• 한국약용작물학회지
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• 제24권2호
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• pp.143-151
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• 2016

Background: The public has increasing concerns about herbal crops owing to insufficient information on biological hazards such as foodborne pathogens. Therefore, the objective of this study is the development of a herbal crop quality control system through monitoring with biological hazard analysis. Today, it is estimated that millions of people become ill every year from food contamination. The public demands agricultural products of stable and consistent quality. Governments have the responsibility of establishing the standards, legislation and enforcement programs necessary to control food quality and safety. However, research on the biosafety of herbal crop products is still insufficient. Therefore, the implementation of monitoring systems with high standards is critical for public safety. Methods and Results: In this study, we collected 52 samples of herbal crop products, and conducted both quantitative and qualitative biological hazard analysis. With biological hazard analysis, aerobic bacteria, Staphylococcus aureus, Salmonella spp., Escherichia coli, Coliforms, and Listeria spp. could be detected. Conclusions: Herbal crops were found to be contaminated with aerobic bacteria at $3.69{\pm}0.32log\;CFU/g$. Staphylococcus aureus, Salmonella spp., Escherichia coli, Coliforms, and Listeria spp. were not detected in any of the samples. This research suggests that continuous monitoring of biological hazards is required to improve the quality of herbal crops.

• 환경생물
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• 제39권2호
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• pp.169-183
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• 2021

본 연구는 강원도 영월군에 위치하는 한반도습지에 분포하는 관속식물상을 파악하여 내륙습지 생태계의 식물 자원 관리보전을 위한 기초자료로 활용하고자 하였다. 조사는 2019년 4월부터 2020년 5월까지 총 9회에 걸쳐 수행하였다. 조사결과 분포가 확인된 관속식물은 93과 309속 456종 10아종 37변종 5품종 등 총 508분류군이었다. 이 중 멸종위기 야생식물은 II급에 해당하는 2분류군이 확인되었으며, 희귀식물은 8분류군이었다. 특산식물은 8분류군이었으며, 식물구계학적 특정식물은 V등급에 3분류군, IV등급에 10분류군, III등급에 15분류군, II등급에 17분류군 그리고 I등급에 26분류군으로 총 71분류군이었다. 귀화식물은 57분류군이 확인되었으며, 이에 의한 귀화율은 11.2%, 도시화지수는 17.8%로 산출되었고, 생태계교란 야생식물은 5분류군이 분포하는 것으로 조사되었다. 본 연구결과는 람사르습지에 등재되어 있어 보전가치가 매우 높은 한반도 습지의 효율적인 관리를 위한 유용한 기초자료로 활용될 것으로 판단된다.

• Safety and Health at Work
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• 제14권4호
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• pp.390-397
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• 2023

Introduction: Biological risks are a major global problem in the workplace. The recent COVID-19 pandemic has highlighted the need for a more comprehensive understanding of the biological risks at work. This study presents data on both communicable infectious biological agents and noncommunicable factors leading to death and disability for the year 2021. Methods: We followed the methodology established by the International Labour Organization (ILO) in their past global estimates on occupational accidents and work-related diseases. We used relevant ILO estimates for hazardous substances and related population attributable fractions derived from literature, which were then applied to World Health Organization mortality data. The communicable diseases included in the estimates were tuberculosis, pneumococcal diseases, malaria, diarrheal diseases, other infectious diseases, neglected tropical diseases, influenza associated respiratory diseases and COVID-19. Noncommunicable diseases and injuries considered were Chronic Obstructive Diseases (COPD) due to organic dusts, asthma, allergic reactions and risks related to animal contact. We estimated death attributable to biological risk at work and disability in terms of disability adjusted life years (DALYs). Results: We estimated that in 2022, 550,819 deaths were caused by biological risk factors, with 476,000 deaths attributed to communicable infectious diseases and 74,000 deaths caused by noncommunicable factors. Among these, there were 223,650 deaths attributed to COVID-19 at work. We calculated the rate of 584 DALYs per 100,000 workers, representing an 11% increase from the previous estimate of the global burden of work-related disabilities measured by DALYs. Conclusion: This is a first update since previous 2007 ILO estimates, which has now increased by 74% and covers most biological risks factors. However, it is important to note that there may be other diseases and deaths are missing from the data, which need to be included when new information becomes available. It is also worth mentioning that while deaths caused by major communicable diseases including COVID-19 are relatively rare within the working population, absences from work due to these diseases are likely to be very common within the active workforce.

• 한국문헌정보학회지
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• 제52권3호
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• pp.149-169
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• 2018

본 연구의 목적은 생명과학 분야에서 많은 연구개발비의 지원과 스타과학자로 구성된 S대학교 생명과학부에 소속된 교수진의 공동연구 유형별 연구 생산성과 인용 성과를 심도 있게 분석하고, 더 나아가 공동연구 유형별 인용 평가지표인 피인용빈도와 발간 당해 연도 피인용빈도, 그리고 논문 1편당 평균 피인용빈도에서 차이가 있는지 밝히는데 있다. 이를 위해 연구 대상자들이 발표한 Web of Science 등재 학술지 논문 1,135편(2004-2013년)을 공동연구 유형별로 발행패턴을 분석하였으며, 1,135편에 대한 13년간 피인용빈도(2004-2016년)를 수집하여 공동연구 유형별 피인용도를 분석하였다. 분석 결과, 네 가지 유형의 공동연구 중 국내 타 기관과 국제 공동연구 논문이 832편으로 73.3%를 차지하였으며, 공동연구 유형에 따른 논문 한 편당 평균 피인용빈도에서 통계적으로 유의한 차이가 있는 것으로 나타났다(F=4.830, $p=0.003^{**}$).

• Journal of Applied Biological Chemistry
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• 제59권3호
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• pp.207-213
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• 2016

Little information about the biological activities of Citrus limon (lemon) leaves has been reported, whereas the fruit of Citrus limon (lemon) has been well-documented to contain various pro-health bio-functional compounds. In the present study, the antiproliferative activities of the lemon leaves were evaluated using several cancer cell lines. From the n-hexane, chloroform, ethyl acetate, n-butanol, and water fractions of methanolic extract of the leaves, the chloroform fraction of lemon leaves (CFLL) showed the most potent antiproliferative activity in the AGS human gastric cancer cells. The current study demonstrates that CFLL induces apoptosis in AGS cells, as evidenced by an increase in apoptotic bodies, cell population in the sub-G1 phase, Bax/Bcl-2 ratio, and cleavage of poly (ADP-ribose) polymerase (PARP), caspase-3 and caspase-9. Compositional analysis of the CFLL using gas chromatography mass spectrometry (GC-MS) resulted in the identification of 27 compounds including trans, trans-farnesol (3.19 %), farnesol (3.26 %), vanillic acid (1.45 %), (-)-loliolide (5.24 %) and palmitic acid (6.96 %). Understanding the modes of action of these compounds individually and/or synergistically would provide useful information about their applications in cancer prevention and therapy.

• 복식
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• 제65권1호
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• pp.118-135
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• 2015

In recent years, smart clothing had been developed in order to better detect and monitor physical movement of the patient, so that such activities such as location identification and biometric recognition could be done. However, most of the sensing devices of smart clothing were limited to smart sensing sports clothing and the designs did not consider the physical characteristics and the behavior of the wearer. Therefore, this study aimed to create an open protection system by developing a wearable sensing device for health monitoring and location information. For this purpose, this study developed eleven types of wearable sensing design that could be commercially sold and worn by people who needed their biological information to be constantly monitored. The study conducted four tests in order to develop three types of sensing devices for various sensing wears. The purpose of this study was to expand the user rang of smart sensing wears, and provide a foundation for the development of distinctive wearable sensing devices reflecting the user. Furthermore, contribute to the design for the person subject to protection.

• Journal of Biosystems Engineering
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• 제26권6호
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• pp.545-552
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• 2001

Nondestructive methods such as ultrasonic and magnetic resonance imaging systems have many advantages but still much expensive. And they do not give exact color information and may miss some details. If it is allowed to destruct a biological object to obtain interior and exterior informations, 3D image visualization model from a series of sliced sectional images gives more useful information with relatively low cost. In this paper, a PC based automatic 3D visualization system is presented. The system is composed of three modules. The first module is the handling and image acquisition module. The handling module feeds and slices a cylindrical shape paraffin, which holds a biological object inside the paraffin. And the paraffin is kept being solid by cooling while being handled. The image acquisition modulo captures the sectional image of the object merged into the paraffin consecutively. The second one is the system control and interface module, which controls actuators for feeding, slicing, and image capturing. And the last one is the image processing and visualization module, which processes a series of acquired sectional images and generates a 3D volumetric model. To verify the condition for the uniform slicing, normal directional forces of the cutting edge according to the various cutting angles were measured using a strain gauge and the amount of the sliced chips were weighed and analyzed. Once the 3D model was constructed on the computer, user could manipulate it with various transformation methods such as translation, rotation, and scaling including arbitrary sectional view.

• Reproductive and Developmental Biology
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• 제33권2호
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• pp.77-83
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• 2009

Erythropoietin (EPO), a glycoprotein hormone produced from primarily cells of the peritubular capillary endothelium of the kidney, is responsible for the regulation of red blood cell production. We have been investigating the roles of glycosylation site added in the biosynthesis and function of recombinant protein. We constructed three EPO mutants ($\Delta$69, $\Delta$105 and $\Delta$69,105), containing an additional oligosaccharide chains. EPOWT and EPO$\Delta$69 were effectively expressed in transient and stably transfected CHO-K1 cell lines. But, it wasn't detected any protein in the culture medium of EPO$\Delta$105 and EPO$\Delta$69,105 mutants. The growth and differentiation of EPO-dependent human leukemic cell line (F36E) were used to measure the cytokine dependency and in vitro bioactivity of rec-hEPO. MTT assay values were increased by survival of F36E cells at 24h. To analysis biological activity in vivo, two groups of ICR-mice (7 weeks old) were injected subcutaneously with 10 IU per mice of rec-hEPO molecules on days 0 and 2. Red blood cell and hematocrit values were measured on 6 days after the first injection. The hematocrit values were remarkably increased in all treatment groups. The pharmacokinetic analysis was also affected in the mice injected with rec-hEPO molecules 2.5 IU by tail intravenous. Protein samples were detected by Western blotting. An EPO$\Delta$69 protein migrated as a broad band with an average apparent molecular and detected slightly high band. Enzymatic N-deglycosylation resulted in narrow band and was the same molecular size. The biological activity of EPO$\Delta$69 was enhanced to compare with wt-hEPO. The half-life was longer than wt-hEPO. The results suggest that hyperglycosyalted recombinant human erythropoietin (EPO$\Delta$69) may have important biological and therapeutic good points.

• 한국정보통신학회논문지
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• 제13권5호
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• pp.1008-1013
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• 2009

본 논문에서는 피실험자의 체온, 맥박, 호흡수, 혈압을 수집할 수 있는 무선 센서를 이용하여 수집된 생체 신호를 분석하는 시스템을 제안하였으며, 생체 신호를 분석하기 위하여 전문가 시스템을 사용하여 역전파 신경망을 설계 하였다. 제안된 시스템은 하드웨어 (UStar-2400 ISP, UStar-2400, Wireless sensors) 부분과 소프트웨어 부분 (Knowledge Base module, Inference Engine module, User Interface module)으로 구성되며 소프트웨어 부분은 HOST PC에 삽입된다. 그리고 시스템의 정확도를 개선하기 위하여 전방향 에러 정정 시스템(LDPC)을 구현하였다. 지식기반 모듈에서 100개의 데이터 집합을 사용하여 역전파 신경망을 훈련하였으며, 지식기반 모듈의 128개의 데이터 집합을 사용하여 실험한 결과 약 95%의 정확도를 가졌으며, 무선 센서를 사용하여 13명의 학생을 대상으로 실험한 결과 약 85%의 정확도를 획득하였다.

• Parasites, Hosts and Diseases
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• 제54권4호
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• pp.439-446
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• 2016

Toxoplasmosis is a protozoan disease that is caused by Toxoplasma gondii in livestock and humans. Due to its medical and veterinary importance, it is essential to study the seroprevalence of T. gondii infection among humans and animals in various parts of the world. The major objective of this study was to determine the seroprevalence and spatial distribution of toxoplasmosis in small ruminants (sheep and goats) of north-eastern region, Pakistan. A total of 1,000 animals comprising of sheep (n=470) and goats (n=530) were examined for T. gondii infection by using ELISA. An epidemiological data was collected in the form of questionnaire. A surface has been generated by using method of interpolation in Arc GIS with the help of IDW (inverse distance weight). The results showed higher seroprevalence of T. gondii in goats (42.8%) as compared to sheep (26.2%). The seroprevalence was higher in females as compared to males in all examined ruminants. Similarly, there is a wide variation in the seroprevalence of T. gondii in different breeds of sheep and goats showing higher seroprevalence in Teddy (52.8%) and Damani breed (34.5%) of goat and sheep's, respectively. The geographical and spatial distribution of T. gondii shows that it is widely distributed in different parts of the north-eastern region of Pakistan. Our results suggest widespread environmental contamination with T. gondii oocysts and that small ruminants could be a potentially important source of T. gondii infection if their infected meat is consumed undercooked.