• International Journal of Precision Engineering and Manufacturing
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• v.2 no.4
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• pp.30-39
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• 2001

This paper investigates vibrational motion of a flexible beam fixed on a moving cart and carrying a moving mass. The equations of motion of the beam-mass-cart system are analysed through the unconstrained modal analysis. The exact normal mode solution used in modal analysis correspond to the eigenfrequencies for each position of the moving mass and to the ratios of the weight of the beam-mass-car system. Time solutions of normal modes are also transformed properly according to the position of the moving mass. Numerical simulations are carried out to obtain open-loop responses of the system in tracking pre-designed paths of the moving mass. The simulation results show that the model predicts the dynamic behavior of the beam-mass-cart system well. Experiments are carried out to show the validity of the proposed analytical method.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.113-114
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• 2013

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging is a powerful technique for producing chemical images of small biomolecules (ex. metabolites, lipids, peptides) "as received" because of its high molecular specificity, high surface sensitivity, and submicron spatial resolution. In addition, matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) imaging is an essential technique for producing chemical images of large biomolecules (ex. genes and proteins). For this talk, we will show that label-free mass imaging technique can be a platform technology for biomedical studies such as early detection/diagnostics, accurate histologic diagnosis, prediction of clinical outcome, stem cell therapy, biosensors, nanomedicine and drug screening [1-7].

• Analytical Science and Technology
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• v.31 no.3
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• pp.112-121
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• 2018

The hydrothermal carbonization method has received great attention because of the conversion process from biomass. The reaction produces various products in hydrochar, bio-liquid, and gas. Even though its yield cannot be ignored in amount, it is difficult to find research papers on bio-liquid generated from the hydrothermal carbonization reaction of biomass. In particular, the heterogeneity of feedstock composition may make the characterization of bio-liquid different and difficult. In this study, bio-liquid from the hydrothermal carbonization reaction of food wastes at $230^{\circ}C$ for 4 h was investigated. Among various products, fatty acid methyl esters were analyzed using two different extraction methods: liquid-liquid extraction and column chromatography. Different elutions with various solvents enabled us to categorize the various components. The eluents and fractions obtained from two different extraction methods were analyzed by gas chromatography with a mass spectrometer (GC/MS). The composition of the bio-liquid in each fraction was characterized, and seven fatty acid methyl esters were identified using the library installed in GC/MS device.

• Journal of Biosystems Engineering
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• v.43 no.1
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• pp.14-20
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• 2018

Purpose: The purpose of this study was to investigate the performance of a vacuum pyrolysis system, to analyze bio-oil characteristics, and to examine the applicability for farm-scale capacity. Methods: The biomass was pyrolyzed at 450, 480, and $490^{\circ}C$ on an electric heat plate in a vacuum reactor. The waste heat from the heat exchanger of the reactor was recycled to evaporate water from the bio-oil. The chemical composition of the bio-oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Results: According to the analysis, the moisture content (MC) in the bio-oil was approximately 9%, the high heating value (HHV) was approximately 26 MJ/kg, and 29 compounds were identified. These 29 compounds consisted of six series of carbohydrates, 17 series of lignins, and six series of resins. Conclusions: Owing to low water content and the oxygen content, the HHV of the bio-oil produced from the vacuum reactor was higher by about 6 MJ/kg than that of the bio-oil produced from a fluidized bed reactor.

• Journal of Microbiology and Biotechnology
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• v.21 no.8
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• pp.854-857
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• 2011

POMT7, which is an O-methyltransferase from poplar, transfers a methyl group to several flavonoids that contain a 7-hydroxyl group. POMT7 has been shown to have a higher affinity toward quercetin, and the reaction product rhamnetin has been shown to inhibit the formation of beta-amyloid. Thus, rhamnetin holds great promise for use in therapeutic applications; however, methods for mass production of this compound are not currently available. In this study, quercetin was biotransformed into rhamnetin using Escherichia coli expressing POMT7, with the goal of developing an approach for mass production of rhamnetin. In order to maximize the production of rhamnetin, POMT7 was subcloned into four different E. coli expression vectors, each of which was maintained in E. coli with a different copy number, and the best expression vector was selected. In addition, the S-adenosylmethionine biosynthesis pathway was engineered for optimal cofactor production. Through the combination of optimized POMT7 expression and cofactor production, the production of rhamnetin was increased up to 111 mg/l, which is approximately 2-fold higher compared with the E. coli strain containing only POMT7.

• KSBB Journal
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• v.11 no.6
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• pp.704-711
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• 1996

An optimal process design of a foreign protein production system was carried out using a bioprocess flowsheeting software, BioPro Designer, with a capability of economic analysis. The flowsheeting program was applied to a production system of the tailspike protein of Salmonella phage P22, and helped save time and efforts in selecting an optimal process. A wild type tailspike and two types of mutant tailspikes, tsf G244\longrightarrow,R and Su A334\longrightarrowV, were considered in this study to show that the folding characteristics of foreign protein produced inside host influenced the selection of the best production system. An optimal production system for mature tailspike was chosen under the criterion of capital investment per unit mass of mature protein recovered.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.268-274
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• 1998

The mass flow of water in the stem of melon measured by Sap Flow Gauge was compared with the actual flow calculated by the difference between supply and drainage nutrient water to investigate the possibility and accuracy of estimation of melon's transpiration in rockwool culture. The Sap Flow Gauge which was made with copper-constantan theromocouple and nichrome fiber by our research team, was attached to the 3rd node of melon. The outdoor temperature, room temperature, solar radiation and relative humidity were continually measured. The amount of supply and drainage nutrient water were simultaneously measured for calculation of practical consumption of nutrient water to compare with mass flow of sap. The measuring errors of Sap Flow Gauge were 0.3 to 31.8%, which were small at solar radiation of 20MJ.m$^{2}$.d$^{-1}$ . The mass flow of water was lower for the measured value by Sap Flow Gauge than the actual value at higher solar intensity, however it was higher at lower solar intensity The variation of error rate of each Sap Flow Gauge was 0.1 to 13.0%. The measuring error with Sap Flow Gauge was negatively related with solar intensity and temperature. Therefore, to measure more exactly the mass flow of sap for estimation of melon's transpiration, the compensation factor must be calculated.

• 한국연소학회:학술대회논문집
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• 2012.04a
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• pp.235-237
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• 2012

Geodae-Uksae 1 is a variety of Miscanthus sacchariflorus recently discovered in Korea. It is being mass-cultivated for use as energy crop due to its superior productivity, as high as 30 ton/ha/yr for the dry mass. This study investigates the method of producing biochar and bio-oil from the crop using slow pyrolysis. Especially, the study focused on assessing the biochar properties for its application to soil to improve soil quality and sequestrate carbon. Using an electrically heated packed bed reactor, the products of slow pyrolysis from Geodae-Uksae 1 were produced over a temperature range of $300-700^{\circ}C$ with a heating rate of $10^{\circ}C/min$. The biochar, condensable vapor (bio-oil) and residual gases were characterized for the physical and chemical properties. It was concluded that the ideal temperature for pyrolysis to produce biochar is $500^{\circ}C$.

• Imaging Science in Dentistry
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• v.40 no.3
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• pp.149-153
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• 2010

Brown tumor is usually diagnosed at the terminal stage of hyperparathyroidism. Diagnosis of this tumor is confirmed by endocrinologic investigations along with clinical and radiographic examination. Radiographical differential diagnosis of this tumor includes central giant cell granuloma, aneurysmal bone cyst, metastatic tumor, multiple myeloma, and Paget disease. This report presents a rare case of multiple brown tumors occurring at the maxilla and mandible, which was initially misdiagnosed as central giant cell granuloma. Plain radiographs demonstrated multiple well-defined multilocular radiolucency. CT images showed soft tissue mass with low attenuated lesions, perforation of the lingual cortical plate, and a heterogeneous mass at the right thyroid lobe. These findings were consistent with parathyroid adenoma. The patient had hypercalcemia, hypophosphatemia, and elevated alkaline phosphatase level. Surgical excision of the tumor was performed. No recurrence was observed during a 28-month follow-up.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.41-62
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• 1996

Since 1966 tissue culture has been used as a tool for the production of disease indexed stocks from selected plants and their rapid (clonal) mass propagation through the procedure now referred to as micropropagation. The major advantages have been the rapid introduction of new plant cultivars, created within conventional and mutation breeding programmes, as healthy stock for beneficial distribution and the expansion of the world wide horticultural industry. (omitted)