• Applied Biological Chemistry
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• 제37권5호
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• pp.402-408
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• 1994

생리활성물질 monocerin의 구조상관활성을 조사하기 위해서 (8S, 9S, 11R)-(-)-monocerin과 (9S, 11R)-(+)-Fusarentin 4, 5-dimethyl ether를 합성하였는데, 합성은 benzylic anion과 유기용매에서 빵효모를 이용한 생체촉매반응으로 얻어진 (R)-aldehyde와의 축합에 의해 완성되었다. 합성되어진 화합물들은 병와 양상치를 대상으로 생리활성을 조사하였다. 특히, 벼의 생리활성검사에서 monocerin은 제2전엽보다 뿌리의 신장을 더 저해하였는데, fusarentin 4, 5-dimethyl ether는 제2전엽의 신장을 더 저해하였다. 생리활성시험의 결과, monocerin의 C환은 생리활성에 영향을 미치지 않는다는 사실이 예견되었고, 천연물의 입체구조적 배치가 비천연물의 입체구조적 배치보다 더 높은 생물활성을 나타내고 있다는 사실을 알 수 있었다.

• Mycobiology
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• 제47권1호
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• pp.50-58
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• 2019

Agarum clathratum, a brown macroalgae species, has recently become a serious environmental problem on the coasts of Korea. In an effort to solve this problem, fungal diversity associated with decaying A. clathratum was investigated and related ${\beta}$-glucosidase and endoglucanase activities were described. A total of 233 fungal strains were isolated from A. clathratum at 15 sites and identified 89 species based on morphology and a multigene analysis using the internal transcribed spacer region (ITS) and protein-coding genes including actin (act), ${\beta}$-tubulin (benA), calmodulin (CaM), and translation elongation factor (tef1). Acremonium, Corollospora, and Penicillium were the dominant genera, and Acremonium fuci and Corollospora gracilis were the dominant species. Fifty-one species exhibited cellulase activity, with A. fuci, Alfaria terrestris, Hypoxylon perforatum, P. madriti, and Pleosporales sp. Five showing the highest enzyme activities. Further enzyme quantification confirmed that these species had higher cellulase activity than P. crysogenum, a fungal species described in previous studies. This study lays the groundwork for bioremediation using fungi to remove decaying seaweed from populated areas and provides important background for potential industrial applications of environmentally friendly processes.

• Journal of Applied Biological Chemistry
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• 제63권4호
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• pp.297-303
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• 2020

Berries are rich sources of phenolic compounds, which are known to have health-promoting effects. In this study, phenolic compounds of seven popularly consumed berries were analyzed by HPLC-ESI-MS/MS. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and nitrite scavenging activities were investigated for comparison of the biological activity of the berry extracts. Chlorogenic acid was the major phenolic acid, which had the highest content in aronia, followed by blueberry. The rutin and isoquercetin contents were the highest in mulberry, followed by aronia, black raspberry, and blueberry. The anthocyanin content was the highest in black raspberry. Aronia showed the highest DPPH free radical and nitrite scavenging activities with the highest contents of total phenolics and proanthocyanidins.

• Journal of Applied Biological Chemistry
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• 제64권2호
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• pp.121-125
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• 2021

연잎(Nelumbo nucifera)의 60% (v/v) 에탄올 수용액 추출물의 항산화, 혈장 항응고 및 α-glucosidase 저해 효과를 in vitro에서 확인하였다. 연잎 추출물의 수율은 35.3±1.1%이었고, 폴리페놀 화합물 함량은 186.2±5.2 ㎍ gallic acid equivalents/mg으로 나타났다. 연잎 추출물은 농도에 비례하여 유리라디칼, 양이온라디칼 및 아질산염을 효과적으로 소거하였고 뛰어난 환원력을 보유하고 있었다. 또한 연잎 추출물은 농도 의존적으로 우수한 혈장 항응고 및 α-glucosidase 저해 활성을 나타내었다.

• 한국미생물·생명공학회지
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• 제12권4호
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• pp.305-309
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• 1984

섬유소 분해능이 있는 bacteria를 분리 동정하거나 유전자 재조합에 의한 형질 전환주중에서 이 분해능을 갖는 재조합주를 신속하고도 착실하게 분리해내는 일은, 섬유소 분해능을 가진 유전자를 복제 및 발현시키려는데 있어서 일차적인 조건이 된다. 한정된 양(0.005%)의 yeast ext를 함유한 기본배지에 0.5%의 CMC를 첨가 시킴으로써, 오직 섬유소 분해능이 있는 bacteria만을 분리할 수 있었고, 뒤이은 Congo red dye처리에 의해 세포벽 밖으로 분비 가능한 섬유소 분해효소를 생산하는 bacteria를 다시 구별해낼 수 있었다. 한편, 개발된 CMC 기본배지는 CMC대신 다른 탄소원 중합체를 대치시킴으로써, 다른 종류의 탄수화물 분해능을 갖는 bacteria도 쉽게 분리해 낼 수 있음을 알 수 있었다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.171-178
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• 2023

Morus alba, a popular medicinal plant belonging to the family Moraceae, has long been used commonly in traditional medicine and has various physiological activities, including antidiabetic, anti-microbial, diuretic, anti-oxidant, and anti-cancer activities. Morusinol was isolated from the root bark of M. alba; however, its biological effects have not yet been reported. Therefore, we examined the inhibitory effects of morusinol on human platelet aggregation, Ca²⁺ mobilization, and αIIb/β₃ activity. Our data showed that collagen-induced human platelet aggregation was inhibited by morusinol without cytotoxicity. In this study, we examined whether morusinol inhibits platelet aggregation through the regulation of integrin αIIb/β₃ and its associated signaling molecules. We observed that morusinol inhibited αIIb/β₃ activation by regulating vasodilator-stimulated phosphoprotein, phosphatidylinositol-3 kinase, Akt (protein kinase B), and glycogen synthase kinase-3α/β. These results show that morusinol inhibited fibronectin adhesion, fibrinogen binding, and clot retraction. Taken together, morusinol shows strong antiplatelet and anti-clot retraction effects and is a potential therapeutic drug candidate to prevent platelet-related thrombosis and cardiovascular disease.

• 대한화학회지
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• 제56권1호
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• pp.68-73
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• 2012

In the present study, we have synthesized novel Isopropyl 2-(4-substitutedbenzylidene)-5-methyl-3-oxo-7-phenyl-3,7-dihydro-2H-thiazolo[3,2-a]-pyrimidine-6-carboxylate derivatives (6a-j). Elemental analysis, IR, $^1H$ NMR and mass spectral data elucidated structure of newly synthesized compounds. The newly synthesized compounds were screened for antiinflammatory and anti microbial studies. Their biological activity data of the 10 compounds indicates that two compounds posses potent anti-inflammatory and five have antimicrobial activities.

• 약학회지
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• 제36권6호
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• pp.548-555
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• 1992

The biological activities of fruits of Poncirus trifoliata have been studied. 70% Ethanol extract of Ponciri Fructus was fractionated with ether, ethyl acetate and n-butanol by turns. The ether fraction showed antibacterial activity on Staphylococcus aureus and inhibited the contractability of the isolated mice ileum. The ethyl acetate and n-butanol fractions showed the anti-edematous effect on the carrageenin-induced edema of rat hindpaw. From the n-butanol fraction, one of the flavonoid glycoside was isolated and identified as poncirin. Ponciri Fructus contained about 6% poncirin which was 5,7-dihydroxy-4´-methoxy flavanone rhamnoglucoside and poncirin showed antiinflammatory activities. Poncirin was considered as the major effective component of Ponciri Fructus.

• Journal of Applied Biological Chemistry
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• 제51권3호
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• pp.111-116
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• 2008

Aralia elata seeds were successively extracted with water, methanol, ethanol, acetone and chloroform. The crude extracts were investigated for antioxidant and antidiabetic activities. The antioxidant properties of various extracts were evaluated by antioxidant tests, such as DPPH free radical-scavenging activity, hydroxyl radical-scavenging assay, metal-chelating activity, lipid peroxidation inhibition activity and reducing power assay. The 70% methanol extract exhibited the highest activity in the in vitro models of DPPH free radical-scavenging activity, metal-chelating activity, and reducing power assay. Acetone extract showed good effects on lipid peroxidation inhibition and hydroxyl radical-scavenging assay at a low concentration. In addition, the $\alpha$-glucosidase inhibition assay showed that 70% methanol extract had the highest activity. These results indicate the high possibility of using A. elata seeds for medical application due to their efficient antioxidant properties.

• Natural Product Sciences
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• 제17권1호
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• pp.5-9
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• 2011

An alkaloid, 3-methylcanthin-5, 6-dione, was isolated from the stem of Picrasma quassioides (D. Don) Benn. and characterized by comprehensive analyses of its 1D and 2D NMR spectra. It was also evaluated for its cytotoxic activity in vitro against three human cancer cell lines (MDA-MB-231, HT-29 and NCI-N87), using MTT assays. We found that 3-methylcanthin-5, 6-dione exhibited significant anti-inflammatory activity via inhibiting NO production induced in LPS-stimulated murine macrophage RAW264.7 cells. The antioxidant activity of 3-methylcanthin-5, 6-dione was measured by DPPH free radical scavenging assays, hydroxyl radical scavenging assays and reducing power assays. Our results showed that 3-methylcanthin-5, 6-dione has significant biological activities.