• Journal of Periodontal and Implant Science
• /
• 제36권4호
• /
• pp.913-923
• /
• 2006

목적 : 최근 발치 후 즉시 임플란트 식립은 널리 사용되는 수술 방식이다. 이 연구의 목적은 임플란트 주위 골결손시 Ca-P으로 표면 처리된 이종골을 사용하여 골재생을 평가하기 위함이다. 재료와 방법 : 두 마리의 개 모델에서 하악 소구치와 제일 대구치를 발치하였다. 발치 6주 후 trephine bur를 이용하여 7.5 mm 지름과 5 mm 깊이를 가진 결손부를 형성하였다. 이 후 이 결손부의 중앙에 3.5 mm 지름과 15mm 길이의 fixture(GS II)를 식립하였다. 결과적으로 임플란트와 주변을 둘러싸고 있는 골 사이에는 2.0 mm정도의 gap이 만들어진다. 준비된 결손부 내로 자기골 또는 $Biocera^{(R)}$를 채웠다. 각각 4주, 8주 후 조직 절편을 제작하였다. 조직학적 평가를 위해 Block biopsy를 시행하였다. 결과 : 두 집단 모두 임상적으로 골이 완전히 채워졌다. 자가골이 이식된 부위(control)의 평균 골-임플란트 접촉(BIC)은 각각 4주째 $28.2{\pm}19%$였고, 8주째 $44.9{\pm}9%$였다. $Biocera^{(R)}$가 이식된 부위(test)의 평균 BIC는 각각 4주째 $34.6{\pm}27%$였고, 8주째 $27.6{\pm}23%$였다. 자가골이 이식된 부위(control)의 평균 골밀도는 각각 4주째 $32.7{\pm}25%$, 8주째 $37.4{\pm}17%$였다. 골-임플란트 접촉(BIC)과 골밀도의 평균 비율(%)은 비슷하였다. 조직학적으로 자가골과 이종골 이식 부위 모두 주변골과 잘 조화를 이루었고 유사한 치유 양상이 관찰되었다. 자가골과 이종골 이식 부위간 유의한 차이는 없었다.(P>0.05) 결론 : 임플란트 주위 2 mm의 골 결손부위에 자가골 또는 이종골로 채운 경우 유사한 결과를 얻었다. 이 결과 임플란트 fixture 주위의 골 결손부 해소를 위해 자가골을 대체할 수 있는 재료로 $Biocera^{(R)}$를 사용할 수 있음을 보여준다.

• Journal of Periodontal and Implant Science
• /
• 제38권1호
• /
• pp.51-58
• /
• 2008

Purpose: In dental clinical fields, various periodontal membranes are currently used for periodontal regeneration. The periodontal membranes are categorized into two basic types: resorbable and non-resorbable. According to the case, clinician select which membrane is used. Comparing different membranes that are generally used in clinic is meaningful. For this purpose, this study evaluates histological effects of various membranes in canine one wall intrabony defect models and it suggest a valuation basis about study model. Material and Method: The membranes were non-resorbable TefGen $Plus^{(R)}$, resorbable Gore Resolut $XT^{(R)}$ and resorbable $Osteoguide^{(R)}$. One wall intrabony defects were surgically created at the second and the mesial aspect of the fourth mandibular premolars in either right or left jaw quadrants in two dogs. The animals were euthanized 8 weeks post-surgery when block sections of the defect sites were collected and prepared for histological evaluation. Results: 1. While infiltration of inflammatory cells were observed in control, TefGen $Plus^{(R)}$ and Gore Resolut $XT^{(R)}$, it was not observed in $Osteoguide^{(R)}$. 2. TefGen $Plus^{(R)}$ had higher integrity than others and $Osteoguide^{(R)}$ was absorbed with folding shape. Gore Resolut $XT^{(R)}$ was divided everal parts during resorbtion and it was also absorbed from inside. 3. Quantity of new bone and new cementum was not abundant in all membranes. 4. For histologic evaluation of membranes we should consider infiltration of inflammatory, migration of junctional epithelium, integrity of membrane, quantity of new bone and new cementum, connective tissue formation and aspect of resorption. Conclusion: This histologic evaluation suggests that $Osteoguide^{(R)}$ provides periodontal regenerative environment with less inflammatory state. It is meangful that this study model suggests a valuation basis about other study model.

• Journal of Acupuncture Research
• /
• 제22권2호
• /
• pp.71-81
• /
• 2005

Objective : Dear antler (Cervus korean TEMMINCK var. mantchuricus Swinhoe) used for traditional immunosuppressive and immuno-activating action. The effect of deer antler herbal-acupuncture(DAH) solution, prepared by water extract method, on cathepsin activities in bone tissues (cartilage and synovial) cells from mouse rheumatoid arthritis (RA) model was studied. The cysteine endoprotease cathepsin mediates degradation of the MHC class II invariant chain (Ii) in human and mouse antigen-presenting cells. The studies described here examine the functional significance of cathepsin inhibition on autoantigen presentation and organ-specific autoimmune diseases in a murine model for RA. Methods : An animal model for RA in BALB/c mice thymectomized 3 days after birth (3d-Tx) was constructed All 3d-Tx BALB/c mice developed autoimmune lesions in the bone tissue cells, starting at 3 weeks of age, and the disease mediated by CD4+ T cells was chronic and progressive. Significant inhibitory effects of DAH solution on cathepsin S and L were observed in each organ in a dose-dependent manner. Moreover, we confirmed that cathepsin S and L activity in each organ were clearly inhibited by DAB solution. When we examined the inhibitory effects of DAH solution against autoantigen-specific T cell responses in vitro, in regional lymph node cells, but not in spleens, from model mice, a significant inhibitory effect of DAB solution was observed in a dose-dependent manner. DAH solution do not block T cell proliferation to Con A, indicated that the dose of DAB solution 10 to $20\;{\mu}g/m{\ell}$ was sufficient to inactivate the autoantigen-specific T cell responses in vitro. In vivo therapeutic effects of DAB solution were examined in a murine model for RA, autoantigen-specific (C-II-specific) T cell response were significantly inhibited in LNCs from DAH solution-treated mice. Results : Iinhibition of cathepsin S and L in vivo alters autoantigen presentation and development of organ-specific autoimmunity in RA model. Conclusion : These data identify selective inhibition of cysteine protease cathepsin S and L as a potential therapeutic strategy for autoimmune disease process such RA. Thus, DAH solution will served as a potent anti-inflammatory and anti-arthritic agents for treatment of human RA.

• 대한치과교정학회지
• /
• 제25권4호
• /
• pp.403-414
• /
• 1995

본 연구는 영구치열이 완성된 원숭이 2마리를 실험 동물로 선정하여 전치간 수직 고무와 연속 호선에 의한 개교 치료 기전으로 수직피개도가 증가하였을 때, 하악 치주 조직의 조직학적 변화를 고찰하였다. 상악에 구치부 교합거상판을 장착시켜 일시적인 전치부 개교 상태를 유발하면서 상악을 고정원으로 안정화하였고, 하악에는 양측 제2대구치를 발거한 후 나머지 모든 치아에 주조금관을 제작하였다. 각 주조금관에 018inch 표준 브라켓을 납착하여 구내 고정하고, 대조동물에는 $016{\times}022$inch의 ideal archwire를, 실험동물에는 같은 크기의 MEAW(multiloop edgewise archwire)를 삽입하였다. 2주 동안 전치간 수직 고무를 적용하고 희생하였으며, 하악 치주 조직 표본을 관찰한 결과, 다음과 같은 결론을 얻었다. 1. 2주간의 실험 기간 동안 수직피개도는 대조동물에서 0.3mm, 실험동물에서 1.3mm 증가되었다. 2. 대조동물과 실험동물 모두 중절치로부터 제1소구치까지는 정출력을 받고, 제2소구치와 제1대구치는 압하력을 받은 것으로 나타났다. 3. 실험동물의 정출된 하악 절치부 치주인대는 대조군에 비하여 견인 방향에 따른 섬유 구조의 재배열이 두드러지고, 치근단과 치조정부 및 치근 측면 치조골에서 신생골 형성이 현저하였다. 4. 실험동물의 정출된 하악 절치부 치주인대의 견인력이 분포한 영역에서 과도한 치근 흡수나 초자양 변성은 관찰되지 않았다. 5. 전치부와 구치부의 치조골면에서의 골개조 현상이 대조동물에 비해서 실험동물에서 더 뚜렷하였다.

• 한국가금학회지
• /
• 제17권4호
• /
• pp.296-302
• /
• 1990

Aflatoxin $B_1$($AFB_1$ 0 또는 1ppm)과 Vitamin $D_3$($VD_3$0, 500, 1,000 또는 1,500IU/kg)의 교호작용을 조사코자, Hubbard계통 broiler 병아리 336수를 이용, 2$\times$4 요인시험이 실시되었으며, 전체8처리가 3반복으로 설계되어 탈지건조 중족골의 회분 및 무기물함량과 중량 등을 측정하였다. 얻어진 결과를 요약하면 다음과 같다. L 중족골의 신선중량에 있어서 $AFB_1$ 급여에 의한 차이는 나타내지 않았으며 $VD_3$의 수준이 증가됨에 따라 감소되는 경향을 보였다(P＜.01). 2. 탈지건조 중족골의 회분함양을 보면 $AFB_1$의 급여로 인하여 감소되었으며(P＜.01), $VD_3$수준이 증가될 때 반대로 증가되었다(P＜.01). 교호효과는 인정되지 않았으나 $AFB_1$ 1ppm 급여로 인한 회분감소를 극복하기 위하여 $VD_3$ 1,500IU/kg의 급여가 필요하다는 것이 증명되었다. 3. Ca의 함양은 AFB$_1$급여의 영향을 받지 않았으나 $VD_3$에 의하여 증가되는 경향을 보였다(P＜0.05). 4. P의 함양은 $AFB_1$급여로 감소되었으나(P＜.01) $VD_3$의 영향은 없었다. 5. Cu의 함양은 AFB$_1$$AFB_1$에 의하여 감소되었다(P＜.05). 6. Na Mg, K, Zn, Fe 및 Mn의 함량은 $AFB_1$이나 $VD_3$에 의하여 영향을 받지 않았다.

• Journal of Periodontal and Implant Science
• /
• 제23권3호
• /
• pp.577-594
• /
• 1993

Polytetrafluoroethylene membrane 및 millipore filter를 섬유소와 병용사용하여 성견 치주조직 재생에 미치는 영향에 관해 알아보기 위하여 생후 9개월 이상된 잡종성견7마리에 치은박리소파수술후 PTFE membrane를 삽입한 군을 대조군으로, PTFE membrane에 섬유소를 첨가했는 군을 실험1군으로, 수술시 millipore filter를 봉합에 의해 고정시킨군을 실험2군으로, 섬유소로 millipore filter를 고정시킨 군을 실험3군으로 하였다. 술후 각 1, 2, 4, 8, 12 주에 관류고정과 아울러 희생시켜 악골을 채취하고 통법에 의해 후고정, 탈회, 탈수과정을 거쳐 파라핀으로 포매한 후 $4-6{\mu}m$두께의 협, 설측 박편을 제작한 후 H & E 및 Masson’s trichrome염색 후 광학현미경으로 치유결과를 조직학적으로 비교 관찰하여 다음과 같은 결과를 얻었다. 1 주때 대조군 및 실험각군 모두 치은상피조직의 근단이동이 억제된 양상을 나타내었으며, millipore filter를 삽입한 군에서는 섬유소보다 봉합에 의해 막을 고정시킨 경우 술후 1주때 부터 치은퇴축으로 인한 막의 노출이 관찰되었고 술후 4주때 막의 내, 외면에 긴접합상피조직의 근단이동을 관찰할 수 있었으며, PTFE membrane 경우는 섬유소유무에 따른 차이는 관찰되지 않았다. 대조군 및 실험각군 모두 술후 1주때 골양조직을 관찰할 수 있었고 4주때 성숙된 치조골 양상을 보였으며 특히 PTFE membrane인 경우 증가된 협설 측 폭경을 관찰할 수 있었으며, 술후 12주때 실험각군 모두 치조골이 더욱 성숙된 양상을 관찰할 수 있었다. 술후 4주때 대조군, 실험각군 모두 활택된 치근면상에 미약한 신생백악질 및 불규칙적인 교원섬유의 배열을 관찰할 수 있었고 8주때 뚜렷한 백악질의 증가와 12주때에는 신생백악질과 신생치조골 사이에 기능적으로 배열된 교원 섬유를 관찰할 수 있었다. 결론적으로, 섬유소로 막을 고정시킬 경우 치근면과의 긴밀한 접촉을 유지시킬수 있으며, 차단막을 근단쪽으로 위치시켜 막의 노출 위험을 감소시킬수 있으며, 특히 PTFE membrane인 경우 봉합대체물로의 연이은 연구가 필요할 것으로 사료된다.

• Journal of Periodontal and Implant Science
• /
• 제36권2호
• /
• pp.555-565
• /
• 2006

The purpose of the present study is to evaluate the biological stability of the zirconia/alumina composite abutment by histologic and radiographic examination in clinical cases. 17 partially edentulous patients (5 men and 12 women, mean age 47) were treated with 37 implants. The implants were placed following the standard two-stage protocol. After a healing period of 3 to 6 months, zirconia/alumina composite abutments were connected. All radiographs were taken using paralleling technique with individually fabricated impression bite block, following insertion of the prosthesis and at the 3-, 6-, 12 month re-examinations. After processing the obtained images, the osseous level was calculated using the digital image in the mesial and distal aspect in each implant. An ANOVA and t-test were used to test for difference between the baseline and 3-, 6-, 12 months re-examinations, and for difference between maxilla and mandible. Differences at P <0.05 were considered statistically significant. For histologic examination, sample was obtained from the palatal gingiva which implant functioned for 12 months. Sections were examined under a light microscope under various magnifications. Clinically, no abutment fracture or crack as well as periimplantitis was observed during the period of study. The mean bone level reduction(${\pm}standard$ deviation) was 0.34 rom(${\pm}\;0.26$) at 3-months, 0.4 2mm(${\pm}\;0.30$) at 6-months, 0.62 mm(${\pm}\;0.28$) at 12-months respectively. No statistically significant difference was found between baseline and 3-, 6-, 12-months re-examinations (p > 0.05). The mean bone level reduction in maxilla was 0.33(${\pm}0.25$) at 3-months, 0.36(${\pm}0.33$) at 6-months, 0.56(${\pm}0.26$) at 12-months. And the mean bone level reduction in mandible was 0.35(${\pm}0.27$) at 3-months, 0,49(${\pm}0.27$) at 6-months, 0.68(${\pm}0.30$) at 12-months. No statistical difference in bone level reduction between implants placed in the maxilla and mandible. Histologically, the height of the junctional epithelium was about 2.09 mm. And the width was about 0.51 mm. Scattered fibroblasts and inflammatory cells, and dense collagen network with few vascular structures characterized the portion of connective tissue. The inflammatory cell infiltration was observed just beneath the apical end of junctional epithelium and the area of direct in contact with zirconia/alumina abutment. These results suggest the zirconia/alumina composite abutment can be used in variable intraoral condition, in posterior segment as well as anterior segment without adverse effects.

• Asian-Australasian Journal of Animal Sciences
• /
• 제9권3호
• /
• pp.271-279
• /
• 1996

On-farm experiments were carried out in dryland farming in Bali for 48 weeks to study the effect of flock size on the growth and carcass characteristics of cross-bred goat fed gliricidia-supplemented diet. Eighty four bucks with average live weight of 15.87 kg were allocated in a completely randomized block design arrangement, consisted of three treatments and four blocks. The treatments were $3goats/2.7m^2$ (A), $6goats/5.4m^2$ (B) and $12goats/10.8m^2$ (C), while the floor density was the same ($0.9m^2$ per goat). Feed consumed by goat B was similar (p > 0.10), while feed consumed by goat C was lower (p < 0.10) than goat A. Live weight gain of goat B and C were lower (p<0.05) than goat A. FCR of goat B was higher (p < 0.10) than goat A, while FCR of goat C was similar (p > 0.10) with goat A. Goat B has heavier (p < 0.10) head and digestive tract, while goat C has heavier (p<0.10) hindlegs and digestive tract than goat A. Goat B has lighter (p < 0.10) shoulder, while goat C has lighter shoulder and heavier legs (p < 0.10) than goat A. The carcass quality (measured in terms of loin eye muscle area, meat, bone and fat portions) were not affected (p > 0.05) by the flock sizes.

• Asian-Australasian Journal of Animal Sciences
• /
• 제9권4호
• /
• pp.381-387
• /
• 1996

An experiment was carried out for 12 weeks to study the feeding behaviour, growth and carcass characteristics of cross-bred goats. Nine bucks with an average live weight of $18.02{\pm}0.9kg$ were allocated in a completely randomized block design arrangement, consisting of three feeding regimes and three blocks. The feeding regimes consisted of 100% natural grass (Treatment A), 100% gliricidia leaf (Treatment B) and 100% ficus leaf (Treatment C). Frequency of turning-over and sniffing the feed and duration and frequency of ruminating, defaecating, and urination of goats in treatment B were longer, while duration of masticating and jaw movement of chewing one bolus was shorter than those of goats in treatments C and A (p < 0.05). Furthermore, goats in treatment B gained more live weight, consumed more feed, consumed less water and was more efficient in utilizing the feed than goats in treatments C or A (p < 0.05). The dressing percentage, carcass length and carcass fat of goats in treatment B were higher and their bone percentages were lower than those in treatments C and A (p < 0.05). There is an indication that feeding gliricidia and ficus leaves during the dry season could not only increase the body weight and improve carcass quality of the goat but also save water for household needs.

• Journal of Korean Neurosurgical Society
• /
• 제49권6호
• /
• pp.351-354
• /
• 2011

Objective : This investigation was conducted to evaluate a new, safe entry point for the C2 pedicle screw, determined using the anatomical landmarks of the C2 lateral mass, the lamina, and the isthmus of the pars interarticularis. Methods : Fifteen patients underwent bilateral C1 lateral mass-C2 pedicle screw fixation, combined with posterior wiring. The C2 pedicle screw was inserted at the entry point determined using the following method : 4 mm lateral to and 4 mm inferior to the transitional point (from the superior end line of the lamina to the isthmus of the pars interarticularis). After a small hole was made with a high-speed drill, the taper was inserted with a 30 degree convergence in the cephalad direction. Other surgical procedures were performed according to Harm's description. Preoperatively, careful evaluation was performed with a cervical X-ray for C1-C2 alignment, magnetic resonance imaging for spinal cord and ligamentous structures, and a contrast-enhanced 3-dimensional computed tomogram (3-D CT) for bony anatomy and the course of the vertebral artery. A 3-D CT was checked postoperatively to evaluate screw placement Results : Bone fusion was achieved in all 15 patients (100%) without screw violation into the spinal canal, vertebral artery injury, or hardware failure. Occipital neuralgia developed in one patient, but this subsided after a C2 ganglion block. Conclusion : C2 transpedicular screw fixation can be easily and safely performed using the entry point of the present study. However, careful preoperative radiographic evaluation, regardless of methods, is mandatory.