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Variation of Grain Quality and Grain Filling Rapidity Milyang 23 / Gihobyeo Recombinant Inbred Lines (벼 밀양 23호$\times$기호벼의 재조합 자식계통에서 초기급속등숙과 미질 특성)

  • 곽태순;여준환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.3
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    • pp.160-166
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    • 2004
  • This study was carried out to get the basic informations regarding the varietal variations for the physicochemical properties such as protein content, amylose content, fatty acid content, grain quality values and color properties such as lightness value, chroma and hue for the 164 recombinant inbred lines(RILs) of Milyang 23 and Gihobyeo(M/G) at the experimental farm in the Sangji University. The principal component analysis and heritability study were conducted for this experiments. The rapidity of grain filling(RCF) for the 164 M/G RILs could be classified into four groups such as slow maturing group less than 41%, mid-slow maturing group 41∼60%, fast maturing group 61∼80% and very fast maturing group more than 81% based on the rapidity of grain filling rate. The slow maturing group of RGF showed a little bit higher protein content 9.1%, compared to the other RGF groups. However, the amylose content of all the RGF groups revealed the same content by the groups. The very fast maturing group of RGF showed longer grain length in brown rice compared to other RGF varietal groups, in case of grain width in brown rice showed shorter than any other groups. The alkali digestive value which was so much related to gelatinization temperature showed 3.40 degree at fast maturing group of RGF in M/G RILs. However, the very fast maturing group of RGF revealed 4.31 degree of alkali digestive value. The principal component analysis was performed by the chemical and color properties such as quality value, protein content, amylose content, alkali digestive value, fatty acid content, lightness value, chroma and hue for M/G RILs. The first principal component was able to explained upto 36% to total informations. It was corresponded to quality value, protein content, amylose content, fatty acid content, lightness value and a-value(green -1 red). The characters regarding grain quality showed high heritable properties more than 75% of heritability, but color characters appeared relatively lower heritability compared to grain quality.

Biogeochemical Remediation of Cr(VI)-Contaminated Groundwater using MMPH-0 (Enterobacter aerogenes) (MMPH-0 (Enterobacter aerogenes)에 의한 6가 크롬 오염 지하수의 생지화학적 정화)

  • Seo, Hyun-Hee;Rhee, Sung-Keun;Kim, Kang-Joo;Park, Eun-Gyu;Kim, Yeong-Kyoo;Chon, Chul-Min;Moon, Ji-Won;Roh, Yul
    • Economic and Environmental Geology
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    • v.45 no.2
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    • pp.105-119
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    • 2012
  • Indigenous bacteria isolated from contaminated sites play important roles to remediate contaminated groundwater. Chromium has the most stable oxidation states. Cr(VI) is toxic, carcinogenic, and mobile, but Cr(III) is less toxic and immobile. In this study, indigenous microorganism (MMPH-0) was enriched from Cr(VI) contaminated groundwater, and identified by 16S rRNA gene analysis. Using MMPH-0, the effect of stimulating with e-donors (glucose, lactate, acetate, and no e-donor control), respiration conditions, biomass, tolerance, and geochemical changes on Cr(VI) reduction were investigated in batch experiments for 4 weeks. The changes of Cr(VI) concentration and geochemical conditions were monitored using UV-vis-spectrophotometer and Eh-pH meter. And the morphological and chemical characteristics of MMPH-0 and precipitates in the effluents were characterized by TEM-EDS and SEM-EDS analyses. MMPH-0 (Enterobacter aerogenes) was able to tolerate up to 2000 mg/L Cr(VI) and reduce Cr(VI) under aerobic and anaerobic conditions. MMPH-0 performed faster and higher efficiency of Cr(VI) reduction with electron donors (over 70% after 1 week with e-donor, 10-20% after 4 weeks without e-donor). The changes of Eh-pH in effluents showing the tendency from oxidizing to reducing condition and a bit of acidic change in pH due to microbial oxidation of organic matters donating electrons and protons suggested the roles of MMPH-0 on Cr(VI) in the contaminated water catalyzing to transit geochemical stable zone for more stable $Cr(OH)_3$ or Cr(III) precipitates. TEM/SEM-EDS analyses of MMPH-0 and precipitates indicate direct and indirect Cr(VI) reduction: extracellular polymers capturing Cr component outside cells. These results suggested diverse indigenous bacteria and their biogeochemical reactions might enhance more effective and feasible remediation technology of redox sensitive heavy metals in metal-contaminated in groundwater.

Processing and Property of Olive Flounder Paralichthys olivaceus Steak (넙치(Paralichthys olivaceus)스테이크제품의 제조 및 품질특성)

  • Yoon, Moon-Joo;Kwon, Soon-Jae;Lee, Jae-Dong;Park, Si-Young;Kong, Cheong-Sik;Joo, Jong-Chan;Kim, Jeong-Gyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.1
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    • pp.98-107
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    • 2015
  • Olive flounder contains rich amount of lysine which is required for children's growth. Moreover, it is good foodstuffs for elderly, convalescent and diabetics because of low lipid content and high digestibility. This study was investigated for the purpose of obtaining basic data which can be applied to the processing of olive flounder steak. Olive flounder 100 g were chopped, mixed with vegetable (onion 20%, celery 10%, carrot 15%, garlic 1% of chopped olive flounder meat) and ingredient (bread crumbs 20 g, onion 15 g, celery 10 g, egg 1 ea, tarragon 1/2 t, blanc sauce 20 g, fresh cream 20 mL, salt and pepper pinch). Mixed dough was molded into steak shape ($12{\times}7cm$) and was processed by two types of products, Steak-1 {Roasting for 2 minutes in a frying pan wrapped with olive oil and then vacuum packaging in polyethylene film ($20{\times}30{\times}0.05mm$), and then storage at $-20^{\circ}C$ for 7 days, next thawed and warmed by microwave for 2 minutes} and Steak-2 {vacuum-packaging in polyethylene film ($20{\times}30{\times}0.05mm$), and then storage at $-20^{\circ}C$ for 7 days, after thawed, roasted during 2 minutes in a frying pan wrapped with olive oil}. The factors such as pH, TBA value, amino-N, free amino acid, chemical composition, color value (L, a, b), texture profile, sensory evaluation and viable bacterial count of the olive flounder steak (Steak-1, Steak-2) were measured. From the result of sensory evaluation, Steak-2 showed a bit more high scores than Steak-1 but it was difficult to distinguish significant difference (color, odor, taste, texture and acceptance) between Steak-1 and Steak-2 products.

Bulb Propagation on Nerine by Tissue Culture (조직배양에 의한 네리네 대량증식)

  • Han, Soo-Gon;Kang, Chan-Ho;Choi, In-Young;Choi, So-Ra;Lim, Hoi-Chun;Lee, Jin-Je;Oh, Nam-Ki;Choi, Jeong-Sik
    • Korean Journal of Plant Resources
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    • v.21 no.2
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    • pp.134-138
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    • 2008
  • Nerine from south africa and its sparkling flower shape make us estimate it as a hopeful kind of cut follow. There was a few studies on Nerine in korea. We started this study to set bulb propagation methods. The propagation by tissue culture was changeable according to the growth regulators The best growth regulator combination which makes a lot of Bulblet was NAA $0{\sim}0.5$ + BA $0.5{\sim}2.0mg\;{\cdot}\;L^{-1}$ in Nerine bowdenii ‘Favourite’ and Nerine sarniens ‘Red’ respectively. The adjust culture media source for tissue culture were glucose 9% as a carbon source and ($NH_4+NO_3$) 40mM as a nitrogen source. When glucose was used as a carbon source, Bulblet were harvested a little bit low then sucrose but comparative emergence rate was so high that it is good for carbon source in nerine tissue culture. When we consist culture media as MS+BA $1.0mg\;{\cdot}\;L^{-1}$+sucose 7% + ($NH_4+NO_3$) 40mM, the produced Bulblet were reached up to 1.7 each per bulb and emergence rate was up to 100% irrespective of acclimatization period. The suitable culture explant for nerine tissue culture was scale. When scale was cultured with MS+BA $1.0mg\;{\cdot}\;L^{-1}$+sucose 7%, its propagation efficiency was 54 times greater than using growing point. A proper culture part of the scaly leaf was middle part (8 scaly leaf from outer 8th scaly leaf) when middle part was cultured the number of Bulblet were up to 1.8 each per explant.

Effects of Chilling and Overwintering Temperature Conditions on the Termination of Egg Diapause of the Ussur Brown Katydid Paratlanticus ussuriensis (갈색여치 알의 휴면타파를 위한 저온처리 및 월동 온도조건의 영향)

  • Bang, Hea-Son;Kim, Myung-Hyun;Jung, Myung-Pyo;Han, Min-Su;Na, Young-Eun;Kang, Kee-Kyung;Lee, Deog-Bae;Lee, Kyeong-Yeoll
    • Korean journal of applied entomology
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    • v.48 no.2
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    • pp.221-227
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    • 2009
  • Temperature effects on diapause termination of Paratlanticus ussuriensis eggs were studied by measuring embryonic development and hatching rates at various conditions of indoor chilling and overwintering temperatures. Diapausing eggs of P. ussuriensis did not hatch at continued incubation at $25^{\circ}C$ and even after chilling for once at either $5^{\circ}C$ or $10^{\circ}C$ for 30, 45 and 60 days. In addition, double chillings at $5^{\circ}C$ with a 90 days interval at $25^{\circ}C$ did not induce hatching of diapausing eggs. However, double chillings at $10^{\circ}C$ induced hatching at 3.6${\sim}$26.7%. When eggs were incubated at $25^{\circ}C$ after chilling for once at $5^{\circ}C$ for various periods, those weights were not changed but those chilled at $10^{\circ}C$ gradually increased to approximately 1.5 times. When 60-days-old eggs were artificially deposited under the soil at three different mountain sites in September 2007, the hatching rates of the first-overwintered eggs were 11.3, 3.5 and 4.1% and those of the second-overwintered eggs were 25.1, 21.6 and 0.4% at Hoepori, Bitanri and Hwasanri, respectively. Most eggs were hatched from mid-March to mid-April but little bit earlier in southern regions. During the hatching period soil temperatures in three tested locations were around 8 to $12^{\circ}C$. In overall, diapausing eggs of P. ussuriensis were greatly influenced by chilling temperature conditions and those repeated cycles, and may required overwintering for one or two times to hatch for the post-embryonic development.

Barium Compounds through Monte Carlo Simulations Compare the Performance of Medical Radiation Shielding Analysis (몬테카를로 시뮬레이션을 통한 바륨화합물의 의료방사선 차폐능 비교 분석)

  • Kim, Seonchil;Kim, Kyotae;Park, Jikoon
    • Journal of the Korean Society of Radiology
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    • v.7 no.6
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    • pp.403-408
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    • 2013
  • This study made a tentative estimation of the shielding rate of barium compound by thickness through monte carlo simulation to apply medical radiation shielding products that can replace existing lead. Barium sulfate($BaSO_4$) was used for the shielding material, and thickness of the shielding material specimen was simulated from 0.1 mm to 5 mm by applying $15{\times}15cm^2$ of specimen area, $4.5g/cm^3$ of density of barium sulfate, and $11.34g/cm^3$ density of lead. Entered source was simulated with 10kVp Step in consecutive X-ray energy spectrum(40 kVp ~ 120 kVp). Absorption probability in 40 kVp ~ 60 kVp showed same shielding rate with lead in 3 mm ~ 5 mm of thickness, but it was identified that under 2 mm, the shielding rate was a bit lower than the existing lead shielding material. Also, the shielding rate in 70 kVp ~ 120 kVp energy band showed similar performance as the existing lead shielding material, but it was tentatively estimated as fairly low shielding rate below 0.5 mm. This study estimated the shielding rate of barium compound as the thickness function of x-ray energy band for medical radiation through monte carlo simulation, and made comparative analysis with existing lead. Also, this study intended to verify application validity of the x-ray shielding material for medical radiation of pure barium sulfate. As a result, it was estimated that the shielding effect was 95% higher than the existing lead 1.5 mm in at least 2 mm thickness of barium compound in medical radiation energy band 70 kVp ~ 120 kVp, and this result is considered valid to be provided as a base data in weight lightening production of radiation shielding product for medical radiation.

Analytical studies of bovine mastitis management by standard plate counts(SPC) and somatic cell counts(SCC) (젖소 유방염 관리에 따른 세균 및 체세포수 등급 실태 조사 분석)

  • 허정호;정명호;박영호;조명희;이주홍
    • Korean Journal of Veterinary Service
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    • v.21 no.3
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    • pp.285-300
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    • 1998
  • 1. The number of average milking cows, clinical forms of mastitis, mastitis-developing cows, and cows killed by mastitis a year were 25.7, 1.8(7%), 6.3(26%), and 2.7(10.1%)heads, respectively. The annual grade changes of standard plate counts(SPC) and somatic cell counts(SCC) showed the grade 1A of SPC diminished sharply from April to August, we think it was due to the lack of proper management in farming season and the grade 3 of SCC indirectly influenced increased in huge during August. 2. The average number of parturitions of farms was 2.3, but 50% of below 1 parturition were 22 farms(31%), 50% of above 3 parturitions were 16(23%) out of 71 farms. According to grades of the number of parturitions of milking cows per each farm, the farms' grades recording 3 parturitions and 50% were little bit excellent. 3. The actual situation research of foremilking CMT revealed 35 out of 74 farmer didn't do CMT Among them(35 out of 74 farmers), 80% did not test thanks to the troublesome process of the CMT. SCC grade 3, among farms who did foremilking CMT once or twice a month and who did not were 29% and 40% respectively and SPC grade 1A were 55% and 9%, respectively. 4. The research of actual situation on milking management let us know 29 farms(39%) did not do lastmilking, 37 farms(49%) usually did overmilking, and 34 farms(46%) did milking for 4 or 5 minutes. Grades according to average requiring times of milking showed SCC grade 1 of farms milking within 7 minutes was 11% and SPC grade 1A was 34%, on the other side, farms milking more than 7 minutes were 0% in SCC grade 1 and 13% in SPC grade 1A. Grades according to the starting time of milking after rubbing teats showed SPC grade 1A of farms starting milking at about 1 minute and over 2 minutes were 50% and 20%, respectively. 5. The research of actual situation on hygienic milking management uncovered 65 farms(88%) were using one towel which was used in washing teats and udders to wash more than 3 to 4 cows, and 53 farms(72%) were using one dried towel to dry udders not for each cow but for more than 3 to 4 cows after washing. Also, on milking turns disclosed 30 farms(40%) were milking cows in the order of incoming without isolation of a dominant group. According to grades of towels used in washing teats and udders, farms using a towel for each cow were 56% and a towel for over 3 cows were 31% in SPC grade 1A. According to using-or-not grades of dried towels after washing udders, farms using a towel for each cow were 79% and a towel for over 3 cows were 21% in SPC grade 1A. 6. Farms doing teat-dipping before milking were 7(10%), not doing teat-dipping after milking, or doing sometimes were 9(12%), and doing right after milking were 57(77%). And farms doing teat-dipping after dry cows and before delivery were 21(28a ). Farms using bethadine as an antiseptic solution were 70(95%), 40 farms(59%) diluted it with water as weak as 5 to 10 times, and on drying cows 64 farms(87%) slowly did it more than 2 days. Grade 1A of SPC of farms doing teat-dipping at every milking was 38%, farms doing occasionally or not was 33%, and farms doing it right after milking was 37% and doing after milking more than 5 cows was 20%. Grade 1A of SPC among farms diluting bethadine 5 times and diluting 5 to 10 times with water were 36% and 33%, respectively, and Grade 3 of SCC were 35% and 32%, respectively. 7. Studies on nonlactating period medical treatment, as the cows were on dry, 54 farms treated with their own hands.73 farms(98%) had bovine mastitis treated for themselves. And on applying medicines against mastitis, 55 farmers chose them on the basis of their own experience, 42 farms(57%) were treated more than 3 days. 41 farms(55%) dumped away the mastitis infected milk separately, 24 farms(32%) were feeding and milking at the same time. 8. Fifty-six farms(76%) always washed and disinfected milking machines after milking. Farms using the milking machines at low, or variable vacuum pressures, or at the vacuum pressure, set at the moment of its installation were 31(42%), and farms that did not know pulsation ratio were 27(37%). Farms changing liners when they were torn 8(11%), 58 farms(78%) said they checked milking system when there were wrong with them, 31 farms(42%) changed milking hoses when they found out problems, and 42 farms(57%) cleaned vacuum and milking systems when they felt dirty. The SPC grade 1A of farms washing and sterilizing milking machines was 38% and farms only washing was 28%.

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A study on correlation of teaching efficiency and satisfaction of clinical training in Daegu (임상실습교육의 교수효율성과 임상실습만족도에 관한 상관성 연구 (대구지역을 중심으로))

  • Kim, Jeong-Sook;Jung, Young-Hae
    • Journal of Technologic Dentistry
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    • v.28 no.1
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    • pp.121-142
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    • 2006
  • Collecting materials for study on teaching efficiency and satisfaction of clinical training, it changes. Dental technology's educational procedure to many ways of a prospect. In a circumstance that needed higher level of education, this study is aimed on realizing an importance of clinical training through the various materials that previously carried out and offering basic knowledge to take better clinical training for the students. Study results below 1. This Investigation conducted on 123 of sophomores(70.3%) and 52 of juniors(29.7%) who have been taken clinical training, and men's proportion(51.45%)is a bit higher than girls(48.6%). The 64% of respondents taken largest proportion were 20 to 24 years old. As 67.9% of respondents attended daytime school and 30.3% of them attended nighttime one, their school time shows a little difference. In a question about relation ship, one answered "Harmonious" took largest proportion by 72.6% during training, and about the degree of satisfaction of campus life who answered "normal" were the most with 59.4%. 2. About the reason choosing dental technology as a major, 41.1% taken the most answered "due to the specialized job", "Getting job easily" was second with 26.9%, and third was "recommended from around" with 18.3%. 50.3% of the respondents answered "normal" about the Satisfaction of their major, student marked in grade "B" most with 51.4% 3. In a investigation result about clinical training statues and preference, most(72.6%) choose place less than 10 for clinical training, and 60.6% of them resided own home. About their commuting time from home to training place, 44% was under 30min, 40% took time 30-60min. It shows students prefer shotter distance in terms of choosing training place. 4. Each part manager took large proportion as a clinical trainer with 33.7%, Training curriculum reform and developing method were most answer as a improvement measure after completing training with 30%. 5. The average of total score about clinical training was 3.15 of 5. In the detailed question, 'satisfaction of clinical training' got 3.38 as a highest score, the lowest score was 2.86 that is about satisfaction of clinical training period. The average score about efficiency of study was 2.86 and in detailed question, 'a Role model' got 3.26 as a highest score and participation of student got 3.05 as a lowest score. 6. The result of T-test to see the difference of the satisfaction according to the general character and clinic training condition between teaching efficiency is that the degree of satisfaction of clinical training showed statistical significance only in the degree of satisfaction of campus life(p<0.05), and teaching efficiency has a statistical significance with their age, grade, and satisfaction of campus life (p<0.05). 7. The relation between of teaching efficiency of clinical training and satisfaction of clinical training of dental technologic student has a statistical meaning in significance leveler 0.01. Now, therefore we suggest following based on these result. 1. To elevate satisfaction of clinical training, it agentry needs development of consistent clinical training curriculum. 2. To grasp the satisfaction and requirement, in needs to measure anxiousness and satisfactory degree after completing training 3. To train efficiently and evaluate efficiency over the teaching activities, it needs to develop measuring tools for teaching efficiency in terms of teacher's important rules in a clinical training. 4. Strengthen the relations with the study developing and managing curriculum gathering theoretical knowledge and practice. And make an effort to apply to their students. 5. Let the trainee take a class setting a belief, sense of value, function and obtain behavior by making the students comfort over clinical training as increasing teaching efficiency.

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Characteristics and Screening of Antioxidative Activity for the Fruit by Rubus coreanus Miq. Clones (복분자딸기 클론별 과실특성과 항산화 활성 탐색)

  • Kim, Sea Hyun;Chung, Hun Gwan;Jang, Yong Seok;Park, Young Ki;Park, Hyung Soon;Kim, Sun Chang
    • Journal of Korean Society of Forest Science
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    • v.94 no.1 s.158
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    • pp.11-15
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    • 2005
  • Fruit characteristics, saccharinity, and antioxidative activity of selected clones of Rubus coreanus, grown in Korea, well-known as edible and medical resources, frequently used and which has an excellent pharmacological activity are examined and analyzed. The results obtained from this study can be summarized as follows; Result of surveying characteristics for the fruit and saccharinity variation by collection time, there are some meaningful differences between examined clones and the collection time, exception of the fruit width. From the clones, SG 3 is the biggest in size, the highest in the saccharinity out of five clones, on the other hand, CJ 18 and SH 3 are the opposite. Meanwhile, comparing examined saccharinity factor with maximum temperature and precipitation out of daily climates at Suwon Meteorological Observatory at the same period, on the day the highest temperature is $29.5^{\circ}C$, saccharinity distinctively ascends, but on the day the precipitation is recorded 121.5 mm and next day, the saccharinity descends. Also, five-day saccharinity until the precipitation lower than 0.1 mm per day shows from 10.4 to 11.5 Brix higher than the average saccharinity of 9.7 Brix. Fifteen clones including CA 6 about fruit characteristics in terms of collection layer and saccharinity variation were examined, there is significantly difference among examined clones, but about the collection layer, only saccharinity factor has meaningful significance. For clones, MJ 11 is the biggest and excellent character of 15 clones but the saccharinity, 10.3 Brix lows a bit, MC 9 and HAE 5 are the worst fruit in character but saccharinity is 13.4 Brix and 10.6 Brix which is higher than the average saccharinity of 9.8 Brix. About saccharinity for the collection layer, the fruit average saccharinity, collected from the upper layer which is good at lightness and temperature condition, is 10.2 Brix which shows higher than the one of the fruit, 9.7 Bix and 9.5 Brix collected from the middle or the lower layer. The saccharinity of fruit collected from the middle or the lower layer is lower than the average saccharinity of 9.8 Brix out of 15 surveyed clones. Surveying antioxidative activity resulted from free radical scavenging activity about 30 clones of fruit in total, there is difference between surveyed clones. Especially, Immatured fruit extracts exhibited higher free radical scavenging activity of 94.5 to 97.5% which indicates the similarity to Ascorbic acid, unlike other clones, on the concentration of 250 to 1,000 ppm.

A New Bias Scheduling Method for Improving Both Classification Performance and Precision on the Classification and Regression Problems (분류 및 회귀문제에서의 분류 성능과 정확도를 동시에 향상시키기 위한 새로운 바이어스 스케줄링 방법)

  • Kim Eun-Mi;Park Seong-Mi;Kim Kwang-Hee;Lee Bae-Ho
    • Journal of KIISE:Software and Applications
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    • v.32 no.11
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    • pp.1021-1028
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    • 2005
  • The general solution for classification and regression problems can be found by matching and modifying matrices with the information in real world and then these matrices are teaming in neural networks. This paper treats primary space as a real world, and dual space that Primary space matches matrices using kernel. In practical study, there are two kinds of problems, complete system which can get an answer using inverse matrix and ill-posed system or singular system which cannot get an answer directly from inverse of the given matrix. Further more the problems are often given by the latter condition; therefore, it is necessary to find regularization parameter to change ill-posed or singular problems into complete system. This paper compares each performance under both classification and regression problems among GCV, L-Curve, which are well known for getting regularization parameter, and kernel methods. Both GCV and L-Curve have excellent performance to get regularization parameters, and the performances are similar although they show little bit different results from the different condition of problems. However, these methods are two-step solution because both have to calculate the regularization parameters to solve given problems, and then those problems can be applied to other solving methods. Compared with UV and L-Curve, kernel methods are one-step solution which is simultaneously teaming a regularization parameter within the teaming process of pattern weights. This paper also suggests dynamic momentum which is leaning under the limited proportional condition between learning epoch and the performance of given problems to increase performance and precision for regularization. Finally, this paper shows the results that suggested solution can get better or equivalent results compared with GCV and L-Curve through the experiments using Iris data which are used to consider standard data in classification, Gaussian data which are typical data for singular system, and Shaw data which is an one-dimension image restoration problems.