• Environmental Engineering Research
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• 제23권1호
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• pp.99-106
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• 2018

Lack of information on the Biochemical Oxygen Demand (BOD) decay rates of river water under the tropical environment has triggered this study with an aim to fill the gap. Raw sewage, treated sewage, river water and tap water were mixed in different proportions to represent river water receiving varying amounts and types of wastewater and fed in a laboratory flume in batch mode. Water samples were recirculated in the flume for 30 h and BOD and Carbonaceous BOD (CBOD) concentrations were measured at least six times. Decay rates were obtained by fitting the measured data in the first order kinetic equation. After conducting 12 experiments, the range of BOD and CBOD decay rates were found to be 0.191 to 0.92 per day and 0.107 to 0.875 per day, respectively. Median decay rates were 0.344 and 0.258 per day for BOD and CBOD, respectively, which are slightly higher than the reported values in literatures. A relationship between CBOD decay rate and BOD decay rate is proposed as $k_{CBOD}=0.8642_{k_{BOD}}-0.0349$ where, $k_{CBOD}$ is CBOD decay rate and $k_{BOD}$ is BOD decay rate. The equation can be useful to extrapolate either of the decay rates when any of the rates is unknown.

• Journal of Nutrition and Health
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• 제37권7호
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• pp.557-565
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• 2004

This study was conducted to assess the effect of nutrition counseling for postoperative female breast cancer patients (N = 38). In baseline survey, we investigated the nutrition parameters by measuring anthropometric and biochemical blood index and assessing nutrient intake using recall method. Individual nutrition counseling was performed 3 times with 2 - 3 weeks intervals. Patients were offered nutrition information related to breast cancer and desirable life style after cancer operation. The effects of counseling program were assessed 2 month later. Of biochemical parameters, serum total cholesterol level was significantly reduced after nutrition counseling program. Nutrient intakes of postoperative breast cancer patients were generally below the RDA level at the baseline survey. After nutrition counseling, mean daily intake of total energy, protein, calcium were significantly elevated. Of dietary habit, meal regularity and control of portion size were improved and fruits and vegetables intake were significantly increased after nutrition counseling. Control of fatty food intake and alcohol drinking were significantly improved after nutrition counseling program. Score of general nutrition knowledge and knowledge about breast cancer, were significantly increased after nutrition counseling from 2.1 to 3.2 and from 2.3 to 3.8, respectively. It can be postulated that the dietary habit and nutrients intake of postoperative breast cancer patients can be improved by individual nutrition counseling program. In further study, the systematic group nutrition counseling program is needed.

• 한국환경보건학회지
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• 제26권3호
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• pp.11-17
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• 2000

There are among the most relevant toxic emissions from incinerators such as polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), dioxin-like polychlorinated biphenyls (PCBs). Induction of cytochrome P4501A1 catalyzed 7-ethoxyresorufin O-deethylase(EROD) activity in mammalian cell culture(EROD bioassay) is thought to be a selective and sensitive parameter used for the quantification of dioxin-like components. In this study, the toxic emissions from several school waste incinerators were evaluated by determination of CYPIA catalytic activity and cytotoxicity using cell culture microbioassay. The incinerator residue and soil samples were collected from the schools located in Kyunggi province from April to June 1999. The samples were extracted in a Soxhlet apparatus using toluene for 20 hours. In order to clean-up, concentrated crude extracts were applied to basic alumina column. The EROD activities of extracts in the H4IIE cells were from 1.91$\pm$0.32 ng-TEQ/g to 24.54$\pm$3.48 ng-TEQ/g of biochemical-TEQ value. In soil samples, CYP1A catalytic activity was 0.09~0.64 ng-TEQ/g. EROD bioassay, seems to be a useful short-term bioassay when information about the biological response of complex environmental samples is needed. Although further study is needed, these results indicate that the potent toxic emissions are produced from school waste semi-incinerators.

• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.571-578
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• 2018

Biofuel production using lignocellulosic biomass is gaining attention because it can be substituted for fossil fuels without competing with edible resources. However, because Saccharomyces cerevisiae does not have a ${\text\tiny{D}}$-xylose metabolic pathway, oxidoreductase or isomerase pathways must be introduced to utilize ${\text\tiny{D}}$-xylose from lignocellulosic biomass in S. cerevisiae. To elucidate the biochemical properties of xylose isomerase (XI) from Piromyces sp. E2 (PsXI), we determine its crystal structure in complex with substrate mimic glycerol. An amino-acid sequence comparison with other reported XIs and relative activity measurements using five kinds of divalent metal ions confirmed that PsXI belongs to class II XIs. Moreover kinetic analysis of PsXI was also performed using $Mn^{2+}$, the preferred divalent metal ion for PsXI. In addition, the substrate-binding mode of PsXI could be predicted with the substrate mimic glycerol bound to the active site. These studies may provide structural information to enhance ${\text\tiny{D}}$-xylose utilization for biofuel production.

• Journal of the Optical Society of Korea
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• 제18권6호
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• pp.691-697
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• 2014

Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

• KSBB Journal
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• 제21권4호
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• pp.231-235
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• 2006

유전자 생물학 분야에서 적용가능 한 세포간 네트워크를 입증하는 고처리 정보공학에 응용하려는 수치학적인 표현 모델을 분석 연구한다. 확률적 그래프 모델을 사용하여 데이터 네트워크로부터 생물학적 통찰력을 확률적 함수적으로 응용해 복잡한 세포간 네트워크보다 단순한 하부모델로 구성하여 유전자 베이스네트워크 논리를 유전자 표현 레벨로 나타낸다. 유전자 데이터로부터 확률적 그래프 모델들을 분석하여 유전자 표현 데이터를 정보공학 네트워크 모델의 방법으로 확장 추론한다.

• 한국작물학회지
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• 제41권5호
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• pp.563-568
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• 1996

담배 생육단계별 RNA, protease 활성도와 단백질 패턴의 변화를 파악하여 노화가 진행되는 과정에서 생리 ㆍ생화학적인 변화의 기초자료를 얻고자 본 실험을 수행하였으며 결과는 다음과 같다. 가용성 단백질 함량은 출엽 후 15일까지 증가하여 출엽 후 35일까지 일정하게 유지하였다. 총 RNA 함량은 출엽 후 15일에 가장 높았으며 출엽후 30일까지 급격한 감소를 보였다. Protease 활성 변화는 중성 protease (pH 7.8)가 활성이 높았으며 노화말기인 출엽 후 50일부터 갑자기 증가하였다. 전기영동 패턴은 큰 변화가 없었으나 61.0kd의 polypeptide은 출엽 후 35일부터 생성되어 노화말기까지 증가하였다.

• 동아시아식생활학회지
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• 제24권6호
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• pp.691-699
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• 2014

The purpose of this study was to examine the effect of temple food intake on health improvement. The survey was conducted from September to October 2013. The subjects in this study were 46 adults who participated in short-term 'Temple Stay' program. Every meal was prepared according to prearranged temple food menu, and it was provided to subjects for 4 weeks. Dietary intake, anthropometric measurements and biochemical parameter were measured and nutritional & health status were analyzed to find the effect of the program. Nutrients content of the temple food was sufficient to dietary reference intake while content of fat, fiber, vitamin A and calcium from the meal was significantly higher than the subjects' usual diet. Mean body weight, BMI and blood pressure (systolic and diastolic) related with obesity and hypertension decreased after the program. The index referring to liver function and cardiovascular disease risk such as serum ALT, ${\gamma}$-GTP, TG and LDL decreased while HDL increased thereby showing positive effect. The above results anticipate that temple foods with an affirmative effect in improving health status have a potential benefit that can be applied to general food service. Further detailed study might provide information in developing healthy diet for a corresponding clinical problem.

• Mycobiology
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• 제36권1호
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• pp.74-76
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• 2008

To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, $\beta$-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.

• 대한수의학회지
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• 제57권1호
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• pp.43-45
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• 2017

This study investigated changes in certain blood parameters in calves and pigs after foot-and-mouth disease (FMD) vaccination. In this study, five calves and five pigs were selected from groups of 10 calves and pigs, respectively, and were vaccinated with an FMD vaccine. The remaining animals formed two non-treatment control groups. Blood samples were collected from all animals on the 1st, 3rd, 5th, and 7th days post-vaccination. In the FMD-vaccinated calves and pigs on day 7 post-vaccination, white blood cell counts, blood urea nitrogen levels, and alanine aminotransferase and aspartate aminotransferase activities were higher than those in the respective controls. The present data suggested that the certain hemato-biochemical parameters on cattle and pigs were meaningfully changed between before and after FMD vaccination.