• Korean Journal Plant Pathology
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• v.13 no.2
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• pp.125-128
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• 1997

Reactions of Pseudomonas syringae pv. actinidiae to 95 carbon sources in a 96-well microplate (BiOLOG GN MicroPlateTM) were investigated. The bacterium used 9 carbon sources such as D-mannitol, sucrose, etc., but did not use 62 carbon sources such as $\alpha$-cyclodextrin, dextrin, etc. Based on the reactions, a user data base for identification of P. syringae pv. actinidiae was constructed in Biolog program (BiOLOG MicroLogTM 2 system). P. syringae pv. actinidiae isolates collected from kiwifruits could be identified automatically with high similarity using the user data base, which could diagnose rapidly and easily whether the tree was infected with bacterial canker or not.

• Korean Journal Plant Pathology
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• v.14 no.4
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• pp.361-363
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• 1998

Occurrence of soft rots was observed on celery that was massively grown in Pyungchang, Kangwon-Do, Korea. Soft rot symptom appeared first on the lower parts of the celery which eventually extended into whole aboveground parts of it. The casual organism isolated from the infected lesions was identified as Erwinia carotovora subsp. carotovora based on the physiological and chemical characteristics, and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes the bacterial soft rot disease on celery in Korea.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.548-550
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• 1998

Bacterial soft rot caused damage to beets massively grown in Pyungchang, Kangwon province, Korea. The affected roots and stems became cream colored and slimy, and turned black, causing the plants to become wilt and die. The casual organism isolated from the infected plants was identified as Erinia carotovora subsp. carotovora based on physiological and biochemical characteristic, and the results of the Biolog program (Biolog Inc., U.S.A.). E. carotovora subsp. carotovora is the first discribed bacterium which causes bacterial soft rot on beet in Korea.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.116-119
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• 1995

Occurrence of soft rots was observed on chicory that was massively grown in-In-jae, Kangwon-Do, Korea. At first, a creamy lesion was appeared on the chicory root, which was enlarged slowly in diameter and in depth. The affected root area became soft and mushy. This eventually resulted in wilting and death of the aboveground parts of the chicory. The causal organism isolated from the lesions was identified as Erwinia carotovora subsp. carotovora based on the physiological and chemical characteristics, and on the results of the Biolog Program (Biolog Inc. U.S.A.). Since E. carotovora subsp. carotovora is the first described bacterium that causes soft rot on chicory in Korea, we proposr to name the chicory disease caused by E. carotovora subsp. carotovora as "bacterial soft rot of chicory".

• Korean Journal Plant Pathology
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• v.14 no.4
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• pp.364-366
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• 1998

Bacterial soft rot was occurrenced on stems of cauliflower at a trial field of Alpine Agricultural Experiment Station, Kangwon-Do, Korea. the symptoms began as a small water-soaked lesion, which enlarged rapidly in diameter. The tissue within the affected region became slimy, disintegrating into a mushy mass of disorganized cells. The causal organism was isolated from the diseases lesions and was identified as Erwinia carotovora subsp. carotovora based on the morphological, physiological and chemical characteristics , and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes bacterial soft rot on cauliflower in Korea.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.555-557
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• 1998

Occurrence of soft rots was observed on wasabi (Wasabia japonica Matsum) grown in Chuncheon and Pyengchang Kangwon province, Korea. The symptoms appeared on the wasabi root, which became mushy and black. This eventually resulted in wilting and death of the aboveground parts of the wasabi. The causal organism was isolated from the infected lesions and was identified as Erwinia carotovora subsp. carotovora based on the morphological, physiological and biochemical characteristics, and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes bacterial soft rot on wasabi in Korea.

• Korean Journal Plant Pathology
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• v.14 no.6
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• pp.738-740
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• 1998

Bacterial soft rot was occurred on fruit of pepper that was grown in Chunceon, Kangwon province, Korea. The symptoms began as a small hole at 5 mm diameter, which made injury by a tobacco bud worm (Heliothis assulta). The affected fruit became soft and produced offensive odor. The causal organism was isolated from the diseased fruit and was identified as Erwinia carotovora subsp. carotovora based on the morphological, physiological and biochemical characteristics, and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes bacterial soft rot on pepper in Korea.

• Korean Journal Plant Pathology
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• v.13 no.1
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• pp.13-17
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• 1997

A causal agent of bacterial soft rot occurring in soybean sprout cultivation in Korea was isolated and identified, and its incidence in several sprout-soybean cultivars was examined. Infected soybean seeds became light brown and whitish, and could not germinate until 3 days after seeding, accompanying rotting of soybean seeds and sprouts. The causal organism isolated from the rotten seeds and sprouts was identified as Erwinia carotovora subsp. carotovora on the basis of its pathogenicity, morphological and physiological characteristics and the results of the Biolog GN microplate test program. The bacterial soft rot by E. c. subsp. carotovora was firstly described in soybean sprout in Korea, and we name it“the bacterial soft rot of soybean sprout”. The disease occurred more frequently in Nam-hae and Fu-reun sprout-soybean cultivars than in Eun-ha, So-baek, and Ik-san cultivars.

• Journal of The Korean Association For Science Education
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• v.34 no.8
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• pp.767-778
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• 2014

Many biologists and various educational associations define evolution as a unifying theme as well as a central idea in biology. In this study, teacher education programs were developed as 18-hour courses on understanding the unified role and significance of evolution in biology, and their effectiveness was tested. As factors for the program content, enhancement of the acceptance of evolution, reinforcement of the conception of evolutionary theory, and recognizing and practicing evolution as the unifying nature of evolution were considered. Hands-on activities, self- and peer-evaluation, and projects based on the small teams including frequent questioning and feedback by instructors were considered methodological factors. Six in-service biology teachers and seven pre-service teachers took part in the programs of the graduate school of H university to testing their effectiveness. They were effective in improving the acceptance of evolution, the understanding of evolutionary mechanism, the perception on the unified role of evolution. The programs also induced a positive change in self-evaluation for knowledge of evolution and perception on importance of evolution in biology. Subsequent studies on whether the changed perceptions of the teachers who participated in the programs reflect on their biology classes in secondary schools after finishing the graduate program are required.

• The Plant Pathology Journal
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• v.27 no.4
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• pp.333-341
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• 2011

Two antifungal bacteria were selected from forest soils during the screening of microorganisms antagonistic to Cylindrocarpon destructans, a cause of ginseng root rot. The antifungal bacteria were identified as Bacillus subtilis (I4) and B. amyloliquefaciens (yD16) based on physiological and cultural characteristics, the Biolog program, and 16S rRNA gene sequencing analyses. Antagonistic activity of both bacterial isolates to C. destructans increased with increasing temperature. More rapid starch hydrolytic activity of the bacteria was seen on starch agar at higher temperatures than at lower temperatures, and in the higher density inoculum treatment than in the lower density inoculum treatment. The bacterial isolates failed to colonize ginseng root the root tissues inoculated with the bacteria alone at an inoculum density of $1{\times}10^6$ cfu/ml, but succeeded in colonizing the root tissues co-inoculated with the bacteria and C. destructans. Scanning electron microscopy showed that the pathogen was damaged by the low-density inoculum treatment with the bacterial isolates as much as by the high-density inoculum treatment. Both bacterial isolates were more effective in reducing root rot when they were treated at a concentration of $1{\times}10^6$ cfu/ml than at $1{\times}10^8$ cfu/ml. Also, only the former treatment induced prominent wound periderm formation, related to structural defense against pathogen infection. The results suggest that the bacterial antagonists may have high potential as biocontrol agents against ginseng root rot at relatively low-inoculum concentrations.