• Title/Summary/Keyword: Biochemical characteristics

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Comparison of Biochemical Characteristics of Myofibrillar Proteins from Red Muscle and White Muscle (Red muscle과 White muscle의 근원섬유단백질의 특성의 비교)

  • Yang, Ryung;Shin, Wan-Chul;Oh, Doo-Whan;Jhin, Hong-Seung;Kim, Kee-Tae
    • Korean Journal of Food Science and Technology
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    • v.18 no.3
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    • pp.173-180
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    • 1986
  • To investigate on the biochemical characteristics of muscle fiber, myofibrils and actomyosins were prepared front red muscle and white muscle, and their ATPase activities and SDS-polyacrylamide gel electrophoretic patterns were compared. Also biochemical characteristics of bovine muscle were compared with those of chicken muscle for the detection of species characteristics. SDS-polyacrylamide gel electrophoretic analysis indicated that red muscle contained nlore 30K component of myofibril than white muscle. Differences in KCI concen-tration dependency of actomyosin ATPase activities and ATPase activity-pH cone were observed, when bovine muscle were compared with chicken muscle.

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Photocatalytic Properties of TiO2 According to Manufacturing Method (제조방법에 따른 TiO2의 광촉매 특성 분석)

  • Lee, Hong Joo;Park, Yu Gang;Lee, Seung Hwan;Park, Jung Hoon
    • Korean Chemical Engineering Research
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    • v.56 no.2
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    • pp.156-161
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    • 2018
  • $TiO_2$ photocatalyst powders were prepared by chlorination method and sol-gel method. Specific surface area and crystalline (i.e., anatase and rutile) of the catalyst varied depending on manufacture conditions and method. TTIP-sol photocatalyst had higher methylene blue (MB) decomposition characteristics than photocatalyst from chlorination method and TBOT-sol. MB removal efficiency from aqueous solution with TTIP-sol photocatalyst was over 90%. Experimental results showed that the $TiO_2$ photocatalyst with a single anatase phase and a large specific surface area had high decomposition characteristics of organic materials.

Strain Improvement of Candida tropicalis for the Production of Xylitol: Biochemical and Physiological Characterization of Wild-type and Mutant Strain CT-OMV5

  • Rao Ravella Sreenivas;Jyothi Cherukuri Pavanna;Prakasham Reddy Shetty;Rao Chaganti Subba;Sarma Ponnupalli Nageshwara;Rao Linga Venkateswar
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.113-120
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    • 2006
  • Candida tropicalis was treated with ultraviolet (UV) rays, and the mutants obtained were screened for xylitol production. One of the mutants, the UV1 produced 0.81g of xylitol per gram of xylose. This was further mutated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), and the mutants obtained were screened for xylitol production. One of the mutants (CT-OMV5) produced 0.85g/g of xylitol from xylose. Xylitol production improved to 0.87 g/g of xylose with this strain when the production medium was supplemented with urea. The CT-OMV5 mutant strain differs by 12 tests when compared to the wild-type Candida tropicalis strain. The XR activity was higher in mutant CT-OMV5. The distinct difference between the mutant and wild-type strain is the presence of numerous chlamvdospores in the mutant. In this investigation, we have demonstrated that mutagenesis was successful in generating a superior xylitol-producing strain, CT-OMV5, and uncovered distinctive biochemical and physiological characteristics of the wild-type and mutant strain, CT-OMV5.

Comparison of conservative therapy and steroid therapy for Bell's palsy in children

  • Yoo, Hye Won;Yoon, Lira;Kim, Hye Young;Kwak, Min Jung;Park, Kyung Hee;Bae, Mi Hye;Lee, Yunjin;Nam, Sang Ook;Kim, Young Mi
    • Clinical and Experimental Pediatrics
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    • v.61 no.10
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    • pp.332-337
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    • 2018
  • Purpose: Bell's palsy is characterized by sudden onset of unilateral facial weakness. The use of corticosteroids for childhood Bell's palsy is controversial. This study aimed to identify clinical characteristics, etiology, and laboratory findings in childhood Bell's palsy, and to evaluate the efficacy of corticosteroid treatment. Methods: We conducted a retrospective analysis of children under 19 years of age treated for Bell's palsy between January 2009 and June 2017, and followed up for over 1 month. Clinical characteristics, neuroimaging data, laboratory findings, treatments, and outcomes were reviewed. Patients with Bell's palsy were divided into groups with (group 1) and without (group 2) corticosteroid treatment. Differences in onset age, sex, laterality, infection and vaccination history, degree of facial nerve palsy, and prognosis after treatment between the groups were analyzed. Results: One hundred patients were included. Mean age at presentation was $7.4{\pm}5.62years$. A total of 73 patients (73%) received corticosteroids with or without intravenous antiviral agents, and 27 (27%) received only supportive treatment. There was no significant difference in the severity, laboratory findings, or neuroimaging findings between the groups. Significant improvement was observed in 68 (93.2%) and 26 patients (96.3%) in groups 1 and 2, respectively; this rate was not significantly different between the groups (P=0.48). Conclusion: Childhood Bell's palsy showed good prognosis with or without corticosteroid treatment; there was no difference in prognosis between treated and untreated groups. Steroid therapy in childhood Bell's palsy may not significantly improve outcomes.

General Characteristics, Antherpometric Values and Health-Related Factors according to Sasang Constitution among Elderly People in Rural Areas (농촌지역 50세 이상 성인 및 노인의 사상체질에 따른 일반적 특성, 신체계측 및 건강관련인자에 관한 연구)

  • Yang, Kyung-Mi
    • Journal of the Korean Home Economics Association
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    • v.50 no.2
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    • pp.39-51
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    • 2012
  • The purpose of this study was to elucidate the general characteristics, antheropometric values and health-related characteristics of the rural elderly population according to their Sasang constitutions. The participants in this study were 31 in Taeeumin group, 29 in Soyangin group, and 15 in Soeumin group. The present study was performed using self-administration questionnaires, antheropometric and biochemical analysis. There were no significant differences in height, weight, BMI, diastolic blood pressure and alcohol habits between the Sasang constitutions. In the analysis of physical activity, Soyangin had a higher value than the other groups. Compared with the other groups, Soeumin also had considerable resistance to stress($101.1{\pm}26.2$ point). Furthermore, in the analysis of fatigability caused by stress, Soeumin was mostly shown to feel less fatigue than the other groups. On the other hand, Taeeumin appeared to feel more fatigue. In the biochemical analysis of blood, there was no significant difference between Sasang constitutions.

Morphological, Physiological and Biochemical Responses of Gerbera Cultivars to Heat Stress

  • Chen, Wen;Zhu, Xiaoyun;Han, Weiqing;Wu, Zheng;Lai, Qixian
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.1-14
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    • 2016
  • Heat stress is an agricultural problem for Gerbera jamesonii, and it often causes poor seedling growth, reduced flower yield and undesirable ornamental characteristics of flowers. However, little is known about the effect of heat stress on the morphological, physiological and biochemical characteristics of gerbera plants. Here, the responses of six cultivars of Gerbera jamesonii to heat stress were investigated. Under a 1-d heat treatment at $45^{\circ}C$, the leaves of gerbera cultivars showed yellowing, wilting, drying and death to varying degrees. The heat treatment also resulted in increased electrical conductivity, decreased soluble protein and chlorophyll contents, and the accumulation of malondialdehyde (MDA) and proline in leaves. Moreover, heat tolerance differed among the six tested gerbera cultivars. Our results demonstrated that among the six gerbera cultivars, 'Meihongheixin' is a heat-resistant cultivar, whereas 'Beijixing' is a heat-sensitive one. 'Shijihong' and 'Linglong' are relatively heat-resistant cultivars, and 'Dadifen' and 'Taiyangfengbao' are relatively heat sensitive.

Studies on the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus (양식넙치에서 분리된 Edwardsiella tarda의 생화학적 및 혈청학적 특성에 관한 연구)

  • Bang, Jong-Deuk;Chun, Seh-Kyu;Park, Soo-Il;Choi, Yun-Jung
    • Journal of fish pathology
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    • v.5 no.1
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    • pp.29-35
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    • 1992
  • This study was carried out in order to identify the biochemical and serological characteristics of Edwardsiella tarda isolated from cultured flounder, Paralichthys olivaceus in the east and south coast of Korea. During the year of 1990 and 1991, the number of isolated E. tarda were 131 strains. To identify the biochemical characteristics of them kinds of tests were conducted. The results represented that all the strains had the same biochemical characteristics, and their biochemical characteristics were no differences among strains. A serological analysis was carried out based on agglutination test with antiserum belonging to E. tarda serotype a (E-22), b (SU-138), c (SU-100) classfied in japan. The selected 10 isolates showed agglutinin titer of 5120-2560, 40-80 and below 40 against E. tarda serotype a, b and c, respectively. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) profiles of cell proteins of selected 10 isolates were showed no differences in kinds and volumes of proteins among strains.

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Antimicrobial Susceptibility and Biochemical Characteristics of Streptococcus suis Isolated from Diseased Pigs in Gyeongbuk Province (경북지방 환돈에서 분리한 Streptococcus suis의 생화학적 성상 및 약제감수성)

  • Choi, Seong-Kyoon;Kim, Seong-Guk;Kim, Young-Hoan;Choi, Jeong-Hye;Jo, Min-Hee;Cho, Gil-Jae
    • Journal of Life Science
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    • v.20 no.10
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    • pp.1549-1555
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    • 2010
  • Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp from all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.

Anthropometric, Biochemical Characteristics, Nutrient Intakes and Bone Density by Smoking Period in Elderly Male Smokers: Analysis of Data from Korea National Health and Nutrition Examination Survey (KNHANES), 2008~2011 (흡연 노인 남성의 흡연기간에 따른 신체적, 생화학적 특성, 영양소섭취 및 골밀도 차이 : 2008~2011년 국민건강영양조사 자료 분석)

  • Choi, Soon-Nam;Jho, Kwang-Hyun;Chung, Nam-Yong
    • Journal of the Korean Dietetic Association
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    • v.21 no.3
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    • pp.181-193
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    • 2015
  • This study investigated anthropometric and biochemical characteristics, nutrient intakes, and bone density of Korean elderly men (over 65 yrs). Data on bone density and anthropometric (height, weight, waist circumference, body mass index and blood pressure) and biochemical (total cholesterol, high-density lipoprotein (HDL)-cholesterol, low-density lipoprotein (LDL)-cholesterol, triglyceride and hemoglobin) characteristics, nutrient intakes, and nutrient density were obtained from the Fifth Korea National Health and Nutrition Examination Survey (KNHANES, 2008~2011). Subjects were categorized into smoking and non-smoking groups, and smoking groups were divided into three groups by smoking period (under 20 yrs, 21~40 yrs and over 41 yrs). Serum triglyceride concentrations of the smoking group aged 21~40 yrs were higher than those of other groups (P<0.01), whereas other biochemical factors were not different. Intake ratios of energy, protein, phosphorus and sodium in subjects were over 100% of Dietary Reference Intake for Koreans (KDRI). Nutrient densities according to intakes of thiamin, riboflavin, and niacin per 1,000 kcal were significantly different among the groups (P<0.05). Bone density of subjects decreased according to smoking period (T-score of total femur in non-smoking group -0.3108, and -0.2918, -0.4941, -0.6847 in smoking group, respectively). Ratio of osteoporosis was 38.1% in the non-smoking group and 44.4%, 51.1%, and 64.0% in the smoking group, respectively. The findings of the present study show that smoking may be associated with bone health, higher ratio of osteoporosis, and low nutrient density in elderly men. Therefore, practical and systematic non-smoking programs are required to improve the bone density of elderly men as well as maintain healthy bone levels and desirable lifestyle.

Study on Genipin: A New Alternative Natural Crosslinking Agent for Fixing Heterograft Tissue

  • Yoo, Jae-Suk;Kim, Yong-Jin;Kim, Soo-Hwan;Choi, Seung-Hwa
    • Journal of Chest Surgery
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    • v.44 no.3
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    • pp.197-207
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    • 2011
  • Background: In cardiac surgery, especially in the reconstruction of vascular structures and intracardiac defects, glutaraldehyde has usually been used as the reagent for fixing porcine or bovine pericardial tissues. But the well-known problem of calcification or cytotoxicity of glutaraldehyde motivates the search for a replacement. The aim of this study is to investigate the physical, mechanical, and biochemical characteristics of bovine pericardial tissues fixed with genipin, which is known to be a less toxic and more natural fixing reagent. Materials and Methods: Bovine pericardial tissues were fixed with different concentrations and conditions of glutaraldehyde and genipin. To determine the physical, mechanical, and biochemical differences among different concentrations and conditions, we divided the tissue into 18 groups by concentration, the addition of organic solvents, and the timing of adding the organic solvents, and compared the characteristics of each group. Results: Tensile strength, physical activity, and thermal stability tests revealed that the tissues fixed with glutaraldehyde were better with regard to mechanical strength and biochemical durability. However, the difference was not significant statistically. Conclusion: Genipin can be used as an alternative crosslinking agent for pericardial tissue, considering given its physical, mechanical, biochemical characteristics and low cytotoxicity comparable to glutaraldehyde. However, further studies are needed on the immune reaction and the long term changes in genipin-fixed tissues in the human body.