• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.257-266
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• 2006

Studies were carried out to evaluate the effect of liquid pig manure on the production of Japanese millet, the chemical characteristics of pasture soil and leaching water. The study was undertaken from June to September 2005. Randomized complete block design was used to allot four treatments: T1 (no fertilizer), T2 (N : 200 kg/ha, p : 150 kg/ha, K : 150 kg/ha), T3 (liquid pig manure containing 1.7% DM 200 kg N/ha) and T4 (liquid pig manure containing 7.0% DM 200 kg N/ha). Leaching water was sampled at 21 August (1st time), 9 September (2nd time) and 26 September (3rd time), 2005, respectively. No significant differences in the dry matter yield of Japanese millet was found among the four treatments, whereas the plant lengths of Japanese millet were higher in the T2, T3 and T4 than in T1 (p<0.05). Nitrogen, P and K uptake of J. millet tended to be influenced by application of chemical fertilizer or 7.0% DM liquid pig manure compared with T1 or 1.8% DM liquid pig manure. The organic matter (OM) content of soil was higher in T2, T3 and T4 than in T1. Na content was highest in T3 among the four treatments. $NO_{3^-}N\;or\;NH_{4^-}N$ content in leaching was not different among the four treatments. $SO_4$ content in leaching water sampled in 1st time was high in T4, but in T3 from 3rd time (p<0.05). Cl, Mg and Na contents were high in leaching water sampled in the 1st time from T4, whereas high in those from T3 in 2nd or 3rd time. Results show that the application of a high DM liquid pig manure is not better for producing Japanese millet and improving the properties of pasture soil than a low DM liquid pig manure. However, the contents of $SO_4$, Cl, Mg and Na in leaching water sampled in 1st time were high in a high DM liquid pig manure.

• Korean Journal of Soil Science and Fertilizer
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• v.38 no.3
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• pp.150-156
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• 2005

The increasing emission of greenhouse gases may change agricultural environment. The agronomic productivity will depend upon change of temperature, precipitation, solar radiation and fertilization. This study was conducted to investigate greenhouse gas emission with irrigation water depth in paddy field. Area of each experiment plot is $70m^2$, Three treatments with three replications were carried out in this experiment, which was laid out as randomized complete block design. The treatments of irrigation water were maximum field water capacity and 4 and 8 cm depth. The application rate of fresh rice straw was $8,000kg\;ha^{-1}$ in combination with chemical fertilizers ($110kg\;N\;ha^{-1}$, $45kg\;P_2O_5\;ha^{-1}$ and $57kg\;K_2O\;ha^{-1}$). The $CH_4$ emission was highest at 32 days after rice transplanting with rice straw treatment. The $CH_4$ emission in the plot of maximum field water capacity was lower compared with 4 and 8 cm of irrigation depth. $CH_4$ and $N_2O$ emission under different water depth in the paddy field were 30 and $1.52kg\;ha^{-1}$ at 8 cm depth, 281 and $1.71kg\;ha^{-1}$ at 4 cm depth, and 219 and $2.01kg\;ha^{-1}$ at water saturated condition. The total emission of greenhouse gases equivalent to $CO_2$ emission with rice straw application were $6,939kg\;CO_2\;ha^{-1}$ at 8 cm depth plot, $6,431kg\;CO_2\;ha^{-1}$ at 4 cm depth plot and $5,222kg\;CO_2\;ha^{-1}$ at water saturated condition. The GWPs without rice straw application were $4,449kg\;CO_2\;ha^{-1}$ at 8 cm depth plot, $3,702kg\;CO_2\;ha^{-1}$ at 4 cm depth plot and $4,579kg\;CO_2\;ha^{-1}$ at water saturated condition.

• Korean Journal of Soil Science and Fertilizer
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• v.28 no.4
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• pp.350-355
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• 1995

This study was conducted to use oyster shells, which have caused environmental problems in the coastal of Korea, as an agricultural material after processing. Physico-chemical components and neutralizing amount on the Ihyun silt loam of crushed oyster shell and slaked lime were examined. In field experiment, the properties of the soil, growth and yield of lettuce, cabbage, spinach, onion, red pepper and soybean were examined by the treatments of the shell(3.68ton/ha) or the lime (2.76ton/ha) with a randomized block design. Particle size of crushed oyster shell consisted of 73.4% of 1~60mesh and 26.6% larger then 61 mesh and contents of CaO, OM, and $P_2O_5$, etc. were 55.5%, 1.3%, and 0.29%, respectively. The requirement of the shell to neutralize the soil was 130~135% of the lime, but after 24months, it was the same. The application of the shell increased the contents of available $P_2O_5$ and $SiO_2$ exchangeable Ca in used soil. The shell tratment increased the leaf height, leaf width, etc. of the examined plants, and the yields 6~154% according to examined plants, as compared with the nonliminged, indicating that the shell possesses a great potential as an agricultural material with the same effectiveness as the lime.

• Journal of Chest Surgery
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• v.30 no.9
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• pp.920-926
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• 1997

Remarkable effect of pain relief and prevention of the postoperative Complications after thoracotomy has been achieved by continuous intravenous analgesia. This study was carried out with thirty patients who underwent posterolateral thoraco tony. The patients were divided into three groups: Group I(n= 10), the patients with intermittent intramuscular analgesia(piroxicam 20 mg), Group II(n=10), the patients with continuous epidural analgesia(0.5% bupivacaine 30m1 + normal saline 30 ml + morphine 10 mg), and Group III(n= 10) the patients with controlled intravenous infusion of analgesics(fentanyl 2500 mfg +normal saline 10 ml). The results w re as follows; 1) There were no significant changes of vital signs, between groups. 2) Tidal volume and FVC were significantly improved in the group II and III compared with the group I during the first postoperative day. 3) A significant reduction of immediate post-thoracotomy pain was achieved in the group II and III compared with the group I. 4) The limitation of motion in the operative side was less in the group II and III compared with the group I. 5) A signi(icant reduction of the postoperative analgegics consumption was noticed in group II and III. 6) Significant complications were not occured during follow-up period in all groups.

• Korean Journal of Poultry Science
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• v.37 no.1
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• pp.1-8
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• 2010

An experiment was conducted to investigate the effect of dietary supplementation of rare earth, individually and in combination with colloidal silver on growth performance, nutrient digestibility, carcass characteristics and immune response in broiler chicks. A total of 3,872 day-old chicks were randomly allotted to four dietary treatments in a randomized complete block design. There were four pens per treatment and 242 chicks per pen. The dietary treatments were T1) CON (control diet), T2) colloidal silver (control diet+20 ppb colloidal silver), T3) rare earth elements (control diet+500 ppm rare earth), T4) colloidal silver +rare earth elements (control diet+20 ppb colloidal silver+500 ppm rare earth). There were no significant differences in feed intake and feed conversion ratio (FCR) among dietary treatments during the whole experimental period (0 to 5 weeks). Body weight gain was greatest in birds fed T3 and T4 diets (p<0.01). Apparent digestibility of dry matter, crude protein, ether extract and calcium were greatest in birds fed T4 diet (p<0.05), while apparent digestibility of phosphorus was lowest in birds fed T3 diet (p<0.05). There was no significant difference in bone strength, carcass characteristics and immune response among dietary treatments. Dry matter content of bone was greatest in birds fed T4 diet (p<0.05), and phosphorus content of bone was greater in birds fed T3 diet than in birds fed T2 and T4 diets (p<0.05). Thus, the results of this study suggest that rare earth elements supplementation, individually and in combination with colloidal silver could improve performance of broilers.

• Korean Journal of Organic Agriculture
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• v.18 no.3
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• pp.401-418
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• 2010

This experiment was selected a $\beta$-glucan producing mushroom strain and developed industrial media, and used to $\beta$-glucan as an alternative for antibiotics in weaned pigs. Yields of mycelial biomass and extracellular $\beta$-glucan from Youngji (Ganoderma lucidum) mushroom was 8.52g/L and 4.49g/L respectively. Also, we prepared optimum formula for mushroom cultivations. A total of 144 pigs ($8.6{\pm}0.9$ kg average body weight, weaned $20{\pm}3$ days of age) were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate in randomized complete block design. Treatments were T1) NC (negative control, basal diet), T2) PC (positive control, basal diet+0.255% antibiotics), T3) NC+0.2% $\beta$-glucan and T4) PC+0.2% $\beta$-glucan. The T2 and T4 treatments were significantly higher in feed efficiency by antibiotics group (p<0.05), however, there was no significant differences in terms of average daily gain (ADG) and average daily feed intake (ADFI) during phase I (0~14 days). In phase II (15~28 days), Pigs fed with antibiotics and $\beta$-glucan (T4) had greater ADG than other treatments (p<0.05), while no differences were observed in ADFI and feed efficiency. During the whole experiment period, the ADG of T4 treatment was higher than other treatments. Pigs fed with $\beta$-glucan (T3 and T4) had greater diarrhea score and moisture content than other treatments (p<0.05). Pigs fed with $\beta$-glucan (T3 and T4) had greater moisture content than other treatments (p<0.05). However, there was no significant differences in diarrhea score and mortality of weaned pigs. There was marginal reductions in feed cost measured feed cost per weight gain used in antibiotics and $\beta$-glucan added diet during phase I. In the second phase, the treatment supplemented with antibiotics had a significantly lower feed cost per weight gain compared to the other treatments. The results from these experiments suggests that $\beta$-glucan is likely able to improve the growth performance, and reduce feed cost although they do not have similar effects like antibiotics in weaning pigs.

• Korean Journal of Soil Science and Fertilizer
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• v.2 no.1
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• pp.31-38
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• 1969

This experiment was carried out to determine the effect of different levels of phosphate and potash firtilizers applied on yields and quality of leaf tobacco. It was done at all varying levels of phosphate and potassium fertilizers $S_0$ (non-fertilizer plot);N:P:K=0:0:0 (Kg/10a) $S_1$ (1/2 amount plot);N:P:K=10:7.5:10 (Kg/10a) $S_2$ (standard plot);N:P:K=10:15:20 (Kg/10a) $S_3$ (1.5 times plot);N:P:K=10:22.5:30(Kg/10a) $S_4$ (2.0times plot);N:P:K==10:30:40 (Kg/10a) Urea was applied as nitrogen, triple superphosphate as phosphate, potassium sulfate as potash source. The total amount of fertilizers was applied as basal dressing per one plant. This experiment was carried out by using randomized block design with 3 replication. The results obtained in terms of yields and quality are summarized as follows. 1. By increasing the amounts of phosphate and potassium fertilizers, growing status of the largest leaf length, the largest leaf width and stem size of tobacco increased in the order of $S_4>S_3>S_2>S_1>S_0$. 2. By increasing the amount of phosphate and potassium fertilizers, yield was increased in the order of $S_4>S_3>S_2>S_1>S_0$, This increase showed generally high significance among various levels of treatments. 3. The regressive equation was derived from the relationship between treatment levels and tobacco leaf yield. The most desirable treatment level for the maximum yield was estimated as the 2.87 times as much as the treatment level of phosphate and potasium fertilizers of standard plot. 4. By increased application of phosphate and potash fertilizers, the contents of $P_2O_5$ and $K_2O$ in the tobacco leaf became greater while the content of nicotine was decreased. Thus, a negative correlation between fertilizer application and nicotine content has been observed.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.569-576
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• 2003

An experiment was conducted to investigate the effect of microbial phytase (Natuphos$^{\circledR}$) supplementation, individually and in combination with carbohydrase enzyme complex (composed of enzymes targeted to SBM dietary components such as $\alpha$-galactosides and galactomannans; ENDO-POWER$^{\circledR}$) to corn-soy basis diet with low nutrient levels on growth performance and nutrient digestibility of growing pigs. A total of 48 crossbred weaned pigs (Landrace${\times}$Yorkshire${\times}$Duroc), 29.1$\pm$0.14 kg of initial body weight, were randomly allotted to four dietary treatments, based on weight and age, according to a Randomized Complete Block Design. There were three pens per treatment and 4 pigs per pen. The dietary treatments were 1) CON (control diet with 3,380 kcal/kg of metabolizable energy, 18.96% of crude protein, 1.10% of lysine, 0.75% of calcium and 0.35% of available phosphorus), 2) LP+NTPS (CON diet with 0.15% unit lower available P levels+0.1% phytase (500 FTU/kg; Natuphos$^{\circledR}$)), 3) LEL+ENP (CON diet with 3.0% unit lower ME and lysine levels + 0.1% carbohydrase enzyme complex (ENDO-POWER$^{\circledR}$), and 4) LPEL+ENZ (CON diet with 0.15% unit lower available P levels and 3.0% unit lower ME and lysine levels+0.1% ENDO-POWER$^{\circledR}$ and 0.1% Natuphos$^{\circledR}$ (500 FTU/kg). There was no significant difference (p〉0.05) in average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) among dietary treatments during the whole experimental period (0 to 4 weeks). Apparent digestibility of gross energy was greater in LP+NTPS and LPEL+ENZ groups than in the LEL+ENP (p<0.05). Apparent digestibility of phosphorus was greater in LP+NTPS than in LEL+ENP (p<0.05). Dry matter excretion was lowest in LPEL+ENZ and phosphorus excretion was lowest in LP+NTPS (p<0.05). Overall, pigs fed on LPEL+ENZ group tended to have better nutrient digestibility (dry matter, gross energy, crude protein and phosphorus) than pigs fed on control group. All dietary enzyme treatment groups showed lower feed cost/body weight gain of pigs than control group. In conclusion, the results from the present study suggest that the simultaneous inclusion of phytase and carbohydrase enzyme complex to diets is advantageous with respect to reducing nutrient excretion of growing pigs and may contribute to increased economic return when added to corn-soy based growing pig diets.

• The Journal of the Korean Society for Microbiology
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• v.21 no.3
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• pp.311-329
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• 1986

The experimental exposure of animals to sources of ultraviolet radiation (UVR) which emit their energy primarily in the UVB region (280-320nm) is known to result in a number of well-described changes in the recipient's immune competence. Two such changes include a depressed capacity to effectively respond immunologically to transplants of syngeneic UVR tumors and a markedly reduced responsiveness to known inducers of delayedtype (DTH) and contact hypersensitivity (CH) reactions. The results of experiments that were designed to elucidate the mechanisms responsible for UVR-induced immunomodulation have implicated: 1) an altered pattern of lymphocyte recirculation, 2) suppressor T cells(Ts), 3) deviations in systemic antigen presenting cell (APC) potential. 4) changes in the production of interleukin-1-like molecules, and 5) the functional inactivation of epidermal Langerhans cells in this process. The exposure of skin to UVR, therefore, causes a number of both local and systemic alterations to the normal host immune system. In spite of this seeming complexity and diversity of responses, our recent studies have established that each of the UVR-mediated changes is probably of equal importance to creating the UVR-induced immunocompromised state. Normal animals were exposed to low dose UVR radiation on their dorsal surfaces under conditions where a $3.0\;cm^2$ area of skin was physically protected from the light energy. Contact sensitization of these animals with DNFB, to either the irradiated or protected back skin, resulted in markedly reduced CH responses. This was observed in spite of a normal responsiveness following the skin sensitization to ventral surfaces of the UVR-exposed animals. Systemic treatment of the low dose UVR recipients with the drug indomethacin (1-3 micrograms/day) during the UVR exposures resulted in a complete reversal of the depressions observed following DNFB sensitization to "protected" dorsal skin while the altered responsiveness found in the group exposed to the skin reactive chemical through directly UVR-exposed sites was maintained. These studies implicate the importance of EC as effective APC in the skin and also suggest that some of the systemic influences caused by UVR exposure involve the production of prostaglandins. This concept was further supported by finding that indomethacin treatment was also capable of totally reversing the systemic depressions in CH responsiveness caused by high dose UVR exposure (30K joules/$m^2$) of mice. Attempts to analyze the cellular mechanisms responsible established that the spleens of all animals which demonstrated altered CH responses, regardless of whether sensitization was through a normal or an irradiated skin site, contained suppressor cells. Interestingly, we also found normal levels of T effector cells in the peripheral lymph nodes of the UVR-exposed mice that were contact sensitized through normal skin. No effector cells were found when skin sensitization took place through irradiated skin sites. In spite of such an apparent paradox, insight into the probable mechanisms responsible for these observations was provided by establishing that UVR exposure of skin results in a striking and dose-dependent blockade of the efferent lymphatic vessels in all peripheral lymph nodes. Therefore, the afferent phases of immune responses can apparently take place normally in UVR exposed animals when antigen is applied to normal skin. The final effector responses, however, appear to be inhibited in the UVR-exposed animals by an apparent block of effector cell mobility. This contrasts with findings in the normal animals. Following contact sensitization, normal animals were also found to simultaneously contain both antigen specific suppressor T cells and lymph node effector cells. However, these normal animals were fully capable of mobilizing their effector cells into the systemic circulation, thereby allowing a localization of these cells to peripheral sites of antigen challenge. Our results suggest that UVR is probably not a significant inducer of suppressor T-cell activity to topically applied antigens. Rather, UVR exposure appears to modify the normal relationship which exists between effector and regulatory immune responses in vivo. It does so by either causing a direct reduction in the skin's APC function, a situation which results in an absence of effector cell generation to antigens applied to UVR-exposed skin sites, inhibiting the capacity of effector cells to gain access to skin sites of antigen challenge or by sequestering the lymphocytes with effector cell potential into the draining peripheral lymph nodes. Each of these situations result in a similar effect on the UVR-exposed host, that being a reduced capacity to elicit a CH response. We hypothesize that altered DTH responses, altered alloresponses, and altered graft-versus-host responses, all of which have been observed in UVR exposed animals, may result from similar mechanisms.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.2
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• pp.92-98
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• 1988

With a purpose of finding out the effects of magnesium and boron enriched organic-compound fertilizer application on the dry matter yield, yield components and changes in the botanical composition on the hilly pasture, a field experiment was arranged with five different treatments as a randomized block desigh and lasted from September, 1984 to the end of growing season in 1986. The results obtained are summarized as follows: 1. As a early plant growth and development, winter hardiness, growth vigour and coverate of grasses at the plots with organic-compound fertilizer application were better comparing to single dressing. 2. Average dry matter yields for two years were shown significantly high due to increasing amount of fertilizer. This trend was same both single and organic-compound fertilizer. And dry matter yields with organic-compound fertilizer application of low (8,693.1 kg/ha) and conventional level (12,758.7 kg/ha) were appeared to increase by 10 and 15% than those of single dressing of Low (7,930.6 kg/ha) and conventional level (11,122.6 kg/ha), respectively. But it was not significant difference. 3. Dry matter yield of grasses was significantly gained by increasing amount of fertilizer. The yield of legumes at the plots with organic-compound fertilizer application the yields of grasses were a litle more increased by 8-14%, and legumes were much more gained by 26-29% than those of the same species groups with single dressing, but it was not significant between the differnet kinds of fertilizer in the same fertilizing level. 4. At the grassland management, the rate of legumes tended to dominate at the plot without fertilization gradually. On the other hand, the botanical compositions and the rates of grasses were much better maintained at the plots with fertilization. But the rates of legumes with organic-compound fertilizer application tended to increase a little more than those of single dressing gradually. 5. All of the soil chemical properties after the experiment were much more improved comparing to before the experiment. But the average contents of soil organic matter and available $P_2O_5$ were slightly higher at the plot with organic-compound fertilization, on the other hand, pH and exchangeable cations tended to a little less than those of single dressing.