• Title/Summary/Keyword: Biological Markers

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RAG-1 and IgM Genes, Markers for Early Development of the Immune System in Olive Flounder, Paralichthys olivaceus

  • Lee, Jang-Wook;Yang, Hyun;Noh, Jae Koo;Kim, Hyun Chul;Park, Choul-Ji;Park, Jong-Won;Hwang, In Joon;Kim, Sung Yeon;Lee, Jeong-Ho
    • Development and Reproduction
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    • v.18 no.2
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    • pp.99-106
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    • 2014
  • Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immune system in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulin M (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, the expression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) and used as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dph and remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its function around 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such as spleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 and IgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biological significance of such a phenomenon remains to be investigated.

Region-Based 3D Image Registration Technique for TKR (전슬관절치환술을 위한 3차원 영역기반 영상정합 기술)

  • Key, J.H.;Seo, D.C.;Park, H.S.;Youn, I.C.;Lee, M.K.;Yoo, S.K.;Choi, K.W.
    • Journal of Biomedical Engineering Research
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    • v.27 no.6
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    • pp.392-401
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    • 2006
  • Image Guided Surgery (IGS) system which has variously tried in medical engineering fields is able to give a surgeon objective information of operation process like decision making and surgical planning. This information is displayed through 3D images which are acquired from image modalities like CT and MRI for pre-operation. The technique of image registration is necessary to construct IGS system. Image registration means that 3D model and the object operated by a surgeon are matched on the common frame. Major techniques of registration in IGS system have been used by recognizing fiducial markers placed on the object. However, this method has been criticized due to additional trauma, its invasive protocol inserting fiducial markers in patient's bone and generating noise data when 2D slice images are acquired by image modality because many markers are made of metal. Therefore, this paper developed shape-based registration technique to improve the limitation of fiducial marker based IGS system. Iterative Closest Points (ICP) algorithm was used to match corresponding points and quaternion based rotation and translation transformation using closed form solution applied to find the optimized cost function of transformation. we assumed that this algorithm were used in Total Knee replacement (TKR) operation. Accordingly, we have developed region-based 3D registration technique based on anatomical landmarks and this registration algorithm was evaluated in a femur model. It was found that region-based algorithm can improve the accuracy in 3D registration.

Expression of Ki-67, p53 and VEGF in Pediatric Neuroblastoma

  • Gheytanchi, Elmira;Mehrazma, Mitra;Madjd, Zahra
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.7
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    • pp.3065-3070
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    • 2014
  • Background: Neuroblastoma (NB), is a neuroectodermal tumor derived from neural crest cells, and it is the second most common pediatric malignant tumor. The biological and clinical behavior of NB is very heterogeneous. This study was conducted to evaluate the expression of Ki-67, p53 and VEGF markers in tissues obtained from NB patients with different histologic types and stage. Materials and Methods: Tissue microarray (TMA) blocks were constructed from paraffin blocks of the NB tissues. Immunohistochemical staining was performed on TMA sections to detect the expression of Ki-67, p53 and VEGF markers. The association between the expression of these markers and clinicopathological parameters were then analyzed. Results: We had 18 patients with NB, one patient with ganglioneuroblastoma (GNB) and one with ganglioneuroma. Ki-67 was expressed in 13 (65%) tumors, and negatively correlated with age, prognosis, histologic type and stage of NB (all p<0.05). High and moderate expression of VEGF was found in 5% (1/20) and 65% (13/20) of the tumors, respectively; and it was positively correlated with age, prognosis and histologic types (all p<0.05) and negatively correlated with MKI (mitosis-karyorrhexis index). p53 expression was observed in 10% (2/20) of the tumors, which showed a relative correlation with MKI (p value=0.07). Conclusions: VEGF as a candidate for anti-angiogenic targeted therapy was correlated with the development and progression of NB; therefore, VEGF along with Ki-67 can serve as a valuable marker for the prognosis of this tumor type.

Development of intracellular organelle markers using modified glycolipid-binding peptides in mammalian cells (세포내 특정 소기관 타기팅 마커 개발을 위한 당지질-결합 펩타이드 변형 및 세포내 타기팅 분석)

  • Jun, Yong-Woo;Lee, Jin-A;Jang, Deok-Jin
    • Analytical Science and Technology
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    • v.28 no.1
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    • pp.65-71
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    • 2015
  • Intracellular organelles in eukaryotic cells play important roles in many cellular functions. Intracellular trafficking of many proteins to specific intracellular organelles is tightly regulated by various mechanisms in cells. Therefore, elucidating the targeting mechanism of novel markers for intracellular organelles is important for cellular physiology and pathology. In this study, we tried to identify the peptides which could bind to specific glycolipid in cellular membrane using GFP-fused glycolipid-binding peptides, and analyzed their cellular localization. As a result, we could identify mitochondria-, Golgi- or plasma membrane-targeting peptides. Furthermore, we found that the plasma membrane-targeting peptide was localized to the plasma membrane via electrostatic interactions. Thus, our results suggest that various glycolipid-binding peptides could be used as intracellular organelles markers.

Effects of Mulberry-Leaf Powder Tofu Consumption on Carpal Bone Mineral Density, Biochemical Bone Turnover Markers and Serum Lipid Profiles in Smoking Male Adults Living in Choongnam (뽕잎 분말 첨가 두부 섭취가 충남 일부 지역에 거주하는 흡연 남자 성인의 손목 골밀도, 생화학 골대사 지표 및 혈청 지질 성상에 미친 영향)

  • Kim, Ae-Jung;Kim, Myung-Hwan;Chung, Kun-Sub
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.1
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    • pp.1-10
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    • 2007
  • The effects of mulberry-leaf powder Tofu(MPT) on anthropometric measurements, including bone mineral density(BMD) in the right carpus, biochemical bone turnover markers, serum levels of lipids and macrominerals, were investigated in 30 smoking male adults who lived in Choongnam were given MPT(100 g/day) for 4 weeks. The average ages, number of smoked cigarettes and packyear were 22.38 years, 15.12/day and 3.54 years, respectively. The nutrient contents per 100 g MPT were 86.10 kcal energy, 8.98 g protein, 0.53 mg fiber, 211.33 mg Ca and 1.59 g fat. Anthropometric measurements, including dietary intake using the 24-hours recall method, carpal BMD using DEXA, serum levels of protein, albumin and glucose, lipid profiles (cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol) with Al(atherosclerosis index), HTR, CRF, LHBt, some biomarkers of BMD(serum alkaline phosphatase activity, osteocalcin, urinary DPD), and serum macrominerals(Ca, Ca/P ratio, Mg) and Pb were analyzed before and after consumption of MPT. After MPT consumption, dietary intakes of plant protein, total Ca and plant Ca increased significantly, but there were no significant differences in anthropometric measurements, BMD with bone metabolism markers, serum levels of protein, albumin or glucose, lipid profiles with AI, HTR, LHR and CRF.

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Image-guided Surgery System Using the Stereo Matching Method (스테레오 매칭 기법을 이용한 영상유도시술 시스템)

  • 강현수;이호진;문찬홍;문원진;김형진;최근호;함영국;이수열;변홍식
    • Journal of Biomedical Engineering Research
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    • v.24 no.4
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    • pp.339-346
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    • 2003
  • MRI provides anatomical structure information with superb spatial resolution that can be utilized in clinical surgeries. Advanced image processing techniques in conjunction with the MRI-guided surgery is expected to be of great importance in brain surgeries in the near future. In this paper, we introduce an image-guided surgery technique using the stereo matching method. To perform image-guided biopsy operations, we made MRI markers, camera markers and a detection probe marker. To evaluate the accuracy of the image-guided system. we made a silicone phantom. Using the phantom and markers, we have performed MRI-guided experiments with a 1.5 Tesla MRI system. It has been verified from phantom experiments that our system has a positioning error less than 1.5%. Compared with other image guided surgery system, our system shows better positioning accuracy.

Tumorsphere formation and cancer stem cell characterization of REM134 canine mammary carcinoma cells (개 REM134 유선종양세포의 sphere 형성을 통한 암 줄기세포 특성 분석)

  • Byeon, Jeong Su;Lee, Jienny;Jeong, Da-Un;Gu, Na-Yeon;Cho, In-Soo;Cha, Sang-Ho
    • Korean Journal of Veterinary Research
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    • v.58 no.4
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    • pp.201-209
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    • 2018
  • Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties.

Development of EST-SSRs and Assessment of Genetic Diversity in Germplasm of the Finger Millet, Eleusine coracana (L.) Gaertn.

  • Wang, Xiaohan;Lee, Myung Chul;Choi, Yu-Mi;Kim, Seong-Hoon;Han, Seahee;Desta, Kebede Taye;Yoon, Hye-myeong;Lee, Yoonjung;Oh, Miae;Yi, Jung Yoon;Shin, Myoung-Jae;Kim, Kyung-Min
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.443-451
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    • 2021
  • Finger millet (Eleusine coracana) is widely cultivated in tropical regions worldwide owing to its high nutritional value. Finger millet is more tolerant against biotic and abiotic stresses such as pests, drought, and salt than other millet crops; therefore, it was proposed as a candidate crop to adapt to climate change in Korea. In 2019, we used expressed sequence tag simple sequence repeat (EST-SSR) markers to evaluate the genetic diversity and structure of 102 finger millet accessions from two geographical regions (Africa and South Asia) to identify appropriate accessions and enhance crop diversity in Korea. In total, 40 primers produced 116 alleles, ranging in size from 135 to 457 bp, with a mean polymorphism information content (PIC) of 0.18225. Polymorphism was detected among the 40 primers, and 13 primers were found to have PIC values > 0.3. Principal coordinate and phylogenetic analyses, based on the combined data of both markers, grouped the finger millet accessions according to their respective collection areas.Therefore, the 102 accessions were classified into two groups, one from Asia and the other from Africa. We have conducted an in-depth study on the finger millet landrace pedigree. By sorting out and using the molecular characteristics of each pedigree, it will be useful for the management and accession identification of the plant resource. The novel SSR markers developed in this study will aid in future genetic analyses of E. coracana.

Lactobacillus rhamnosus JY02 Ameliorates Sarcopenia by Anti-Atrophic Effects in a Dexamethasone-Induced Cellular and Murine Model

  • Juyeon Lee;Minkyoung Kang;Jiseon Yoo;Sujeong Lee;Minji Kang;Bohyun Yun;Jong Nam Kim;Hyoungsun Moon;Yihyung Chung;Sangnam Oh
    • Journal of Microbiology and Biotechnology
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    • v.33 no.7
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    • pp.915-925
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    • 2023
  • Sarcopenia is defined as loss of muscle mass and strength due to aging. Recent studies show that sarcopenia may improve via the gut-muscle axis, suggesting that gut health may affect muscle phenotypes. In this study, we aimed to investigate the ability of Lactobacillus rhamnosus JY02 as a probiotic strain isolated from kimchi to alleviate sarcopenia. L. rhamnosus JY02-conditioned medium (CM) reduced dexamethasone (DEX)-induced myotube diameter atrophy and expression of muscle degradation markers (MuRF1 and atrogin-1) in C2C12 cells. The amelioration of sarcopenia was investigated by measuring body composition (lean mass), hand grip strength, myofibril size (using histological analysis), and mRNA and protein expression of muscle-related factors in a DEX-induced mouse model. The results of these analyses showed that L. rhamnosus JY02 supplementation promoted the production of muscle-enhancement markers (MHC Iβ, MHC IIα, and Myo-D) and reduced both the production of muscle degradation markers and the symptoms of muscle atrophy (loss of lean mass and muscle strength). We also found decreased levels of pro-inflammatory cytokines (IL-6, IFN- γ) and increased levels of anti-inflammatory cytokines (IL-10) in the serum of DEX+JY02-administered mice compared to those in DEX-treated mice. Overall, these results suggest that L. rhamnosus JY02 is a potent probiotic supplement that prevents sarcopenia by suppressing muscle atrophy.

Gene Discovery Analysis from Mouse Embryonic Stem Cells Based on Time Course Microarray Data

  • Suh, Young Ju;Cho, Sun A;Shim, Jung Hee;Yook, Yeon Joo;Yoo, Kyung Hyun;Kim, Jung Hee;Park, Eun Young;Noh, Ji Yeun;Lee, Seong Ho;Yang, Moon Hee;Jeong, Hyo Seok;Park, Jong Hoon
    • Molecules and Cells
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    • v.26 no.4
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    • pp.338-343
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    • 2008
  • An embryonic stem cell is a powerful tool for investigation of early development in vitro. The study of embryonic stem cell mediated neuronal differentiation allows for improved understanding of the mechanisms involved in embryonic neuronal development. We investigated expression profile changes using time course cDNA microarray to identify clues for the signaling network of neuronal differentiation. For the short time course microarray data, pattern analysis based on the quadratic regression method is an effective approach for identification and classification of a variety of expressed genes that have biological relevance. We studied the expression patterns, at each of 5 stages, after neuronal induction at the mRNA level of embryonic stem cells using the quadratic regression method for pattern analysis. As a result, a total of 316 genes (3.1%) including 166 (1.7%) informative genes in 8 possible expression patterns were identified by pattern analysis. Among the selected genes associated with neurological system, all three genes showing linearly increasing pattern over time, and one gene showing decreasing pattern over time, were verified by RT-PCR. Therefore, an increase in gene expression over time, in a linear pattern, may be associated with embryonic development. The genes: Tcfap2c, Ttr, Wnt3a, Btg2 and Foxk1 detected by pattern analysis, and verified by RT-PCR simultaneously, may be candidate markers associated with the development of the nervous system. Our study shows that pattern analysis, using the quadratic regression method, is very useful for investigation of time course cDNA microarray data. The pattern analysis used in this study has biological significance for the study of embryonic stem cells.