• Journal of the Korean Society for Precision Engineering
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• v.30 no.2
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• pp.137-142
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• 2013

The indiscriminate use of the fossil fuel has caused serious environmental pollutions such as the shortage of energy and global warming. Microalgae have being emphasized as $3^{rd}$ generation biomass which makes the carbon dioxide reduce effectively as well as produces the biofuel. Large scale production of microbial biomass by continuous culture is a quite challenging issue, because off-line optimization strategies of a microbial process utilizing a model-based scheme give rise to many difficult problems. In this paper, the static and simple control method which was able to be applied in time-variant growth environment and large scale of algae culture was studied. The significant disturbances in on-line measurement of cell density were reduced by Savitzky-Golay FIR smoothing filter. Dunaliella salina was cultivated continuously in a flat panel photobioreactor by the on-off control of the turbidostat process.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.114.2-114.2
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• 2011

Furfural is a versatile derivative. It can be utilized for a building-block of furfuryl alcohol production and a component of fuels or liquid alkanes. But in bio-process, furfural is a critical compound because it inhibits cell growth and metabolism. Furfural could be converted from xylose and usually produced from biomass in which hemicellulose is abundant. In this study, furfural production from miscanthus was performed and utilization of miscanthus residue was consequently conducted. At first, hydrolysis for investigation of miscanthus composition and furfural production was performed using sulfuric acid. Previously, we optimized dilute acid pretreatment condition for miscanthus pretreatment and the condition was found to be about 15 min of reaction time, 1.5% of acid concentration and about $140^{\circ}C$ of temperature and 60% (about 7 g/L) of xylose was solubilized from miscanthus. Using the xylose, furfural production was conducted as second step. Approximately $160{\sim}200^{\circ}C$ of temperature was accompanied with the hydrolysis for pyrolysis of biomass. When the investigated condition; $180^{\circ}C$ of temperature, 20 min of reaction time and 2% of acid concentration was operated for furfural production, furfural productivity was reached to be 77% of theoretical maximum. After reaction, residue of miscanthus was utilized as feedstock of ethanol fermentation. Residue was well washed using water and saccharified using hydrolysis enzymes. Hydrolysate (glucose) from saccharification was utilized for the carbon source of Saccharomyces cervisiae K35.

• Journal of Marine Bioscience and Biotechnology
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• v.6 no.2
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• pp.118-122
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• 2014

The ocean provide great benefits for microalgal mass cultures with maintaining stable temperature due to high specific heat, mixing by wave energy, and providing large area for large-scale microalgae cultures. In this study, we cultivated a marine green microalga, Tetraselmis sp. KCTC12432BP, using marine photobioreactors on the ocean for investigating the effect of $NaHCO_3$ concentration on the biomass productivities and evaluating the potential of ocean microalgae culture. The culture medium consist of three fold concentrated f/2-Si with 4 g/L of $NaHCO_3$, which is dissolved in natural seawater. After 11 days of cultivation, the cultures reached stationary phase at biomass concentration of 1.6 g/L. At that time, $NaHCO_3$ concentration of 0, 2, and 4 g/L were fed to the cultures. The daily productivities of 0.11, 0.19, 0.30 g/L/day were attained with feeding rate of 0, 2, and 4 g/L $NaHCO_3$, respectively. Biomass productivity of Tetraselmis sp. KCTC12432BP was a function of the $NaHCO_3$ feeding rate as expected. This research shows that the microalgae can grow with $NaHCO_3$ as carbon source in marine photobioreactors on the ocean while exploiting various benefits of ocean cultivation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.5
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• pp.33-46
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• 2023

In this study, carbon storage in the aboveground biomass, litter layer, and soil layer was calculated for abandoned mining restoration areas to determine the level of carbon storage after the restoration project through comparison with the ecological reference. Five survey sites were selected for each abandoned mining restoration area in Boryeong-si, Chungcheongnam-do, and the ecological reference that can be a goal and model for the restoration project. The carbon storage in the restoration area was 0~21.3Mg C ha^-1, the deciduous layer 3.3~6.0Mg C ha^-1, and the soil layer(0-30cm) 8.3~35.1Mg C ha^-1, showing a significant difference in carbon storage by target site. The total carbon storage was between 6.1 and 35.3% of the ecological reference, with restoration area ranging from 14.0 to 62.4 Mg C ha^-1. The total carbon storage in the restoration area and the ecological reference differed the most in the aboveground biomass and was less than 12%. Based on these results, forest restoration area need to improve the carbon storage of forests through continuous management and monitoring so trees can grow and restore productivity in the early stages of the restoration project. The results of this study can be used as primary data for preparing future forest restoration indicators by identifying the storage of abandoned mining restoration areas.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.1
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• pp.15-26
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• 2008

The NEMURO model is aimed to efficiently understand the interaction among factors of lower trophic level of a marine ecosystem, using data on solar radiation and sea water temperature. In this study, we analyzed the seasonal pattern of nutrients and planktons, and estimated productivity and biomass of planktons from 2002 to 2005. Nutrients($NO_3$, $NH_4$, and $Si(OH)_4$) which were used by phytoplankton showed a high concentration before the bloom of phytoplankton. Nutrients (DON, PON, and Opal) which were a byproduct of phytoplankton showed a high concentration in the same period as the bloom of phytoplankton. Both phytoplankton and zooplankton had two peaks in March and August. Estimated phytoplankton biomass from the NEMURO model showed a similar pattern with observed chlorophyll a concentrations. Biomasses of phytoplankton were bigger than those of zooplankton. Annual mean biomasses of small and large phytoplankton were estimated at 30.961 and $14.070\;{\mu}g\;l^{-1}$ respectively. Annual mean biomass of predatory zooplankton was greater than those of small and large zooplankton.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.24 no.4
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• pp.548-561
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• 2019

The surfgrass Phyllospadix japonicus is a dominant seagrass species playing critical ecological roles on the eastern coast of Korea. However, few studies have been conducted on the ecological characteristics of this species, generally due to the turbulent water conditions in its habitat. In this study, to examine the growth dynamics of P. japonicus, we investigated monthly changes in morphological characteristics, density, biomass, and leaf productivity as well as changes in the underwater irradiance, water temperature, and water column nutrient concentrations of its habitat from August 2017 to July 2018. Underwater irradiance and water temperature showed clear seasonal changes increasing in spring and summer and decreasing in fall and winter. Nutrient availability fluctuated substantially, but did not display any distinct seasonal trend. Morphological characteristics, shoot density, biomass, and leaf productivities of P. japonicus exhibited significant seasonal variations, increasing in spring and decreasing in fall months. Spadix of P. japonicus occurred from March to August, with the maximum spadix percentage(15.8%) occurred in May 2018. The average leaf productivity of P. japonicus per shoot and area were 2.1 mg sht^-1 d^-1 and 7.5 g m^-2 d^-1, respectively. The optimum water temperature for the growth of P. japonicus in this study was between 13-14℃. The productivity of P. japonicus was not correlated with underwater irradiance, water temperature and nutrient concentrations. These results suggest that the study site provide sufficient amount of underwater irradiance, suitable water temperature range and nutrients for the growth of P. japonicus.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.1
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• pp.4-11
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• 2007

There have been increasing concerns about decreasing crop productivity due to salt accumulation in greenhouse soils. The objective of the study was to investigate the impact of rice straw and woodchip application to a salt accumulated greenhouse soil on crop productivity and soil quality. The application of rice straw (RS) and woodchip (W) increased tomato yield and decreased blossom-end rot, and increased yield of Chinese cabbage compared with standard recommended fertilization ($204-103-122kg\;ha^{-1}\;N-P_2O_5-K_2O$ for tomato and $222-64-110kg\;ha^{-1}\;N-P_2O_5-K_2O$ for Chinese cabbage), while less soil residual nitrate, phosphatephosphorus, and potassium. In addition to the organic material application, fertilization reduction based on soil testing may also contribute to relatively low level of soil residual nutrients. Application of the organic material reduced soil bulk density presumably because of improved soil aggregation and structure, and increased biomass C and dehydrogenase activity. In comparison to rice straw, woodchip application resulted in higher crop yield, less amount of soil residual nitrate and lower soil EC, and greater biomass and dehydrogenase activity. The results obtained in this study indicateshowed that woodchip amendment along with reduced fertilization based on soil testing can be one of essential management practices for salt accumulated greenhouse soils.

• Journal of Microbiology and Biotechnology
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• v.11 no.4
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• pp.644-648
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• 2001

The biological control potential of entomopathogenic nematodes (EPN) can be enhanced by improved culture efficiency. Optimization of the media is a key factor for improving in vitro mass production of entomopathogenic nematodes. This study reports the effect of medium concentration. The medium is a combination of carbohydrates, lipids, proteins, sats, and growth factors, on the growth of Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus liminescens. The overall optimal medium concentration for nematode recovery, hermaphrodite size, bacterial mass, infective juveniles (IJs) yield, and doubling time was 84 g/l. At this concentration rate, the doubling time of IJs production and the biomass of symbiotic bacteria was 1.6 days and 12.8 g/l, respectively. The maximum yield of $2.4{\times}{10^5}IJs/ml$ was attained within a one-generation cycle (eight days). The yield coefficient was $2.8{\times}{10^6}$ IJs/g medium, and the maximum productivity was $3.1{\times}{10^7}$ IJs per day. Medium concentration affected two independent factors, recovery and hermaphrodite size, which in turn influenced the final yield.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.487-493
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• 2013

The effects of long-term fertilization on soil biological properties and microbial community structure in the plough layer in a rice paddy soil in southern Korea were investigated in relation to the continuous application of chemical fertilizers (NPK), straw based compost (Compost), combination these two (NPK + Compost) for last 40 years. No fertilization plot (Control) was installed for comparison. Though fertilization significantly improved rice productivity over control, the long-term fertilization of NPK and compost combination was more effective on increasing rice productivity and soil nutrient status than single application of compost or chemical fertilizer. All fertilization treatments had shown significant improvement in soil microbial properties, however, continuous compost fertilization markedly increased soil enzyme and microbial activities as compared to sole chemical fertilization. Results of microbial community structure, evaluated by EL-FAME (ester-linked fatty acid methyl esters) method, revealed big difference among Control, NPK, and Compost. However, both Compost and Compost+NPK treatments belonged to the same cluster after statistical analysis. The combined application of chemical fertilizer and organic amendments could be more rational strategy to improve soil nutrient status and promote soil microbial communities than the single chemical fertilizer or compost application.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1399-1409
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• 2019

Biodiesel is a renewable and environmentally friendly liquid biofuel for transportation. Insect is considered as a new valuable biomass to convert into biodiesel. In particular, BSF(Black Soldier Fly) containing high fat is a renewable source of biodiesel. Biodiesel drived BSF has high concentration of saturated fatty acid methyl ester and low concentration of polyunsaturted fatty acid methyl ester which makes it potentially an ideal substrate for producing excellent quality biodiesel. Most of the fuel properties of BSF biodiesel were met the requirements of standard EN 14214. BSF have a higher lipid yield and biodiesel productivity as compared to microalgae and vegetable oils. This review paper includes the overall summary and compilation of the insect research conducted on biodiesel production and includes the BSF biodiesel properties.