• Restorative Dentistry and Endodontics
• /
• v.41 no.1
• /
• pp.29-36
• /
• 2016

Objectives: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.64 no.4
• /
• pp.65-72
• /
• 2022

For sustainable development in the construction industry, blast furnace slag has been used as a substitute for cement in concrete. In contrast, ferronickel slag, which is the by-product generated during smelting to ferronickel used in the manufacturing of stainless steel and nickel alloys, has a limitation to use as a binder and an aggregate due to its expansive characteristics. Recently, stabilization technology of ferronickel slag has been improved and studies have been carried out to utilize ferronicke slag as fine aggregate in concrete. Therefore, in this study, basic mechanical properties of concrete used in ferronickel slag aggregate was evaluated. The compressive strength (24, 30, 40 MPa) and replacement rate of ferronickel slag aggregate (0, 10, 25, 50%) were considered as experimental variables. As a result of test, concrete replaced fine aggregate with 25% ferronickel slag aggregate showed superior performance in the compressive strength and flexural strength.

• Restorative Dentistry and Endodontics
• /
• v.43 no.3
• /
• pp.18.1-18.12
• /
• 2018

Objectives: This study compared the retention of BioAggregate (BA; Innovative BioCeramix) and mineral trioxide aggregate (MTA; Angelus) as coronal plugs after applying different intracanal medications (ICMs) used in regenerative endodontic. Materials and Methods: One-hundred human maxillary central incisors were used. The canals were enlarged to a diameter of 1.7 mm. Specimens were divided into 5 groups (n = 20) according to the ICM used: calcium hydroxide (CH), 2% chlorhexidine (CHX), triple-antibiotic paste (TAP), double-antibiotic paste (DAP), and no ICM (control; CON). After 3 weeks of application, ICMs were removed and BA or MTA were placed as the plug material (n = 10). The push-out bond strength and the mode of failure were assessed. The data were analyzed using 2-way analysis of variance, the Tukey's test, and the ${\chi}^2$ test; p values < 0.05 indicated statistical significance. Results: The type of ICM and the type of plug material significantly affected bond strength (p < 0.01). Regardless of the type of ICM, BA showed a lower bond strength than MTA (p < 0.05). For MTA, CH showed a higher bond strength than CON, TAP and DAP; CHX showed a higher bond strength than DAP (p < 0.01). For BA, CH showed a higher bond strength than DAP (p < 0.05). The mode of failure was predominantly cohesive for BA (p < 0.05). Conclusions: MTA may show better retention than BA. The mode of bond failure with BA can be predominantly cohesive. BA retention may be less affected by ICM type than MTA retention.

• Restorative Dentistry and Endodontics
• /
• v.40 no.1
• /
• pp.1-13
• /
• 2015

Advancements in bio-ceramic technology has revolutionised endodontic material science by enhancing the treatment outcome for patients. This class of dental materials conciliates excellent biocompatibility with high osseoconductivity that render them ideal for endodontic care. Few recently introduced bio-ceramic materials have shown considerable clinical success over their early generations in terms of good handling characteristics. Calcium enriched mixture (CEM) cement, Endosequence sealer, and root repair materials, Biodentine and BioAggregate are the new classes of bio-ceramic materials. The aim of this literature review is to present investigations regarding properties and applications of CEM cement in endodontics. A review of the existing literature was performed by using electronic and hand searching methods for CEM cement from January 2006 to December 2013. CEM cement has a different chemical composition from that of mineral trioxide aggregate (MTA) but has similar clinical applications. It combines the biocompatibility of MTA with more efficient characteristics, such as significantly shorter setting time, good handling characteristics, no staining of tooth and effective seal against bacterial leakage.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.9
• /
• pp.1477-1482
• /
• 2007

Gluconacetobacter diazotrophicus strain PA15 exhibited a minimum inhibitory concentration value of 11 mM in an LGI medium amended with $ZnCl_2$. When an LGI medium was amended with Zn metal, solubilization halos were observed in a plate assay, and further solubilization was confirmed in a broth assay. The maximum solubilization was recorded after 120 h with a 0.1% Zn metal amendment. During solubilization, the culture growth and pH of the broth were indirectly correlated. Using a Fourier Transform Infrared Spectroscopy analysis, one of the agents solubilizing the Zn metal was identified as gluconic acid. When the Zn-amended broth was observed under a bright field microscope, long involution cells were observed, and further analysis with Atomic Force Microscopy revealed highly deformed, pleomorphic, aggregate-like cells.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.48 no.7
• /
• pp.25-31
• /
• 2006

Permeable polymer concretes can be applied to roads, sidewalks, river embankment, drain pipes, conduits, retaining walls, yards, parking lots, plazas, interlocking blocks, etc. This study was to explore a possibility of using bottom ash as filler and recycled coarse aggregate of industrial by-products for permeable polymer concrete. The tests carried out at $20{\pm}1^{\circ}C$ and $60{\pm}2%$ relative humidity. At 7 days of curing, unit weight, void ratio, compressive and flexural strength and coefficient of permeability ranged between $1,652{\sim}1,828kgf/m^{3},\;15{\sim}29+%,\;18.2{\sim}24.5\;MPa,\;6.4{\sim}8.4\;MPa\;and\;6.8{\times}10^{-2}{\sim}1.7{\times}10^{-1}\;cm/s$, respectively. It was concluded that the bottom ash and recycled coarse .aggregate can be used in the permeable polymer concrete.

• Geosystem Engineering
• /
• v.21 no.5
• /
• pp.291-296
• /
• 2018

The aim of this work is to study the effect of aggregate type on the physico-mechanical properties and microstructure of bio-mortar (BM). Three different aggregates such as sand, dolomite and basalt were used. BM was prepared by mixing aggregates with bacterial cells (Sporosarcina Pasteurii) and one equimolar (1 M) of $urea/CaCl_2.2H_2O$. The results proved that the chemical composition and physical properties of aggregates play an important role in the microbial precipitation rate as well as size, morphology and crystallinity of the precipitated calcite, which strongly reflects on the properties of the prepared BM. The BM containing dolomite gave the highest compressive strength and lowest water absorption.

• Restorative Dentistry and Endodontics
• /
• v.47 no.2
• /
• pp.18.1-18.9
• /
• 2022

Objectives: This study evaluated alterations in neuronal conductivity related to calcium silicate cements (CSCs) by investigating compound action potentials (cAPs) in rat sciatic nerves. Materials and Methods: Sciatic nerves were placed in a Tyrode bath and cAPs were recorded before, during, and after the application of test materials for 60-minute control, application, and recovery measurements, respectively. Freshly prepared ProRoot MTA, MTA Angelus, Biodentine, Endosequence RRM-Putty, BioAggregate, and RetroMTA were directly applied onto the nerves. Biopac LabPro version 3.7 was used to record and analyze cAPs. The data were statistically analyzed. Results: None of the CSCs totally blocked cAPs. RetroMTA, Biodentine, and MTA Angelus caused no significant alteration in cAPs (p > 0.05). Significantly lower cAPs were observed in recovery measurements for BioAggregate than in the control condition (p < 0.05). ProRoot MTA significantly but transiently reduced cAPs in the application period compared to the control period (p < 0.05). Endosequence RRM-Putty significantly reduced cAPs. Conclusions: Various CSCs may alter cAPs to some extent, but none of the CSCs irreversibly blocked them. The usage of fast-setting CSCs during apexification or regeneration of immature teeth seems safer than slow-setting CSCs due to their more favorable neuronal effects.

• Restorative Dentistry and Endodontics
• /
• v.35 no.3
• /
• pp.152-163
• /
• 2010

This study investigated the changes in gene expression when mineral trioxide aggregate (MTA) was applied in vitro to human dental pulp cells (HDPCs). MTA in a teflon tube (diameter 10 mm, height 2 mm) was applied to HDPCs. Empty tube-applied HDPCs were used as negative control. For microarray analysis, total RNA was extracted at 6, 24, and 72 hrs after MTA application. The results were confirmed selectively by performing reverse transcriptase polymerase chain reaction for genes that showed changes of more than two-fold or less than half. Of the 24,546 genes, 109 genes were up-regulated greater than twofold (e.g., FOSB, THBS1, BHLHB2, EDN1, IL11, FN1, COL10A1, and TUFT1) and 69 genes were down-regulated below 50% (e.g., SMAD6 and DCN). These results suggest that MTA, rather than being a bio-inert material, may have potential to affect the proliferation and differentiation of pulp cells in various ways.

• Bulletin of the Korean Chemical Society
• /
• v.32 no.12
• /
• pp.4171-4175
• /
• 2011

Bio-functionalized gold nanoparticles (AuNPs), which bio-specifically interact with biotin-(strept)avidin, were investigated in this study. AuNPs were functionalized with a synthetically-provided biotin-linked thiol (BLT), which was synthesized by amidation of the active ester of biotin with 2-mercaptoethylamine. The BLT-attached AuNP was bio-specific for streptavidin, making it potentially useful for biosensor applications. To test the bio-specific interactions, the colors, absorption spectra and TEM images were investigated for proteins such as streptavidin, cytochrome C, myoglobin and hemoglobin. The colors and absorption spectra changed when streptavidin was added to the BLT-attached AuNP solution. However, the color and spectra did not change when the other proteins were added to the same solution. These results show that the AuNPs provided a colloidal solution with excellent stability and highly selective absorption characteristics for streptavidin as a target molecule. Proteins were also screened in order to identify a general strategy for the use of optical biosensing proteins based on AuNPs. In addition, TEM images confirmed that streptavidin led the BLT-attached AuNPs to aggregate or precipitate.