• Natural Product Sciences
• /
• 제17권4호
• /
• pp.291-295
• /
• 2011

The present investigation evaluated the safety of the methanol extract from the fruit body of Phellinus pini Ames (PPA) by determining its potential toxicity after a subacute administration in rats. The extract was orally administered in doses of 1 g/kg, 2 g/kg, and 4 g/kg daily for 14 days to rats. Body weight, biochemical, and hematological parameters were determined at the end of 14 days of daily administration. The no-observed adverse effect levels (NOAEL) of the extract were 4 g/kg, when given by gavage routes. Daily oral administration of PPA extract for up to 14 days did not result in the death of significant changes in the body weight, hematological, and mainly biological parameters. In biological analysis, some significant changes occurred, including triglyceride and blood urea nitrogen (BUN), indicating that the PPA extract has liver and kidney-modulating activity. The PPA extract was found to be low or non-toxic in rats.

• Biomolecules & Therapeutics
• /
• 제10권2호
• /
• pp.89-98
• /
• 2002

In this study general pharmacological profiles of KI-60606 on the central nervous system, the cardiovascular system and the other organs were investigated. The dosages given were 0,5, 10 and 25 mg/kg and drugs were administered intravenously. The animals used for this study were mice, rats, cats and guinea pigs. KI-60606 showed no effects on general behavior, motor coordination, spontaneous locomotor activity, hexobarbital-induced hypnosis time, body temperature, analgesic activity, anticonvulsant activity and contraction of nictitating membrane in cats. Furthermore KI-60606 showed no effects on blood pressure, heart rate, LVP (left ventricular peak systolic pressure), LVEDP (left ventricular end diastolic pressure), LVDP (left ventricular developing pressure), DP(double product), CFR(coronary flow rate), smooth muscle contraction using guinea pig ileum and gastric secretion at all dosage tested except the increase of gastrointestinal transport and urinary $K^+$ excretion.

• Journal of Microbiology
• /
• 제39권3호
• /
• pp.181-185
• /
• 2001

The morphological characteristics of newly isolated Cordyceps hepialidicola were characterized, and the phylogenetic relationships with other Cordyceps species were investigated using a sequence analysis of the internal transcribed spacer (ITS). The PCR product of 592 bp showed a homology of 92 and 91% with C. militaris and C. nutans, respectively, In an in vitro model using mouse peripheral blood mononuclear cells (PBMC), a methanol extract of C. hepialidicola induced multiple cytokines, including IFN-${\gamma}$ IL-4, and IL-18. The extract also enhanced the percentages of the CD4$\^$+/ and CD8$\sub$+/ T cells in the healthy murine PBMCs to 56.1% and 13.0%,respectively. The percentages of CD4$\^$+/ and CD8$\^$+/ in the untreated controls were 28.4 and 7.3%, and concanavalin A-treated positive controls were 62.4 and 18.3%, respectively.

• Natural Product Sciences
• /
• 제12권3호
• /
• pp.153-156
• /
• 2006

The antisnake venom activity of Clerodendrum viscosum Vent. (Fam. Verbenaceae), a plant traditionally used in India for the treatment of snake bite was evaluated by in vitro and in vivo methods. While in vitro studies were performed using human blood, in vivo studies were carried out using mice administered three different i.p doses of the extract, 5 min before the administration of Naja naja snake venom. The results of the in vitro studies showed that the extract probably interacts with but does not stabilize membrane protein. In the in vivo studies the extract showed significant antisnake venom activity, which may be attributed to its possible interference with the acetylcholine receptor sites. Hence the present investigation justifies the traditional use of Clerodendrum viscosum (C. viscosum) as antisnake venom.

• Natural Product Sciences
• /
• 제25권4호
• /
• pp.293-297
• /
• 2019

Policosanol extracted from sugarcane wax is a generic term used for total fatty alcohols obtained from esterification of fatty acids. It has been approved as a health functional food by the Ministry of Food and Drug Safety of Korea in 2006. Policosanol is well-known to aid in lowering blood cholesterol level. Recently, several studies have reported the physiological activities of policosanol, such as anti-inflammatory effects, antioxidant effects, and lowering of the incidence of ageing-related diseases, for example, hypertension, stroke, among others. This review describes the physiological activities of policosanol and its applications in the field of health functional foods.

• Natural Product Sciences
• /
• 제5권4호
• /
• pp.155-158
• /
• 1999

Several biological activities of extracts from roots of Cichorium intybus Linne (Compositae) were studied in this paper. The antiinflammatory activity of the methanol extract of this root was investigated against carrageenin induced edema in rat‘s hind paw. Significant inhibitory effects were observed at the dose of 1,000 mg/kg and were compared with aspirin as a control. The hepatoprotective activities of the methanol extract, ethylacetate and butanol fraction were studied on mice whose livers are damaged by $CCl_4$. The serum transaminase activities (ALT, AST) were reduced at the dose of 1,000 mg/kg of the methanol extract, 500 mg/kg of ethylacetate and butanol fraction, respectively. The bile juice secretion was also increased significantly from each fraction. The antidiabetic activity was examined on strepto-zotocin-induced diabetic rats with methanol extract. Methanol extract gave a significant reduction of blood glucose levels in 1 week and 3 weeks.

• Natural Product Sciences
• /
• 제7권4호
• /
• pp.107-109
• /
• 2001

To investigate anti-diabetic component from the node of lotus rhizome (Nelumbo nucifera; Nymphaeaceae), activity guided isolation was conducted. One amino acid was isolated from active fraction of the aqueous methanolic extract. The structure of this compound was identified as tryptophan (1) by the analysis of spectroscopic evidences and comparisons with the data of authentic samples. Tryptophan reduced the blood glucose level significantly in glucose-fed hyperglycemic mice compared with glucose-treated group and exhibited 44.3% of activity compared with tolbutamide,.

• Natural Product Sciences
• /
• 제23권4호
• /
• pp.286-290
• /
• 2017

Multidrug-resistant Acinetobacter baumannii and Pseudomonas aeruginosa are highly dangerous nosocomial pathogens, cause the symptoms of skin infections, pressure sores, sepsis, blood stream and wound infections. Unfortunately, these pathogens are immune to the most common antibiotics, such as, carbapenem, aminoglycoside and fluoroquinolone. Therefore, it is imperative that new and effective antibiotics be developed. In the present study, the antimicrobial effects of Aloe vera MAP (modified Aloe polysaccharide) on Staphylococcus aureus and Bacillus subtilis, Escherichia coli and Enterobacter aerogenes, and clinical Pseudomonas aeruginosa and clinical Acinetobacter baumannii were comprehensibly investigated. Prior to the growth inhibition effect measurement and antibiotic disc diffusion assay on gram-positive and gram-negative bacteria and selected multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii, antimicrobial resistance screening was performed for the multidrug-resistant bacteria obtained from clinical isolates. The results for showed the Aloe vera MAP had a concentration-dependent effect on all of examined bacteria, particularly on Pseudomonas aeruginosa. Anti-inflammatory and anti-oxidant experiments were also performed dose dependently effects to confirm the beneficial physiological effects of Aloe vera MAP.

• 한국식생활문화학회지
• /
• 제9권5호
• /
• pp.489-497
• /
• 1995

The purpose of this study was to survey the various kinds of cooked beef products focusing on 'Chim' (a steamed beef products) through historical literatures written from 1670 to 1945 in korea. 'Chim' was recorded 25 times in the references and could be classified to 5 groups based on major ingredients such as stomach, intestine, lean meat, tail and ribs. Among the eleven cooked products of 'Chim', 'Kalbi-chim' (a steamed ribs) was recorded most frequently and 'Sundae-chim' (sausage-like product) was the next. Though 'Kalbi-chim' has been used untill present day but 'Sundae-chim' was disappeared in the early 19th centuries. Ribs were most popular ingredient among the major ingredients such as stomach, intestines, lean meat, tail, feet and ribs. Twenty one kinds of subingredients including pheasant meat, blood clot and flour were used for 'Chim'. Decorating ingredients such as roasted sesame power, pine nut and thin-layer fried eggs and seasonings such as pepper, roasted sesame powder, tripe, garlic, soy sauce and seasonings such as pepper, roasted sesame powder, tripe, garlic, soy sauce and sesame oil were used for 'Chim'.

• KSBB Journal
• /
• 제15권5호
• /
• pp.423-427
• /
• 2000

바이오센서의 구조와 기능 그리고 종류에 대해 소개하였다. 이 분야의 연구가 과거 15년간 각국에서 경쟁적으로 연 구하였으나 glucose sensor가 상품화되 었을 뿐 연구결과를 상 품화한 것은 극히 적은 수였다. 그러나 최근 몇 년간 특히 포켓용 i-STAT point-of-care system의 도입 그리고 surface plasmon resonance와 evanescent wave 측정 장치 의 출현으로 상품화된 바이오샌서의 수가 크게 증가하는 추세에 었다. 이들 중 의료임상과 생물공정 그리고 환경오염 측정용으로 응 용되고 있는 몇 가지 상품에 대하여 약술하였다.