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http://dx.doi.org/10.5423/PPJ.OA.06.2014.0061

Arabidopsis thaliana Remorins Interact with SnRK1 and Play a Role in Susceptibility to Beet Curly Top Virus and Beet Severe Curly Top Virus  

Son, Seungmin (School of Biological Sciences, College of Natural Sciences, Seoul National University)
Oh, Chang Jae (School of Biological Sciences, College of Natural Sciences, Seoul National University)
An, Chung Sun (School of Biological Sciences, College of Natural Sciences, Seoul National University)
Publication Information
The Plant Pathology Journal / v.30, no.3, 2014 , pp. 269-278 More about this Journal
Abstract
Remorins, a family of plant-specific proteins containing a variable N-terminal region and conserved C-terminal domain, play a role in various biotic and abiotic stresses, including host-microbe interactions. However, their functions remain to be completely elucidated, especially for the Arabidopsis thaliana remorin group 4 (AtREM4). To elucidate the role of remorins in Arabidopsis, we first showed that AtREM4s have typical molecular characteristics of the remorins, such as induction by various types of biotic and abiotic stresses, localization in plasma membrane and homo- and hetero-oligomeric interaction. Next, we showed that their loss-of-function mutants displayed reduced susceptibility to geminiviruses, Beet Curly Top Virus and Beet Severe Curly Top Virus, while overexpressors enhanced susceptibility. Moreover, we found that they interacted with SnRK1, which phosphorylated AtREM4.1, and were degraded by the 26S proteasome pathway. These results suggest that AtREM4s may be involved in the SnRK1-mediated signaling pathway and play a role as positive regulators of the cell cycle during geminivirus infection.
Keywords
Arabidopsis; geminivirus; remorin; SnRK1;
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