Health Service Management
    • /
    • v.6 no.2
    • /
    • pp.57-69
    • /
    • 2012
  • The purpose of this study was to investigate correlation with self-care behaviors and physiological index according to cognitive function of hemodialysis patients. The data were collected from 108 patients on hemodialysis in university hospital. The period of data collection was from January 4 to January 31, 2010. The instruments of research were MMSE, Self-care behavior scale for hemodialysis patients. The 17.6% of patients showed the cognitive disorder. Self-care behaviors of patients showed that normal cognitive function group was significantly higher than dysfunction group. Creatinine and parathyroid hormone of patients showed that normal cognitive function group was significantly lower than dysfunction group. The cognitive function of patients showed positive correlations with self-care behavior(r=.283, p=.003) and Kt/V(r=.214. p=.026), showed significant negative correlations with phosphorus(r=-.223, p=.020) and creatinine(r=-.318, p=.001). The results of this study can be used to develop nursing education program to improve self-care behaviors according to cognitive function of hemodialysis patients.

Effect of Particle Size of Forage in the Dairy Ration on Feed Intake, Production Parameters and Quantification of Manure Index

  • Moharrery, A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.23 no.4
    • /
    • pp.483-490
    • /
    • 2010
  • The objectives of this study were to measure particle size and evaluate the effect of increasing alfalfa hay particle size on production characteristics in lactating Holstein dairy cows. Ninety multiparous Holstein cows in early to mid-lactation were randomly assigned in a complete randomized design for a 30-day period. Animals were offered one of the three diets, which were identical in energy, protein, and chemical composition, but differed only in particle size of alfalfa hay. The treatments were A) total mixed ration (TMR) in which only fine chopped alfalfa hay was incorporated in the ration, B) the same diet in which half of the alfalfa hay was fine chopped and incorporated in the mixed ration and half was long hay and offered as a top dressing, and C) the same diet with long hay alfalfa offered as a top dressing. Distribution of particle size of rations was determined through 20,000, 8,000 and 1,000 ${\mu}m$ sieves. The new method of quantitative determination of manure index was examined for each cow on different treatments. The geometric mean length of particle size in the rations was 5,666, 9,900 and 11,549 ${\mu}m$ for treatments A, B and C, respectively. Fat corrected milk (4%), milk fat percentage and production were significantly different (p<0.05) in treatment A versus B and C (fat corrected milk (FCM, 4%)) 28.3 vs. 35.2 and 32.3 kg/d, fat percentage 2.89, 4.04 and 3.62; but the change of ration particle size had no significant effect on milk production (p>0.05). Blood concentration of cholesterol in treatment A was significantly higher (p<0.05) than treatment B and C (181.0 vs. 150.0 and 155.2 mg/dl). Manure index in treatment C was significantly different (p<0.05) from treatment B (15.86 vs. 17.67). Based on these experimental findings, it is concluded that an increase in the ration particle size can increase milk fat percentage due to providing more physically effective fiber, which in turn could effect changes in manure consistency.

Characterization of Binding Mode for Human Coagulation Factor XI (FXI) Inhibitors

  • Cho, Jae Eun;Kim, Jun Tae;Jung, Seo Hee;Kang, Nam Sook
    • Bulletin of the Korean Chemical Society
    • /
    • v.34 no.4
    • /
    • pp.1212-1220
    • /
    • 2013
  • The human coagulation factor XI (FXI) is a serine protease that plays a significant role in blocking of the blood coagulation cascade as an attractive antithrombotic target. Selective inhibition of FXIa (an activated form of factor XI) disrupts the intrinsic coagulation pathway without affecting the extrinsic pathway or other coagulation factors such as FXa, FIIa, FVIIa. Furthermore, targeting the FXIa might significantly reduce the bleeding side effects and improve the safety index. This paper reports on a docking-based three dimensional quantitative structure activity relationship (3D-QSAR) study of the potent FXIa inhibitors, the chloro-phenyl tetrazole scaffold series, using comparative molecular field analysis (CoMFA) and comparative molecular similarity analysis (CoMSIA) methods. Due to the characterization of FXIa binding site, we classified the alignment of the known FXIa inhibitors into two groups according to the docked pose: S1-S2-S4 and S1-S1'-S2'. Consequently, highly predictive 3D-QSAR models of our result will provide insight for designing new potent FXIa inhibitors.

Effect of Green Coffee Bean Extract Supplementation on Body Fat Reduction in Mildly Obese Women (경도비만 여성에서 생커피두 엑기스의 섭취에 의한 체지방 감소 효과)

  • Kim, Tae-Su;Yang, Woong-Suk;Park, So-I;Lee, Sung-Pyo;Kang, Myung-Hwa;Lee, Jae-Hwan;Park, Il-Bum;Park, Hyun-Jun;Murai, Hiromichi;Okada, Tadashi
    • Journal of the Korean Society of Food Culture
    • /
    • v.27 no.4
    • /
    • pp.407-413
    • /
    • 2012
  • In previous studies, we performed joint animal studies and clinical trials between Yonsei University and Oryza Oil & Fat Chemical Co. Ltd. We have shown that coffee bean extract has potent anti-obesity and hypotriglyceridemic activities as well as beneficial effects on body fat reduction.In this study, the effects of coffee bean extract (100 mg/capsule) on body fat reduction were evaluated in overweight/obese women (body mass index of 25~30 $kg/m^2$ or body fat > 30%) not diagnosed with any type of disease. Subjects were randomly assigned to a coffee bean extract group (n=10) or placebo group (n=10). We measured anthropometric parameters, abdominal fat distribution by computed tomography and blood components before and after the 8week intervention period. After supplementation, the coffee bean extract group showed body weight (p=0.08), body mass index (p=0.06), hip circumference (p<0.05), and upper waist circumference (p< 0.01). In addition, after 8 weeks, the coffee bean extract group showed a significant decrease in abdominal internal fat area compared to 0 weeks (0 weeks : $155.8cm^2$; 8 weeks : $145.9cm^2$, ${\Delta}$ change : $-9.9cm^2$, respectively). However, there were no significant differences in lipid profiles or serological measurements between the coffee bean extract group and placebo group. The results of our human study demonstrated that coffee bean extract supplementation for 8 weeks has beneficial effects on reducing abdominal internal fat area as well as hip and waist circumferences.