• Biomedical Science Letters
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• v.18 no.4
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• pp.329-337
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• 2012

${\alpha}$-synuclein (${\alpha}$-syn) is known to be implicated in the pathogenesis of Parkinson's disease and transiently bind to biological vesicles. In this study, we examined the effect of molecular crowding on the interaction of ${\alpha}$-syn with biological vesicles by using inert polymers since the environment of proteins in cells are crowded with other macromolecules. The addition of different polymers including polyethylene glycol, dextran, and ficoll enhanced the binding of ${\alpha}$-syn to vesicles in a concentration-dependent manner and the association of ${\alpha}$-syn with vesicle was proportionally augmented by increased expression of ${\alpha}$-syn. However, molecular crowding had a neglectable effect on the vesicle binding of ${\alpha}$-syn mutants (A30P, TG6), which has been reported to show impaired vesicle binding capacity. These results suggest that transient interaction of ${\alpha}$-syn with vesicles occurs more commonly in cells than expected implying interaction with vesicles may be one of the physiological processes in which ${\alpha}$-syn is involved.

• Journal of Sensor Science and Technology
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• v.14 no.4
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• pp.244-249
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• 2005

In this study, radiation sensor tips are fabricated for remote sensing of X or gamma ray with inorganic scintillators and plastic optical fiber. The visible range of light from the inorganic scintillator that is generated by radiation source is guided by the plastic optical fiber and is measured by optical detector and power-meter. Two kinds of sensor tips are designed and fabricated such as film type and powder type. Many kinds of inorganic scintillators are used to fabricate both sensor tips, and the different wavelength of emitting lights from them are measured to determine the optimal inorganic scintillator which has maximum light output. As a radiation source X-ray generator and Ir-192 are selected to test a performance of sensor tip. It is expected that the fiber-optic radiation sensor is widely used in nuclear industry and medical applications due to its special characteristics such as good flexibility, easy in processing, long lengths and no interference to electro magnetic field.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.05c
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• pp.79-82
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• 2003

a-Se film is known as promising medical X-ray detector material but a-Se as dopanted Tellurium is not available in X-ray detectors. a-Se thick film was grown by vacuum thermal evaporator to $3{\mu}m$ thickness. The characteristics of thick films were analyzed by XRD, U-V Meter, and SEM measurements. Te composition is 0.1, 0.3, 0.5, 0.7g. This paper is fundmental data for phosphor layer's essential parameter that selenium have absorption wavelength along to various Te concentration rate.

• Journal of Sensor Science and Technology
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• v.19 no.6
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• pp.490-496
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• 2010

In this study, in order to overcome the weakness of acoustic field analysis which is generally used for ultrasonic probe performance evaluation, automatic acoustic field measurement system and evaluation parameters were proposed. The comparisons between acoustic field simulation and measured acoustic distribution data of normal and abnormal channels were conducted to evaluate the availability of proposed system and evaluation parameters. First, the impulse response characteristic of sample probe was investigated to classify the normal elements and abnormal elements. And then, normal channels and abnormal channels with abnormal element were chosen. The suggested 12 evaluation parameters were calculated using the acoustic fields of these channels. The availability of proposed automatic acoustic field measurement system and evaluation parameters was confirmed. And the performance evaluation of ultrasonic probe using acoustic field analysis could be easier and faster.

• Clinical and Experimental Reproductive Medicine
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• v.39 no.2
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• pp.41-45
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• 2012

Transcription factors govern diverse aspects of cell growth and differentiation as major switches of gene expression. Etv5, a member of the E26 transformation-specific family of transcription factors, has many stories to share when it comes to reproduction. Etv5 deficient mice show complex infertility phenotypes both in males and females. In males, the infertility phenotype exhibited by Etv5 deficiency is sexually dimorphic, and it involves both somatic cells and germ cells. In $Etv5^{-/-}$ female mice, the problem is more complicated by hormonal involvement. This review synthesizes old and new information on this versatile transcription factor-from the inadvertent discovery of its role in the testes to its newly discovered role in maintaining spermatogonial stem cells.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.92-93
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• 1998

In this study, we have established the technique to estimate the gradient waveforms distorted by the eddy current in MRI. After obtaining the eddy current effect transfer function using the finite element method, we have used the transfer function to estimate the output gradient waveforms at any points inside the imaging region. We also present experimental results to be compared with estimated ones.

• Proceedings of the Optical Society of Korea Conference
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• 2008.07a
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• pp.159-160
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• 2008

We measured hemodynamic responses of seizure in the mouse brain using frequencydomain near infrared spectroscopy (NIRS) and electroencephalogram (EEG). We adapted microfabricated optical holder for consistent contact of the optical fiber to the mouse brain. Our results show that the cerebral oxygenation and hemodynamics of mice can be stably monitored with EEG in the mouse brain.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.530-531
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• 2001

Out of 20 primers, 6 generated 1349 highly reproducible RAPD markers, producing approximately 5.2 polymorphic bands per primer in catfish(Sizurus asotus) population from Kunsan. The electrophoretic analysis of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPD) products showed the middle levels of similarity between different individuals in population from Kunsan. That is to say, the degree of similarity varied from 0.40 to 0.54, with the average of 0.46, as calculated by bandsharing analysis. (omitted)

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.223.2-223.2
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• 2015

Polyurethane acrylate (PUA) has been introduced to utilize as a mold material for sub-100 nm lithography as it provides advantages of stiffness for nanostructure formation, short curing time, flexibility for large area replication and transparency for relevant biomedical applications. Due to the ability to fabricate nanostructures on PUA, there have been many efforts to mimic extracellular matrix (ECM) using PUA especially in a field of tissue engineering. It has been demonstrated that PUA is useful for investigating the nanoscale-topographical effects on cell behavior in vitro such as cell attachment, spreading on a substrate, proliferation, and stem cell fate with various types of nanostructures. In this study, we have conducted surface modification of PUA films with micro/nanostructures on their surfaces using plasma treatment. In general, it is widely known that the plasma treated surface increases cell attachment as well as adsorption of ECM materials such as fibronectin, collagen and gelatin. Effect of plasma treatment on PUA especially with surface of micro/nanostructures needs to be understood further for its biomedical applications. We have evaluated the modified PUA film as a culture platform using adipose derived stem cells. Then, the behavior of stem cells and the level of adsorbed protein have been analyzed.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.28 no.1
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• pp.1-8
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• 2018

The 23 wt% polyvinylidene fluoride (PVDF)/15 wt% polyurethane (PU) fibers were electrospun using the conjugated nozzle at a flow rate of 1.0 mL/h and an electric field of 1 kV/cm. The formation of ${\beta}$ crystal phase in the PVDF and the PVDF/PU fibers was confirmed by Fourier transform infrared spectroscopy. After electrospinning, the asspun fibers were immersed in a boiling water and then dried at $100^{\circ}C$ in a convection oven to make a crimp phenomenon. The crimps with a diameter of $2.0{\pm}0.08{\mu}m$ were observed for the PVDF/PU fibers after hydrothermal treatment without sacrificing the extent of ${\beta}$ crystal phase. All the PU, PVDF and PVDF/PU fibers exhibited average cell viability of more than 98 %. The cell proliferation results suggested that L-929 cells adhered well to the PU, PVDF and PVDF/PU fibers and proliferated continuously with increasing time, indicating that the PVDF/PU fibers are highly applicable to the biomedical applications.