• Title/Summary/Keyword: Biological sample

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Evaluation of Cyantraniliprole Residues Translocated by Lettuce, Spinach and Radish (상추, 시금치 및 알타리무에 의한 Cyantraniliprole의 흡수이행 잔류량 평가)

  • Yoon, Ji Hyun;Lee, Seung Won;Lim, Da Jung;Kim, Seon Wook;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.40 no.4
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    • pp.335-344
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    • 2021
  • BACKGROUND: Cyantraniliprole is a systemic diamide insecticide that has been used to control lepidopteran pests in agriculture. Cyantraniliprole has become an issue due to its potentiality of unexpectable contamination in rotational crop cultivation. Thus, studies on the evaluation of cyantraniliprole translocated from soil into rotational crops are required. METHODS AND RESULTS: Cyantraniliprole was treated at a yearly maximum application level onto bare soil under greenhouse conditions in two geographically different regions. Lettuce was transplanted and spinach and radish were sown onto the soil 30 and 60 days-plant back intervals (PBIs) after cyantraniliprole treatment. The QuEChERS method was modified and coupled with LC/MS/MS analysis to determine the residues of cyantraniliprole in soil and plant samples. The methods for sample preparation and instrumental conditions were validated to meet the criteria of Codex guidelines and were successful to determine cyantraniliprole quantitatively and qualitatively in the samples. Cyantraniliprole residues in lettuce samples were 0.01 mg/kg for PBI 60 and 0.02 mg/kg for PBI 30, respectively. The residues in spinach samples were 0.01 mg/kg for PBI 60 and 0.01~0.02 mg/kg for PBI 30, respectively. Less than limit of the quantitation (LOQ) level (0.01 mg/kg) of cyantraniliprole was observed in radish samples. The residues in the plant samples were found as the levels less than maximum residue limit (MRL) for leafy and root vegetables. CONCLUSION(S): This study suggests PBI 30~60 days for rotational cultivation of lettuce, spinach and radish in greenhouse soil treated with cyantraniliprole at a yearly maximum application level.

Effects of Advanced Oxidation of Penicillin on Biotoxicity, Biodegradability and Subsequent Biological Treatment (고도산화공정 처리가 페니실린의 생독성, 생분해도 및 생물학적 분해에 미치는 영향)

  • Luu, Huyen Trang;Minh, Dang Nhat;Lee, Kisay
    • Applied Chemistry for Engineering
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    • v.29 no.6
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    • pp.690-695
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    • 2018
  • Advanced oxidation processes (AOPs) composed of O3 and UV were applied to degrade penicillin (PEN). The degradation efficiency was evaluated in terms of changes in the absorbance (ABS) and total organic carbon (TOC). The combination of $O_3/H_2O_2/UV$ and $O_3/UV$ showed the best performance for the reduction of ABS (100% for 9 min) and TOC (70% for 60 min) values, although the mineralization was uncompleted under the experimental condition in this study. The change in biotoxicy was monitored with Escherichia coli susceptibility and Vibrio fischeri biofluorescence. The E. coli susceptibility was eliminated completely for 9 min by $O_3/UV$, and the toxicity to V. fischeri biofluorescence was 57% reduced by $O_3/H_2O_2/UV$. For the ultimate treatment of PEN, it is suggested that an AOP using $O_3/UV$ is followed by biological treatment, utilizing the enhanced biodegradability by the AOP. During 30 min of $O_3/UV$ treatment, the $BOD_5/COD$ ratio as an indication of biodegradability showed about 4-fold increment, compared to that of using a non-treated sample. TOC removal rate for AOP-pretreated PEN wastewater increased 55% compared to that of using the non-pretreated one through an aerobic biological treatment by Pseudomonas putida for artificial wastewater containing 20 mg/L of PEN. In conclusion, $O_3/UV$ process is recommended as a pretreatment step prior to an aerobic biological process to improve the ultimate degradation of penicillin.

Relationship between Environmental Exposure and Biological Monitoring Values in Workers Exposed to Styrene (스타이렌 폭로 근로자의 환경중 폭로농도와 생물학적 모니터링에 관한 연구)

  • Paik, Jong-Min;Lee, Jong-Yung;Kim, Jung-Man
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.7 no.2
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    • pp.161-170
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    • 1997
  • This is an effort to confirm changes biological monitoring according to changes in levels of exposure to styrene for industrial workers. This study was conducted on 108 workers, including male of 64 and female 44 who were working at factories of FRP, dipping, and coating. An improved passive monitor method(organic vapor monitor; OVM) was employed to determine levels of exposure. The biological monitoring include blood styrene concentration, urinary mandelic acid(MA), and urinary phenylglyoxylic acid(PGA). Biological monitoring were made through the Collection of blood and urine. The mean value of exposure to styrene was 21.0ppm, which is measured by organic vapor monitor, one of improved passive monitors. The highest exposure level was observed among workers in boat factories, laminating procedure workers, processing workers, respectively(p<0.01). For exposure level, 11% of subjects under study showed over 50ppm which is time weighted average(TWA). The correlation coefficient between biological specimens and the exposure level was 0.62 for blood styrene concentration, 0.58 for MA corrected by creatinine, and 0.70 for PGA corrected by creatinine, respectively(p<0.01). The regression analyses found exposure level relative importance in explaining variance in biological monitoring. In additional to that, gender was a significant factor in explaining variance of MA and MA+PGA. Almost half of variance(49%) in blood styrene concentration was explained by predictors, including exposure level, age, gender, duration, and drinking volume during the last week(p<0.01). The very high correlation(higher than 0.95 was found when a comparison was made among three types of corrected methods, including uncorrected specific gravity and creatinine. In conclusion, these findings suggest OVM to represent levels of exposure to styrene for industrial workers. A discussion was made on possible use of specific gravity sample for biological monitoring. Exposure level may be predicted on MA, PGA in urine, which could be applied to represent biological monitoring.

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Microfluidic Device for Bio Analytical Systems

  • Junhong Min;Kim, Joon-Ho;Kim, Sanghyo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.2
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    • pp.100-106
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    • 2004
  • Micro-fluidics is one of the major technologies used in developing micro-total analytical systems (${\mu}$-TAS), also known as “lab-on-a-chip”. With this technology, the analytical capabilities of room-size laboratories can be put on one small chip. In this paper, we will briefly introduce materials that can be used in micro-fluidic systems and a few modules (mixer, chamber, and sample prep. modules) for lab-on-a-chip to analyze biological samples. This is because a variety of fields have to be combined with micro-fluidic technologies in order to realize lab-on-a-chip.

Young Adults′Perceptions of Meanings and Motivations of Parenthood (부모됨의 의미와 동기에 대한 청년의 인식)

  • 유계숙;정현숙
    • Journal of Families and Better Life
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    • v.20 no.3
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    • pp.39-47
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    • 2002
  • This study analyses young adults'perceptions of the meanings and motivations of parenthood. It also verifies the relationship between the meanings and motivations of parenthood by young adults. The sample population included 311 university students. Our findings show that young adults perceived parenthood variously as a personal lifestyle, and as an statement of social maturation and sound mind and of marital satisfaction. There were no differences by gender in their perceptions on the meaning of parenthood. As for the motivation to become parents, strengthening biological family ties, traditional norms, emotional and altruistic reasons, deepening marital bonds, and perpetuating one's self through the passing on of genes. Among these, altruistic-emotional motivation and marital cohesion were stronger than other motivations. Finally, we provide recommendations for future research.

Characteristics of Korean Soybean Paste (Doenjang) Prepared by the Fermentation of Black Soybeans

  • Kim, Seong-Yeong;Son, Heung-Soo;Oh, Sung-Hoon
    • Preventive Nutrition and Food Science
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    • v.14 no.2
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    • pp.134-141
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    • 2009
  • The changes in components and biological activities of doenjang samples prepared with black soybeans and fermented with Bacillus subtilis SCB were investigated. The amino nitrogen (A-N) contents of samples increased with increasing black soybean content. A doenjang product made using a 1:1 ratio of soybeans-black soybeans showed a maximum level of genistein and daidzein isoflavones ($1111.6{\mu}g/g$) at 110 days of fermentation, along with decreasing contents of genistin and daidzin due to the conversion to aglycones. The black soybean-only doenjang sample showed higher protease activity, including caseinolytic and fibrinolytic enzyme activities, than the other samples, and had relatively higher polyphenol content and DPPH radical scavenging activity. Therefore, doenjang made with additions of black soybeans and fermented by B. subtilis SCB may have improved physiological properties, suggesting this to be a valuable method of preparation.

Study on Surface Damage of Specimen for Transmission Electron Microscopy(TEM) Using Focused Ion Beam(FIB) (집속 이온빔을 이용한 투과 전자 현미경 시편의 표면 영향에 관한 연구)

  • Kim, Dong-Sik
    • 전자공학회논문지 IE
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    • v.47 no.2
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    • pp.8-12
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    • 2010
  • TEM is a powerful tool for semiconductor material analyses in structure or biological sample in micro structure. TEM observation need to make to coincide specimens for special purpose. in this paper, we have experimented for minimum surface damage on bulk wafer and patterned specimen by various conditions such as accelerating energy, depth of ion beam, ion milling types, and etc. in various specimen preparation methods by FIB (Focus Ion Beam). The optimal qualified specimens are contain low mounts of surface damage(about 5 nm) on patterned specimen.

Flame Atomic Absorption Spectrometric Determination of Trace Amounts of Thallium after Solid-Liquid Extraction and Preconcentration with Use of 1,10-Phenanthroline onto Benzophenone

  • Taher, Mohammad Ali
    • Bulletin of the Korean Chemical Society
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    • v.24 no.8
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    • pp.1177-1180
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    • 2003
  • Thallium is quantitatively retained by 1,10-phenanthroline and tetraphenylborate onto benzophenone in the pH range 0-11 from a large volumes of aqueous solutions of various samples. After filtration, the solid mass consisting of thallium complex and benzophenone is dissolved with 5 mL of dimethylformamide and the metal was determined by flame atomic absorption spectrometric. About 0.4 ㎍ of thallium can be concentrated from 400 mL of aqueous sample, where its concentration is as low as 1.0 ng/mL. Eight replicate determinations of 8.0 ㎍/mL of thallium in final dimethylformamide solution gave a mean absorbance of 0.160 with a relative standard deviation of 1.7%. The sensitivity for 1% absorption was 0.22 ㎍/mL. The interference of a large number of anions and cations has been studied and the optimized conditions developed were utilized for the trace determination of thallium in various alloys and biological samples.

Real Time Implementittion of Time Varying Nonstationary Signal Identifier and Its Application to Muscle Fatigue Monitoring (비정상 시변 신호 인식기의 실시간 구현 및 근피로도 측정에의 응용)

  • Lee, Jin;Lee, Young-Seock;Kim, Sung-Hwan
    • Journal of Biomedical Engineering Research
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    • v.16 no.3
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    • pp.317-324
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    • 1995
  • A need exists for the accurate identification of time series models having time varying parameters, as is important in the case of real time identification of nonstationary EMG signal. Thls paper describes real time identification and muscle fatigue monitoring method of nonstationary EMG signal. The method is composed of the efficient identifier which estimates the autoregressive parameters of nonstationary EMG signal model, and its real time implementation by using T805 parallel processing computer. The method is verified through experiment with real EMG signals which are obtained from surface electrode. As a result, the proposed method provides a new approach for real time Implementation of muscle fatigue monitoring and the execution time is 0.894ms/sample for 1024Hz EMG signal.

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Implementation of Real Time 3 channel Transmission System Using ECG Data Compression Algorithm by Max-Min Slope Update (최대 및 최소 기울기 갱신에 의한 ECG 압축 알고리듬을 이용한 실시간 3채널 전송시스템 구현)

  • 조진호;김명남
    • Journal of Biomedical Engineering Research
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    • v.16 no.3
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    • pp.271-278
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    • 1995
  • An ECG data compression algorithM using max-min slope update is proposed and a real time 3 channel ECG transmission system is implemented using the proposed algorithm. In order to effectively compress ECG data, we compare a threshold value with the max-min slope difference (MMSD) which is updated at each sample values. If this MMSD value is smaller than the threshold value, then the data is compressed. Conversely, when the MMSD value is larger than threshold value, the data is transmitted after storing the value and the length between the data which is beyond previous threshold level. As a result, it can accurately compress both the region of QRS, P, and T wave that has fast-changing and the region of the base line that slope is changing slow. Therefore, it Is possible to enhance the compression rate and the percent roms difference. In addition, because of the simplicity, this algorithm is more suitable for real-time implementation.

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