• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.904-909
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• 2005

To optimize the removal of free fatty acid in crude vegetable oil, response surface methodology was applied to determine the effects of five level-four factors and their reciprocal interactions on removal of free fatty acid. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, catalyst amount and methanol amount. A statistical model predicted that the highest removal yield of free fatty acid was 99.8%, at the following optimized reaction conditions: a reaction temperature of 64.99$^{\circ}C$, a reaction time of 36.20 mins., an catalyst amount of 13.01% (w/v), and a methanol amount of 15% (v/v). Using these optimal factor values under experimental conditions in three independent replicates, the average removal yield was well within the value predicted by the model.

• Proceedings of the Botanical Society of Korea Conference
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• 1995.06a
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• pp.79-90
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• 1995

Production of a cardiac glycoside, digoxin, by 12$\beta$-hydroxylation from digitoxin was studied in plant cell suspension cultures of Digitalis lanata. In order to increase the conversion yield, various culture conditions including immobilization were investigated and optimized. Since digoxin was released in the medium temporarily and converted further into a glucosylated product, deacetyllanatoside C, in situ adsorption of digoxin was employed to recover the product continuously. Amberlite resin XAD-8 showed the best adsorption characteristics for digoxin among the examined resins, and an integrated process was developed to increase the productivity. In addition, it was found that the utilization of $\beta$-cyclodextrin to entrap digoxin during the culture enhanced the biotransformation yield significantly.

• YAKHAK HOEJI
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• v.34 no.5
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• pp.296-301
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• 1990

Irradiation of the berberinephenolbetaine in a stream of argon produced the 8,14-cycloberberines [1]. On treatment with ethylchloroformate $C_8-N$ bond cleavage of the compound [1] occurred, accompanied with dehydrochlorination to give 7-ethylcarboxyisoquinoline [3], and the product [3] treated with strong alkali solution to give the 13-oxonorotensane [4] in 64% yield. Irradiation of the compound [4] converted easily to dihydro-8H-dibenzo[a, g] quinolizine-8-one [5]. and then the compound [5] was treated with methyliodide to give the 8-oxo-quinolizinium methiode. The intermediate colume chromatography on IRA-400 afforded the benzo[c, g]azecine-5-one[6] in 63% yield. The results of biological activities for these compounds are also presented.

• Journal of The Korean Society of Grassland and Forage Science
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• v.12 no.1
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• pp.12-18
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• 1992

This experiment was conducted to study the factors related with yield components in 5 varieties of perennial ryegrass and was surveyed for the biological yields(BY). shoot weight(SHW). root weight (RW), leaf weight(LW), stem weight(SW), plant length(PL), leaf area(LA), number of tillcr per plant (NT), root lcngth(RL), shoot and root ratio(S/R), ratio of root length and weight(RL/RW) and weight of a tiller(WT) in autumn growth stage. These results are summarized as follows;1. The yield components were varied during the stage of growth and in relation to the varieties in autumn. 2. The yield is consisted of 73 % by shoot and 27% by root in autumn.3. The varieties of the highest biological yields were Tempo, Maprima and the lowest biological yields were P-2, Caliente in autumn. 4. The variety of Tempo was largely affected by the factors of shoot weight, root weight, ratio of root length and weight, and weight of a tiller. but the variety of Caliente was influenced by the factors of leaf weight. stem weight, leaf area, SIR ratio and weight of a tiller on the yield. 5. The highest biological yields was obtained at the time of 4~5th(10/4~1018) and the lowest yield was showed at the time of 3~4th(9/30~1014) in autumn. 6. The time of the highest biological yields were resulted that the variety of Tempo was obtained between the time of 5~6th. Maprima was obtained between the time of 1~2nd and the varieties of Manhattan, P-2, Caliente were obtained between the time of 4~5th on the stages of growth in autumn.

• Korean Journal of Environmental Agriculture
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• v.42 no.1
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• pp.63-70
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• 2023

Golden apple snails (Pomacea canaliculat) (GAS) are widely used for weed control in rice cultivation. However, concerns on the ecological risk of invasive GAS species are increasing. This study aimed to evaluate the overall impacts of GAS on weed control, abundance of aquatic animals and insects, and rice yield, in comparison with that from other biocontrol agents such as loach (Misgurnus mizolepis) and catfish (Silurus asotus), which are alternatives for biological weed control in rice paddy cultivation. Field experiments included five treatments; control, herbicide, GAS, loach, and catfish. During the rice growth, weed appearance and biological abundance were monitored, and at harvest, the rice yield was determined. Weed control efficiency was the highest for GAS treatment (100%), followed by that for herbicide (95.8%), loach (57.5%), and catfish treatments (31.7%). Insect abundance was considerably decreased in GAS treatment due to heavy weed removal, which affects the habitat of aquatic animals and insects. The amount of rice yield (unit: kg 10 a^-1) was in the order of GAS (798.9) > loach (708.1) = herbicide (700.7) > catfish (629.4) > control (496.0). Therefore, considering the weed control efficiency, biological abudance, and rice yield, loach could be a potential alternative for biological weed control in organic rice farming. However, the activity of the biocontrol agents are susceptible to environmental conditions; therefore, further studies under different conditions are essential to confirm these findings.

• Journal of Microbiology and Biotechnology
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• v.17 no.7
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• pp.1183-1190
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• 2007

A metabolic uncoupler, 3,3',4',5-tetrachlorosalicylanilide (TCS), was used to reduce excess sludge production in biological wastewater treatment processes. Batch experiments confirmed that 0.4 mg/l of TCS reduced the aerobic growth yield of activated sludge by over 60%. However, the growth yield remained virtually constant even at the increased concentrations of TCS when cultivations were carried out under the anoxic condition. Reduction of sludge production yield was confirmed in a laboratory-scale anoxic-oxic process operated for 6 months. However, it was found that ammonia oxidation efficiency was reduced by as much as 77% in the presence of 0.8 mg/l of TCS in the batch culture. Similar results were also obtained through batch inhibition tests with activated sludges and by bioluminescence assays using a recombinant Nitrosomonas europaea (pMJ217). Because of this inhibitory effect of TCS on nitrification, the TCS-fed continuous system failed to remove ammonia in the influent. When TCS feeding was stopped, the nitrification yield of the process was resumed. Therefore, it seems to be necessary to assess the nitrogen content of wastewater if TCS is used for reducing sludge generation.

• The Korean Journal of Malacology
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• v.24 no.3
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• pp.189-197
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• 2008

In order to develop the sustainable ecological management, short-necked clams, Ruditapes philippinarum, were collected from Yoengi coast in Tongyoeng, Korea. The growth of the clam was estimated as: $L_f=68.08{\cdot}(1-e^{-0.145(t+0.324)})$ from ring radius composition of shell. Instantaneous co-efficiency of total mortality and natural mortality were calculated as: 0.991/year and 0.494/year, respectively. The age of the clams from the first capture was estimated to be 3.28. The total biomass was estimated to be 212 MT in the fisheries area (6.4 ha). Applied by these parameters, the annual recruit biomass and the current yield per recruit were calculated to be 649.5 individual/$m^2$ and $0.7\;g/m^2$, respectively. The current fishing intensity was much lower for maximum sustainable yield and acceptable biological catch. Although higher yield per recruit could be achieved by increasing fishing intensity, it is favorable to retain the current fishing intensity because of the unique fishing attitude on Yeongi coast in Tongyoeng, Korea.

• Magazine of the Korean Society of Agricultural Engineers
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• v.45 no.5
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• pp.85-96
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• 2003

The Revised Universal Soil Loss Equation (RUSLE) has been used in over 100 countries to estimate potential long-term soil erosion from the field. However, the RUSLE estimated soil erosion cannot be used to estimate the sediment delivered to the stream networks. For an effective erosion control, it is necessary to compute sediment delivery ratio (SDR) for watershed and sediment yield at watershed outlet. Thus, the Sediment Assessment Tool for Effective Erosion Control (SATEEC) was developed in this study to compute the sediment yield at any point in the watershed. To compute spatially distributed sediment yield map, the RUSLE was first integrated with the ArcView GIS and three area based sediment delivery ratio methods were incorporated in the SATEEC. The SATEEC was applied to the Bangdong watershed, Chuncheon, Gangwon Province to demonstrate how it can be used to estimate soil loss and sediment yield for a watershed. The sediment yield using USDA SDR method is 8,544 ton/year and 4,949 ton/year with the method by Boyce. Thus, use of watershed specific SDR is highly recommended when comparing the estimated sediment yield with the measured sediment data. The SATEEC was applied with hypothetical cropping scenario and it was found that the SATEEC can be used to assess the impacts of different management on the sediment delivered to the stream networks and to find the sediment source areas for a reach of interest. The SATEEC is an efficient tool to find the best erosion control practices with its easy-to-use interface.

• KSBB Journal
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• v.23 no.4
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• pp.335-339
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• 2008

Biodiesel is an alternative and renewable energy source, which is hoped to reduce global dependence on petroleum and environmental problem. Biodiesel produced from a variety of oil sources such as vegetable oil, animal fat and waste oils, and has properties similar to those associated with petro-diesel, including cetane number, volumetric heating value, flash point, viscosity and so on. In this study, we investigate the effect of quality of raw materials on alkali-catalyzed transesterification for producing of biodiesel. The increase of content of free fatty acid and water in oil were caused the sharp decrease of conversion yield. Also, the low purity of methanol in reactant was inhibited the reaction rate. In the case of addition of sodium sulfate as absorbent to prepare catalyst solution, the content of fatty acid methyl ester in product was increased more about 1.6% than that of control. However, the addition of zeolite, sodium chloride and sodium sulfate as absorbent in reactant to remove water generated from reaction did not show any enhancement in the reaction yield. This result may provide useful information with regard to the choice and preparation of raw materials for more economic and efficient biodiesel production.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.107-120
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• 2011

The ability to feed an expanding world population poses one of the greatest challenges to mankind in the future. Accompanying the increased demand for food by the expected nine billion inhabitants of Earth in 2050 will be a continual decrease in arable land area, together with a decline in crop yield due to a variety of stresses. For these formidable challenges to be met, future crops should not only by high-yielding, but also stress-tolerant and disease-resistant. In this review, we highlight the importance of genetic engineering as an indispensable tool to generate just such future crops. We briefly discuss strategies and available tools for biotechnological crop improvement and identify selected examples of candidate genes that may be manipulated so that current biological maxima in yield may be surpassed by comfortable margins. Future prospects and the necessity for basic research aimed at identifying novel target genes are also discussed.