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http://dx.doi.org/10.4014/jmb.1306.06073

Purification and Characterization of Heat-Tolerant Protease Produced by Bacillus polyfermenticus SCD  

Choi, Gooi Hun (Division of Animal Life Science, Konkuk University)
Jo, Mi Na (Division of Animal Life Science, Konkuk University)
Kim, Jin-Man (Lotte R&D Center)
Kim, Cheon-Jei (Division of Animal Life Science, Konkuk University)
Kim, Kee-Tae (Bio/Molecular Informatics Center, Konkuk University)
Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.11, 2013 , pp. 1554-1559 More about this Journal
Abstract
A protease produced by Bacillus polyfermenticus SCD was purified and characterized as a new detergent material. The protease was purified from supernatant produced by B. polyfermenticus SCD, by ammonium sulfate precipitation, ion-exchange chromatography on a DEAE-Sephadex A-50, and finally gel filtration chromatography on Sephadex G-50. The molecular mass of this enzyme was 44 kDa based on SDS-PAGE. The optimum temperature and pH were $50^{\circ}C$ and pH 8.0. The ranges of its stability to the pH and temperature were 7.0 to 9.0 and under $40^{\circ}C$, respectively. The enzyme was highly stable in the presence of the surfactants like Triton X-100 (0.1%), showing a 2-fold increase in its proteolytic activity. However, the enzyme was slightly inhibited by the chelating agent EDTA (1 mM). The enzyme has a maximum activity at $50^{\circ}C$ and the activity can be increased by surfactants such as Triton X-100 and Tween 80.
Keywords
Bacillus polyfermenticus SCD; protease; purification; surfactant; Triton X-100; Tween 80;
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