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http://dx.doi.org/10.9719/EEG.2012.45.2.105

Biogeochemical Remediation of Cr(VI)-Contaminated Groundwater using MMPH-0 (Enterobacter aerogenes)  

Seo, Hyun-Hee (Department of Earth and Environmental Sciences, Chonnam National University)
Rhee, Sung-Keun (Department of Microbiology, Chungbuk National University)
Kim, Kang-Joo (Department of Environmental Engineering, Kunsan National University)
Park, Eun-Gyu (Department of Geology, Kyungpook National University)
Kim, Yeong-Kyoo (Department of Geology, Kyungpook National University)
Chon, Chul-Min (Geologic Environment Division, Korea Institute of Geosciences and Mineral Resources)
Moon, Ji-Won (Biosciences Division, Oak Ridge National Laboratory)
Roh, Yul (Department of Earth and Environmental Sciences, Chonnam National University)
Publication Information
Economic and Environmental Geology / v.45, no.2, 2012 , pp. 105-119 More about this Journal
Abstract
Indigenous bacteria isolated from contaminated sites play important roles to remediate contaminated groundwater. Chromium has the most stable oxidation states. Cr(VI) is toxic, carcinogenic, and mobile, but Cr(III) is less toxic and immobile. In this study, indigenous microorganism (MMPH-0) was enriched from Cr(VI) contaminated groundwater, and identified by 16S rRNA gene analysis. Using MMPH-0, the effect of stimulating with e-donors (glucose, lactate, acetate, and no e-donor control), respiration conditions, biomass, tolerance, and geochemical changes on Cr(VI) reduction were investigated in batch experiments for 4 weeks. The changes of Cr(VI) concentration and geochemical conditions were monitored using UV-vis-spectrophotometer and Eh-pH meter. And the morphological and chemical characteristics of MMPH-0 and precipitates in the effluents were characterized by TEM-EDS and SEM-EDS analyses. MMPH-0 (Enterobacter aerogenes) was able to tolerate up to 2000 mg/L Cr(VI) and reduce Cr(VI) under aerobic and anaerobic conditions. MMPH-0 performed faster and higher efficiency of Cr(VI) reduction with electron donors (over 70% after 1 week with e-donor, 10-20% after 4 weeks without e-donor). The changes of Eh-pH in effluents showing the tendency from oxidizing to reducing condition and a bit of acidic change in pH due to microbial oxidation of organic matters donating electrons and protons suggested the roles of MMPH-0 on Cr(VI) in the contaminated water catalyzing to transit geochemical stable zone for more stable $Cr(OH)_3$ or Cr(III) precipitates. TEM/SEM-EDS analyses of MMPH-0 and precipitates indicate direct and indirect Cr(VI) reduction: extracellular polymers capturing Cr component outside cells. These results suggested diverse indigenous bacteria and their biogeochemical reactions might enhance more effective and feasible remediation technology of redox sensitive heavy metals in metal-contaminated in groundwater.
Keywords
indigenous bacteria; Enterobacter aerogenes; Cr(VI); biogeochemistry; reduction-oxidation reaction;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Cheung, K.H. and Gu, J.D. (2007) Mechanism of hexavalent chromium detoxification by microorganisms and bioremediation application potential: A review, International Biodeterioration & Biodegradation, v.59, p.8-15.   DOI
2 Chon, C.-M., Ahn, J.S., Roh, Y., Rhee, S.-K., Seo, H., Kim, G.-Y., Koh, D.-C., Son, Y. -C. and Kim, J.-W. (2006) Seasonal variation of Cr(VI)-contaminated groundwater quality and the potential for natural attenuation. Econ. Environ. Geol., v.41(6), p.645-655.   과학기술학회마을
3 Chon, C.-M., Moon, S.H., Ahn, J.S., Kim, Y., Won, J.-H. and Ahn, K.-H. (2007) Fate and transport of Cr(VI) contaminated groundwater from the industrial area in Daejeon. Econ. Environ. Geol., v.40(4), p.403-417.   과학기술학회마을
4 Cummings, D.E., Fendorf, S., Singh, N., Sani, R.K., Peyton, B.M. and Magnuson, T.S. (2007) Reduction of Cr(VI) under acidic conditions by the facultative Fe(III)-reducing bacterium Acidiphilium cryptum, Environmental Science Technology, v.41, p.146-152.   DOI
5 Daulton, T.L., Little, B.J., Jones-Meehan, J., Blom, D.A. and Allard, L.F. (2007) Microbial reduction of chromium from the hexavalent to divalent state, Geochimica et Cosmochimica Acta, v.71, p.556-565.   DOI
6 Ellis, A.S., Johnson, T.M. and Bullen, T.D. (2002) Chromium isotopes and the fate of hexavalent chromium, Science v.295(5562), p.2060-2062.   DOI
7 Francis, C.A., Obraztsova, A.Y. and Tebo, B.M. (2000) Dissimilatory metal reduction by the facultative anaerobe Pantoea agglomerans SP1, Applied and Environmental Microbiology, v.66(2), p.543-548.   DOI   ScienceOn
8 Goulhen, F., Gloter, A., Guyot, F. and Bruschi, M. (2006) Cr(VI) detoxification by Desulfovibrio vulgaris strain Hildenborough: microbe-metal interactions studies, Applied Microbiology and Biotechnology, v.71, p.892- 897.   DOI
9 Guertin, J., Jacobs, J.A. and Avakian, C.P. (2005) Chromium( VI) Handbook. CRC Press. Florida. p.2-19.
10 Horton, R.N., Apel, W.A., Thompson, V.S. and Sheridan, P.P. (2006) Low temperature reduction of hexavalent chromium by a microbial enrichment consortium and a novel strain of Arthrobacte aurescens, BMC Microbiology, v.6(5), doi:10.1186/1471-2180-6-5   DOI
11 Hussein, H., Ibrahim, S.F., Kandeel, K. and Moawad, H. (2004) Biosorption of heavy metals from waste water using Pseudomanas sp., Electronic Journal of Biotechnology, v.7(1), p.30-37.
12 Iyer, A., Mody, K. and Jha, B. (2004) Accumulation of hexavalent chromium by an exopolysaccharide producing marine Enterobacter cloaceae, Marine Pollution Bulletin, v.49, p.974-977.   DOI
13 Kim, P.G. (2004) Geotechnical characteristics of regional area in Korea: Deajeon-Chungnam area. Seminar and Meeting Report of Korean Geotechnical Scoiety, p.158-164.
14 Lee, J.-U., Chon, H.-T. and John, Y.W. (1997) Geochemical characteristics of groundwater with different geology an temperature-Comparative study with granitic groundwater, Journal of the Korean Society of Groundwater Environment, v.4(4), p.212-222.   과학기술학회마을
15 Lee, S.-E., Lee, J.-U., Lee, J.-S. and Chon, H.-T. (2005) Cr (VI) reduction by indigenous bacteria in anaerobic sediment, J. of Mineral and Energy Resources, v.42(6), p.618-624.
16 Llovera, S., Bonet, R., Simon-Pujol, M.D. and Congregado, F. (1993) Chromate reduction by resting cells of Agrobacterium radiobacter EPS-916, Applied and Environmental Microbiology, v.59(11), p.3516-3518.
17 Lovley, D.R., Widman, P.K., Woodward, J.C. and Phillips, E.J.P. (1993) Reduction of uranium by cytochrome c3 of Desulfovibrio vulgaris, Applied and Environmental Microbiology, v.59(11), p.3572-3576.
18 McLean, J. and Beveridge, T.J. (2000) Chromate reduction by a Pseudomonad isolated from a site contaminated with chromated copper arsenate, Applied and Environmental Microbiology, v.67(3), p.1076-1084.
19 Lowe, K.L., Straube, W.B. and Jones-Meehan, J. (2003) Aerobic and anaerobic reduction of Cr(VI) by Shewanella oneidensis effects of cationic metals, sorbing agents and mixed microbial culture, Acta Biotechnology, v.23(2-3), p.161-178.   DOI
20 Madigan, M.T. and Martinko, J.M. (2006) Brock Biology of Microorganisms 11th, Prentice Hall. Pearson Education Korea LTD, and World Science, Seoul, Korea, p.108-126.
21 Ministry of Land and Transport Affairs, K-Water, Korea Institute of Geoscience and Mineral Resources, 2006, 2006 research report on area of contaminated groundwater and countmeasure plan, p.249-310.
22 Moon, H.J. (2007) Policies for the Rational Utilization and Management of Groundwater Resources. Korea Environment Institute, p.5-17.
23 Ohtake, H., Wang, P.C., Mori, T., Komori, K., Sasatsu, M. and Toda, K. (1989) Isolation and chracterization of an Enterobacter cloacae strain that ruduces hexavalent chromium under anaerobic conditions, Applied and Environmental Microbiology, v.55, p.1665-1669.
24 Palmer, C.D. and Puls, R.W. (1994) EPA Ground Water Issue EPA/540/5-94/505
25 Palmer, C.D. and Wittbrodt, P.R. (1991) Processes affecting the remediation of chromium-contaminated sites, Environmental Health Perspectives, v.92, p.25-40.   DOI   ScienceOn
26 Pinon-Castillo, H.A., Brito, E.M.S., Goni-Urriza, M., Guyoneaud, R., Duran, R., Nevarez-Moorillon, G.V., Gutierrez-Corona, J.F., Carretta, C.A. and Reyna- Lopez, G.E. (2010) Hexavalent chromium reduction by bacterial consortia and pure strains from an alkaline industrial effluent, J. of Applied Microbiology, v.109, p.2173-2182.   DOI
27 Remoundaki, E., Hatzikioseyian, A. and Tsezos, M. (2007) A systematic study of chromium solubility in the presence of organic matter: consequences for the treatment of chromium-containing wastewater, J. of Chemical Technology and Biotechnology, v.82, p.802-808.   DOI
28 Puzon, G.J., Roberts, A.G., Kramer, D.M. and Xun, L. (2005) Formation of soluble organo-Chromium(III) complexes after chromate reduction in the presence of cellular organics, Environmental Science & Technology, v.39, p.2811-2817.   DOI
29 Puzon, G.J., Huang, Y., Dohnalkova, A. and Xun, L. (2008) Isolation and characterization of an $NAD^+$- degrading bacterium PTX1 and its role in chromium biogeochemical cycle, Biodegradation, v.19, p.417-424.   DOI
30 Rai, D., Sass, B.M. and Moore, D.A. (1987) Chromium( III) hydrolysis constants and solubility of chromium( III) hydroxide, Inorganic Chemistry, v.26, p.345-349.   DOI
31 Rhee, S.-K. (2007) Analyses of microbial diversity of contaminated groundwater of Moonpyung, Daejeon based on microbial ecology. Consulting report
32 Seo, J.K. and Kim, M.K. (2006) The characteristics of groundwater quality with different aquifer geology in the Samnam Area, Ogcheon, J. of Mineral and Energy Resources, v.43(4), p.359-368.
33 Sharma, K. (2002) Microbial Cr(VI) reduction: Role of Electron Donors, Acceptors, and Mechanisms, with Special Emphasis on Clostridium spp., Ph D. thesis, University of Florida, USA, 36p.
34 Shen, H. and Wang, Y.T. (1993) Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456, Applied and Environmental Microbiology, v.59(11), p.3771-3777.
35 Smith, W.L. and Gadd, G.M. (2000) Reduction and precipitation of chromate by mixed culture sulphatereducing bacterial biofilms, J. of Applied Microbiology, v.88, p.983-991.   DOI
36 Wang, Y.-T. and Xiao, C. (1995) Factors affecting hexavalent chromium reduction in pure cultures of bacteria, Water Research, v.29, p.2467-2474.   DOI   ScienceOn
37 Swayambunathan, V., Liao, Y.X. and Meisel, D. (1989) Stages in the evolution of colloidal chromium(III) oxide, Langmuir, v.5, p.1423-1427.   DOI
38 Tsezos, M., Hatzikioseyian, A. and Remoundaki, E. (2007) A systematic study of chromium solubility in the presence of organic matter: consequences for the treatment of chromium-containing wastewater, J. of Chemical Technology and Biotechnology, v.82, p.802-808.   DOI
39 Turick, C.E. and Apel, W.W. (1997) Method for in situ or ex situ bioremediation of hexavalent chromium contaminated soils and/or ground water, US Patent Issued
40 Zhu, W., Yang, Z., Ma, Z. and Chai, L. (2008) Reduction of high concentration of chromate by Leucobacter sp. CRB1 isolated from Changsha, China, World Journal of Microbiology and Biotechnology, v.24, p.991-996.   DOI
41 Berkeley Lab. (2003) Bioremediation of metals and radionuclides, 2nd edition. p.9-15.
42 Ackerley, D.F., Gonzalez, C.F., Keyhan, M., Blake, R. and Martin, A. (2004) Mechanism of chromate reduction by the Escherichia coli protein, NfsA, and the role of different chromate reductases in minimizing oxidative stress during chromate reduction, Environmental Microbiology, v.6(8), p.851-860.   DOI
43 Appukuttan, D., Rao, A.S. and Apte, S.K. (2006) Engineering of Deinococcus radiodurans R1 for bioprecipitation of uranium from dilute nuclear waste, Applied and Environmental Microbiology, v.72(12), p.7873-7878.   DOI
44 Bartlett, R.J. (1991) Chromium cycling in soils and water: links, gaps, and methods, Environmental Health Perspectives, v.92, p.17-24.   DOI
45 Cheung, K.H. and Gu, J.D. (2005) Chromate reduction by Bacillus megaterium TKW3 isolated from marine sediments, World Journal of Microbiology & Biotechnology, v.21, p.213-219.   DOI