• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.146-147
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• 2003

The free-living nematode, Caenorhabditis elegans (C. elegans) has been adopted as a multicellular biosensor of biological toxicity for alkylphenol, organotin compounds and heavy metals. To adopt as a biosensor, suitability to assess must be fulfilledthrough several criteria; the organism must be sensitive to the testing toxicants, easy to manage in the laboratory and available throughout the year. C. elegans widely used as a simple multicellular organism in developmental biology studies and satisfies all these criteria, and its culture conditions, developmental staging, anatomy and genetic properties are well defined. In addition, researchers can take advantage of the worm's short life cycle, low cost and little individual variation. Moreover, genomic sequencing of C. elegans has recently been completed. With these aspectsof the organism, C. elegans become a more potent model organism for basic and applied bioassays.

• 환경위생공학
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• 제15권2호
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• pp.1-9
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• 2000

This research employed a senescence-accelerated mouse(SAM) to explore the possibility that differences exits among the major antioxidants, superoxid dismutase(SOD), in terms of ability to protect such animal treated with Cu, Fe and Mn. To assess the antioxidants function of metal ions on SAM-R/1 and SAM-P/8 were administered with Cu, Fe and Mn orally. The effect of metal ions on SAM towards reversing oxygen sensitivity was determined as a bioassays of SOD in the mouse liver. The data show that the SOD activity was induced by each metal ions in both SAM-R/1 and SAM-P/8. It suggested that induced SOD by each metal ions may protect against oxidative mediated stress. Finally, overall data lead to the possibility of metal ions as an antioxidants or each metal ions act producer of oxygen radicals in the liver of SAM-R/1 and SAM-P/8.

• 환경위생공학
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• 제14권2호
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• pp.8-17
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• 1999

This research employed a senescence-accelerated mouse(SAM) to explore the possibility that differences exist among the major antioxidatns, lipid peroxidation in terms of ability to protect such animal treatment PQ, SAM-R/1 and SAM-P/8 were administered with PQ(200ppm/Kg) orally. The toxicity of PQ on SAM was determined as a bioassays of SOD, catalase and lipid peroxidation in the mouse liver. The data show that the SOD activity was induced by paraqwuat terement in both SAM-R/1 and SAM-P/8. The degree of lipid peroxidation was increased with PQ treatment. This means that SOD rather than catalase may protect against oxygen radical toxicity. Finally, over data lead to the toxicity of PQ and its function may efect to the antioxidants including SOD, catalase and lipid peroxidation in both SAM-R/1 and SAM-P/8 .

• Fibers and Polymers
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• 제7권1호
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• pp.30-35
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• 2006

The influences of a series of new azo direct dyes including copper-complexes based on benzidine congeners, 2,2'-dimethyl-5,5'-dipropoxybenzidine and 5,5'-dipropoxybenzidine, were examined using microorganism, Daphnia magna. The purpose of the research described in this paper was to use bioassays with daphnids to determine the aquatic toxicity of new azo dyes in which copper was incorporated. The results clearly show that copper has negative effects to aquatic ecosystem as expected. The study also suggested that the assay with Daphnia magna was an excellent method to evaluate the influences of dyes to the aquatic environment.

• The Korean Journal of Ecology
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• 제11권2호
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• pp.65-76
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• 1988

A number of laboratory and field experiments have performed with aqueous extracts from fresh leaves, fallen leaves and roots of Pinus rigida in order to investigae the effetct of the pine tree. The results obtained found its inhibitive effect on the germination and growth of tested species. Though relative germination ratio of the species was somewhat lower than the control plot, severe inhibition was observed in relative dry weight ration, These results were more obvious in aqueous extracts than in the case of leachates and similar results were also confirmed by field bioassays on the forest soil of P. rigida. Therefore, to find out the inhibitory sybstancesm 14 different kinds of KDICical substances were isolatd from the leaved of the tree by chromato-graphy and nist if them were confirmed to be phenoic compounds, The growth of lettuce was inhibition was observed in the concentration of less than 5X10-3M soluton. Phenolic compounds are assumed the substance related to the allelopathic effect of P. rigida.

• Asian-Australasian Journal of Animal Sciences
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• 제13권2호
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• pp.188-191
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• 2000

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2467-2472
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• 2010

Twenty novel tetramic acid derivatives (E)-3-(1-(alkyloxyamino)ethylidene)-1-alkylpyrrolidine-2,4-diones were synthesized by the reaction of 3-(1-hydroxyethylidene)pyrrolidine-2,4-diones with O-alkyl hydroxylamines. The title compounds were confirmed by IR, $^1H$ NMR, MS and elemental analysis. The structure of compound 6r was further verified by X-ray diffraction crystallography. The bioassays showed that most of the title compounds exhibited noticeable herbicidal and fungicidal activities.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.622-629
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• 2010

Biosynthetic studies on brasiliamides, potently convulsive and bacteriostatic compounds from an endophytic Penicillium brasilianum isolated from Melia azedarach (Meliaceae), confirms their phenylpropanoid origin, which is very uncommon in fungi. Feeding experiments with [$2-^{13}C$]-phenylalanine indicated the incorporation of two units of this amino acid on brasiliamide structures. The first step in the phenylpropanoid pathway to those compounds was evaluated through enzymatic bioassays and confirmed the phenylalanine ammonia-lyase (PAL) participation. The metabolism of phenylalanine in this fungus is discussed.

• Natural Product Sciences
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• 제9권3호
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• pp.174-176
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• 2003

Studies on the stem bark extracts of Garcinia cuneifolia have furnished a new xanthone cuneifolin (1) and the triterpene stigmasterol (2). Structures for these compounds were elucidated based on NMR, 2D NMR, MS and GCMS data. Larvicidal activity screening of the crude bark extract using the larvae of Aedes aegypti indicated the larvae to be susceptible to these extracts. $LC_{50}$ values of the bioassays show the extracts to be moderately toxic to the larvae of Aedes aegypti.

• 생약학회지
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• 제20권2호
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• pp.117-122
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• 1989

The effect of wild vegetables such as Allium tuberosum, Allium monanthum, Sedum sarmentosum, Ixeris dentata and Capsella-bursa pastoris on hexobarbital induced hypnosis was tested in mice. Among them, the methanol extract of Allium tuberosum exhibited significant lengthening of the barbiturate hypnosis. When various fractions prepared from the methanol extract of the Allium tuberosum were administered, the chloroform, ethylacetate and butanol extracts caused a significant activity. Through systematic fractionation by $SiO_2$ column monitoring by bioassays, $1,2,3,4-tetrahydro-{\beta}-carboline$ 3-carboxylic acid from the butanol extract was isolated as one of the active principles of this plant.