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Association between RASSF1A Methylation and Clinicopathological Factors in Patients with Squamous Cell Carcinoma of Lung  

Choi, Naeyun (Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Lee, Hye-Sook (Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Song, In Seung (Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Lim, Yu Sung (Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Son, Dae-Soon (Cancer Research Center, Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine)
Lim, Dae-Sik (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Choi, Yong Soo (Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Kim, Jhingook (Department of Thoracic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Kim, Hojoong (Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Publication Information
Tuberculosis and Respiratory Diseases / v.57, no.3, 2004 , pp. 265-272 More about this Journal
Abstract
Background : RASSF1A, which is one of tumor suppressor genes, is frequently inactivated by hypermethylation of the promoter region in a variety of human cancers, including lung cancer. This study was performed to investigate the association between RASSF1A methylation and the clinicopathological factors in patients with squamous cell carcinoma of the lung. Methods : Eighty-one samples from the patients with squamous cell carcinoma of lung were examined. The promoter methyation of RASSF1A was analyzed by methylation specific PCR and sequencing. Statistical analysis was made to examine the association between RASSF1A methylation and the clinicopathological parameters. Results : RASSF1A methylation was observed in 37.0 % (30 of 81) of the patients with squamous cell carcinoma of the lung. RASSF1A methylation was found to be associated with cellular differentiation(p=0.0097) and the overall survival(p=0.0635). However, there was no association between RASSF1A methylation and the other clinicopathological parameters, such as the pathological TNM stage, the recurrence rate, lymph node invasion and the amount of cigarettes smoked. Conclusion : RASSF1A methylation might be associated with a poor prognosis in patients with squamous carcinoma of the lung. A larger scale study is needed.
Keywords
Methylation; Squamous cell carcinoma; Tumor suppressor gene;
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