• 아시안잔디학회지
• /
• 제10권4호
• /
• pp.331-339
• /
• 1996

The mass action on the assimilation and dissimilation of a living system from bio-molecules to bio-spheres has been demonstrated by the theoretical models as the bio- and trophic-functions From the viewpoint of this bio-mechanics, the general principle on the pre-equilibrium of the bio-molecular system is found. Key words: Mass action, Living system, Bio-molecule, Bio-sphere, Bio- and trophic function.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제14권7호
• /
• pp.2919-2937
• /
• 2020

Post-Quantum Cryptography (PQC) is rapidly developing as a stable and reliable quantum-resistant form of cryptography, throughout the industry. Similarly to existing cryptography, however, it does not prevent a third-party from using the secret key when third party obtains the secret key by deception, unauthorized sharing, or unauthorized proxying. The most effective alternative to preventing such illegal use is the utilization of biometrics during the generation of the secret key. In this paper, we propose a biometric-based secret key generation scheme for multivariate quadratic signature schemes, such as Rainbow. This prevents the secret key from being used by an unauthorized third party through biometric recognition. It also generates a shorter secret key by applying Principal Component Analysis (PCA)-based Confidence Interval Analysis (CIA) as a feature extraction method. This scheme's optimized implementation performed well at high speeds.

• Journal of Ginseng Research
• /
• 제40권2호
• /
• pp.127-134
• /
• 2016

Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

• Journal of Microbiology and Biotechnology
• /
• 제34권6호
• /
• pp.1249-1259
• /
• 2024

It remains to be determined whether there is a geographical distribution pattern and phylogenetic signals for the Mycena strains with seed germination of the orchid plant Gastrodia elata. This study analyzed the community composition and phylogenetics of 72 Mycena strains associated with G. elata varieties (G. elata. f. glauca and G. elata. f. viridis) using multiple gene fragments (ITS+nLSU+SSU). We found that (1) these diverse Mycena phylogenetically belong to the Basidiospore amyloid group. (2) There is a phylogenetic signal of Mycena for germination of G. elata. Those strains phylogenetically close to M. abramsii, M. polygramma, and an unclassified Mycena had significantly higher germination rates than those to M. citrinomarginata. (3) The Mycena distribution depends on geographic site and G. elata variety. Both unclassified Mycena group 1 and the M. abramsii group were dominant for the two varieties of G. elata; in contrast, the M. citrinomarginata group was dominant in G. elata f. glauca but absent in G. elata f. viridis. Our results indicate that the community composition of numerous Mycena resources in the Zhaotong area varies by geographical location and G. elata variety. Importantly, our results also indicate that Mycena's phylogenetic status is correlated with its germination rate.

• 디지털융복합연구
• /
• 제20권5호
• /
• pp.637-642
• /
• 2022

심전도(ECG, electrocardiogram) 신호를 이용한 본인 인증기술은 기존의 바이오 인증을 대체할 수 있는 본인 인증기술로 주목받고 있다. 디지털 전자키를 인식하는 장치를 차량에 탑재하여 자동차와 무선으로 데이터를 송수신할 수 있게 하고, 스마트폰 등을 활용해 자동차의 차 문을 잠금 또는 해제하거나, 시동을 걸 수 있는 기능을 스마트폰을 통해 차량 제어할 수 있다. 그러나 스마트키는 보안에 취약하여 이를 해결하고 운전자의 편의성을 제공하기 위해 바이오 인증기술을 적용한 스마트키를 연구하였다. 심전도를 이용한 개인인증 알고리즘을 시계 형태의 웨어러블 디바이스에 탑재하여 바이오인증을 하고 개인인증이 완료되면 자동차의 스마트키 기능을 할 수 있었다. 인증율 95%를 달성하였다. 운전자는 스마트키를 소지할 필요가 없고, 분실 및 해킹에 안전하게 보호할 수 있는 대안으로 스마트키를 제안한다. 심전도를 이용한 개인 인증기술을 활용한 스마트키는 개인인증을 통해 다양한 분야에 적용이 가능하고 향후 심전도를 이용한 본인확인 장치 등에 적용할 수 있는 방법을 연구할 계획이다.

• Journal of Microbiology and Biotechnology
• /
• 제19권5호
• /
• pp.439-446
• /
• 2009

The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-S-methyl-O-methyltyrosine (3hSmOmTyr), a nonproteinogenic amino acid, is the precursor of the tetrahydroisoquinoline molecular core. In the biosynthetic gene cluster of SAC/SFM, sacD/sfmD encodes a protein with high homology to each other but no sequence similarity to other known enzymes; sacF/sfmM2 and sacG/sfmM3 encode methyltransferases for C-methylation and O-methylation; and sacE/sfinF encodes a small protein with significant sequence similarity to the MbtH-like proteins, which are frequently found in the biosynthetic pathways of non ribosomal peptide antibiotics and siderophores. To address their function, the biosynthetic cassette of 3h5mOmTyr was heterologously expressed in S. coelicolor and P. putida, and an in-frame deletion and complementation in trans were carried out. The results revealed that (i) SfmD catalyzes the hydroxylation of aromatic rings; (ii) sacD/sacF/sacG in the SAC gene cluster and sfmD/sfmM2/sfmM3 in the SFM cluster are sufficient for the biosynthesis of 3h5mOmTyr; and (iii) the mbtH-like gene is not required for the biosynthesis of the 3h5mOmTyr precursor.

• Journal of Microbiology
• /
• 제45권5호
• /
• pp.422-430
• /
• 2007

Clonostachys rosea (syn. Gliocladium roseum) is a well-known biocontrol agent and widely distributed around the world. In this study, an endochitinase gene Crchi1 was isolated from the mycoparasitic fungus C. rosea using the DNA walking strategy. The Crchi1 ORF is 1,746 bp long and interrupted by three introns. The cloned gene Crchi1 encodes 426 amino acid residues and shares a high degree of similarity with other chitinases from entomopathogenic and mycoparasitic fungi. Several putative binding sites for transcriptional regulation of Crchi1 in response to carbon (5'-SYGGRG-3') and nitrogen (5'-GATA-3') were identified in the upstream of Crchi1. Expression of Crchi1 gene in different carbon sources was analyzed using real-time PCR (RT-PCR). We found that the Crchi1 expression was suppressed by glucose but strongly stimulated by chitin or solubilized components of the cell wall from Rhizoctonia solani. Phylogenetic analysis of chitinases from entomopathogenic and mycoparasitic fungi suggests that these chitinases have probably evolved from a common ancestor.

• BMB Reports
• /
• 제37권4호
• /
• pp.439-444
• /
• 2004

Double stranded targets on the cDNA microarray contain representatives of both the coding and noncoding strands, which will introduce hybridization competition with probes. Here, the effect of double and single strands of targets on the signal intensity and the ratios of Cy5/Cy3 within the same slide were compared. The results show that single stranded targets can increase the hybridization efficiency without changing the Cy5/Cy3 ratio. Based on these results, a new strategy was established by generating cDNA targets with asymmetric PCR, instead of conventional PCR, to increase the sensitivity of the cDNA microarray. Furthermore, the feasibility of this approach was validated. The results indicate that the cDNA microarray system based on asymmetric PCR is more sensitive, with no decrease in the reliability and reproducibility as compared with that based on conventional symmetric PCR.

• Carbon letters
• /
• 제26권
• /
• pp.66-73
• /
• 2018

We assessed the effects of combining bio-char with straw residue mulching on the loss of soil soluble nutrients and citrus yield in sloping land. The two-year study showed that straw residue mulching (ST) and bio-char application combined with straw residue (ST+BC) can significantly reduce soil soluble nutrient loss when compared with the control treatment (CK). The comparative volume of the soil surface runoff after each of the treatments was as follows: CK > ST > ST + BC. Compared with the CK, the runoff volume of the ST was reduced by 13.6 % and 8.5 % in 2014 and 2015, respectively. Compared with the CK, combining bio-char with the ST application reduced the loss of soluble nitrogen and improved the soil total nitrogen content reaching a significant level in 2015. It dramatically increased the soil organic matter content over the two year period (36.3% in 2014, 50.6% in 2015) as well as the carbon/nitrogen ratio (C/N) (16.6% in 2014 and 39.3% in 2015). Straw mulching combined with bio-char showed a trend for increasing the citrus yield.

• 한국약용작물학회지
• /
• 제15권5호
• /
• pp.346-351
• /
• 2007

An efficient somatic embryogenesis and plant regeneration protocol was developed for Schisandra chinensis Baill, using embryogenic cell suspensions and optimized media conditions. Friable embryogenic callus was induced from cotyledonary leaf and hypocotyl explants of 7 days old seedlings on MS agar medium supplemented with 1.0 to $4.0\;mg\;l^{-1}$ of 2,4-dichlorophenoxyacetic acid (2,4-D). Fast growing and well dispersed embryogenic cell suspensions were developed within two months when embryogenic calli were transferred to MS liquid medium containing $1.0\;mg\;l^{-1}\;2,4-D$. One third strength of MS medium was the best for both overall growth and development of somatic embryos in liquid culture. Over 3400 viable somatic embryos were produced from each 150 ml flask with an initial cell density of 30 mg in 30 ml medium. Germinated somatic embryos developed in liquid medium converted into plantlets after transferred to half-strength MS semi-solid medium. Approximately 90% of the converted plantlets were successfully transplanted to soil and grew into fertile plants.