• Korean Journal of Food Science and Technology
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• v.53 no.1
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• pp.19-28
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• 2021

Vitamin E and K are essential micronutrients required by our body in small amounts for proper metabolic functions; however, the content of vitamin E and K commonly consumed in foods has not been comprehensively defined. In this study, the contents of vitamins E and K in sea algae and vegetables were analyzed and the analytical methods were validated. The α-tocopherol equivalent (α-TE) and vitamin K1 content in sea algae ranged from 0.15 to 1.14 mg/100 g and from 11.91 to 1,629.5 ㎍/100 g, respectively. In addition, α-TE and vitamin K1 of vegetables were detected in the range of 0.02-2.48 mg/100 g and 16.15-979.60 ㎍/100 g, respectively. In particular, β- and γ-tocopherol and α- and β-tocotrienol were detected in several vegetables. The analytical methods were accurate and reproducible. These results provide reliable data on the vitamin E and K contents of foods consumed in Korea for the development of National Standard Food Composition Database.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.2
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• pp.69-75
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• 2004

In recent years, the importance of proteomic works, such as protein expression, detection and identification, has grown in the fields of proteomic and diagnostic research. This is because complete genome sequences of humans, and other organisms, progress as cellular processing and controlling are performed by proteins as well as DNA or RNA. However, conventional I protein analyses are time-consuming; therefore, high throughput protein analysis methods, which allow fast, direct and quantitative detection, are needed. These are so-called protein microarrays or protein chips, which have been developed to fulfill the need for high-throughput protein analyses. Although protein arrays are still in their infancy, technical development in immobilizing proteins in their native conformation on arrays, and the development of more sensitive detection methods, will facilitate the rapid deployment of protein arrays as high-throughput protein assay tools in proteomics and diagnostics. This review summarizes the basic technologies that are needed in the fabrication of protein arrays and their recent applications.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.6
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• pp.519-522
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• 2004

In the course of anaerobic storage of excess sludge, odors due to chemicals such as hydrogen sulfide are produced. These odors cause many problems. Many methods have been developed to eliminate odors, but all current methods are not only costly, but also largely inef­fective. In this paper, we investigate the process of transformation of sludge microorganism cul­tures through intense aeration under nutrient-poor conditions, in terms of the selective adjust­ment and control of microorganism culture. The aerated sludge is subsequently returned to the adjusting pool, where the microorganisms inhibit odors, thus the excess sludge itself will act as an odor inhibitor. The process can be verified in terms of viability, in that the degradation capac­ity of the sludge was maintained after the intensely-aerated sludge was returned to the treat­ment system.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.264-265
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• 2000

Helicobacter pylori(H. pylori) is the causative agent of chronic gastritis and the single most important factor in peptic ulcer disease, however, the pathogenetic mechanisms underlying H, pylori infection are not well understood. Futhermore, there is a strong association between H. pylori infection and gastric cancer. Various diagnostic methods for detecting H. pylori infection are available. These can be divided into invasive methods, requiring endoscopy, and non-invasive tests, mainly 13C-urea breath tests and serologic detection of antibodies. Rapid urease test is the most recommendable endoscopic test for the diagnosis of H. pylori infection, presently. CLO test kit is the represent of rapid urease test kits. The principles of CLO test kit is that hydrolysis of urea by urease Is detected by a dye indicators showing a color change. Our device is used same principle but we improved the reaction time is more faster and positive color change is more distinctive from the color of the negative specimen. So, this kit is more reliable because it response faster and accuracy.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.23 no.4
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• pp.361-367
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• 2014

Ultra-precision positioning plays a crucial role in emerging technologies such as electronics, bioengineering, optics, and various nanofabrication technologies. As a result, various nanopositioning methods have been presented. In particular, nanopositioning using a flexure mechanism and piezo-electric actuator is one of the most valuable methods because of its friction-free motion and subnanometer-scale motion resolution. In this study, a rotational nanoactuator based on a right-circular flexure mechanism and piezo-electric actuator was developed through a consideration of the kinematics and structural deformation. An experimental setup was constructed to verify the performance expectation. Consequently, it was demonstrated that the developed system had a maximum rotational angle of about 0.01 rad, as well as sufficient linearity with respect to the input voltage.

• International Journal of Precision Engineering and Manufacturing
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• v.7 no.2
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• pp.3-7
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• 2006

We propose a new concept of transmission-tribology design based on the investigation of development and existing problems of the gear drive and tribology. The content of transmission-tribology and transmission-tribology design is probed. Some effective methods of transmission-tribology design is probed. Some effective methods of transmission-tribology design are put forward. Partial achievements in this research are introduced tersely. Mechanical transmission, which is represented by the gear drive, and tribology are two main branches of mechanical engineering. They have their own definite research field. In recent years, we have been doing our utmost to suggest combining mechanical transmission-tribology and transmission-tribology design. Partial achievements in this research area are obtained.

• KSBB Journal
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• v.19 no.6 s.89
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• pp.421-426
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• 2004

The extraction and separation of isoflavones from Korean soybean were peformed by various mechanical and chemical extraction methods. They included solvent extraction, stirring, supersonification and sub/supercritical water extraction. From the experimental results of the variation of solvent extraction by change in composition, the increase in extraction of a specific compound by stirring or supersonic energy, and the application of supercritical fluid with superior solvating power over solvents, the sonification was the most desirable extraction method in extracting aglycone isoflavones, daidzein and genistein from Korean soybean.

• KSBB Journal
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• v.9 no.4
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• pp.385-394
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• 1994

Large scale purification to get antifungal antibiotic KRF-001 of 90% purity, was investigated using preparative HPLC. Crude KRF-001 was purified by XAD-7 adsorption chromatography, acid precipitation and microfiltration. Microfiltration was the most effective isolation method of crude KRF-001. The purification methods using C18 chromatography was convenient compared with the conventional methods. Delta PAK C18 column and Bonda PAK C18 column were adapted large scale purification of KRF-001. Gradient system of prep HPLC using Delta PAK C18 column was more effective. With these conditions, final recovery of KRF-001 yielded 77%.

• KSBB Journal
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• v.30 no.1
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• pp.1-10
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• 2015

Nanomaterial-based artificial enzymes (Nanozymes) have attracted recent attention because of their unique advantageous characteristics such as excellent robustness and stability, low-cost production by facile scale-up, and longterm preservation capability that are critically required as an alternative to natural enzymes. These nanozymes exhibit natural enzyme-like activity, and they have been applied to diverse kinds of detection methods for disease-associated biomolecules such as DNAs, proteins, cells, and small molecules including glucose. To highlight the progress in the field of disease diagnostics using nanozyme, this review discusses many nanozyme-based detection methods categorized by the types of target biomolecules. Finally, we address the current challenges and perspectives for the widespread utilization of nanozyme-based disease diagnostics.

• Journal of information and communication convergence engineering
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• v.17 no.1
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• pp.49-59
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• 2019

Biosensors, which are analysis devices used to convert biological reactions into electric signals, are made up of a receptor component and a signal transduction part. Graphene quantum dots (GQDs) and carbon quantum dots (CQDs) are new types of carbon nanoparticles that have drawn a significant amount of attention in nanoparticle research. The unique features exhibited by GQDs and CQDs are their excellent fluorescence, biocompatibility, and low cytotoxicity. As a result of these features, carbon nanomaterials have been extensively studied in bioengineering, including biosensing and bioimaging. It is extremely important to find biomaterials that participate in biological processes. Biomaterials have been studied in the development of fluorescence-based detection methods. This review provides an overview of recent advances and new trends in the area of biosensors based on GQDs and CQDs as biosensor platforms for the detection of biomaterials using fluorescence. The sensing methods are classified based on the types of biomaterials, including nucleic acids, vitamins, amino acids, and glucose.