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http://dx.doi.org/10.9719/EEG.2012.45.2.105

Biogeochemical Remediation of Cr(VI)-Contaminated Groundwater using MMPH-0 (Enterobacter aerogenes)  

Seo, Hyun-Hee (Department of Earth and Environmental Sciences, Chonnam National University)
Rhee, Sung-Keun (Department of Microbiology, Chungbuk National University)
Kim, Kang-Joo (Department of Environmental Engineering, Kunsan National University)
Park, Eun-Gyu (Department of Geology, Kyungpook National University)
Kim, Yeong-Kyoo (Department of Geology, Kyungpook National University)
Chon, Chul-Min (Geologic Environment Division, Korea Institute of Geosciences and Mineral Resources)
Moon, Ji-Won (Biosciences Division, Oak Ridge National Laboratory)
Roh, Yul (Department of Earth and Environmental Sciences, Chonnam National University)
Publication Information
Economic and Environmental Geology / v.45, no.2, 2012 , pp. 105-119 More about this Journal
Abstract
Indigenous bacteria isolated from contaminated sites play important roles to remediate contaminated groundwater. Chromium has the most stable oxidation states. Cr(VI) is toxic, carcinogenic, and mobile, but Cr(III) is less toxic and immobile. In this study, indigenous microorganism (MMPH-0) was enriched from Cr(VI) contaminated groundwater, and identified by 16S rRNA gene analysis. Using MMPH-0, the effect of stimulating with e-donors (glucose, lactate, acetate, and no e-donor control), respiration conditions, biomass, tolerance, and geochemical changes on Cr(VI) reduction were investigated in batch experiments for 4 weeks. The changes of Cr(VI) concentration and geochemical conditions were monitored using UV-vis-spectrophotometer and Eh-pH meter. And the morphological and chemical characteristics of MMPH-0 and precipitates in the effluents were characterized by TEM-EDS and SEM-EDS analyses. MMPH-0 (Enterobacter aerogenes) was able to tolerate up to 2000 mg/L Cr(VI) and reduce Cr(VI) under aerobic and anaerobic conditions. MMPH-0 performed faster and higher efficiency of Cr(VI) reduction with electron donors (over 70% after 1 week with e-donor, 10-20% after 4 weeks without e-donor). The changes of Eh-pH in effluents showing the tendency from oxidizing to reducing condition and a bit of acidic change in pH due to microbial oxidation of organic matters donating electrons and protons suggested the roles of MMPH-0 on Cr(VI) in the contaminated water catalyzing to transit geochemical stable zone for more stable $Cr(OH)_3$ or Cr(III) precipitates. TEM/SEM-EDS analyses of MMPH-0 and precipitates indicate direct and indirect Cr(VI) reduction: extracellular polymers capturing Cr component outside cells. These results suggested diverse indigenous bacteria and their biogeochemical reactions might enhance more effective and feasible remediation technology of redox sensitive heavy metals in metal-contaminated in groundwater.
Keywords
indigenous bacteria; Enterobacter aerogenes; Cr(VI); biogeochemistry; reduction-oxidation reaction;
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