Gametogenesis, changes of gonad bulk index (GBI), monthly variation in oocyte diameter, reproductive cycle and spawning with lunar cycle of the top shell, Trochus niloticus were investigated by histological examination. The specimens were sampled in the coastal waters of Chuuk Island, Micronesia, form November 1999 to September 2000. T. niloticus is a gonochorism, and the female and male were present in an approximately 1:1 ratio (p > 0.05). The ovary contained with the oocyte of yolk stage, the testis composed of the spermatid and spermatozoa at around the year. Monthly GBI were higher at February, March, April and June than the other months. Major spawning occurred between April and May, and June and July but the individuals of partially spawning were presented throughout the year. GBI with lunar cycle were higher at the full moon than the other lunar phase. These results suggest that the spawning occurred between the full moon and last quarter moon.
Park, Chang-Beom;Kim, Byung-Ho;Na, Oh-Soo;Choi, Young-Chan;Lee, Young-Don;Baek, Hae-Ja;Kim, Hyung-Bae;Akihiro Takemura
Animal cells and systems
/
v.7
no.3
/
pp.227-235
/
2003
Bisphenol A (BPA), nonylphenol (NP), and 4-tert-octylphenol (OP) are known endocrine disrupting chemicals (EDCs) with estrogenic activity in fish. This study compared the effects of BPA, NP and OP on in vitro vitellogenin (VTG) synthesis in primary cultures of hepatocytes of the Chinese minnow Phoxinus oxycephalus. The VTG secreted into the culture medium was measured using enzyme-linked immunosorbent assay (ELISA), which we developed in this study using an antibody prepared from homogenates of Chinese minnow egg. VTG synthesis was induced by estradiol-17$\beta$ ($E_2$) and phenols (BPA, NP and OP) treatment. $E_2$ at concentrations of 10$^{-6}$ M or higher increased VTG levels significantly (P < 0.05). Exposure to 10^5\;M\;BPA\;or\;10^-4$M NP and OPinduced in vitro VTG synthesis (P < 0.01). However, $10^-3$ M BPA, NP or OP did not induce VTG synthesis. These results suggest that SPA has the highest estrogenic potential in Chinese minnow hepatocytes. Tamoxifen, an anti-estrogen, drastically blocked the production of VTG by phenols (BPA, NP and OP) suggesting that phenols (BPA, NP and OP) may act via binding to estrogen receptor (ER) in Chinese minnow hepatocytes.
The global terrestrial ecosystems have shown a large spatial variability in recent decades and represented a carbon sink pattern at mid-to-high latitude in Northern Hemisphere. However, there are many uncertainties in magnitude and spatial distribution of terrestrial carbon fluxes due to the effect of climate factors. So, it needs to accurately understand the spatio-temporal variations on carbon exchange flux with climate. This study focused on the effects of climate factors, .i.e. temperature, precipitation, and solar radiation, to terrestrial biosphere carbon flux. We used the terrestrial carbon flux that is simulated by a CarbonTracker, which performs data assimilation of global atmospheric $CO_2$ mole fraction measurements. We demonstrated significant interactions between Net Ecosystem Production (NEP) and climate factors by using the partial correlation analysis. NEP showed positive correlation with temperature at mid-to-high latitude in Northern Hemisphere but showed negative correlation pattern at $0-30^{\circ}N$. Also, NEP represented mostly negative correlation with precipitation at $60^{\circ}S-30^{\circ}N$. Solar radiation affected NEP positively at all latitudes and percentage of positive correlation at tropical regions was relatively lower than other latitudes. Spring and summer warming had potentially positive effect on NEP in Northern Hemisphere. On the other hand as increasing the temperature in autumn, NEP was largely reduced in most northern terrestrial ecosystems. The NEP variability that depends on climate factors also differently represented with the type of vegetation. Especially in crop regions, land carbon sinks had positive correlation with temperature but showed negative correlation with precipitation.
Yun J. I.;Nam J. C.;Hong S. Y.;Kim J.;Kim K. S.;Chung U.;Chae N. Y.;Choi T. J
Korean Journal of Agricultural and Forest Meteorology
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v.6
no.3
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pp.149-163
/
2004
Evapotranspiration (ET) is a critical component of the hydrologic cycle which influences economic activities as well as the natural ecosystem. While there have been numerous studies on ET estimation for homogeneous areas using point measurements of meteorological variables, monitoring of spatial ET has not been possible at landscape - or watershed - scales. We propose a site-specific application of the land surface model, which is enabled by spatially interpolated input data at the desired resolution. Gyunggi Province of South Korea was divided into a regular grid of 10 million cells with 30m spacing and hourly temperature, humidity, wind, precipitation and solar irradiance were estimated for each grid cell by spatial interpolation of synoptic weather data. Topoclimatology models were used to accommodate effects of topography in a spatial interpolation procedure, including cold air drainage on nocturnal temperature and solar irradiance on daytime temperature. Satellite remote sensing data were used to classify the vegetation type of each grid cell, and corresponding spatial attributes including soil texture, canopy structure, and phenological features were identified. All data were fed into a standalone version of SiB2(Simple Biosphere Model 2) to simulate latent heat flux at each grid cell. A computer program was written for data management in the cell - based SiB2 operation such as extracting input data for SiB2 from grid matrices and recombining the output data back to the grid format. ET estimates at selected grid cells were validated against the actual measurement of latent heat fluxes by eddy covariance measurement. We applied this system to obtain the spatial ET of the study area on a continuous basis for the 2001-2003 period. The results showed a strong feasibility of using spatial - data driven land surface models for operational monitoring of regional ET.
To establish the method for investigating the behavior of aerosol particles deposited on the leaf surface against fog water under natural conditions, scanning electron microscopy with energy-dispersive X-ray (SEM-EDX) analysis and wash water analysis by ion chromatography after the washing treatment were performed using leaves of white birch collected from low part of the tree crown and the top of the tree in Sapporo City, Hokkaido, northern Japan. Each of collected leaves was divided into two parts according to the treatment performed: leaf surface (adaxial side) was 1) untreated, and 2) washed with deionized water with a pipette. In untreated samples, many particles of various shapes, including soil particles and organic debris, were deposited on the surface. Particles containing S were found on the surface of samples collected from only low part of the tree crown. After the washing treatment, SEM-EDX analysis revealed that soil particles and particles containing S had been washed off with water, although some particles such as soil particles and organic debris still remained on the leaf surface. The major anion such as $SO{_4}^{2-}$ was detected in wash water of all samples, although the peak of S in X-ray spectra was not detected from samples collected at top of the tree. The combination of SEM-EDX analysis with wash water analysis indicated that $SO{_4}^{2-}$ was deposited on the leaf surface in dissolved state and/or in state of submicron particles. These results suggested that fog water could remove soil particles and particles containing S and $SO{_4}^{2-}$ from the leaf surfaces, but not all particles. There was no difference in sampling position in the tree crown. Our study suggested that combination with SEM-EDX analysis and wash water analysis would be effective for investigation of the behavior of particles on the leaf surface against fog water.
Fog deposition onto the cool-temperate deciduous forest around Lake Mashu in northern Japan was estimated by the inferential method using the parameterizations of deposition velocity and liquid water content of fog (LWC). Two parameterizations of fog deposition velocity derived from field experiments in Europe and numerical simulations using a detailed multi-layer atmosphere-vegetation-soil model were tested. The empirical function between horizontal visibility (VIS) and LWC was applied to produce hourly LWC as an input data for the inferential method. Weekly mean LWC computed from VIS had a good correlation with LWC sampled by an active string-fog collector. By considering the enhancement of fog deposition due to the edge effect, fog deposition calculated by the inferential method using two parameterizations of deposition velocity agreed with that computed from throughfall data. The results indicated that the inferential method using the current parameterizations of deposition velocity and LWC can provide a rough estimation of water input due to fog deposition onto cool-temperature deciduous forests. Limitations of current parameterizations of deposition velocity related to wind speed, evaporation loss of rain and fog droplets intercepted by tree canopies, and leaf area index were discussed.
The fog water chemistry and deposition in northern Japan were investigated by fog water and throughfall measurements in 2010. Fog water was sampled weekly by an active-string fog sampler at Lake Mashu from May to November. Throughfall measurements were conducted using rain gauges under three deciduous trees along the somma of the lake from August to October. The mean fog deposition rate (flux) was calculated using throughfall data to estimate the total fog water deposition amount for the entire sampling period. $NH_4{^+}$ and $SO{_4}^{2-}$ were the most abundant cation and anion, respectively, in the fog water samples. A mean pH of 5.08 in the fog water, which is higher than those in rural areas in Japan, was observed. The [$NH_4{^+}$]/[$SO{_4}^{2-}$] equivalent ratio in fog water was larger than 1.0 throughout the study period, indicating that $NH_3$ gas was the primary neutralizing agent for fog water acidity. The mean rate and total amount of fog water deposition were estimated as 0.15 mm $h^{-1}$ and 164 mm, respectively. The amounts of nitrogen and sulfate deposition via fog water deposition were corresponded to those reported values of the annual deposition amounts via rainfall.
In this study, the Slab Ocean Model (SOM) is coupled with an Atmospheric General Circulation Model (AGCM) which developed in University of Kangnung based on the land surface model of Biosphere-Atmosphere Transfer Scheme (BATS). The purposes of this study are to understand radiative role of clouds considering of the atmospheric feedback, and to compare the Clouds Radiative Forcing (CRF) come from the analyses using the clear-cloud sky method and CGCM. The new CGCM was integrated by using two sets of the clouds with radiative role (EXP-A) and without radiative role (EXP-B). Clouds in this two cases show the negative effect $-26.0\;Wm^{-2}$ of difference of radiation budget at top of atmosphere (TOA). The annual global means radiation budget of this simulation at TOA is larger than the estimations ($-17.0 Wm^{-2}$) came from Earth Radiation Budget Experiment (ERBE). The work showed the surface negative effect with $-18.6 Wm^{-2}$ in the two different simulations of CRF. Otherwise, sensible heat flux in the simulation shows a great contribution with positive forcing of $+24.4 Wm^{-2}$. It is found that cooling effect to the surface temperature due to radiative role of clouds is about $7.5^{\circ}C$. From this study it could make an accurate of the different CRF estimation considering either feedback of EXP-B or not EXP-A under clear-sky and cloud-sky conditions respectively at TOA. This result clearly shows its difference of CRF $-11.1 Wm^{-2}$.
PCBs [Polychlorinated biphenyls] are halogenated aromatic compounds with the empirical formula C12H10-nCln(n=1~10), and are a mixture of possible 209 different chlorinated congeners. PCBs were widely used as dielectric fluids for capacitors, transformers, plasticizers, lubricant inks, and paint additives. once released into the environment, PCBs persist for years because they are so resistant to degradation. In addition to their high degree of lipophilicity. In 1970s, the worldwide production of PCBs seem to be still in use. The environmental load of PCBs was prohibited since 1983 in Korea. In spite of these actions, many PCBs seem to be still in use. The environmental load of PCBs will continue to be recycled through air, land, water, and the biosphere for decades to come. This study was conducted to measure the concentrations of PCBs I the serum samples of 112 women by GC/MSD(Hewlett Packard 5897 Gas Chromatography-Mass Chromatography Detector) and CG/ECD(Hewlett Packard 5890 series-II gas chromatography-Electron capture detector, U.S.A). The main results of this study were as follows; The mean and standard deviation of serum PCBs were 3.613, 0.759 ppb, respectively and median of it was 3.828 ppb. The correlation coefficients of the concentrations of 13 PCB congeners ranged from 0.7913 to 0..9985 and were significantly correlated between each items(p=0.0001). The PCB concentrations were positively associated with age(simple linear regression; R2=0.86, =0.08023, p<0.001) and with total lipids in serums(simple linear regression; R=0.7058, =0.00486, p<0.001). The age adjusted model (Y=$\beta$0+$\beta$1age+$\beta$2X) was applied for possible predictors of PCBs levels in serum. For BMI(Body Mass Index), major residential area, and fish, meat, and dairy consumption, there was no association with PCBs levels, Also there was negative association for the number of pregnancy and lactation period with PCBs levels.
Recently we have discovered that sediments should be separated from lithosphere, and soil should be separated from biosphere, both sediment and soil will be mixed sediments-soil-sphere (Seso-sphere), which is using particulate mechanics to be solved. Erosion and sediment both are moving by particulate matter with water or wind. But ancient sediments will be erosion same to soil. Nowadays, real soil has already reduced much more. Many places have only remained sediments that have ploughed artificial farming layer. Thus it means sediments-soil-sphere. This paper discusses sediments-soil-sphere erosion modeling. In fact sediments-soil-sphere erosion is including water erosion, wind erosion, melt-water erosion, gravitational water erosion, and mixed erosion. We have established geographical remote sensing information modeling (RSIM) for different erosion that was using remote sensing digital images with geographical ground truth water stations and meteorological observatories data by remote sensing digital images processing and geographical information system (GIS). All of those RSIM will be a geographical multidimensional gray non-linear equation using mathematics equation (non-dimension analysis) and mathematics statistics. The mixed erosion equation is more complex that is a geographical polynomial gray non-linear equation that must use time-space fuzzy condition equations to be solved. RSIM is digital image modeling that has separated physical factors and geographical parameters. There are a lot of geographical analogous criterions that are non-dimensional factor groups. The geographical RSIM could be automatic to change them analogous criterions to be fixed difference scale maps. For example, if smaller scale maps (1:1000 000) that then will be one or two analogous criterions and if larger scale map (1:10 000) that then will be four or five analogous criterions. And the geographical parameters that are including coefficient and indexes will change too with images. The geographical RSIM has higher precision more than mathematics modeling even mathematical equation or mathematical statistics modeling.
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