• Journal of Biomedical Engineering Research
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• v.31 no.2
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• pp.87-93
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• 2010

Researches for stem cells have been focused on scientists in biomedical sciences as well as clinical application for its great therapeutic potentials. Stem cells have two distinct characteristics: self-renewal and differentiation. In this short review, the links between stem cell research and biomedical engineering is discussed based on the basic characteristics of stem cells. This concept can be extended to the fundamental questions of biological sciences for cells such as proliferation, apoptosis, differentiation, and migration. For understanding proliferation and apoptosis of stem cells, techniques from biomedical engineering such as surface patterning, MEMS, nanotechnologies have been used. The advanced technologies such as microfluidic technologies, three dimensional scaffold fabrication, and mechanical/electrical stimulation have also been used in cell differentiation and migration. Basic and unsolved questions in the stem cell research field have limitations by studying conventional technologies. Therefore, the strategic fusion between stem cell biology and novel biomedical engineering field will break the barriers for understanding fundamental questions of stem cells, which can open the window for the clinical applications of stem cell based therapeutics as well as regeneration of damaged tissues.

• Transactions on Electrical and Electronic Materials
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• v.5 no.4
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• pp.133-137
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• 2004

The development of digital x-ray detector has been extensively progressed for the application of various medical modalities. In this study, we introduce a new hybrid-type x-ray detector to improve problems of a conventional direct or indirect digital x-ray image technology, which composed of multi-layer structure using a CaWO$_4$ phosphor and amorphous selenium (a-Se) photoconductor. The leakage current of our detector was found to be ∼180 pA/cm$^2$ at 10 V/m, which was significantly reduced than that of a single a-Se detector. The x-ray sensitivity was measured as the value of 4230 pC/cm$^2$/mR at 10 V/m. We found that the parylene thin film between a CaWO$_4$ phosphor and an a-Se layer acts as an insulator to prevent charge injection from indium thin oxide (ITO) electrode into an a-Se layer under applied bias.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.51.1-51.1
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• 2011

An electrospun Poly (lactice-co-glycolide acid) (PLGA) and Gelatin nanofiber tube was fabricated for potential intestinal stent application. Mechanical properties of tube were evaluated by tensile strength and burst strength tests. Physical and chemical properties were evaluated by contact angle measurement, swelling rates and porosity measurements. Biodegradability was investigated by immersion in simulated body fluid (SBF). Biocompatibility was investigated in vitro by cytotoxicity and proliferation studies by MTT assay, confocal microscopy and western blot using IEC-18 (Rat intestinal epithelial cell). After intestinal stent was implanted into rat bowel for periods from 7 to 10days, it was then analyzed using micro-computed tomography (Micro CT) and X-ray techniques. Futhermore, histological analysis was performed by hematoxylin-eosin (H&E) stain.

• Journal of the Korean Society for Precision Engineering
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• v.31 no.12
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• pp.1067-1076
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• 2014

To date, biomedical application of three-dimensional (3D) printing technology remains one of the most important research topics and business targets. A wide range of approaches have been attempted using various 3D printing systems with general materials and specific biomaterials. In this review, we provide a brief overview of the biomedical applications using 3D printing techniques, such as surgical tool, medical device, prosthesis, and tissue engineering scaffold. Compared to the other applications of 3D printed products, the scaffold fabrication should be performed with careful selection of bio-functional materials. In particular, we describe how the biomaterials can be processed into 3D printed scaffold and applied to tissue engineering area.

• Biomedical Science Letters
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• v.9 no.3
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• pp.111-121
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• 2003

Recombinant single chain Fv (scFv) antibodies offer many advantages over mouse monoclonal antibodies such as faster clearance from blood, improved tumor localization, reduced human anti-mouse antibody (HAMA) response, and the availability to manipulate the scFv through genetic approaches. The recombinant phage display was constructed using lym-l hybridoma cells as a source of genetic starting material. mRNA was isolated from the corresponding antibodies hybridoma cells. VH and VL cDNA were amplified with RT-PCR and linked with ScFv by linker DNA to form ScFv DNA, which then were inserted into phagemid pCANTAB5E. The phage of positive clones selected with tube containing raji lymphoma cell and infected by competent E. coli HB2151 to express soluble scFv. The scFv lym-l was secreted into the cytosol and culture supernatant and shown to be of expected size (approximately 32 kDa) by western blot. An active scFv lym-l could be produced in E. coli with soluble form and high yield from hybridoma cell line, using phage display system. Immunoreactivity indicated that scFv lym1 showed a potential biding affinity against the raji lymphoma cell as its parental antibody (intact lym-l Ab).

• Journal of Microbiology and Biotechnology
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• v.21 no.11
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• pp.1193-1198
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• 2011

Encapsulation of biological material in the permiselective membrane allows to construct a system separating cells from their products, which may find biotechnological as well as biomedical applications in biological processes regulation. Application of a permiselective membrane allows avoiding an attack of the implanted microorganisms on the host. Our aim was to evaluate the performance of Bacillus subtilis encapsulated in an elaborate membrane system producing listeriolysin O, a cytolysin from Listeria monocytogenes, with chosen eukaryotic cells for future application in anticancer treatment. The system of encapsulating in membrane live Bacillus subtilis BR1-S secreting listeriolysin O was proven to exert the effective cytotoxic activity on eukaryotic cells. Interestingly, listeriolysin O showed selective cytotoxic activity on eukaryotic cells: more human leukemia Jurkat T cells were killed than human chronic lymphocytic B cells leukemia at similar conditions in vitro. This system of encapsulated B. subtilis, continuously releasing bacterial products, may affect selectively different types of cells and may have future application in local anticancer treatment.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.57-59
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• 1994

In this paper, we present the software part of the intelligent data processing unit (IDPU), which plays an important role in SiMACS. The software system processes ECG, EEG, EMG, blood pressure, respiration, temperature signals, and extracts some information about patient conditions. It displays the patient condition information and the signal data synchronously, and manages them together with other patient personal data in a network-based client/server environment. The software system is designed in an object-oriented paradigm, and implemented in C++ as a window-based application program.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.05
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• pp.184-187
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• 1997

In terms of a system identification, we present a method for autoregressive(AR) modelling of variious biomedical signal. Model order is estimated fly low rank approximation and coefficients are determined by innovation processes of Kalman filter derivation. An application of the method is given for visual evoked potentials.

• Journal of Biomedical Engineering Research
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• v.29 no.5
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• pp.364-370
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• 2008

Recent development in large area flat-panel x-ray detector technology enables clinical application of digital tomosyntesis. Unlike conventional motion tomography using x-ray films, flat-panel x-ray detectors provide projection images in digital formats so that tomographic images can be synthesized in a more flexible way. For the digital tomosynthesis, precise movements of the x-ray source and the x-ray detector with respect to a fulcrum point are necessary. In this study, we apply the digital tomosynthesis technique to the flat-panel detector based micro-CT in which the flat-panel detector and the x-ray source rotate together on a circular arc. The experimental results suggest that flat-panel detector based 3D CTs can be used for digital tomosynthesis in the clinical environment.

• Journal of Engineering Education Research
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• v.23 no.1
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• pp.59-64
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• 2020

The purpose of this study is to develop capstone design model of gender analysis methods suitable for engineering education field and examine improvements and effects by applying it to actual lessons for biomedical engineering students. Case study was performed to achieve the purpose of the study. Twelve gender analysis methods were applied to 'biomedical engineering capstone design' which was major course offered by department of biomedical engineering at J university. After the students understood how to analyze gender analysis methods and cases, they decided project topics and presented what gender analysis methods were applied for each project. Additionally, the results of analysis showed that the students were more able to understand the differences between men and women of all ages and try to narrow down the differences. They also found that they could contribute to development of new added value of knowledge and technology that reflected the needs of both men and women by applying gender analysis methods in system development.