Myeong-Eun Jegal;Yu-Seon Han;Shi-Yung Park;Ji-hyeok Lee;Eui-Yun Yi;Yung-Jin Kim
Journal of Life Science
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v.33
no.9
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pp.703-712
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2023
Angiogenesis, the formation of blood vessels from pre-existing vessels, is a multistep process regulated by modulators of angiogenesis. It is essential for various physiological processes, such as embryonic development, chronic inflammation, and wound repair. Dysregulation of angiogenesis causes many diseases, such as cancer, autoimmune diseases, rheumatoid arthritis, cardiovascular disease, and delayed wound healing. However, the number of effective anti-angiogenic drugs is limited. Recent research has focused on identifying potential drug candidates from natural sources. For example, marine natural products have been shown to have anti-cancer, anti-oxidant, anti-inflammatory, antiviral, and wound-healing effects. Thus, this study aimed to describe the angiogenesis inhibitory effect of Hizikia fusiforms (brown algae) extract. The hexane extract of H. fusiformis has shown inhibitory effects on in vitro angiogenesis assays, such as cell migration, invasion, and tube formation in human umbilical vein endothelial cells (HUVECs). The hexane extract of H. fusiformis (HFH) inhibited in vivo angiogenesis in a mouse Matrigel gel plug assay. In addition, the protein expression of vascular endothelial growth factor (VEGF), mitogen-activated protein kinase (MAPK)/extracellular signal kinase, and AKT serine/threonine kinase 1 decreased following treatment with H. fusiformis extracts. Our results demonstrated that the hexane extract of H. fusiformis (HFH) inhibits angiogenesis in vitro and in vivo.
Myeong-Eun Jegal;Yu-Seon Han;Shi-Young Park;Ji-Hyeok Lee;Eui-Yeun Yi;Yung-Jin Kim
Journal of Life Science
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v.34
no.6
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pp.399-407
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2024
Angiogenesis is the process by which new blood vessels form from existing blood vessels. This phenomenon occurs during growth, healing, and menstrual cycle changes. Angiogenesis is a complex and multifaceted process that is important for the continued growth of primary tumors, metastasis promotion, the support of metastatic tumors, and cancer progression. Impaired angiogenesis can lead to cancer, autoimmune diseases, rheumatoid arthritis, cardiovascular disease, and delayed wound healing. Currently, there are only a handful of effective antiangiogenic drugs. Recent studies have shown that natural marine products exhibit antiangiogenic effects. In a previous study, we reported that the hexane extract of H. fusiformis (HFH) could inhibit the development of new blood vessels both in vitro and in vivo. The aim of this study was to describe the inhibitory effect of chloroform extracts of H. fusiformis on angiogenesis. To investigate how chloroform extract prevents blood vessel growth, we examined its effects on HUVEC, including cell migration, invasion, and tube formation. In a mouse Matrigel plug assay, H. fusiformis chloroform extract (HFC) also inhibited angiogenesis in vivo. Certain proteins associated with blood vessel growth were reduced after HFC treatment. These proteins include vascular endothelial growth factor (VEGF), mitogen-activated protein kinase (MAPK)/extracellular signal transduction kinase, and serine/threonine kinase 1 (AKT). These studies have shown that the chloroform extract of H. fusiformis can inhibit blood vessel growth both in vitro and in vivo.
Park, Sang Ryeol;Kim, Hye Seon;Lee, Kyong Sil;Hwang, Duk-Ju;Bae, Shin-Chul;Ahn, Il-Pyung;Lee, Seo Hyun;Kim, Sun Tae
Journal of Plant Biotechnology
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v.44
no.2
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pp.149-155
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2017
Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58-overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.
Changes of geomorphology in alluvial river and vegetation recruitment on its floodplain downstream from dams are investigated both qualitatively and quantitatively focusing on the downstream of Andong dam and Imha dam on the Nakdong River. Results of the analyses of river morphology and bed material in the study site show a general trend of riverbed degradation with a max scour of 3 m and bed material coarsening from pre-dam value of 1.5 mm in D50 to post-dam value of 2.5 mm. Decrease in bed shear stress due to the decrease in flood discharge have caused vegetation recruitment on the once-naked sandbars. As result, the ratio of area of vegetated bars over total area of bars has drastically changed from only 7% in 1971 before the Andong dam (constructed in 1976) to 25% after it, and increased to 43% only three year after the Imha dam (constructed in 1992) and eventually to 74% by 2005. Analysis of the vegetation succession at Wicjeol subreach, one of the three subreaches selected in this study for detailed investigation, has clearly shown a succession of vegetation on once-naked sand bars to a pioneering stage, reed and grass stage, willow shrub and eventually to willow tree stages. At the second subreach selected, two large point bars in front of Hahoe Village seem to have maintained their sand surfaces without a signifiant vegetation recruitment until 2005. The sand bars, however, seem to have been invaded by vegetation recently, which warns river managers to have a countermeasure to protect the sand bars from vegetation invasion in order to conserve them for the historical village of Hahoe. On the other hand, recruitment and establishment of vegetation on the sand bars by artificial disturbance of the river, such as damming, can create an unique habitat of backmarsh in the sandy river, as shown in the case of Gudam Wetland, and may increase the biodiversity as compared with relatively monotonous sand bars. Last, the premise in this study that decrease in flood discharge due to upstream dams and decrease in bed shear stress can induce vegetation recruitment on the naked sand bars in the river has been verified with the analyses of the distribution of dimensionless bed shear stress along the selected cross section in each subreach.
The objective of this study was to identify the life form characteristics, naturalized degree and introduction period of naturalized plants on upland fields in South Korea. The survey sites were selected in 222 upland fields over 9 provinces where are relatively dry soil conditions and are disturbed continuously by farming practices. It seemed that these uplands maintain good condition for the invasion and settlement of alien plants. Field surveys were carried out twice from May to June and from August to September in 2013. The vascular plants of the upland fields were listed into 539 taxa which contain 103 families, 320 genera, 448 species, 2 subspecies, 74 varieties and 15 forms. Ninety nine taxa were naturalized plants composed of 23 families, 64 genera, 91 species and 8 varieties. The urbanization index and naturalized index is 30.65% and 18.37%, respectively. Among total 99 naturalized plants, Compositae (30 taxa) was the most dominant family, and followed by Gramineae (14 taxa). On the basis of dormancy form, 78 taxa were belong to Th (Therophyte). In radicoid form, $R_5$ plants (86 taxa) and $R_3$ plants (14 taxa) were dominantly distributed. In disseminule form, $D_4$ including 63 taxa and $D_2$ containing 26 taxa were dominant. On the basis of growth form, erect form (e) containing 26 taxa was dominant. In conclusion, life form spectrum was $Th-R_5-D_4-e$ type. According to life form analysis, naturalized degree and introduction period, alien plants with unique characteristics may have high ability to settle and spread nationwide after inflow into South Korea. It needs further to observe some plant species with or without ecological features such as widely disseminated seeds by wind or water, high seed productivity, long life-span seeds, high germination rate of seeds, or non-dormant germinable seed.
Journal of the Korean Institute of Landscape Architecture
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v.42
no.4
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pp.48-59
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2014
This study was carried out to present raw data on managing the restored urban stream by studying the naturalized plants distributed in Gaeumjeong Stream, Changwon-si, Gyeongsangnam-do, Korea. The results were as follows. The numbers of naturalized plants were summarized as 45 taxa including 17 families, 36 genera, 43 species and 2 varieties. The invasive alien plants were 2 taxa including Ambrosia artemisiifolia and Lactuca sativa. The following summarizes the attributes of the naturalized plants. Most of the plants commonly originated from Europe and North America. The 5 naturalized degree that was widely distributed and had many individual was the most common. Until 1921, after the opening of 1 period was the most common in the introduced period. Section 12 had the highest NI at 41.9%, and the lowest, at 20.5%, in sections 9 and 19 were analyzed. Section 1 had the highest UI at 6.2%, whereas, the lowest, at 2.5%, was calculated in sections 19 and 20. Section 2 showed the highest DI at 16.7%. The first results of the analysis of the causes for the invasion of naturalized plants on the riverside and waterways, and physical factors and maintenance are directly affected. Second, sewage, muddy water and sediment deposits this naturalized plant caused by a chemical factor. Third, it is thought that invasive alien plants are irregular as it happens in biological factor. The proposed management plan naturalized plants, the first, disturbance caused by species management is a young object is removed immediately before flowering scape to eliminate or suppress the propagation of physical methods will be needed. Second, the fact that the national spread of native plant species and planting management does not provide space for the growth is very important. Third, agricultural land is disturbed by agricultural practices by interfering with the action of naturalized plants because the source of the river should be prohibited in agriculture. In the future, if we studied the naturalized plants distributed in restored streams located in Changwon-si, the characteristics of change in the ecosystem impact is expected to be beneficial.
In infectious disease, invasion of host tissue by bacteria or their products frequently induces a wide variety of inflammatory and immunopathologic reaction. Evidence indicates that cytokines are involved in the initiation and progression of chronic inflammatory diseases, such as periodontitis. Interleukin-6, which is a multifunctional cytokine, has important roles in acute and chronic inflammation and may also be implicated in bone resorption. Periodontal diseases are characterized by chronic inflammation of the periodontium with alveolar bone resoption. A principal driving force behind this response appears to lie in the immune system's response to bacteria. Many of the cell components which have been shown to function as virulence factors in gram-negative bacteria are associated with the bacterial surface. Of these, lipopolysaccharide has been characterized as one that mediates a number of biological activities which can lead to the destruction of host tissue. Non-steroidal antiinflammatory drug is used for reduce inflammation, and most of NSAIDs inhibit prostaglandine $E_2$ production, but it is shown that $PGE_2$ production is stimulated by IL-1 in recent study. So, the influence of other cytokines except $PGE_2$ on periodontium can not be avoided. Therefore, new antiinflammatory drug is needed. Rhizoma coptidis is used in oriental medicine for anti-inflammation and antiseptics. In this present study, we examined the IL-6 release in periodontal ligament cells treated with the lipopolysaccharide, and also the effect of rhizoma coptidis on cellular activity and IL-6 production of periodontal ligament cells. To evaluate the effect of rhizoma coptidis on cellular activity, the cells were seeded at a cell density of $1{\times}10^4$ cells/well in 24-well culture plates. After one day incubation, 1-6, 10-9 and 10-12 g/ml of rhizoma coptidis and 5, $10{\mu}g/ml$ of LPS were added to the each well and incubated for 1 and 2 days, respectively. Then, MTT assay were carried out. To evaluate the effect of rhizoma coptidis on IL-6 production, the cells were seeded at a cell density of $1.5{\times}10^4$ cells/well in 24-well culture plates. After one day incubation, 10-9 g/ml of rhizoma coptidis and 5, $10{\mu}g/ml$ of LPS were added to the each well and incubated for 3, 6, 12 and 24 hours. Then, amounts of IL-6 production is measured by IL-6 ELISA kit used. The results were as follows : 1. Rhizoma coptidisrbelow to ($10^{-6}g/ml$) significantly increaed cellular activity of periodontal ligament cells than control. 2. Rhizoma coptidist ($10^{-9}g/ml$) significantly increased cellular activity of LPS($5{\mu}g/ml$)-treated periodontal ligament cells than control. 3. LPS(5 and $10{\mu}g/ml$) significantly increased IL-6 production of periodontal ligament cells than control. 4. Rhizoma coptidis($10^{-9}g/ml$) decreased IL-6 production of LPS ($5{\mu}g/ml$)-treated periodontal.ligarnent cells than LPS only tested group. These findings suggest that stimulation of the IL-6 release of periodontal ligament cells by LPS may have a role in the progression of inflammation and alveolar bone resoption in periodontal disease, and that inhibition of the IL-6 release of cells and stimulation of cellular activity by rhizoma coptidis may help the periodontal regeneration.
Background : RASSF1A, which is one of tumor suppressor genes, is frequently inactivated by hypermethylation of the promoter region in a variety of human cancers, including lung cancer. This study was performed to investigate the association between RASSF1A methylation and the clinicopathological factors in patients with squamous cell carcinoma of the lung. Methods : Eighty-one samples from the patients with squamous cell carcinoma of lung were examined. The promoter methyation of RASSF1A was analyzed by methylation specific PCR and sequencing. Statistical analysis was made to examine the association between RASSF1A methylation and the clinicopathological parameters. Results : RASSF1A methylation was observed in 37.0 % (30 of 81) of the patients with squamous cell carcinoma of the lung. RASSF1A methylation was found to be associated with cellular differentiation(p=0.0097) and the overall survival(p=0.0635). However, there was no association between RASSF1A methylation and the other clinicopathological parameters, such as the pathological TNM stage, the recurrence rate, lymph node invasion and the amount of cigarettes smoked. Conclusion : RASSF1A methylation might be associated with a poor prognosis in patients with squamous carcinoma of the lung. A larger scale study is needed.
Background: Reactive oxygen species are involved in multi-stage process of carcinogenesis. The moot of cancer cell lines and cancer cells in tumor tissue produce reactive oxygen species and on the other hand, the activities of catalase, Mn- and CuZn-superoxide dismutase in tumor cells are usually low. These persistent oxidative stress in tumor tissue facilitates tumor invasion and metastasis. 12-kDa thioredoxin, which regulates the intracellular redox potential with glutathione and glutaredoxin is involved in cell activation, proliferation, differentiation and redox-mediated apoptosis. It is also purified as 14-kDa and 10-kDa eooinophilic cytotoxic enhancing factor(ECEF) from human histiocytic cell(U937) and 10-kDa ECEF has more than 20 times eosinophilic stimulation activity than 14-kDa ECEF. It has been reported that adult T-cell leukemia, squamous cell carcinoma of uterine cervix, and hepatocellular carcinoma show increased amounts of human thioredoxin and thioredoxin mRNA is increased in lung cancer. In this study, we investigated the expression of conventional antioxidant enzymes such as catalase, CuZn-SOD, and glutathione peroxidase and thioredoxin in lung cancer tissue compared to adjacent normal lung tissue and the induction of thioredoxin in macrophage cells after treatment of oxidative stress and endotoxin Methods: We measured the amount of conventional antioxidant enzymes such as catalase, CuZn-SOD, and glutathione peroxidase and thioredoxin in lung cancer tissue compared to adjacent normal lung tissue by immunoblot analysis and the induction of thioredoxin in mouse monocyte-macrophage cells(RAW 264.7) by treatment of 5 ${\mu}M$ menadione and 1 ${\mu}g/ml$ endotoxin Results: On immunoblot analysis, the expression of 12-kDa thioredoxin was increased in lung cancer tissue compared to paired normal lung tissue. but the expression of catalase and CuZn-SOD were decreased in lung cancer tissue compared to paired normal tissue and the expression of glutathione peroxidase in lung cancer was variable. The expression of truncated thioredoxin was also increased in lung cancer. When mouse monocyte-macrophage cells were treated with 5 ${\mu}M$ menadione and 1 ${\mu}g/ml$ endotoxin, the expression of thioredoxin was peaked at 12 hrs and sustained to 48 hrs. Conclusion: In contrast with other conventional antioxidants, the expression of 12-kDa and truncated thioredoxin in lung cancer were increased and it is closely associated with persistent oxidative stress in tumor microenvironment. Considering especially the biological functions of truncated thioredoxin, the increased amount of truncated thioredoxin has significant role in tumor growth through cell proliferation.
The stone statues in the site of Mireuksaji Temple(Iksan, South Korea) were created after the stone pagoda was built in 639. They, transitional statues between animal sculptures and human shaped statues made from the late Goryeo dynasty until the early Joseon dynasty, were set up at the four corners of the stone pagoda by way of guardians. In the case of three statues, their surfaces were denudated and their iconographies have been indiscernible. However, the one in the southwest clearly shows its iconography. It is inferior in properties to the other three statues in the northwest, the northeast and the southeast respectively, but on the other hand its iconography has been well maintained. The reason is related to exposure to harmful environments; specifically, the retaining wall, built around the stone pagoda in the 17th century, had the southwest statue inside and could naturally worked as a buffer against harmful environments. As a result, for about 400 years there has been difference in weathering conditions between the three stone statues and the southwest statue, which brought denudation, the consequent indiscernibleness of iconography and biological invasion to the three statues, notwithstanding superior properties(northwest statue:$176kgf/cm^2$, northeast statue:$109kgf/cm^2$, southeast statue:$273kgf/cm^2$). In contrast, the southwest statue significantly shows its iconography with black contaminants and granule decomposition, despite inferior properties($133kgf/cm^2$). Defenseless exposure to external environment is not recommended for the stone statues, because it is hard to preserve the extant iconography. Herein lies the application of the data on microclimate around Mireuksaji Temple. As regards the weathering zone in which the stone statues are located, Conservation increases in acidity and frequency as years go by, Hereat, in the approach to the Conservation of stone statues, the first consideration needs to be morphological historicity rather than geographical location.
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