• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.853-858
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• 2003

Helicobacter pylori specifically adhere to host cells through a number of putative receptors and ligands, mainly based on carbohydrate-protein interactions. Polysaccharide fractions isolated from the leaves of Artemisia capillaris showed different inhibitory activities against H. pylori adhesion by using hemagglutination assay. Among these fractions, an acidic polysaccharide fraction FlA showed highly effective inhibitory activity, and its minimum inhibition concentration was 0.63 mg/ml. The inhibition results by the hemagglutination assay were consistent with those obtained by the enzymelinked glycosorbent assay, which was developed by the conjugation of horseradish peroxidase with fetuin, a sialic acid-containing glycoprotein which was specific to H. pylori adhesion. FlA contained the highest carbohydrate content among polysaccharide fractions, and no protein was detectable when further purified by gel filtration FPLC. Sugar composition analysis using GC revealed the highest amount of galacturonic acid among sugars, which suggests that FlA contains essentially acidic polysaccharides. Our data suggest that acidic polysaccharides may play an important role in the inhibition of H. pylori adhesion to host cells.

• Journal of Microbiology and Biotechnology
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• v.23 no.11
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• pp.1554-1559
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• 2013

A protease produced by Bacillus polyfermenticus SCD was purified and characterized as a new detergent material. The protease was purified from supernatant produced by B. polyfermenticus SCD, by ammonium sulfate precipitation, ion-exchange chromatography on a DEAE-Sephadex A-50, and finally gel filtration chromatography on Sephadex G-50. The molecular mass of this enzyme was 44 kDa based on SDS-PAGE. The optimum temperature and pH were $50^{\circ}C$ and pH 8.0. The ranges of its stability to the pH and temperature were 7.0 to 9.0 and under $40^{\circ}C$, respectively. The enzyme was highly stable in the presence of the surfactants like Triton X-100 (0.1%), showing a 2-fold increase in its proteolytic activity. However, the enzyme was slightly inhibited by the chelating agent EDTA (1 mM). The enzyme has a maximum activity at $50^{\circ}C$ and the activity can be increased by surfactants such as Triton X-100 and Tween 80.

• Proceedings of the Korea Information Processing Society Conference
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• 2007.05a
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• pp.285-288
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• 2007

There is substantial evidence from earlier researches that older adults have difficult seeing under low illumination and at night, even in the absence of ocular diseases. During human aging, there is a rampant decrease in rod/cone-meditated adaptation which is caused by delayed rhodopsin regeneration and pigment depletion. This calls for a need to develop appropriate visual gadgets to effectively aid the aging generation. Our research culminates its approach from Pattanaik's model by making extensions to temporal visual filtering, thereby simulating a reduction of visual response which comes with age. Our filtering model paves way and lays a foundation for future research to develop a more effective adaptation model that may be further used in developing visual content adaptation aids and guidelines in MPEG 21 environment. We demonstrate our visual model using a High Dynamic Range image and the experiment results are in conversant with the psychophysical data from previous vision researches.

• Herbal Formula Science
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• v.20 no.2
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• pp.47-54
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• 2012

Objectives : This study aimed to build up information system for the 25 cases of Korean Medicine (KM) prescriptions which are used mostly often in clinic and aimed to offer information about its effect and safety to public and researcher. Methods : We used KM prescription data-bank which came from 'The Bio-informatics for herbal formula' and 'The evidence based medicine for herbal formula' projects of Korean Institute of Oriental Medicine (KIOM) Results : First, we analyzed prescription data and categorized 7 classes, then we built up prescription database based on it. Second, we designed and developed user functions of prescription information system, then we link it to OASIS. Third, we developed adminstration system to manage the prescription data. Conclusions : From this study we hope that KM prescription information system will be used for fusion research based on KM. Because the implemented system is linked to OASIS, we expect it will be widely used.

• Korean Journal of Oriental Medicine
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• v.14 no.2
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• pp.121-129
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• 2008

The fusion research subjects of Oriental medicine and Information-Technology are actively advanced. These researches provide Oriental medicine the objectivity and support the infra to all study area of Oriental medicine. This paper considers the inside and outside of the country technical development trend of ontology research by analyzing papers and going through case study. It executed information analysis about changes to number of research papers, present state and star higher officer of research facility from the dissertation which it sees. It is known that our country research result is slight so far in quantity and quality as result of analysis. But hereafter it contains many developmental possibilities. Also it reflects the appearance and a growth of new field like bio-informatics biology. In the area of medicine, ontology used to define the terminology for information documentation and the medical terms linked up by high correlation. Also medical information system developed briskly using ontology technology. The ontology of traditional korean medicine play an important role in base infra of traditional korean medicine EHR(Electronic health record).

• Proceedings of the Korea Information Processing Society Conference
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• 2005.05a
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• pp.31-34
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• 2005

최근 국, 내외에서 생물정보학 데이터베이스 구축이 활발히 진행되어 왔고, 각 바이오 정보 시스템의 데이터 통합 연구가 진행 중이다. 대표적인 바이오 데이터베이스 시스템인 GenBank, DDBJ, EBML 등은 같은 의미의 데이터라 하더라도 각 시스템의 내부 데이터 구조 및 데이터 표현 형식이 상이하여 통합에 어려움이 따른다. 이를 해결하기 위해 통합 데이터 형식을 지원하는 웹 서비스 기반 데이터 통합 방식이 제안되고 있다. 현재 국내 웹 서비스 기반의 바이오 정보 제공 사이트들은 SOAP을 이용한 단순 메시지 전달 기법으로 초보적인 단계라 할 수 있다. 본 논문에서는 SOAP을 이용한 단순한 메시지 전달 기법만이 아닌 레지스트리 서버 검색을 통해 서비스 제공자를 찾고, WSDL문서를 분석한 후 사용자에게 검색 메소드를 제공함으로써 빠르고 정확한 서비스를 제공하여 기존에 구축된 시스템의 단점을 보완한다. 또한 상이한 스키마로 이루어진 데이터들을 효과적으로 통합하기 위해 온톨로지를 이용한 웹 서비스 기반 바이오 정보 시스템을 제안하고 구현한다.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.138-144
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• 2008

The production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin (BEA) was studied in submerged cultures of Fusarium oxysporum KFCC 11363P isolated in Korea. The influences of various factors on mycelia growth and BEA production were examined in both complete and chemically defined culture media. The mycelia growth and BEA production were highest in Fusarium defined medium. The optimal carbon and nitrogen sources for maximizing BEA production were glucose and $NaNO_3$, respectively. The carbon/nitrogen ratio for maximal production of BEA was investigated using response surface methodology (RSM). Equations derived by differentiation of the RSM model revealed that the production of BEA was maximal when using 108 mM glucose and 25 mM $NaNO_3$.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.85-86
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• 2001

Indole-3-carbinol (I3C), one component of cruciferous vegetables (the Family of Cruciferae), has been shown to exert chemopreventive effects in liver, colon and mammary tissue, but there has been substantial evidence that consumption of I3C induces tumor promotion in some tissues. Our studies were investigated to examine the modifying effects of I3C in the 7, 12-dimethylbenz［a］anthracene (DMBA) induced rat mammary tumor model.(omitted)

• Journal of Ginseng Research
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• v.38 no.1
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• pp.47-51
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• 2014

The transformation of ginsenoside Rb1 into a specific minor ginsenoside using Aspergillus niger KCCM 11239, as well as the identification of the transformed products and the pathway via thin layer chromatography and high performance liquid chromatography were evaluated to develop a new biologically active material. The conversion of ginsenoside Rb1 generated Rd, Rg3, Rh2, and compound K although the reaction rates were low due to the low concentration. In enzymatic conversion, all of the ginsenoside Rb1 was converted to ginsenoside Rd and ginsenoside Rg3 after 24 h of incubation. The crude enzyme (b-glucosidase) from A. niger KCCM 11239 hydrolyzed the ${\beta}$-($1{\rightarrow}6$)-glucosidic linkage at the C-20 of ginsenoside Rb1 to generate ginsenoside Rd and ginsenoside Rg3. Our experimental demonstration showing that A. niger KCCM 11239 produces the ginsenoside-hydrolyzing b-glucosidase reflects the feasibility of developing a specific bioconversion process to obtain active minor ginsenosides.

• The KIPS Transactions:PartA
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• v.17A no.5
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• pp.221-228
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• 2010

Since string operations were applied to computational biology, security and search for Internet, various data structures and algorithms for computing efficient string operations have been studied. The all-pairs suffix-prefix matching is to find the longest suffix and prefix among given strings. The matching algorithm is importantly used for fast approximation algorithm to find the shortest superstring, as well as for bio-informatics and data compressions. In this paper, we propose an algorithm to find all-pairs suffix-prefix matching using the suffix array, which takes O($k{\cdot}m$)�� time complexity. The suffix array algorithm is proven to be better than the suffix tree algorithm by showing it takes less time and memory through experiments.