• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.76.2-77
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• 2002

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.13-13
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• 2015

Rice blast fungus, Magnaporthe oryzae, is the most destructive pathogen of rice in the world. This fungus has a biotrophic phase early in infection and switches to a necrotrophic lifestyle after host cell death. During the biotrophic phase, the fungus competes with host for nutrients and oxygen. Continuous uptake of oxygen is essential for successful establishment of blast disease of this pathogen. Here, we report transcriptional responses of the fungus to oxygen limitation. Transcriptome analysis using RNA-Seq identified 1,047 up-regulated genes in response to hypoxia. Those genes were involved in mycelial development, sterol biosynthesis, and metal ion transport based on hierarchical GO terms and well-conserved among three different fungal species. In addition, null mutants of three hypoxia-responsive genes were generated and tested for their roles on fungal development and pathogenicity. The mutants for a sterol regulatory element-binding protein gene, MoSRE1, and C4 methyl sterol oxidase gene, ERG25, exhibited increased sensitivity to hypoxia-mimetic agent, increased conidiation, and delayed invasive growth within host cells, suggesting important roles in fungal development. However, such defects did not cause any significant decrease in disease severity. The other null mutant for alcohol dehydrogenase gene, MoADH1, showed no defect in the hypoxia-mimic condition and fungal development. Taken together, this comprehensive transcriptional profiling in response to a hypoxia condition with experimental validations would provide new insights on fungal development and pathogenicity in plant pathogenic fungi.

• Journal of the Korean Institute of Gas
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• v.6 no.1 s.17
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• pp.46-51
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• 2002

In subway or road construction, the vibration caused by various construction equipments influences gas pipelines directly or indirectly Especially buried gas pipelines are influenced by the blast occurred near the pipeline buried Place. To analyze vibration response of buried gas pipeline caused by blasting works, the nonlinear behavior of ground is realized by applying equivalent linear analysis. According to the results of this analysis, the acceleration response values of gas pipeline are close to the measured values and the occurring time of peak values are agreed to the measured values. Thus, It is concluded that conventional seismic analysis mechanism can be applied to the dynamic analysis of buried gas pipeline.

• Explosives and Blasting
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• v.29 no.1
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• pp.41-47
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• 2011

In order to estimate blast damage zone and control rock fragmentation in blasting, it is important to obtain information regarding blast hole pressure. In this study, drop impact tests of acrylic, aluminium, steel sensors were performed to investigate the dynamic response characterizations of the sensors through the strain signals. As a result, the strain signals obtained from the steel sensors showed less sensitivity to impact force level and experienced small changes with various length of the sensors. The steel sensors were applied to measure the impact force of an electric detonator.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2004.10a
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• pp.48-52
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• 2004

We have found the role of rice mitogen-activated protein kinase kinase kinase (MAPKKK), OsEDR1, as controling hypersensitive response (HR) and increased disease resistance to rice blast fungus Magnaporthe grisea. Generation of transgenic rice plants through introduction of the over-expression construct of OsEDR1 using Agrobacterium-mediated transformation results in lesion mimic phenotype. Up-regulation of defense mechanism was detected through detection of increased transcription level of rice PBZ1 and PR1a. Inoculation of rice blast fungus on the lesion mimic transgenic lines displayed significantly increased resistance. The disease symptoms were arrested like HR responses which are commonly detected in the incompatible interactions. High accumulation of phenolic compounds around developing lesions was detected under UV light. There was variation among transgenic lines on the timing of lesion progression as well as the lesion numbers on the rice leaves. Transgenic lines with few lesions also show increased resistance as well as equal amount of grain yields compared to that of wild type rice cultivar Nipponbare. This is the first report of the MAPKKK as a positive regulator molecule on defense mechanism through inducing HR-like cell death lesion mimic phenotype. The application of OsEDR1 is highly expected for the development of resistant cultivars against rice pathogens.

• International Journal of Concrete Structures and Materials
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• v.1 no.1
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• pp.29-36
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• 2007

The purpose of this paper is the assessment of the capacity of the reinforced concrete (RC) elements of an arch bridge when they are subjected to contact and near-contact explosive charges of various amounts, and the estimation of the critical charges for these components. The bridge considered is the Tenza Viaduct, a decommissioned structure south of Naples, Italy. Its primary elements, deck, piers and arches were analyzed. The evaluation was accomplished via numerical analyses that made possible to obtain the elements dynamic response when they are exposed to blast loading conditions. To evaluate the member's capacities, failure criteria for deck, piers and arches were proposed based on concrete damage parameters. Additionally, curves relating the explosive charge to the residual capacity and to damage level of the elements were also developed. The results of this work were taken into account to investigate the progressive collapse of the global structure.

• Advances in materials Research
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• v.6 no.2
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• pp.155-168
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• 2017

The terrorist attacks and dangers by bomb blast have turned into an emerging issue throughout the world and the protection of the people and structures against terrorist acts depends on the prediction of the response of structures under blast and shock load. In this paper, behavior of aluminum and unidirectionally reinforced E-Glass Epoxy composite plates with and without focal circular holes subjected to shock loading has been identified. For isotropic and orthotropic plates (with and without holes) the classical normal mode approach has been utilized as a part of the processing of theoretical results. To obtain the accurate results, convergence of the results was considered and a number of modes were selected for plate with and without hole individually. Using a shock tube as a loading device, tests have been conducted to composite plates to verify the theoretical results. Moreover, peak dynamic strains, investigated by experiments are also compared with the theoretical values and deviation of the results are discussed accordingly. The strain-time histories are likewise indicated for a specific gauge area for aluminum and composite plates. Comparison of dynamic-amplification factors between the isotropic and the orthotropic plates with and without hole has been discussed.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2019.05a
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• pp.182-183
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• 2019

In this study, the optimization of mixing proportion of press-forming board with blast furnace slag, pearlite and bottom ash was investigated using the response surface methodology. Ten Mixing proportions of specimens were designed by the response surface design, and then flexural failure load, moisture content and water absorption of specimens were measured. As a result of the reaction surface analysis based on the experimental results, it was possible to derive the optimal mixing proportion with the satisfaction of 93%.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.2
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• pp.197-205
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• 2015

Offshore structures for the gas production are exposed to the risk of gas leaks, and gas explosions can result in fatal damages to the primary structures as well as secondary structures. To minimize the damage from the critical accidents, the study of the dynamic response of structural members subjected to blast loads must be conducted. Furthermore, structural dynamic analysis has to be performed considering relationships between the natural frequency of structural members and time duration of the explosion loading because the explosion pressure tends to increase and dissipate within an extremely short time. In this paper, the numerical model based on time history data were proposed considering the negative phase pressure in which considerable negative phase pressures were observed in CFD analyses of gas explosions. The undamped single degree of freedom(SDOF) model was used to characterize the dynamic response under the blast loading. A blast wall of FPSO topside was considered as an essential structure in which the wall prevents explosion pressures from the process area to utility and working areas. From linear/nonlinear transient analyses using LS-DYNA, it was observed that dynamic responses of structures were influenced by significantly the negative time duration.

• Resources Recycling
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• v.21 no.3
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• pp.39-47
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• 2012

This study was conducted to optimize a mixing design of lightweight aerated concrete with the blast furnace slag(BFS) using Box-Behnken method, one of response surface designs. The lightweight aerated concrete with the BFS was made on the conditions of steam curing method at atmospheric pressure. The experimental factors were unit Water(W)/total powder($P_d$) ratio, BFS replacement percentage and Al powder addition based on the total powder (${P_d}^*$%). From the results of the response surface analysis, regression models for dried specific gravity and compressive strength of the lightweight aerated concrete were derived. When the target values for dried specific gravity and compressive strength of the lightweight aerated concrete were set at 0.72 and 4.42 MPa respectively, its optimized mixing conditions driven from the regression models were 0.62 of $W/P_d$ ratio, 35.5% of BFS replacement and 0.05% of Al powder addition. This experimental design model was found to be credible by measuring the dried specific gravity and compressive strength of the sample made from the above mixing conditions.