• Title/Summary/Keyword: Biological variable

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A Review on the Measurement Variables of Nursing Research for Patients with Head and Neck Cancer in Korea (국내 두경부암환자를 대상으로 한 간호연구 측정변수에 대한 고찰)

  • Lee, Soon Neum
    • Journal of Korean Biological Nursing Science
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    • v.21 no.3
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    • pp.161-168
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    • 2019
  • Purpose: The purpose of this paper (a literature review study) was to confirm the trend of nursing research for head and neck cancer patients in Korea. Methods: Research databases were reviewed and analyzed from 13 papers (2004 through 2019 using KISS, NDSL, RISS, DBpia, and the National Assembly Library. As a result of this paper, we found that there were many studies that used questionnaires. Results: Measurement variables related to head and neck cancer patients were physical variables related to oral condition, psychological variables related to depression and anxiety, social support, family support related to family, and quality of life as a result variable. Conclusion: Therefore, integrated nursing intervention strategies and clinical nursing research considering the physical, psychological, social, and family aspects of head and neck cancer patients are needed. Based on the results of this study, we propose qualitative research on head and neck cancer patients, development of educational programs, intervention studies to verify effects, and development of clinical practice guidelines.

ITS Primers with Enhanced Specificity to Detect the Ectomycorrhizal Fungi in the Roots of Wood Plants

  • Kim, Dong-Hun;Chung, Hung-Chae;Ohga, Shoji;Lee, Sang-Sun
    • Mycobiology
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    • v.31 no.1
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    • pp.23-31
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    • 2003
  • With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal(ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.

Plant cell culture strategies for the production of natural products

  • Ochoa-Villarreal, Marisol;Howat, Susan;Hong, SunMi;Jang, Mi Ok;Jin, Young-Woo;Lee, Eun-Kyong;Loake, Gary J.
    • BMB Reports
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    • v.49 no.3
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    • pp.149-158
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    • 2016
  • Plants have evolved a vast chemical cornucopia to support their sessile lifestyles. Man has exploited this natural resource since Neolithic times and currently plant-derived chemicals are exploited for a myriad of applications. However, plant sources of most high-value natural products (NPs) are not domesticated and therefore their production cannot be undertaken on an agricultural scale. Further, these plant species are often slow growing, their populations limiting, the concentration of the target molecule highly variable and routinely present at extremely low concentrations. Plant cell and organ culture constitutes a sustainable, controllable and environmentally friendly tool for the industrial production of plant NPs. Further, advances in cell line selection, biotransformation, product secretion, cell permeabilisation, extraction and scale-up, among others, are driving increases in plant NP yields. However, there remain significant obstacles to the commercial synthesis of high-value chemicals from these sources. The relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.

Enrichment of Hydrogenotrophic Methanogens in Coupling with Methane Production Using Electrochemical Bioreactor

  • Jeon, Bo-Young;Kim, Sung-Yong;Park, Yong-Keun;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1665-1671
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    • 2009
  • Anaerobic digestion sludge was cultivated in an electrochemical bioreactor (ECB) to enrich the hydrogenotrophic methanogens. A modified graphite felt cathode with neutral red (NR-cathode) was charged with electrochemical reducing power generated from a solar cell. The methane and carbon dioxide collected in a Teflon bag from the ECB were more than 80 ml/l of reactant/day and less than 20 ml/l of reactant/day, respectively, whereas the methane and carbon dioxide collected from a conventional bioreactor (CB) was around 40 ml/l of reactant/day, respectively. Moreover, the maximal volume ratios of methane to carbon dioxide (M/C ratio) collected in the Teflon bag from the ECB and CB were 7 and 1, respectively. The most predominant methanogens isolated from the CB on the $20^{th}$, $80^{th}$, and $150^{th}$ days of incubation were hydrogenotrophs. The methanogenic diversity analyzed by temperature gradient gel electrophoresis (TGGE) of the 16S rDNA variable region was higher in the ECB than in the CB. The DNA extracted from the TGGE bands was more than 95% homologous with hydrogenotrophic methanogens in the ECB, but was an aceticlastic methanogen in the CB. In conclusion, the ECB was demonstrated as a useful system for enriching hydrogenotrophic methanogens and increasing the M/C ratio of the gas product.

Morphological Redescriptions of Three Condylostoma Ciliates (Heterotrichida: Condylostomatidae) New to Korea

  • Kim, Ji-Hye;Jang, Seok-Won;Shin, Mann-Kyoon
    • Animal Systematics, Evolution and Diversity
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    • v.28 no.3
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    • pp.149-160
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    • 2012
  • Heterotrichous ciliates were isolated from marine and brackish habitats in Korea, and their morphology, infraciliature and morphometrical characters were investigated using silver impregnated methods. These three Condylostoma species were identified as Condylostoma curva Burkovsky, 1970, C. minutum Bullington, 1940 and C. spatiosum Ozaki and Yagiu in Yagiu, 1944. The species of Condylostoma based on morphology are highly variable in most of characters and distinguished as following. Condylostoma curva is oval to elongate ellipsoidal, size about $245{\times}100{\mu}m$ in vivo, and conspicuously dark greenish brown with cortical granules on their surface, buccal cavity relatively large about 40% of body length, 5-11 macronuclear nodules, 4-8 frontal cirri serially arranged and 22-38 somatic kineties. Condylostoma minutum is elongate ellipsoidal and the size about $310{\times}55{\mu}m$ in vivo, buccal field about 35% of body length, 12-20 macronuclear nodules, adoral zone consisted of 82-107 membranelles, large and long one frontal cirrus and 38-44 somatic kineties. Condylostoma spatiosum is large elongate ellipsoidal, size about $670{\times}105{\mu}m$ in vivo, buccal cavity about 25% of body length, 11-25 macronuclear nodules, 111-144 adoral membranelles, membrane-like 2 frontal cirri, 49-74 somatic kineties. These three Condylostoma species are described here for the first time in Korea.

Arabidopsis thaliana Remorins Interact with SnRK1 and Play a Role in Susceptibility to Beet Curly Top Virus and Beet Severe Curly Top Virus

  • Son, Seungmin;Oh, Chang Jae;An, Chung Sun
    • The Plant Pathology Journal
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    • v.30 no.3
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    • pp.269-278
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    • 2014
  • Remorins, a family of plant-specific proteins containing a variable N-terminal region and conserved C-terminal domain, play a role in various biotic and abiotic stresses, including host-microbe interactions. However, their functions remain to be completely elucidated, especially for the Arabidopsis thaliana remorin group 4 (AtREM4). To elucidate the role of remorins in Arabidopsis, we first showed that AtREM4s have typical molecular characteristics of the remorins, such as induction by various types of biotic and abiotic stresses, localization in plasma membrane and homo- and hetero-oligomeric interaction. Next, we showed that their loss-of-function mutants displayed reduced susceptibility to geminiviruses, Beet Curly Top Virus and Beet Severe Curly Top Virus, while overexpressors enhanced susceptibility. Moreover, we found that they interacted with SnRK1, which phosphorylated AtREM4.1, and were degraded by the 26S proteasome pathway. These results suggest that AtREM4s may be involved in the SnRK1-mediated signaling pathway and play a role as positive regulators of the cell cycle during geminivirus infection.

Estimation for Seaweed Biomass Using Regression: A Methodological Approach (회귀분석을 이용한 해조류 생물량 측정을 위한 방법론)

  • Ko, Young-Wook;Sung, Gun-Hee;Kim, Jeong-Ha
    • ALGAE
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    • v.23 no.4
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    • pp.289-294
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    • 2008
  • To estimate seaweed biomass or standing crop, a nondestructive sampling can be beneficial because of not much destroying living plants and saving time in field works. We suggest a methodological procedure to estimate seaweed biomass per unit area in marine benthic habitats by using species-specific regression equations. Percent cover data are required from the field samplings for most species to convert them to weight data. However, for tall macroalgae such as kelps we need density data and their size (e.g., size class for subtidal kelps) of individuals. We propose that the field sampling should be done with 5 replicates of 50 cm x 50 cm quadrat at three zones of intertidals (upper, middle, lower) and three depth points (1, 5, 10 m) in subtidals. To obtain a reliable regression equation for a species, a substantial number of replicate is necessary from destructive samplings. The regression equation of a species can be further specified by different locality and different season, especially for the species with variable morphology temporally and spatially. Example estimation carried out in Onpyung, Jeju Island, Korea is provided to compare estimated values with real weight data.

A study of the electrical neuronal model (신경세포의 전기적 모델화)

  • 박상희;이명호
    • 전기의세계
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    • v.24 no.6
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    • pp.97-101
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    • 1975
  • The electrical neuronal model described in this paper simulates the most important functional properties of nerve cells. An model circuit incorporating many of the digital and analog properties of neurons is described. Having such properties as variable threshold level, action potential, summation, all-or-none output, absolute and relative refract oriness, and ingibition, it exhibits a considerable amount of functional equivalence to biological structures. This electrical neuronal model has utility not only for studying single unit properties but also for investigating group interactions. Such studies may be relevent to elucidation of neuronal network behavior.

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Cluster Analysis of the Foliose Lichens in Mt. Duckyoo (덕유산 엽상지의식물의 집락분석)

  • Park, Seung Tai
    • The Korean Journal of Ecology
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    • v.6 no.2
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    • pp.145-151
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    • 1983
  • The epiphytic lichen communities were analysed in terms of cluster analysis on forty two stands and eight environmental variables in Mt. Duckyoo. Ordination of stand and species by principal component analysis (PCA) and sum of square algorithm (SSA) gave similar results. Species cluster showed three groups(I, II, III) and stand revealed three groups (A, B, C). Interaction of stand and species cluster was interpreted by analysis of concentration technique. The results indicated a significant cluster structure at the level of different environment variable.

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A study on compensation of arousal level criteria (각성도 평가기준 보상법에 대한 연구)

  • Ko, H.W.;Kim, Y.H.;Lee, G.K.
    • Proceedings of the KOSOMBE Conference
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    • v.1996 no.11
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    • pp.86-89
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    • 1996
  • This paper describes compensation method to evaluate arousal level in different initial arousal state. Arousal level was measured by the relationship between IRI and Nz. The value of Nz is affected by BI which is variable with initial drowsiness. we introduced a correction factor to compensate these differences.

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