• 한국식품영양과학회지
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• 제35권4호
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• pp.493-498
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• 2006

Monascus purpureus MMK2를 이용하여 수용성 적색색소 생산을 위한 배양조건의 최적화에 관한 결과는 다음과 같다. 실험균주가 생산하는 적색색소의 최적 배양조건은 탄소원으로 wheat flour 3.0% 첨가, 질소원으로 $NaNO_3\;0.15%$, 인산염으로 $Na_2HPO_4\;12H_2O$의 농도가 0.25% 및 $MgSO_4$의 농도가 0.15%일 때 가장 높은 적색색소 생성을 나타내었다. 배양온도는 $30^{\circ}C$, 초기 pH가 6.5 및 배양시간 7일일 때 적색색소의 생성능은 모 균주의 생성능인 1.77unit에 비해 23.38배 높은 41.39 unit로 가장 우수한 특성을 조사하였다.

• 환경생물
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• 제26권1호
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• pp.15-21
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• 2008

Ionizing radiation causes many alterations in photosynthetic machineries. However, there is no information about effects of ionizing radiation on the development of photosynthetic machineries in plants. We investigated the greening of etiolated mung bean seedlings after gamma-irradiation of 50 to 300 Gy. The irradiation inhibited seedling growth with great dependence on the radiation dose. In particular, growth of stems was more affected than that of hypocotyls. Irradiated leaves showed inhibition in growth, aberration in morphology, and yellowing in color depending on the radiation dose. Contents of photosynthetic pigments such as chlorophylls and carotenoids were significantly decreased in the irradiated leaves. The apparent electron transport rate for photosynthesis, ETR, was similarly changed depending on the radiation dose. However, the maximal photochemical efficiency of Photosystem II (PSII), Fv/Fm, was little affected by the irradiation. Moreover, the 50-Gy seedlings maintained the control level of light saturating for photosynthesis and showed slightly higher Fv/Fm values in spite of significant decreases in the photosynthetic pigment content and ETR. These results suggest that the inhibition of the overall photosynthetic capacity couldn’t be causally relatqaed with the repression in the initial development of irradiated seedlings and that the overall photosynthetic machineries can develop and work to some extent as a concerted system for photosynthesis even after exposure to acute doses of ionizing radiation.

• BMB Reports
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• 제44권6호
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• pp.405-409
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• 2011

The present study demonstrated that valproic acid (VPA) transcriptionally regulates human GM3 synthase (hST3Gal V), which catalyzes ganglioside GM3 biosynthesis in ARPE-19 human retinal pigment epithelial cells. For this, we characterized the promoter region of the hST3Gal V gene. Functional analysis of the 5'-flanking region of the hST3Gal V gene revealed that the -177 to -83 region functions as the VPA-inducible promoter and that the CREB/ATF binding site at -143 is crucial for VPA-induced expression of hST3Gal V in ARPE-19 cells. In addition, the transcriptional activity of hST3Gal V induced by VPA in ARPE-19 cells was inhibited by SP600125, a c-Jun N-terminal kinase (JNK) inhibitor. In summary, our results identified the core promoter region in the hST3Gal V promoter and for the first time demonstrated that ATF2 binding to the CREB/ATF binding site at -143 is essential for transcriptional activation of hST3Gal V in VPA-induced ARPE-19 cells.

• 생명과학회지
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• 제17권1호
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• pp.137-142
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• 2007

Monascus purpureus KFRI 1134 균주에 대해 UV ($235_{nm}$, 30 second) 조사를 통한 변이주 형성을 유도하여 Monascus sp. MK2-2를 분리하였다. 홍국 천연색소 생산의 최적조건은 rice powder 0.3%와 0.2% yeast extract, 0.3% $NH_4H_2PO_4$의 배지 조성에서 초기 pH 5.0, 배양온도 $30^{\circ}C$ 및 배양기간은 5일이었으며, 홍국 천연색소 생산에 미치는 탄소원의 영향은 wheat flour > rice powder > fructose 순으로 높았다. 질소원은 sodium nitrate > $KNO_3$의 순서로 생성능이 높았으며, C/N ratio가 $17\sim22$일 때 색소의 생성능이 우수하였다. Anti-thrombosis activity 측정결과 Monascus sp. MK2-2 추출물은 aspirin과 유사한 항혈전 기능을 지니고 있음을 알 수 있었다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.205.3-205
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• 2005

• 생약학회지
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• 제51권2호
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• pp.131-138
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• 2020

Ultraviolet (UV)-induced damage plays a major role in ocular diseases, such as cataracts and retinal degeneration. UV irradiation can generate free radicals including reactive oxygen species (ROS), which are known to cause lipid peroxidation of cellular membranes. It has also been shown that UV can damage DNA directly and induce apoptosis. Rhynchosia volubilis Loureiro (the small black bean or yak-kong, RV) and Beta bulgaris (beet, BB) are used as health supplements. In this study, we explored the protective effects of RV and BB against UVA-induced damage in human pigment epithelial (ARPE-19) cells and in mice. RV and BB mixture and their effective constituents (cyanidin, delphidin, petunidin glycosides) improved cell viability and suppressed intracelluar ROS generation. Phosphorylation of p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK), Erk1/2 was analyzed by immunoblotting. RV and BB mixture inhibited UVA-induced phosphorylation of p38 MAPK, JNK, Erk1/2 in APRE-19 cells. RV and BB treatment also showed protective effects on ocular damage in UVA-irradiated mice by increasing the levels of endogenous antioxidants such as superoxide dismutase and glutathione. RV and BB have the potential to be used in a range of ocular diseases and conditions, based on in vitro and in vivo study.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1605-1610
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• 2012

Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of $300{\mu}m$, 0.8% red pepper powder processed as a super fine powder with a vibrational mill ($44{\mu}m$) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% $Na_2CO_3$ either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens.

• 복식문화학회:학술대회논문집
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• 복식문화학회 2003년도 동계 세미나 및 논문 디자인 콘테스트 시상 수상작품 전시회
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• pp.21-26
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• 2003

I 의류의 염색 및 가공 1. 염색 방법 (섬유제품에 의한 분류) ① 섬유 염색 (Fiber Dyeing) ②사염 ③ 원단 염색 ④ 의류 염색 2. 염료 종류에 따른 염색 (Cellulose 섬유를 중심으로) ① 직접 염료에 의한 염색 ② 반응성 염료에 의한 염색 ③ VAT 염료에 의한 염색 ④ Pigment Dyeing ⑤ 형광 증백제에 의한 염색 3. Washing 가공 ① Normal Washing ② Stone Washing ③ Bio Washing ④ Acid Washing ⑤ Sand Washing (중략)

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 2018년도 정기학술대회 발표논문집
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• pp.231-231
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• 2018

• 한국진공학회:학술대회논문집
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• 한국진공학회 2015년도 제49회 하계 정기학술대회 초록집
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• pp.161.2-161.2
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• 2015

Melanin is a black pigment, responsible for hair and skin color. In order to find the melanin stimulatory technique which prove useful for a gray and a white hair-preventive agent or tanning agent, we developed atmospheric pressure plasma jet (APPJ) and tested for tyrosinase activity and melanin production in melanoma (B16F10) cells in vitro. We found plasma dose dependent increase in melanin production. To explore the contributing mechanism in melanin synthesis, intracellular reactive oxygen species (ROS) and MAP kinase signaling pathways were studied. Furthermore, the development of plasma technology for melanin synthesis and planning for in-vivo future studies will be discussed.