Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Diverse Mycena Fungi and Their Potential for Gastrodia elata Germination

  • Xiao-Han Jin (State Key Laboratory Conservation and Utilization of Bio-Resources in Yunnan) ;
  • Yu-Chuan Wang (Gastrodia Tuber Research Institute of Zhaotong) ;
  • Dong Li (State Key Laboratory Conservation and Utilization of Bio-Resources in Yunnan) ;
  • Yu Li (State Key Laboratory Conservation and Utilization of Bio-Resources in Yunnan) ;
  • Hai-Yan He (The Agriculture and Life Sciences College, Zhaotong University) ;
  • Han-Bo Zhang (State Key Laboratory Conservation and Utilization of Bio-Resources in Yunnan)
  • Received : 2024.01.10
  • Accepted : 2024.04.18
  • Published : 2024.06.28
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Abstract

It remains to be determined whether there is a geographical distribution pattern and phylogenetic signals for the Mycena strains with seed germination of the orchid plant Gastrodia elata. This study analyzed the community composition and phylogenetics of 72 Mycena strains associated with G. elata varieties (G. elata. f. glauca and G. elata. f. viridis) using multiple gene fragments (ITS+nLSU+SSU). We found that (1) these diverse Mycena phylogenetically belong to the Basidiospore amyloid group. (2) There is a phylogenetic signal of Mycena for germination of G. elata. Those strains phylogenetically close to M. abramsii, M. polygramma, and an unclassified Mycena had significantly higher germination rates than those to M. citrinomarginata. (3) The Mycena distribution depends on geographic site and G. elata variety. Both unclassified Mycena group 1 and the M. abramsii group were dominant for the two varieties of G. elata; in contrast, the M. citrinomarginata group was dominant in G. elata f. glauca but absent in G. elata f. viridis. Our results indicate that the community composition of numerous Mycena resources in the Zhaotong area varies by geographical location and G. elata variety. Importantly, our results also indicate that Mycena's phylogenetic status is correlated with its germination rate.

Keywords

Acknowledgement

This work was supported by Open Research Program of State Key Laboratory for Conservation and Utilization of Bio-Resource in Yunnan (2022KF002), National Natural Science Foundation of China (31960288), Special Funds for Central Guidance of Local Scientific and Technological Development (202307AB110011) and Innovative Research Foundation for Graduate Students of Yunnan University (KC23235551). Special thanks to Cheng-Shuai Zou (a G. elata farmer in Zhaotong, China) for providing G. elata seeds for this experiment.

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