• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.632-633
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• 2005

• 급식외식위생학회지
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• 제4권2호
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• pp.48-54
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• 2023

The study explores the impact of nutritional information on calorie prediction and purchase intent. Administered through self-report questionnaires, the research employs two types of stimuli to assess the influence of nutritional information. Hamburger set images, with or without accompanying nutritional details, were randomly presented to simulate the prevalent mobile app environment for fast-food delivery. Respondents were tasked with predicting calorie content and indicating purchase intent on a Likert 7-point scale. Results show a significant difference in calorie prediction based on the presence of nutritional labeling. While consumers expect positive health effects by estimating more exact calorie information with nutritional labeling, it doesn't significantly influence purchase intent. The study also examines consumer awareness and preferences regarding nutritional labeling policies, emphasizing the need for education and promotion. Most participants recognize the necessity of mandatory nutritional labeling in fast-food establishments, especially through delivery applications, to support healthier choices and prevent obesity.

• 한국차세대컴퓨팅학회논문지
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• 제15권3호
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• pp.16-24
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• 2019

최근 뇌파를 기반으로 한 인간의 감정을 인식하는 연구가 인간-로봇 상호작용 분야에서 활발히 진행되고 있다. 본 논문에서는 MAHNOB-HCI에서 사용된 자기평가와 주석 레이블링 방법과는 다른, 이미지 기반의 뇌파 Topography를 이용한 레이블링을 통해 감정을 평가하는 방법을 제안한다. 제안한 방법은 뇌파 신호를 Topography의 이미지로 변환하여 기계학습 모델을 학습하고 이를 기반으로 Valence 기반의 감정을 평가한다. 제안한 방법은 레이블링 과정을 자동화하여 지연 시간을 없애고 객관적인 레이블링을 제공할 수 있다. MAHNOB-HCI 데이터베이스를 적용한 실험에서 SVM, kNN의 기계학습 모델을 학습하여 주석 레이블링과 성능 비교를 하였으며, 제안 방법의 감정인식 정확도를 SVM에서 54.2%, kNN에서 57.7%로 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제16권9호
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• pp.1429-1433
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• 2006

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

• Mass Spectrometry Letters
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• 제5권3호
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• pp.63-69
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• 2014

In this study, we present a new isotope-coded carbamidomethylation (iCCM)-based quantitative proteomics, as a complementary strategy for conventional isotope labeling strategies, with providing the simplicity, ease of use, and robustness. In iCCM-based quantification, two proteome samples can be separately isotope-labeled by means of covalently reaction of all cysteinyl residues in proteins with iodoacetamide (IAA) and its isotope (IAA-$^{13}C_2$, $D_2$), denoted as CM and iCCM, respectively, leading to a mass shift of all cysteinyl residues to be + 4 Da. To evaluate iCCM-based isotope labeling in proteomic quantification, 6 protein standards (i.e., bovine serum albumin, serotransferrin, lysozyme, beta-lactoglobulin, beta-galactosidase, and alpha-lactalbumin) isotopically labeled with IAA and its isotope, mixed equally, and followed by proteolytic digestion. The resulting CM-/iCCM-labeled peptide mixtures were analyzed using a nLC-ESI-FT orbitrap-MS/MS. From our experimental results, we found that the efficiency of iCCM-based quantification is more superior to that of mTRAQ, as a conventional nonisobaric labeling method, in which both of a number of identified peptides from 6 protein standards and the less quantitative variations in the relative abundance ratios of heavy-/light-labeled corresponding peptide pairs. Finally, we applied the developed iCCM-based quantitative method to lung cancer serum proteome in order to evaluate the potential in biomarker discovery study.

• 한국정보과학회 언어공학연구회:학술대회논문집(한글 및 한국어 정보처리)
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• 한국정보과학회언어공학연구회 2022년도 제34회 한글 및 한국어 정보처리 학술대회
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• pp.35-40
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• 2022

본 논문에서는 시퀀스 레이블링 문제(sequence labeling problem)인 개체명 인식에 사용할 새로운 태깅 포맷인 Delimiter tag (D-tag)를 소개한다. 시퀀스 레이블링 문제에서 사용하는 BIO-tag 포맷은 개체명 레이블을 B (beginning)와 I (inside) 의미의 레이블로 확장하여 타겟 클래스의 수가 2배 증가한다. 또한 BIO-tag 포맷을 사용할 경우, 모델이 B와 I 를 잘못 분류하는 문제가 발생하며, 레이블 수가 많은 세부 분류 개체명의 경우에는 label confusion을 야기한다. 본 논문에서 제안한 D-tag 포맷은 타겟 클래스의 수를 증가시키지 않기 때문에 앞서 언급한 문제를 해결할 수 있다. 실험 결과, D-tag를 사용하여 학습한 모델이 BIO-tag를 사용한 경우보다 더 좋은 성능을 보여, 유망함을 확인하였다.

• 한국식품과학회지
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• 제36권4호
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• pp.677-682
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• 2004

유전자변형(GM)작물의 안전에 대한 논란은 우리나라를 포함한 많은 나라들이 GM 식품의 강제적 표시제를 도입하게 하였다. 본 연구의 목적은 어떤 수준의 열, 압력, 및 산 처리가 정성 PCR과 LEST 방법의 검출 한계 아래까지 GM 콩 혼합물의 DNA나 단백질을 파괴할 수 있는지를 조사하는 것이었다. 결과는 열처리나 고압열처리는 처리 시간에 따라서 도입유전자의 검출이 되지 않을 정도로 DNA와 단백질을 작은 절편들로 파괴함을 보여주었다. 흥미 있는 점은, 가공식품을 위한 LFST의 검출력은 약하게 가공처리된 콩에서 증가하였다. DNA 및 단백질 검출법 모두 $121^{\circ}C$, 1.5기압에서 20분 동안 처리한 후 GM 혼입물의 검출력을 거의 상실하였다. 본 연구의 결과는 시중에 유통되는 어떤 종류의 가공식품이 GM 작물 식품으로 표시되어야 할지를 결정하는데 유용할 것이다.

• Journal of Microbiology and Biotechnology
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• 제26권5호
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• pp.837-845
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• 2016

A novel endodextranase isolated from Paenibacillus sp. was found to produce isomaltotetraose and small amounts of cycloisomaltooligosaccharides with a degree of polymerization of 7-14 from dextran. To determine the active site, the enzyme was modified with 1-ethyl-3-[3-(dimethylamino)-propyl]-carbodiimide (EDC) and α-epoxyalkyl α-glucosides (EAGs), an affinity labeling reagent. The inactivation followed pseudo first-order kinetics. Kinetic analysis and chemical modification using EDC and EAGs indicated that carboxyl groups are essential for the enzymatic activity. Three Asp and one Glu residues were identified as candidate catalytic amino acids, since these residues are completely conserved across the GH family of 66 enzymes. Replacement of Asp189, Asp340, or Glu412 completely abolished the enzyme activity, indicating that these residues are essential for catalytic activity.

• 생물환경조절학회지
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• 제15권2호
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• pp.173-176
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• 2006

The relationship between source leaf position and photo-assimilate translocation and distribution was characterized for tomato (Lycopersicon esculentum Mill) grown in the greenhouse. Three different positions of source leaf on the stem (first node above or below the first fruit cluster and $5^{th}$ node above the first fruit cluster) were tested for their influence on $^{14}CO_2$ assimilation and transfer to different parts of the plant. The leaves at the $5^{th}$ node above the first fruit cluster transferred the highest (57%) proportion of $C^{14}$ to other plant parts, followed by leaves home on the first node below the first fruit cluster (50%), and the first node above the first fruit cluster (39%). In all treatments, fruits served as the strongest sink for $C^{14}$, followed by stem, leaf, and root tissues. The leaf home on the $5^{th}$ node above the first fruit cluster transferred the largest amount of $C^{14}$ to the second fruit cluster.

• Journal of Ceramic Processing Research
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• 제20권2호
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• pp.158-163
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• 2019

Er3+/Yb3+-codoped NaLa(MoO4)2 colloidal nanocrystals were synthesized by pulsed laser ablations in de-ionized water and sodium dodecyl sulfate (NaC12H25SO4, SDS) aqueous solution for up-conversion (UC) luminescence bio-labeling applications. The influences of the SDS molecules on the crystallinities, crystal morphologies, crystallite sizes, and UC luminescence properties of the prepared Er3+/Yb3+-codoped NaLa(MoO4)2 colloidal nanocrystals were investigated in detail. Under a 980-nm excitation, the Er3+/Yb3+-codoped nanocolloidal NaLa(MoO4)2 suspension exhibited a weak red emission near 670 nm and strong green UC emissions at 530 and 550 nm, corresponding to the intra 4f transitions of Er3+ (4F9/2, 2H11/2, 4S3/2) → Er3+ (4I15/2). When the SDS solution was used, a smaller average crystallite size, narrower size distribution, and enhanced UC luminescence were observed. These characteristics were attributed to the amphoteric SDS molecules attached to the positively charged Er3+/Yb3+-codoped NaLa(MoO4)2 colloidal nanocrystals, effectively occupying the oxygen defect on their surfaces. The Er3+/Yb3+-codoped nanocrystalline NaLa(MoO4)2 suspension prepared in the SDS solution exhibited a remarkably strong green emission visible to the naked eyes.