• Microbiology and Biotechnology Letters
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• v.50 no.2
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• pp.245-254
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• 2022

Cellulases are a group of biocatalyst enzymes that are capable of degrading cellulosic biomass present in the natural environment and produced by a large number of microorganisms, including bacteria and fungi, etc. In the current study, we isolated, screened and characterized cellulase-producing bacteria from soil. Three cellulose-degrading species were isolated based on clear zone using Congo red stain on carboxymethyl cellulose (CMC) agar plates. These bacterial isolates, named as HB2, HS5 and HS9, were subsequently characterized by morphological and biochemical tests as well as 16S rRNA gene sequencing. Based on 16S rRNA analysis, the bacterial isolates were identified as Bacillus cerus, Bacillus subtilis and Bacillus stratosphericus. Moreover, for maximum cellulase production, different growth parameters were optimized. Maximum optical density for growth was also noted at pH 7.0 for 48 h for all three isolates. Optical density was high for all three isolates using meat extract as a nitrogen source for 48 h. The pH profile of all three strains was quite similar but the maximum enzyme activity was observed at pH 7.0. Maximum cellulase production by all three bacterial isolates was noted when using lactose as a carbon rather than nitrogen and peptone. Further studies are needed for identification of new isolates in this region having maximum cellulolytic activity. Our findings indicate that this enzyme has various potential industrial applications.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.4
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• pp.554-557
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• 1999

Hatchery waste including infertile eggs, dead embryos in shell, dead or low grade chicks was cooked at $100^{\circ}C$ for 15 minutes and then oven dried and ground. Hatchery waste meal (HWM) thus prepared contained 44.25% protein, 4,573 kcal/kg gross energy, 3,600 kcal/kg metabolizable energy, 30% ether extract, 1.9% fibre, 14% ash, 9.8% nitrogen free extract, 7.26% Ca, 0.84%P, 1.86% lysine and 0.66% methionine with no Salmonella and E. coli. In biological evaluation trial, significantly higher weight gain was observed in ration containing 12% HWM compared with that containing similar amount of fish meal. Protein efficiency ratio on the two rations was 3.96 vs 2.85; protein digestibility, 86.02 vs 71.9; net protein utilization, 64.9 vs 42.37 and biological value, 75.37 vs 58.84, respectively, indicating better balance of amino acids in HWM compared with fish meal. Growth performance trial on broiler chicks also revealed better weight gain and feed efficiency on ration containing 12% HWM than that containing similar level of fish meal.

• Bulletin of the Korean Chemical Society
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• v.32 no.spc8
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• pp.3063-3073
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• 2011

The synthesis and biological evaluation of 1-heteroarylmethyl 1,4-diazepane derivatives as potential T-type calcium channel blockers is described. In this study, we have identified the compound 21i exhibiting the most potent T-type calcium channel blocking activity with $IC_{50}$ value of 0.20 ${\mu}M$, which is superior to that of mibefradil.

• Applied Biological Chemistry
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• v.46 no.1
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• pp.28-31
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• 2003

Soybean phytosterols have been known to have the effect of reducing the amount of cholesterol in blood. We isolated and analyzed soybean phytosterols from soybean scum. The functional mayonnaise was prepared by the addition of the different amounts of soybean phytosterols. To determine the desirable level of soybean phytosterols in mayonnaise, solubility, viscosity, the stability of emulsion and the sensory evaluation were measured. The most desirable level of soybean phytosterols was 0.5% in solubility, viscosity, stability of emulsion and the sensory evaluation.

• Environmental Engineering Research
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• v.16 no.2
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• pp.69-79
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• 2011

Rapid population growth and land use changes have severely degraded streams across the United States. In response, there has been a surge in the number of stream restoration projects, including stream restoration for mitigation purposes. Currently, most projects do not include evaluation and monitoring, which are critical in the success of stream restoration projects. The goal of this study is to review the current status of assessment methodologies and restoration approaches for streams in Virginia, with the aim of assisting the restoration community in making sound decisions. As part of the study, stream restoration projects data from a project in Fairfax County, Virginia was assessed. This review revealed that the stream assessment methodologies currently applied to restoration are visuallybased and do not include biological data collection and/or a method to incorporate watershed information. It was found from the case study that out of the twenty nine restoration projects that had occurred between 1995 and 2003 in Fairfax County, nineteen projects reported bank stabilization as a goal or the only goal, indicating an emphasis on a single physical component rather than on the overall ecological integrity of streams. It also turned out that only seven projects conducted any level of monitoring as part of the restoration, confirming the lack of evaluation and monitoring. However, Fairfax County has recently improved its stream restoration practices by developing and incorporating watershed management plans. This now provides one of the better cases that might be looked upon by stakeholders when planning future stream restoration projects.

• Applied Biological Chemistry
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• v.20 no.3
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• pp.279-284
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• 1977

Like that mentioned in the 2nd report, because of panel's sense of psychological and physiological responsibility caused by multi-samples, great errors in experimental results are expected. So as to cut down these errors, the new method called "Pair Comparision with Standard" that reduces test frequency and is superior in detecting power is designed, and its mathematical model is proposed. This paper suggests that this method can be used for screening test that, first of all, selects 4-5 of multi-samples and the most efficient sensory evaluation method in laboratorial quality study is that, after screening by this method, Trio Paired Comparison for the final justification is applicated.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.27-31
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• 2001

Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.357-362
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• 2006

A growth inhibition assay using Chlorella sp. AG 10002 based on the OECD 201 standard test procedure was applied to the toxicity testing of endosulfan and its reported metabolites. Comparison of dry cell weight, optical density (OD) at 680 nm, and chlorophyll a concentration indicated that optical density at 680 nm of culture broth is convenient, rapid, and accurate method for cell growth. In this microalgae system, the $IC_{50}$ values of endosulfan, endosulfan sulfate, endosulfan lactone, and endosulfan ether were determined as 9.45, 18.8, 18.2 and 37.5 mg/L, respectively. In a while, endosulfan diol did not show a significant toxicity up to 50 mg/L. Since endosulfan is liable at acidic or alkaline conditions, treatment of endosulfan in pH 3, 4, and 11 for 3 days resulted in reduced toxicity, as expected. These results suggested that the microalgae system is useful to evaluate various toxic chemicals and provide a new notion for bioremediation of endosulfan in aqueous systems.

• Journal of Korean Biological Nursing Science
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• v.3 no.2
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• pp.63-68
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• 2001

Increasing interest in computer-mediated learning technologies has prompted educators to incorporate them into many learning environments ; however, there is still little evaluative evidence to support their effectiveness. This report describes the development and evaluation of a web-based instructional program on basic nursing science for nursing students. Researcher-designed questionnaires were used to assess the characteristics of our students, and to solicit their ratings of the instructional program on ease of use, accuracy of content, clarity of content, interest, and convenience of the program, using 5-point Likert scales. The respondents indicated that the package was easy and convenient to use, with high technical quality, and of a level challenging to some but not all of the students. On-line quizzes were most highly rated. Also it was confirmed that frequent users of electronic bulletin board showed much higher achievement score than that of nonfrequent users. It was also found that the effect of cyber education was dependent on the active participation of the students. These data suggest that the use of web-based instructional program as a distance education strategy can be an effective method for nursing students and nurses.

• YAKHAK HOEJI
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• v.41 no.2
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• pp.187-194
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• 1997

For the development of antifungal agents, modification of naftifine which exhibits significant antimycotic activity was performed by replacing the naphthalene ring of it to hete ro cyclic rings such as morpholine. benzothiazole, piperidine and pyridine derivatives. The synthesized compounds were tested in vitro antifungal activity against five different fungi with naftifine as a comparative antimycotic molecule. From the biological evaluation two compounds, (E)-N-(3-phenyl-2-propenyl)-N-(4-piperidinylmethyl)amine(3d) and (E)-N-(3-phenyl-2-propenyl)-N-(3-pyridylmethyl)amine(3f) showed relatively noticeable activity(MIC=50${\mu}g$/ml). On the other hand, the other compounds had no activity.