• Title/Summary/Keyword: Biochemical oxygen demand (BOD)

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Change of components in the slaughter waste by Aeromonas hydrophila isolated from slaughter waste (도축폐수에서 분리된 Aeromonas hydrophila에 의한 도축폐수의 성상 변화)

  • 손연주;박재림
    • Journal of Environmental Science International
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    • v.12 no.10
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    • pp.1079-1084
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    • 2003
  • This study was carried out to check changes of components in the slaughter waste by the bacteria isolated from slaughter wastes from Gyungnam Province from May to June 2000, and to find usefu] organism for treatment of the waste. Bacteria used in this study were Aeromonas hydrophila, as the dominant of the waste. Optimum conditions for bacterial culture were obtained as the temperature of 35$^{\circ}C$, pH 6.5, and shaking of 120 rpm in nutrient broth. The mean values of dissolved oxygen was 4.14 mg/1; biochemical oxygen demand, 1731.21 mg/1; ratio of BOD/COD, 0.53-0.64; ratio of T-P/T-N, 1.0-1.41; and viable counts of the waste, 5.47${\times}$10$\^$7/ CFU. Little change in total nitrogen observed by 36 hr of the culture. The largest amount of increasing NH$_4$$\^$+/-N was observed in the sample that 10% of the waste added in nutrient broth with A. hydrophila showing the value of 29.19 mg/l at the beginning to 570.36 mg/1 by 36 hr of culture. However, the highest increasing ratio between initial amount and finals at 36 hr of culture showed as 41.6 times when 3% of the waste added. NO$_3$ -N was decreased showing the value of 71.27 mg/1 to 32.14 mg/1 by 24 hr of culture with the organism when 10% of the waste added in nutrient broth. Total phosphorus was decreased showing the value from 188.74 mg/1 to 101.41 mg/1 after 12 hr of culture with the organism when 5% of the waste added in nutrient broth, while T-P was decreased gradually by 24 hr of culture from 193.8 to 101.4 mg/1 when 10% of the waste added.

Trend Analysis of Water Quality in Dongjin River Watershed (동진강 유역의 수질 경향 분석에 관한 연구)

  • Lee, Hye-Won;Park, Seok-Soon
    • Korean Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.39-47
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    • 2009
  • Spatial and temporal analysis of water quality was performed for eleven monitoring stations in Dongjin River watershed in order to determine the trends of monthly water quality. The monthly water quality data of biochemical oxygen demand (BOD), total nitrogen (TN) and total phosphorus (TP) during $1995{\sim}2004$, were analyzed utilizing Seasonal Mann-Kendall test, LOWESS and three-dimensional graphic approaches. The results indicated that BOD and TN concentrations had the downward trend, but TP showed the upward trend, especially in Gobucheon. This numerical and graphic analysis is the useful tool to analyze the long-term trend of water quality in a large river system.

Temporal and Spatial Evaluation of Water Pollution Characteristics in Gohyeon Stream and Its Tributaries (고현천 및 유입지류의 수질오염 특성의 시·공간적 평가)

  • Kim, Sung-Jae
    • Korean Journal of Environmental Agriculture
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    • v.31 no.3
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    • pp.235-247
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    • 2012
  • BACKGROUND: Gohyeon Stream is the municipal eco-stream of 7.1km in total length which flows through the downtown area of Gohyeon in Geoje city, rising from the watershed of Mundong Water Fall. Gohyeon district in Geoje city has been a rapid growing area centering in Geoje city and then experienced an rapid increase in population. Large amounts of sewage pollutants have been spewed into Gohyeon Stream from its tributaries, due to the lack of sewer system. Gohyeon Stream is laced with unhealthy levels of fecal coliform (FC). Restoration of water quality in Gohyeon Stream is no less inevitable in behalf of its ecosystem and the citizen. In this study, the water quality of Gohyeon Stream and its tributaries was examined temporally and spatially, and their relationships were comparatively analyzed to give useful basic data applying to a restoration project of the water quality of Gohyeon Stream. METHODS AND RESULTS: The samples ware taken at 20 points in Gohyeon Stream and 19 points in its tributaries during the rainy and dry seasons, respectively, and examined on the parameters of pH, temperature, salinity, dissolved oxygen (DO), suspended solid (SS), biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved inorganic nitrogen (DIN; $NH_3$-N, $NO_3$-N, $NO_2$-N), disolved inorganic phosphorus (DIP; $PO_4$-P) and FC. The data were analyzed using a comparative analysis and Pearson's correlation analysis among the parameters. During the rainy season, the concentration of SS was high in the upper region of Gohyeon Stream, and the concentrations of COD, DIN and DIP were low in the upper region and high in the middle and lower regions. During the dry season, the concentration of SS was low and the concentrations of COD, DIN, DIP and FC were high in all regions. The Pearson's correlation analyses showed that the relationships between DO and FC, COD and DIP, and DIN and FC during the rainy season as well as between DO and DIN, SS and FC, COD and DIP, and DIN and DIP during the dry season were significant. CONCLUSION: During the rainy season, the upper region of Gohyeon Stream flowed the high level of SS, the middle region the high level of nutrients due to an agricultural run-off, and the lower region the high level of nutrients due to a sewage inflow. During the dry season, the water quality of Gohyeon Stream was directly and sensitively influenced on the inflow of sewage from the tributaries.

Comparative Assessment of Specific Genes of Bacteria and Enzyme over Water Quality Parameters by Quantitative PCR in Uncontrolled Landfill (정량 PCR을 이용한 비위생 매립지의 특정 세균 및 효소 유전자와 수질인자와의 상관관계 평가)

  • Han, Ji-Sun;Sung, Eun-Hae;Park, Hun-Ju;Kim, Chang-Gyun
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.8
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    • pp.895-903
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    • 2007
  • As for the increasing demanding on the development of direct-ecological landfill monitoring methods, it is needed for critically defining the condition of landfills and their influence on the environment, quantifying the amount of enzymes and bacteria mainly concerned with biochemical reaction in the landfills. This study was thus conducted to understand the fates of contaminants in association with groundwater quality parameters. For the study, groundwater was seasonally sampled from four closed unsanitary landfills(i.e. Cheonan(C), Wonju(W), Nonsan(N), Pyeongtaek(P) sites) in which microbial diversity was simultaneously obtained by 16S rDNA methods. Subsequently, a number of primer sets were prepared for quantifying the specific gene of representative bacteria and the gene of encoding enzymes dominantly found in the landfills. The relationship between water quality parameters and gene quantification were compared based on correlation factors. Correlation between DSR(Sulfate reduction bacteria) gene and BOD(Biochemical Oxygen Demand) was greater than 0.8 while NSR(Nitrification bacteria-Nitrospira sp.) gene and nitrate were related more than 0.9. A stabilization indicator(BOD/COD) and MTOT(Methane Oxidation bacteria), MCR(Methyl coenzyme M reductase), Dde(Dechloromonas denitrificans) genes were correlated over 0.8, but ferric iron and Fli(Ferribacterium limineticm) gene were at the lowest of 0.7. For MTOT, it was at the highest related at 100% over BOD/COD. In addition, anaerobic genes(i.e., nirS-Nitrite reductase, MCR. Dde, DSR) and DO were also related more than 0.8, which showing anaerobic reactions generally dependant upon DO. As demonstrated in the study, molecular biological investigation and water quality parameters are highly co-linked, so that quantitative real-time PCR could be cooperatively used for assessing landfill stabilization in association with the conventional monitoring parameters.

Spatial Variation in Land Use and Topographic Effects on Water Quality at the Geum River Watershed (토지이용과 지형이 수질에 미치는 영향의 공간적 변동성에 관한 연구 - 금강 권역을 중심으로)

  • Park, Se-Rin;Choi, Kwan-Mo;Lee, Sang-Woo
    • Korean Journal of Ecology and Environment
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    • v.52 no.2
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    • pp.94-104
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    • 2019
  • In this study, we investigated the spatial variation in land use and topographic effects on water quality at the Geum river watershed in South Korea, using the ordinary least squares(OLS) and geographically weighted regression (GWR) models. Understanding the complex interactions between land use, slope, elevation, and water quality is essential for water pollution control and watershed management. We monitored four water quality indicators -total phosphorus, total nitrogen, biochemical oxygen demand, and dissolved oxygen levels - across three land use types (urban, agricultural, and forested) and two topographic features (elevation and mean slope). Results from GWR modeling revealed that land use and topography did not affect water quality consistently through space, but instead exhibited substantial spatial non-stationarity. The GWR model performed better than the OLS model as it produced a higher adjusted $R^2$ value. Spatial variation in interactions among variables could be visualized by mapping $R^2$ values from the GWR model at fine spatial resolution. Using the GWR model, we were able to identify local pollution sources, determine habitat status, and recommend appropriate land-use planning policies for watershed management.

The Study on the Amount and Major Compositions of Excreta from Swine (돼지의 분뇨 배설량 및 분뇨 성분 조사)

  • HwangBo, Jong;Hong, Eui-Chul;Park, Hee-Du;Kim, Dong-Woon;Cho, Sung-Back
    • Journal of Animal Science and Technology
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    • v.52 no.4
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    • pp.319-328
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    • 2010
  • This work was carried out to investigate the quantity of excreta and its composition in crossbred pigs (Yorkshire ${\times}$ Landrace ${\times}$ Duroc) at different stages of growth. Twelve young piglets (average BW weight of $19.0{\pm}0.33kg$) were used in this study. Pigs were divided into four phases during growing time and two phases during finishing time. The average excreta production for growing pig was 3.46 kg/head/day (feces: 1.07 kg, urine: 2.39 kg). The average moisture contents of feces and urine were 70.54% and 97.39%, respectively. Contents of Calcium, Magnesium, Copper, Plumbum, and Arsenic were 1.00%, 0.26%, 10.47 mg/kg, 2.43 mg/kg, and 1.02 mg/kg, respectively. The concentration of the water pollutants like Biochemical Oxygen Demand ($BOD_5$), Chemical Oxygen Demand (COD), Suspended Solid (SS), Total Nitrogen (TN) and Total Phosphorus (TP), excreted from pig were 96335, 61073, 207466, 8104 and 4209 mg/L in feces and 7364, 7149, 2715, 10110 and 613 mg/L in urine at the end of test, respectively. The daily loading amount of water pollutants ($BOD_5$, COD, SS, TN, and TP, respectively) in pig excreta were 102.1, 61.8, 221.6, 8.7, and 3.9 g/head/day in feces, and 19.3, 16.7, 8.0, 22.2, and 1.3 g/head/day in urine, respectively. The Nitrogen, $P_2O_5$, and $K_2O$ contents in the excreta of pigs were 0.96, 0.83 and 0.42% in feces, and 0.80, 0.09 and 0.53% in urine, respectively. Finally, this work was suggested to give basic information to swine farms.

A Study on the Filtration of Swine Anaerobic Digestate Using Multi-Layered Compost Beds (다층구조의 퇴비단을 이용한 돈분뇨 슬러리 혐기소화액의 여과효과에 대한 연구)

  • Han, Deug-Woo;Lee, Dong-Hyun;Kim, Jung-Gon;Yang, Seung-Hak;Bae, Jin-Woo;Kwag, Jung-Hoon;Choi, Dong-Yoon;Jeong, Kwang-Hwa
    • Journal of the Korea Organic Resources Recycling Association
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    • v.21 no.4
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    • pp.72-81
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    • 2013
  • The objective of this study was to verify whether SCB(Slurry Composting & Bio-filtration) system can be applied for the treatment of anaerobic digestion(AD) wastewater and also, to identify the most effective set among three filtration compost beds tested. Results can be summarized as these; (a) When AD wastewater was sprayed on the top of beds which were mainly composed of sawdust and/or other media and, subsequently, filtrates collected and analyzed, there were large drop in the values of Electric Conductivity(EC), Total Suspended Solid(TSS), Biochemical Oxygen Demand(BOD), and Chemical Oxygen Demand(COD). In contrast, Total Nitrgen(T-N) and Total Phosphorus(T-P) were progressively elevated. We consider these changes as positive if the filtrate are to be utilized as liquid fertilizer. (b) When three sets of filtration beds (T1, T2, T3) were compared for their effectiveness, no significant difference was found among them. These indicate that expensive sawdust can be replaced in part with cheaper media such as woodchip, rice husks, or others. (c) At early stage of operation (within 20 days), BOD in filtrates were maintained at high level probably due to the lack of microbial activity. During the same stage, T-N, T-P was at low level but, were elevated to higher levels thereafter. These data, when combined, indicate that the filtration system needs at least a couple of weeks for the optimized microbial functioning. (d) The temperatures of the experimental beds were progressively dropped as the experiment continued through the fall season, although filtration effectiveness was not noticeably influenced.

Analysis of Relationship Between Water Quality Parameters in Agricultural Irrigation Reservoirs and Land Uses of Associated Watersheds (농업용저수지 유역의 토지이용과 수질항목 간의 상관관계 분석)

  • Yoon, Chun-Gyeong;Lee, Sae-Bom;Jung, Kwang-Wook;Han, Jung-Yoon
    • Korean Journal of Ecology and Environment
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    • v.40 no.1
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    • pp.31-39
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    • 2007
  • Monitoring data of 48 agricultural irrigation reservoirs from 1999 to 2004 was analyzed for water quality characteristics including biochemical oxygen demand $(BOD_5)$, chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP), and chl-${\alpha}$. Land uses of the watersheds associated with these reservoirs were determined for residential, forest, upland, paddy and miscellaneous, and regressed against water quality characteristics. Correlation analysis showed that forest land use was negatively correlated with all the water quality characteristics implying it's beneficial effects in water quality perspectives. Other land uses including residential, upland, and paddy generally illustrated positive correlation with water quality characteristics, which indicates most human activities of the watershed could degrade water quality of the receiving water bodies. Paddy land use partially contributed to the water quality degradation in contrast to the previous studies. It might be attributed to the relatively clean water quality of the study area, where even slight pollutant loading could degrade sensitively water quality. Further investigation is recommended for the effect of proximity as well as land use portions on the water quality of receiving water body.

Investigation of Microbiological and Physiochemical Quality for Irrigation Water used in Napa Cabbage Cultivation (배추 재배에 이용되는 농업용수의 미생물 오염도 조사 및 이화학성분 분석)

  • Yun, Bohyun;Kim, Min Kyung;Ryu, Jin Hee;Kim, Won-Il;Park, Byeong-Yong;Kim, Hyun-Ju;Lee, Seung-Don;Kim, Se-Ri
    • Journal of Food Hygiene and Safety
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    • v.32 no.5
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    • pp.396-403
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    • 2017
  • The purpose of this study was to investigate water quality for irrigation water used in Napa cabbage cultivation. The water samples were analyzed for physiochemical and microbiological quality for a total of 111 samples including surface water (n = 75) and groundwater (n = 36) collected from five different regions where Napa cabbage is massively grown. As a conclusion, the levels of fecal indicators for surface water were higher than those for groundwater. The numbers of coliform from surface water and groundwater were 1.96-4.96 and 0-3.98 log MPN/100 mL, respectively. Enterococci were detected in 95% (72/75) of surface water samples and 22% (8/36) of groundwater samples. Besides, 97% (73/75) of surface water samples were observed being contaminated with Escherichia coli, and 22% (8/36) of groundwater sample was positive for E. coli. In the case of surface water, E. coli and coliform correlate to T-P, and enterococci showed relevance to the suspended solid (SS) and biochemical oxygen demand (BOD). In groundwater, fecal indicator bacteria showed relevance to the SS and chemical oxygen demand (COD). These results could be provided as fundamental date for establishing microbial standard of water used in leafy vegetables cultivation.

A Study of Antimicrobial Resistance in Escherichia coli and the Distribution of Indicator Microorganisms in Asan City (아산시 지표미생물의 분포와 Escherichia coli의 항생제 내성에 관한 연구)

  • Lee, Geun-Yeol;Kim, Keun-Ha;Kwon, Mun-Ju;Kwon, Hyuk-Ku;Kim, Yeon-Hee;Lee, Jang-Hoon
    • Journal of Environmental Health Sciences
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    • v.36 no.3
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    • pp.229-235
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    • 2010
  • Efforts to evaluate water pollution using indicator microorganisms have been underway for decades, and driven by research on water purity control applications, water quality criteria are growing more and more strict. Furthermore, recent reports indicate that high concentrations of antibiotics are not absorbed, and are present in excrement from animals and humans dosed with unnecessarily high levels of antibiotics. This has emerged as very important issue from the standpoint of being an ecological and health hazard. In this study, water pollution was analyzed through physicochemical and microbiological means, and antibiotic resistance in indicator microorganisms was assessed. In physicochemical analysis, biochemical oxygen demand (BOD)$_5$ and chemical oxygen demand (COD)$_{Mn}$ evaluation showed that pollution by organisms was highest at the G1 location with a high human population, and the DP location which has many livestock-containing households. The indicator organism levels at the G1 location were: Total Coliforms (1205 colony forming units (CFU)/100 ml), Fecal Coliforms (270 CFU/100 ml), Escherichia coli (253 CFU/100 ml) and Fecal Streptococci (210 CFU/100 ml), while for the DP location levels were: Total Coliforms (1480 CFU/100 ml), Fecal Coliforms (438 CFU/100 ml), E. coli (560 CFU/100 ml), and Fecal Streptococci (348 CFU/100 ml). Levels of fecal indicator microorganisms such as Fecal Coliforms, E. coli and Fecal Streptococci were high at all locations in the fall (the period after the rainy season), and the yearly distribution was similar between these organisms. If the number of livestock-containing households was high, almost all strains of E. coli (as distinct from the other indicator organisms) showed resistance to antibiotics, with the degree of resistance varying between areas. E. coli strains from the OY area in particular, which has a high population density, showed strong resistance to AM10 and Va30. While strong antibiotic resistance was observed overall at the DP and OY locations, no resistance was observed at the EB location.