• 제목/요약/키워드: Biological evaluation

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Evaluation of DNA Damage Using Microwave Dielectric Absorption Spectroscopy

  • Hirayama, Makoto;Matuo, Youichirou;Sunagawa, Takeyoshi;Izumi, Yoshinobu
    • Journal of Radiation Protection and Research
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    • 제41권4호
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    • pp.339-343
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    • 2016
  • Background: Evaluation of deoxyribonucleic acid (DNA)-strand break is important to elucidate the biological effect of ionizing radiations. The conventional methods for DNA-strand break evaluation have been achieved by Agarose gel electrophoresis and others using an electrical property of DNAs. Such kinds of DNA-strand break evaluation systems can estimate DNA-strand break, according to a molecular weight of DNAs. However, the conventional method needs pretreatment of the sample and a relatively long period for analysis. They do not have enough sensitivity to detect the strand break products in the low-dose region. Materials and Methods: The sample is water, methanol and plasmid DNA solution. The plasmid DNA pUC118 was multiplied by using Escherichia coli JM109 competent cells. The resonance frequency and Q-value were measured by means of microwave dielectric absorption spectroscopy. When a sample is located at a center of the electric field, resonance curve of the frequency that existed as a standing wave is disturbed. As a result, the perturbation effect to perform a resonance with different frequency is adopted. Results and Discussion: The resonance frequency shifted to higher frequency with an increase in a concentration of methanol as the model of the biological material, and the Q-value decreased. The absorption peak in microwave power spectrum of the double-strand break plasmid DNA shifted from the non-damaged plasmid DNA. Moreover, the sharpness of absorption peak changed resulting in change in Q-value. We confirmed that a resonance frequency shifted to higher frequency with an increase in concentration of the plasmid DNA. Conclusion: We developed a new technique for an evaluation of DNA damage. In this paper, we report the evaluation method of DNA damage using microwave dielectric absorption spectroscopy.

외상후 스트레스 장애 환자의 신경인지기능 (Neurocognitive Functions in Posttraumatic Stress Disorder)

  • 김선국;이강준;이승환;남민;정영조
    • 생물정신의학
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    • 제10권2호
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    • pp.147-158
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    • 2003
  • Objective:The differences of various neurocognitive functions, including attention, memory, motor function, and higher cognitive function were compared between PTSD patients and normal control subjects. Also, correlation with PTSD symptom severity and neurocognitive functions were evaluated between PTSD patients and normal control subjects. Method:We assessed the neurocognitive functions by computerized neurocognitive test(CNT) batteries. The visual continuous performance test(CPT) and digit span test, finger tapping test and Wisconsin card sorting test(WCST) were executed. The Impact of Event Scale-Revised(IES-R) was used in the evaluation of the severity of PTSD. Result:The PTSD patients showed significantly impaired neurocognitive performance in all of the items, compared with normal control subjects. The relation between impairment in neurocognitive functions and symptom severity showed significant correlations. Conclusion:These results imply that PTSD patients have impaired neurocognitive functions concerning with specific brain areas, especially the frontal area. For the thorough evaluation of further neurocognitive functions, more detailed evaluation items of neurocognitive functions and brain imaging studies are necessary in the future study.

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3차원 입체조형치료에 대한 간암의 선량분포와 정상조직손상확률의 평가 (Evaluation of DVH and NTCP in Hepatoma for 3D Conformal Radiation Therapy)

  • 정갑수;양한준;고신관
    • 대한방사선기술학회지:방사선기술과학
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    • 제20권2호
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    • pp.79-82
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    • 1997
  • Image-based three dimensional radiation treatment planning(3D RTP) has a potential of generating superior treatment plans. Advances in computer technology and software developments quickly make 3D RTP a feasible choice for routine clinical use. However, it has become clear that an evaluation of a 3D plan is more difficult than a 2D plan. A number of tools have been developed to facilitate the evaluation of 3D RTP both qualitatively and quantitatively. For example, beam's eye view(BEV) is one of the most powerful and time-saving method as a qualitative tools. Dose-volume histogram(DVH) has been proven to be one of the most valuable methods for a quantitative tools. But it has a limitation to evaluate several different plans for biological effects of the tissue and critical organ. Therefore, there is a strong interest in developing quantitative models which would predict the likely biological response of irradiated organs and tissues, such as tumor control probability(TCP) and normal tissue complication probability(NTCP). DVH and NTCP of hepatoma were evaluated for three dimensional conformal radiotherapy(3D CRT). Also, 3D RTP was analysed as a dose optimization based on beam arrangement and beam modulation.

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경관평가연구의 국내외 동향 (Domestic and Foreign Trends in the Study of the Landscape Evaluation)

  • 주신하;임승빈
    • 한국조경학회지
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    • 제28권2호
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    • pp.49-60
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    • 2000
  • The purpose of this study is to overview domestic and foreign trends in the study of the landscape evaluation through new framework of landscape evaluation studies. 108 studies on the landscape evaluation are summarized and categorized into theoretical studies, verification of theories, development of evaluation methods and applications in physical planning. Major theories in the landscape evaluation came from the psycho-physics, the evolutionary theory ann the cultural-learning theory, and were verified and applied into physical planning. Early experimental researches on landscape evaluation, based on psycho-physics, were focused on relatively simple responses to landscapes. But many studies have been gradually related to the evolutionary theory and the cultural learning theory, emphasizing biological and cultural effects on landscape evaluation. Especially, Appleton's Prospect-Refuge theory' and Kaplans' 'Information Processing model' have very strong influence in landscape evaluation. Relatively there have been many application researches in Korea, which tells there have been strong needs to solve pending practical problems caused by the rapid economic and social growth for several decades. Almost of applications in physical planning are focused on physical features of landscapes, but for more comprehensive landscape evaluation, many other factors such as cognitive and sociocultural variables should be integrated into the whole evaluation system. As a result of reviewing of landscape evaluation studies, I found the overall domestic and foreign trends and the necessity of more research on the applications in physical planning. Because this study mainly focused on academic researches, for more appropriate landscape evaluation and management there should be more practical researches including various approaches.

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하천환경 자연도의 평가지표 및 기준 연구 - 생물적 특성을 중심으로 (A study on indicator & criteria for assessment of river environmental naturalness -focused on biological characteristics)

  • 전승훈
    • 한국수자원학회논문집
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    • 제52권spc2호
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    • pp.765-776
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    • 2019
  • 본 연구는 하천복원사업의 전 과정에서 활용될 수 있는 법 제도적 지침과 기준을 제공하고 하천사업의 성과를 진단 평가할 수 있는 한국형 표준화된 하천환경 평가체계 구축과정의 일환으로서 하천생태계의 수변 및 수서환경을 대변할 수 있는 4개의 생물 분류군, 즉 식생과 조류, 그리고 저서 무척추동물과 어류의 평가지표 및 기준 등 평가체계를 구축하였다. 구체적으로 생물적 특성의 평가지표 및 기준을 정리하면, 식생의 경우 식생 다양도와 식생 복잡도, 그리고 식생 자연도 등 3가지 지수의 조합을 통한 하천 식생군집의 구조적 특성을 정량적으로 평가할 수 있도록 하였다. 저서 무척추동물과 어류, 그리고 조류의 경우도 선진 기법의 과학적 근거를 바탕으로 우리나라 하천특성에 적합하도록 생물적 자료의 평가등급 획정에 따른 정량적인 생물지수 평가법을 제안하였다. 아울러 하천환경 자연도의 한 부문인 생물적 특성의 평가를 위하여 이들 4개 생물분류군의 가중치를 적용한 종합 생물지수 및 평가등급화 방안을 제시하였으며, 이에 대한 시험하천의 적용결과에서도 생물분류군 간 비교적 일관성 있게 하천환경의 특성을 반영하고 있는 것으로 분석되었다.

QUALITY EVALUATION OF TECHNOLOGY OF AGRICULTURAL PRODUCTS

  • Chen, Pictiaw
    • 한국농업기계학회:학술대회논문집
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    • 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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    • pp.171-190
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    • 1996
  • Quality evaluation of agricultural products has been a subject of interest to many researches for many years. As a results, several nondestructive techniques for quality evaluation of agricultural products have been developed. These methods are based on the detection of various physical properties that correlate well with certain quality factors of the products. This paper presents an overview of various quality evaluation techniques that are based on one of the following properties : density, firmness , vibration characteristic , X-ray and gamma ray transmission, optical reflectance and transmission , electrical properties, aromatic volatile emission, and nuclear magnetic resonance (NMR). The sophistication of nondestructive methods has evolved rapidly with modern technologies. The use of various modern image acquisition techniques, such as solid state TV camera, line-scan camera, X-ray scanning , ultrasonic scanning and NMR imaging, in conjunction with image-processing te hniques has provided new opportunities for researchers to develop many new and improved techniques for nondestructive quality evaluation of agricultural products.

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모델식품을 이용한 맨드라미 적색색소의 식품학적 평가 (Evaluation of Red Pigment of Cockscomb Flower in Model Food Systems as a Natural Food Colorant)

  • 이상열;신용철;변시명;조재선;조숙자
    • 한국식품과학회지
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    • 제18권5호
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    • pp.389-392
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    • 1986
  • 맨드라미 붉은 색소의 식품학적 평가를 위하여 모델식품으로 젤리포(중간수분 식품), 캔디(고상식품) 및 샤베트(냉동식품)를 선택하여 맨드라미 붉은 색소로서 빨갛게 착색시켰다. 저장중 색도변화는 Hunter 색도계, Lovibond tintometer 및 분광광도로서 측정 분석하였고 아울러 색도관능 시험도 행하였다. 실험 결과로서 맨드라미 붉은 색소는 특정한 조건의 식품; 수분 활성도가 낮은 식품 및 냉동 식품에 성공적으로 활용할 수 있음을 알았다. 각 측정기기 사이에서 얻은 실험 결과는 밀접한 상관 관계가 존재하였다.

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