• Journal of Life Science
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• v.18 no.6
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• pp.871-877
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• 2008

Onions are considered to be a promising source of the alcoholic drink because they are rich in sugar, amino acids and various nutrients. To isolate strains of Saccharomyces cerevisiae producing ethanol of higher concentration, 19 strains were subjected to screening. Among them, the strain producing the highest concentration of ethanol was OJ-8 strain. Onion's oder was effectively removed by treatment for 30 min with 10% (w/v) charcoal against medium and then heat treatment of onion extract for 40 min at $100^{\circ}C$. The optimum conditions for alcoholic fermentation was investigated in medium containing the onion extract. The optimal conditions for ethanol production was obtained by standing culture for 5 days at $25^{\circ}C$ with 5% inoculum volume.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.115-121
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• 2001

Biological Treatment of Wastewater Containing Chlorinated Phenols by a Mixed Culture. Lee, Wan-Seok1, Sang-Wook Jung, Chan-Sun Park, Byung-Dae Yoon, Jang-Eok Kim\ and Hee-Mock Oh*. Environmental Bioresources Laboratory, Korea Research Institute of Biosicence and Biotechnology, Taejon, Korea, 1 Department of Agricultural Chemistry, Kyungpool< National University, Taegu, Korea - The biodegradation of chlorinated phenols in an artificial wastewater was investigated using a mixed culture. The mixed culture was composed of 8 microorganisms isolated from the soil contaminated with various chlorinated phenols. Pseudomonas sp. BM as a main constituent of a mixed culture was Gram-negative, catalase- and oxidase-positive, and rod-shaped, and did not grow at 41°C. It degraded 99% of initial 500 mg!1 of pentachlorophenol (PCP) in the minimal salts medium as a sole source of carbon and energy within 3 days. The degradation efficiency of Pseu.domon.as sp. BM was not affected by the other organic carbon and nitrogen compounds. Pseudomonas sp. BM was able to grow in a broad range of pH 5 - 8, and degrade 2,000 mg/1 PCP. In the experiment with an artificial wastewater containing chlorinated phenols, the degradation efficiency of the mixed culture was the range of 73% (2,4-dichlorophenol) -96% (2-chlorophenol) during an incubation of 7 days. In a continuous culture experiment, the degradation efficiency of mixed culture plus activated sludge was about 2 times higher than that of the control containing only activated sludge. These results indicate that it is possible to apply the mixed culture to other wastewaters containing chlorinated phenols. Key words: Biodegradation, chlorinated phenols, pentachlorophenol, Pseudomonas sp. BM

• Applied Biological Chemistry
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• v.51 no.3
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• pp.159-163
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• 2008

Xylogone sphaerospora ${\beta}$-mannanase was purified by Sephadex G-100 column chromatography. The specific activity of the purified enzyme was 8.44 units/ml protein, representing an 56.27-folds purification of the original crude extract. The final preparation thus obtained showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be 42kDa. Konjac glucomannan was hydrolyzed by the purified ${\beta}$-mannanase, and then the hydrolysates was separated by activated carbon column chromatography. The main hydrolysates were composed of D.P. (Degree of Polymerization) 3 and 4 glucomannooligosaccharides. For elucidate the structure of D.P 3 and 4 glucomannooligosaccharides, sequential enzymatic action was performed. D.P 3 and 4 were identified as M-G-M and M-M-G-M (G- and M- represent glucosidic and mannosidic link-ages). To investigate the effects of konjac glucomannooligosaccharides on in vitro growth of Bifido-bacterium longum, B. bifidum, B. infantis, B. adolescentis, B. animalis, B. auglutum and B. breve. Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon source such as D.P. 3 and D.P. 4 glucomannooligosaccharides, respectively. B. longum and B. bifidum grew up 3.9-fold and 2.8-fold more effectively by the treatment of D.P. 4 glucomannooligosaccharides, compared to those of standard MRS medium. Especially, D.P. 4 was more effective than D.P. 3 glucomannooligosaccharide on the growth of Bifidobacterium spp.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.237-241
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• 1999

Phenolic resin wastewater contained 41,000 mglI phenol, 2,800 mg/l fonualdehyde and various chlorinated phenolic compounds. Candida tropicalis PW-51 isolated [rom the natural enVlfooment was able to degrade 1,000 mg/l phenol in the presence of 100 mglI formaldehyde, but it took much time to degrade phenol with the increase of formaldehyde in phenolic resin wastewater. %en the phenolic resin wastewater was diluted to 1/40, the initial concentration of phenolic compounds (phenols) was 882 mglI and degraded to 81 mglI by C tfVpicalis PW-51 in batch culture. In a continuous biological treatment, the phenolic resin wastewater was diluted to 40 (745 mglI), 20 (1,356 mglI), or 10 (2,875 mglI) times. The removal efficiency of phenols in 1/40- and lI20-diluted phenolic resin wastewater was about 92%, but the phenols in 1!1O-diluted wastewater were not degraded. The remained phenols in wastewater were absorbed by a mixture of activated carbon and rice bran (1:1, v:v) in the process of absorption which was connected to the biological treatment. The total removal efficiency of phenols in 1!40~ and l/20-diluted phenolic resin wastewater was 99.9%.

• Journal of Korean Society of Water and Wastewater
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• v.28 no.4
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• pp.367-376
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• 2014

This study assessed the removal efficiency of NOM which is known as the precursors of DBPs in advanced water treatment using the ceramic membrane filtration, introduced the first in the nation at the Y water treatment plant (WTP). It is generally well-known that the removal of NOM by MF Membrane is very low in water treatment process. But, the result of investigation on removal efficiency of NOM in advanced water treatment using the ceramic membrane was different as follows. The removal rate of organic contaminant by the ceramic membrane advanced water treatment was determined to be 65.5% for the DOC, 85.8% for UV254, and 77 to 86% for DBPFP. The removal rate of pre-ozonation was found to be 6 to 15% more effective compared with the pre-chlorination. The removal rate of DOC and $UV_{254}$ in biological activated carbon(BAC) process was over 50% and 75%, respectively although the rate was decreased 10 ~ 20% according to analysis items in converting from GAC to BAC.

• Proceedings of the Membrane Society of Korea Conference
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• 1997.04a
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• pp.55-62
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• 1997

In Japan, the membrane filtration is becoming a common technology for municipal water supply system especially for small plant. 6 years before (1991), the national research project of membrane filtration for small plant has started. The project was named as "MAC 21", MEMBRANE AQUA CENTURY 21. In the project the Ministry of Health and Welfare, 8 universities and 18 water treatment plant companies have been involved. This was the first attempt to research a common theme in joint with government, universities and private companies. After three years, the guide line for membrane filtration application for small plant has been established. This has promoted to install some actual plant. And also, another joint research for "RESEARCH OF MEMBRANE FILTRATION FOR ADVANCED WATER TREATMENT" has started in 1994 and completed in March, 1997. The project was named as MAC21. In the former project the main objectives were removal of turbidity and bacteria from water. However, in new project the objective was establishment of the further advanced membrane filtration technology which would be applicable for trace chemical components removal such as tri-halo-methane pre-courser, agricultural chemicals removal, offensive smell and taste removal and virus removal. For the objectives, application of nanofiltration and hybrid-system, a combination of micro-filtration ultra-filtration with biological, ozone and activated carbon treatment process have been studied. In addition, application of membrane filtration for treatment of back-wash waste water originated from membrane filters and conventional sand filters has.been studied. At the end of March of this year, about 30 membrane filtration plants are actually supplying the water, the total treatment capacity is about 6,000 m$^{3}$/day and another 20 will be installed within one year.led within one year.

• Journal of Korean Society on Water Environment
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• v.25 no.5
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• pp.674-681
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• 2009

Characteristics of organic matters (OM) in wastewater and the removal efficiencies were investigated using the influent and the effluent samples collected from 21 wastewater treatment plants. The OM characteristics investigated included biodegradability, humic content, specific UV absorbance (SUVA), the distribution percentage of refractory OM (R-OM), and synchronous fluorescence spectra. The types of wastewater (sewage, livestock waste/night soils, industrial waste) were easily distinguished by comparing the synchronous fluorescence spectra of the influent wastewater. The prominent peak of protein-like fluorescence (PLF) was observed for livestock waste/night soils whereas sewage exhibited a unique fluorescence peak at a wavelength of 370 nm. Irrespective of the wastewater types, the distribution percentage of R-OM increased from the influent to the effluent. Livestock waste/night soils showed the highest removal efficiency among all the three types of wastewater. There was no statistical difference of the removal efficiency between a traditional activated sludge and biological advanced treatment processes. Removal efficiency based on dissolved organic carbon DOC presented good correlations with the distribution percentage of R-OM and fulvic-like fluorescence (FLF) of the influent. The prediction for DOC removal efficiency was improved by using multiple regression analyses based on some selected OM characteristics and mixed liquid suspended solid (MLSS).

• Journal of Korean Society of Water and Wastewater
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• v.9 no.2
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• pp.97-107
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• 1995

Today a conventional water treatment system has many problems. The ozone/GAC process, sometimes termed Biological Activated Carbon(BAC), appeared to be effective for the removal of soluble organic matters in the drinking water. The water quality of Nak-dong river in Pusan, generally shows BDOC 30-40% and NBDOC 60-70%. The pilot plant installed at the Duk-san water works that was been largest treatability(1,650,000ton/day) in Pusan. A experimental water in the pilot plant made use of the water after sand-filteration. Following results are drawn from this study. Initial adsorption velocity($DOC/DOC_o/T$) in the pure adsorption of GAG had a 0.0225, it's velocity changed to 0.006 after ozone added and the optimum ozone dose ranged of $1.4-2.0mgO_3/L$. A experimental water in the pilot plant composed with humic material(78%). Humic material composed with humic acid(20%) and fulvic acid(56%), and it's rate changed to 18 and 50% respectively after ozone added. DOC constantly decreased in the EBCTs and removal efficieny in the 15min of EBCT was 45-50%. It showed the largest removal rate of BDOC in the EBCT 5 and among the season, characteristics of removal varied. The HPC distributed over $10^6-10^7CFU/cm^3$ in the bed depth and among the season, distribution of HPC were differential.

• Applied Biological Chemistry
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• v.41 no.2
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• pp.181-186
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• 1998

The effects of sewage treatment on characteristics of sludge as a composting material were investigated for a year during the initial operation at the full-scale Chunan sewage treatment plant. Due to the shortage of design capacity of belt press, a sludge dewatering unit, non-volatile solids were recirculating and concentrating in the treatment plant, resulting in an increase of MLSS and a decrease in F/M ratio at the activated sludge system. Special attention is required for long term operations since the increase of non-volatile solids in the plant would deteriorates the treatment efficiency. The sewage sludge of the Chunan sewage treatment plant showed 79.5% of water content, 11.6% of organic content, and C/N ratio of 6.1, and contained As 1.8 mg/kg, Cd 27 mg/kg, Hg <0.1 mg/kg, Pb 54 mg/kg, T-Cr 370 mg/kg, and Cu 1,100mg/kg of heavy metals. In order to be used as raw material for optimum composting, the sewage sludge requires bulking agents for moistrure/porosity control and a carbon source for adjusting C/N ratio. However, the sewage sludge is not adequate as a soil conditioner after composing due to a high content of heavy metals. If the sewage sludge has to he used as a soil conditioner after composting, it as required to identify and remove tire industrial wastewater portions in tire influent of the plant since heavy metals in the influent were mostly concentrated in dewatered sludge.

• Journal of Korean Society of Water and Wastewater
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• v.25 no.4
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• pp.605-610
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• 2011

The objectives of this study are to investigate a bromate behaviour in the processes of advanced water treatment system (AWTS: preozonation, coagulator-settler, rapid sand filter, postozonation, biological activated carbon (BAC) beds) and to investigate the effects of ozonation, pH and ammonia nitrogen on bromate (${BrO^-}_3$) formation. As a result, $BrO_3$ was not detected in the processes of the AWTS without ozonation, while it was detected in a preozonated and postozonated water. For $BrO_3$ formation during June to November, the $BrO_3$ concentration of <9.4${\mu}g/L$ was observed in postozonated water, while it was reduced to about 46% by BAC beds. When applied ozone dosage and ozone contact time for influent with $Br^-$ of <0.3mg/L were 0.5-2.0mg/L.min and 10 min., $BrO_3$ concentration increased with increasing ozone dosage. Longer contact time and lower ozone level also was needed to inhibit the formation of $BrO_3$. At ozone dosage of 1.4 mg/L.min, the formation rate of $BrO_3$ increased with increase of pH value. When $NH_4-N$ concentration increased from 0.1mg/L to 0.4mg/L, $BrO_3$ concentration decreased to about 38%. These results revealed that $BrO_3$ concentration increased with increasing Br level, ozone dosage, and pH value, while it decreased with increase of $NH_4-N$ concentration.