• Applied Biological Chemistry
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• v.41 no.8
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• pp.583-586
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• 1998

Porous ceramic cups are used for monitoring ion concentration in soil solutions in various time course and depth. A soil solution sampler was constructed in laboratory by inserting pliable perfluoroalkoxy(PFA) tubings into porous cup through holes in PVC rod segment which plugged top opening of the porous cup. The system was installed in drip irrigated soil in a vertical position, and nitrogen movement below the drip basin was monitored. To collect soil solution, vacuum in the cup was applied with a hand vacuum pump. The samples obtained were sufficient enough to run quantitative analyses for a number of chemicals. Nitrogen transformation and movement could be well defined, and the system seemed to be relevant to the other soil solution samplers in monitoring chemical movement in soil. Although this system has general deficiencies found in the other samplers using ceramic cup, it could be easily constructed at a low cost. Since the tubing was pliable, the cups could be installed in horizontal position, and this allows installations of the cups at more precise depth increments and also more precise samplings of soil solution at each depth.

• KSBB Journal
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• v.13 no.4
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• pp.461-467
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• 1998

A fermentation system was supplemented with a device for the measurement of the durations of alkali pump feeding for automatic pH control and an A/D convertor for precise monitoring of pH value by computer. A software program was developed to measure buffer capacities from the pH signal and the pH control signal during fermentation. By measuring the buffer capacity on-line, levels of acetic acid were estimated by a software sensor using pH signal in a fermentation process of E.coli growing in a minimal medium. The measured values of acetic acid showed correlation to those of estimated by the software sensor. Lacitic acid production was also successfully estimated by the values of buffer capacities measured on-line.

• Journal of Soil and Groundwater Environment
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• v.17 no.6
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• pp.23-32
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• 2012

This study was conducted to monitor microbial species dynamics within the aquifer due to long term operation of geothermal heat pump system. The species were identified by molecular biological methods of 16S rDNA. Groundwater sample was collected from both open (S region) and closed geothermal recovery system (J region) along with the control. J measured and control as well as S measured found Ralstonia pickettii as dominant species at year 2010. In contrast, Rhodoferax ferrireducens was dominantly observed for the control of S. In 2011, Sediminibacterium sp. was universely identified as the dominant species regardless of the monitoring places and type of sample, i.e., measured or control. The difference in the dynamics between the measured and the control was not critically observed, but annual variation was more strikingly found. It reveals that possible environmental changes (e.g. ORP and DO) due to the operation of geothermal heat recovery system in aquifer could be more exceedingly preceded to differentiate annual variation of microbial species rather than positional differences.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1605-1612
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• 2016

Quinolone-resistant Salmonella strains were isolated from patient samples, and several quinolone-sensitive strains were used to analyze mutations in the quinolone resistance-determining region (QRDR) of gyrA, gyrB, parC, and parE and to screen for plasmid-mediated quinolone resistance. Among the 21 strains that showed resistance to nalidixic acid and ciprofloxacin (MIC 0.125-2.0 μg/ml), 17 strains had a mutation in QRDR codon 87 of gyrA, and 3 strains had a single mutation (Ser83 → Phe). Another cause of resistance, efflux pump regulation, was studied by examining the expression of acrB, ramA, marA, and soxS. Five strains, including Sal-KH1 and Sal-KH2, showed no increase in relative expression in an analysis using the qRT-PCR method (p < 0.05). In order to determine the genes involved in the resistance, the Sal-9 isolate that showed decreased susceptibility and did not contain a mutation in the gyrA QRDR was used to make the STM (MIC 8 μg/ml) and STH (MIC 16 μg/ml) ciprofloxacin-resistant mutants. The gyrA QRDR Asp87 → Gly mutation was identified in both the STM and STH mutants by mutation analysis. qRT-PCR analysis of the efflux transporter acrB of the AcrAB-TolC efflux system showed increased expression levels in both the STM (1.79-fold) and STH (2.0-fold) mutants. In addition, the expression of the transcriptional regulator marA was increased in both the STM (6.35-fold) and STH (21.73-fold) mutants. Moreover, the expression of soxS was increased in the STM (3.41-fold) and STH (10.05-fold) mutants (p < 0.05). Therefore, these results indicate that AcrAB-TolC efflux pump activity and the target site mutation in gyrA are involved in quinolone resistance.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.26 no.2
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• pp.145-166
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• 2021

For several decades, sediment traps have served as one of the key tools for constraining the biological carbon pump (BCP), a process that vertically exports particulate organic carbon (POC) and associated biogenic materials from marine primary production in surface waters to the deep ocean interior. In this paper, I introduced the general methods, the current status of global sediment trap studies, and importance of it to understand the deep ocean carbon cycling. Recent studies suggest that sinking POC in the deep ocean are more complex and spatio-temporally heterogeneous than we considered. Especially researches those studied resuspended and laterally transported particles are presented. Researches that used organic (radiocarbon; ¹⁴C) and inorganic (Al) tracers to understand the oceanic POC cycling and the significance of resuspended particles are reviewed, and the importance of radiocarbon study by using MICADAS (Mini radioCarbon Dating Systems) is emphasized.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.18-21
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• 1995

When we inject drugstuffs to a patient for a long time, it is important to control proper injection amount and flow rate. Since inproper injection amount and a flow rate would cause bad a recovery a patient, the relience of sensors which detect injection amount is an important factor for whole injection systems' performance. In this research, we've compared the suitability of three sensors for injection pump monitoring system. The three types of sensors, piezo film sensor, photo transistor made up with three transmitting photodiodes and receving photodiode, and photo array, were selected for comparing. Using suggested data processing technique and photo amy sensors, we could minimize the effect of interference, disturbance, illumanation change, and sensitivity change caused by sensor's position. According to the experiments, the photo amy showed the higher reliance than any other the three types of sensors. The developed systems could be the foundation of beginning home production of infusion pump system and available for the base model of whole monitoring and control systems.

• 한국전산유체공학회:학술대회논문집
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• 2008.03b
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• pp.105-106
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• 2008

As a carrier of malaria and sneak of blood, mosquitoes are an unpleasant insect. However, there are several unknown natural secretes related with mosquitoes. Among them, we focused on the blood sucking process of a female mosquito. The main objective of this study is to understand the mosquito's blood sucking mechanism that can be used to resolve the problem encountered in the injection or transport of infinitesimal biological fluids in a micro-chip. At first, the velocity fields of blood-sucking flow in a proboscis were measured using a micro-particle image velocimetry (PIV) technique. The velocity signals of flow in the proboscis show periodic variation. This seems to be resulted from the beating of the pharyngeal pump which works as driving power. To analyze the pumping mechanism, the temporal variation of the pharyngeal pump was visualized using the synchrotron X-ray micro-imaging technique. The volume variation was estimated by the help of digital image processing techniques. Once the main mechanism of blood sucking process was found, a effective micro-pumping system with high efficiency would be developed in near future.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.05
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• pp.247-250
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• 1995

The purpose of this paper is to develop diaphragm-type pneumatic ventricular assist device(VAD) for clinical application and to evaluate its performance through the mock circulation system. The blood housing and diaphragm are made by coating pellethane on the metallic mold and the back plate is made by machining process. The relations of cardiac output(CO) vs. beat rate and CO vs. systolic-to-diastolic rate was estimated through the mock test and hemodynamic waves are recorded for the evaluation of VAD. As a result, the volume of blood pump is 70 ml, maximum CO is 5 L/min and CO has a close relation to the input resistance of blood pump. The hemodynamic data and waves showed this system can be applicable to the animal experiment.

• Proceedings of the KOSOMBE Conference
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• v.1996 no.05
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• pp.207-212
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• 1996

We developed pneumatic blood pump and its driving system as a pediatric ventricular assist device. The blood pump is diaphragm type system and its blood contacting area is coated with Bio-Span. The driving unit Is consists of dual pumps, valves for the reliable blood pumping and its controller uses 80C196(Intel) as a main processor. The acute animal experiment was performed with dogs and its body weight is about 20 kg. The maximum cardiac output is about 2.1 l/min and the pressure and flow curves showed reliable operation as assist device.

• Proceedings of the KOSOMBE Conference
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• v.1995 no.11
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• pp.216-218
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• 1995

A new atrial pressures balancing method for moving actuator total artificial heart(TAH) without an extra compliance chamber is developed. The structural characteristics of the pendulous moving actuator have made it possible to compensate the left and right pump output difference by utilizing the interventricular air space as an internal compliance chamber in a pump housing. Furthermore, the balancing performance is increased through the improvement of the flexibility of part of the polyurathane housing. However, the increase of the flexibility of the housing causes a little loss of the cardiac output due to the reduction of active filling performance. In this paper, a good condition between the balance and pump output performance is evaluated by adjusting the air volume in the interventricular space through a series of in vitro experiment.