• The Korean Journal of Ecology
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• 제25권5호
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• pp.315-320
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• 2002

본 연구는 어저귀(Abutilon avicennae)를 이용한 식물상 복원공법의 가능성과 카드뮴이 식물의 성장에 미치는 독성효과, 오염물과 식물유무에 따른 토양미생물의 활성변화, 카드뮴의 식물체내와 토양에서의 거동에 관한 연구를 실시하였다. 뿌리의 생체량은 90일 후 대조구에 비해 약 4배정도 저해를 받았으며, 지상부의 길이 생장 또한 대조구에 비해 약 3배정도 저해를 받은 것으로 나타났다. 식물내 카드뮴의 축적은 50일째 뿌리> 줄기> 잎의 순서로 축적을 나타냈으나, 90일이 경과된 후 카드뮴 축적은 뿌리>줄기>종자>잎으로 지상부 부분의 카드뮴이 잎에서 종자로 전이가 일어난 것을 볼 수 있다. 카드뮴 오염 유무에 상관없이 식물구는 무식물 구에 비해 미생물 활성도가 높게 나타났고 컬럼 하층부로 내려갈수록 감소하였다. 토양에서의 카드뮴 이동은 관찰되지 않았으며, 초기 가용성 카드뮴의 3.5%가 식물체내로 이동하였음이 관찰되었다.

• Journal of Ecology and Environment
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• 제31권3호
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• pp.167-176
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• 2008

Atmospheric N deposition has far-reaching impacts on forest ecosystems, including on-site impacts such as soil acidification, fertilization, and nutrient imbalances, and off-site environmental impacts such as nitrate leaching and nitrous oxide emission. Although chronic N deposition has been believed to lead to forest N saturation, recent evidence suggests that N retention capacity, particularly in the forest floor, can be surprisingly high even under high N deposition. This review aims to provide an overview of N retention processes in the forest floor and the implications of changing C-N interactions for C sequestration. The fate of available N in forest soils has been explained by the competitive balance between tree roots, soil heterotrophs, and nitrifiers. However, high rates of N retention have been observed in numerous N addition experiments without noticeable increases in tree growth and soil respiration. Alternative hypotheses have been proposed to explain the gap between the input and loss of N in N-enriched, C-limited systems, including abiotic immobilization and mycorrhizal assimilation, both of which do not require additional C sources to incorporate N in soil N pools. Different fates of N in the forest floor have different implications for C sequestration. N-induced tree growth can enhance C accumulation in tree biomass as observed across temperate regions. C loss from forests can amount to or outweigh C gain in N-saturated, declining forests, while another type of 'C-N decoupling' can have positive or neutral effects on soil C sequestration through hampered organic matter decomposition or abiotic N immobilization, respectively.

• Journal of Ecology and Environment
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• 제36권4호
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• pp.255-265
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• 2013

This study was conducted to determine the extent of drought resistance based on physiological responses of Calystegia soldanella under water deficit. In order to investigate the changes of plant growth, stomatal density, photosynthesis, chlorophyll fluorescence, the contents of chlorophyll and carotenoid, osmolality, total ion contents, the contents of carbohydrate and proline, C. soldanella was grown under well watered and drought stressed conditions for 12 days. In this study, water-deficit resulted in remarkable growth inhibition of C. soldanella. The effect of water-deficit on plant growth was associated with low osmotic potential of soil. On day 12 after drought treatment, dry weight, relative water contents, number and area of leaves and stem length were lower than those of control. The stomatal conductance and net photosynthetic rate were significantly reduced in water stressed plant to regulate inner water contents and $CO_2$ exchange through the stomatal pore. Chlorophyll fluorescence and chlorophyll contents were not different in comparison with the control, indicating that the efficiency of photosystem II was not affected by drought stress. This results could be explained that water-deficit in C. soldanella limits the photosynthetic rate and reduces the plant's ability to convert energy to biomass. A significant increase in total ion contents and osmolality was observed on day 7 and day 12. Accumulation of proline in leaves is associated with the osmotic adjustment in C. soldanella to soil water-deficit. Consequently, this increase in osmolality in water stressed plant can be a result in the increase of ion contents and proline.

• 한국토양비료학회지
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• 제47권6호
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• pp.398-405
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• 2014

Salt accumulation at soil surface is one of the most detrimental factors for crop production in reclaimed tidal land. This study was conducted to investigate the effect of capillary barriers beneath the soil surface on dynamics of soil salts at coarse-textured reclaimed tidal land. A field experiment was conducted at Saemangeum reclaimed tidal land for two years (2012-2013). Capillary barriers ($3.5{\times}12m$) were treated with crushed-stone, oyster shell waste, coal briquette ash, coal bottom ash, rice hull and woodchip at 40-60 cm depth from soil surface. Silage corn (Zea mays) was cultivated during the experimental period and soil salinity was monitored periodically. Soil salinity was significantly reduced with capillary barrier compared to that of control. Oyster shell waste was one of the most effective capillary barrier materials to control soil salinity at Saemangeum reclaimed tidal land. At the first growing season capillary barrier did not influence on corn growth regardless of types of the material, but plant biomass and withering rate of corn were significantly improved with capillary barrier at the second growing season. The results of this study showed that capillary barrier was effective on the control of soil salinity and improvement of corn growth, which indicated that capillary barrier treatment can be considered one of the best management practices for stable crop production at Saemangeum reclaimed tidal land.

• 한국환경농학회지
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• 제28권4호
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• pp.397-402
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• 2009

In Korea, salt stress is one of the major problems limiting crop production and eco-environmental quality in greenhouse soil. The objective of this study was to evaluate the effectiveness of organic residues (Chinese milk vetch, maize stalk, rice straw, and rye straw) for reducing salt activity in greenhouse soil. Organic residues was incorporated with salt-accumulated soil (EC, 3.0 dS $m^{-1}$) at the rate of 5% (wt $wt^{-1}$) and the changes of electrical conductivity (EC) was determined weekly for 8 weeks under incubation condition at $30^{\circ}C$. The EC, microbial biomass carbon (MBC), and water soluble ions in soil was strongly affected by C/N ratio of organic residues. After 8 weeks incubation, the concentration of water soluble $NO_3{^-},\;Ca^{2+}$, and $Mg^{2+}$ was significantly decreased in organic residues having high C/N ratio (maize stalk, rice straw, and rye straw) incorporated soil compared to organic residues having lower C/N ratio (Chinese milk vetch) incorporated soil. The EC value in Chinese milk vetch incorporated soil was higher than control treatment. In contrast, maize stalk, rice straw, and rye straw amended soil was highly decreased the EC value compared to control and Chinese milk vetch applied soil after 4 weeks incubation. Our results indicated that incorporation of organic residues having high C/N ratio (>30) could reduce salt activity resulting from reducing concentration of water soluble ions.

• BMB Reports
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• 제45권2호
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• pp.59-70
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• 2012

Carbon catabolite repression (CCR) is a key regulatory system found in most microorganisms that ensures preferential utilization of energy-efficient carbon sources. CCR helps microorganisms obtain a proper balance between their metabolic capacity and the maximum sugar uptake capability. It also constrains the deregulated utilization of a preferred cognate substrate, enabling microorganisms to survive and dominate in natural environments. On the other side of the same coin lies the tenacious bottleneck in microbial production of bioproducts that employs a combination of carbon sources in varied proportion, such as lignocellulose-derived sugar mixtures. Preferential sugar uptake combined with the transcriptional and/or enzymatic exclusion of less preferred sugars turns out one of the major barriers in increasing the yield and productivity of fermentation process. Accumulation of the unused substrate also complicates the downstream processes used to extract the desired product. To overcome this difficulty and to develop tailor-made strains for specific metabolic engineering goals, quantitative and systemic understanding of the molecular interaction map behind CCR is a prerequisite. Here we comparatively review the universal and strain-specific features of CCR circuitry and discuss the recent efforts in developing synthetic cell factories devoid of CCR particularly for lignocellulose-based biorefinery.

• 한국지하수토양환경학회지:지하수토양환경
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• 제8권4호
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• pp.12-20
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• 2003

본 연구에서는 원위치 생물학적 처리 과정에서 공대사 기작에 의해 분해되는 유기오염물질의 성상과 거동을 모의하기 위한 수학적 모델을 제기하였다. 토양구조 내에서 부동유역의 존재가 처리 과정에 미치는 영향을 고려하기 위하여 이중공극 개념을 적용하였으며, 유기오염물질의 거동과 생물학적 처리에 미치는 미생물의 영향을 수학적으로 표현하기 위하여 수정된 Monod식과 토양상 미생물의 미소군집모형이 적용되었다. 가상의 원위치 생물학적 처리 과정에 대한 모델의 적용을 통하여 공극내 생체축적으로 인한 투수능의 감소가 지하수 흐름에 미치는 영향이 예시되었다. 가상의 생물학적 처리 과정에 대한 모델의 모의결과는 부동유역의 존재가 유기오염물질의 생물학적 가용성을 저감시키며, 생물벽체의 형성 및 처리과정에 있어 외부로부터의 미생물 및 영양물질 주입정의 위치가 효과적인 처리 계획의 수립을 위해 중요하다는 것을 보여 주었다.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.44-53
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• 2020

Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 ㎍/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 10¹⁰ CFU/ml to 10¹² CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

• 한국환경과학회지
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• 제21권12호
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• pp.1435-1447
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• 2012

The present study was conducted to determine the effect of hexaconazole (HEX), a triazole fungicide, on the growth, yield, photosynthetic response and antioxidant potential in cucumber (Cucumis sativus L.) plants subjected to UV-B stress. UV-B radiation and HEX were applied separately or in combination to cucumber seedlings. The growth parameters were significantly reduced under UV-B treatment, however, this growth inhibition was less in HEX treated plants. HEX caused noticeable changes in plant morphology such as reduced shoot length and leaf area, and increased leaf thickness. HEX was quite persistent in inhibiting shoot growth by causing a reduction in shoot fresh and dry weight. HEX noticeably recovered the UV-B induced inhibition of biomass production. Significant accumutation in anthocyanin and flavonoid pigments in the leaves occurred as a result of HEX or UV-B treatments. HEX permitted the survival of more green leaf tissue preventing chlorophyll content reduction and higher quantum yield for photosystemII under UV-B exposure. HEX treatment induced a transient rise in ABA levels in the leaves, and combined application of HEX and UV-B showed a significant enhancement of ABA content which activates $H_2O_2$ generation. UV-B exposure induced accumulation of $H_2O_2$ in the leaves, while HEX prevented UV-B induced increase in $H_2O_2$, indicating that HEX serves as an antioxidant agent able to scavenge $H_2O$ to protect cells from oxidative damage. An increase in the ascorbic acid was observed in the HEX treated cucumber leaves affecting many enzyme activities by removing $H_2O_2$ during photosynthetic processes. The activities of antioxidant enzymes including catalase(CAT), ascorbate peroxidase(APX), superoxide dismutase(SOD) and peroxidase(POD) in the leaves in the presence of HEX under UV-B stress were higher than those under UV-B stress alone. These findings suggest that HEX may participate in the enhanced tolerance to oxidative stress. From these results it can be concluded that HEX moderately ameliolate the effect of UV-B stress in cucumber by improving the components of antioxidant defense system.

• 원예과학기술지
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• 제31권4호
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• pp.393-399
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• 2013

The effect of solution temperature and nitrogen form on cucumber (Cucumis sativus L.) growth, photosynthesis and nitrogen metabolism was investigated in hydroponic culture. Cucumber plants were grown for 35 days in a greenhouse at three constant solution temperatures ($15^{\circ}C$, $20^{\circ}C$, and $25^{\circ}C$) within a natural aerial temperature ($15-30^{\circ}C$). Four nitrate:ammonium ($NO{_3}^-:NH{_4}^+$) ratios (10:0, 8:2, 5:5, and 2:8 $mmol{\cdot}L^{-1}$) at constant nitrogen (N) concentration of $10mmol{\cdot}L^{-1}$ were applied within each solution temperature treatment. Results showed an increasing solution temperature enhanced plant growth (height, dry weight, and leaf area) in most N treatments. Dry weight accumulation was greatest at the 10:0 $NO{_3}^-:NH{_4}^+$ ratio in the $15^{\circ}C$ solution, the 5:5 ratio in the $20^{\circ}C$ solution and the 8:2 ratio in the $25^{\circ}C$ solution. Photosynthetic rate (Pn) response to solution temperature and $NO{_3}^-:NH{_4}^+$ ratio was similar to that of plant growth. Probably, the photosynthate shortage played a role in the reduced biomass formation. Increasing solution temperature enhanced the nitrate reductase (NR) activity, and further reduced shoots nitrate content. Our results indicate that the optimal ratio of nitrate to ammonium that promotes growth in hydroponic cucumber varies with solution temperature.