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http://dx.doi.org/10.5010/JPB.2017.44.2.149

Overexpression of rice NAC transcription factor OsNAC58 on increased resistance to bacterial leaf blight  

Park, Sang Ryeol (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Kim, Hye Seon (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Lee, Kyong Sil (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Hwang, Duk-Ju (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Bae, Shin-Chul (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Ahn, Il-Pyung (Gene Engineering Div., National Academy of Agricultural Science, Rural Development Administration)
Lee, Seo Hyun (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University)
Kim, Sun Tae (Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.2, 2017 , pp. 149-155 More about this Journal
Abstract
Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58-overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.
Keywords
Bacterial blight resistance; NAC transcription factor; OsNAC58; Rice; Xanthomonas oryzae pv. oryzae;
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