• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제5권6호
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• pp.267-272
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• 2016

생물정보학분야에서 현미경을 통해 얻은 세포 영상은 생물학적 정보를 얻기 위한 중요한 지표이다. 연구자들은 영상을 육안으로 분석하기 때문에 분석에 많은 시간과 고도의 집중력이 요구된다. 게다가 연구자의 주관적 관점이 분석에 개입되어 결과를 객관적으로 정량화하는데 어려움이 있다. 따라서 본 연구에서는 OpenCV 라이브러리를 이용하여 세포의 자동 분석을 위한 HCS(High Content Screen) 알고리즘을 개발하였다. HCS 알고리즘은 이미지 전처리 과정, 세포 계수, 세포 주기와 분열지수 분석 기능을 포함한다. 본 연구에서는 공초점 레이저 현미경을 통해 얻은 위암세포(MKN-28) 영상을 분석에 사용하였으며, 성능 평가를 위해 세포영상 분석 프로그램인 ImageJ와 전문 연구원의 세포 계수 분석결과를 비교하였다. 실험 결과 HCS 알고리즘의 평균 정확성이 99.7%로 나타났다.

• 농촌계획
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• 제23권4호
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• pp.49-59
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• 2017

The importance of information system evaluation has increased according to ICT development and this evaluation is required for objective verification. The aim of this study is to develop evaluation indicators for web-based information system and verify the availability of these evaluation indicators through applying to agricultural water integrated information system. This study finds and provides eight evaluation items and 64 indicators for a web-based information system using $3{\times}3$ Mandal-Art matrix that is a tool for creating creative ideas. These evaluation items are design, contents, navigation, stability, community, convenience, usefulness, and accuracy. Total 64 evaluation indicators were presented by deriving eight evaluation items for each using $9{\times}9$ Mandal-Art matrix. When evaluating information system using evaluation indicators, it can be identified the vulnerable items in the information system. Also, the comprehensive results of the information system could be understood when appearing a single score after weighting. In addition, it can also help to prepare a questionnaire for evaluation systematically.

• 농촌계획
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• 제25권1호
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• pp.51-65
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• 2019

This study developed diagnostic system to understand the level of welfare cultural service and infrastructure in rural areas. The applicability was reviewed through the Delphi survey and the sample survey of 60 villages. The diagnostic indicators consist of three areas: the demand area, supply area, and delivery area. The demand area consists of 8 indicators, 25 indicators for welfare services and infrastructure (healthcare, social welfare) and 32 indicators for cultural services and infrastructure (culture, education, leisure sports). The service delivery area was divided into service supply area access and traffic accessibility (public transport use status and rural transport model status) by each indicator. A diagnostic system was applied to 60 villages. Services and infrastructure for rural welfare and culture were supplied more in the Si area than Gun area. The delivery area was easier to access the Gun area than Si area. In the case of traffic access, public transportation was more frequently used in the Si area than Gun area, and the rural transportation model was found to have a relatively large amount in the Gun area compared to Si area. The diagnosis system about services and infrastructure for rural welfare and culture will provide information necessary for establishment and decision making of regional development policy taking into account characteristics of rural areas in the future.

• 식품보건융합연구
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• 제5권3호
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• pp.35-40
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• 2019

In this study, general bacterial counts and coliform counts, which are hygienic indicator microorganisms, were tested for candy, chocolate, and jelly which are easily available and enjoyed around. After dropping each sample on the desk, indoors, and outdoors, it is immediately collected, or washed and collected to confirm the myth of the 3-second rule. Immediately after removing the wrapping paper, each sample was dropped on the desk, indoors, and outdoors, and after 3 seconds from the moment of contact with the surface, and then collected in a sample bag using sterilized sanitary gloves. After the same operation, each sample was rinsed for 5 seconds using sterilized sanitary gloves and sterilized distilled water, and then collected in a sample bag. The number of bacteria detected in non-washing candies was 41 CFU/g at outdoor and the number of bacteria detected in non-washing chocolate was 76 CFU/g at outdoor. The number of bacteria detected in non-washing jellies was 79 CFU/g at outdoor. Coliform group was not detected in all samples. This showed good results at the level of m = 10,000 or less, which is an allowable value suggested in the Food Code. Also, effect of washing on contaminated food was confirmed. This result is remarkably low compared with the microorganism specimens shown in Food Code, and it is confirmed that contamination occurs but not high value. Therefore, the myth of the 3-second rule is true compared to the figures based on Food Code. However, it showed the characteristics of bacteria that could survive and cross-contaminate on dry food surfaces and emphasized the importance of hygiene through food contact to unsanitary surfaces to minimize the risk of food poisoning.

• Journal of Species Research
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• 제10권3호
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• pp.246-254
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• 2021

Subalpine and alpine ecosystems are especially vulnerable to temperature increases. Betula ermanii Cham. (Betulaceae) is a dominant broad-leaved tree species in the subalpine zone and is designated as a 'Climate-sensitive Biological Indicator Species' in South Korea. This study aimed to predict the potential distribution of B. ermanii under current and future climate conditions in South Korea using the MaxEnt model. The species distribution models showed an excellent fit (AUC=0.99). Among the climatic variables, the most critical factors shaping B. ermanii distribution were identified as the maximum temperature of warmest month (Bio5; 64.8%) and annual mean temperature (Bio1; 20.3%). Current potential habitats were predicted in the Baekdudaegan mountain range and Mt. Hallasan, and the area of suitable habitat was 1531.52 km², covering 1.57% of the Korean Peninsula. With global warming, future climate scenarios have predicted a decrease in the suitable habitats for B. ermanii. Under RCP8.5-2070s, in particular, habitat with high potential was predicted only in several small areas in Gangwon-do, and the total area suitable for the species decreased by up to 97.3% compared to the current range. We conclude that the dominant factor affecting the distribution of B. ermanii is temperature and that future temperature rises will increase the vulnerability of this species.

• 한국미생물·생명공학회지
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• 제49권2호
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• pp.157-166
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• 2021

The probiotic market is constantly continuing to grow, concomitantly with a widening in the range and diversity of probiotic products. Probiotics are defined as live microorganisms that provide a benefit to the host when consumed at a proper dose; the viability of a probiotic is therefore of crucial importance for its efficacy. Many products undergo lyophilization for maintaining their shelf-life. Unfortunately, this procedure may damage the integrity of the cells due to stress conditions during both the freezing and (vacuum-) drying process, thereby impacting their functionality. We propose a lysine-based mixture for rehydration of freeze-dried probiotics for improving their viability during in vitro simulated gastric and duodenum stress conditions. Measurement of the zeta potential served as an indicator of cell integrity and efficacy of this mixture, while functionality was estimated by adhesion to a human enterocyte-like Caco-2 cell-line. The freeze-dried bacteria exhibited a significantly different zeta potential compared to fresh cultures; however, this condition could be restored by rehydration with the lysine mixture. Recovery of the surface charge was found to influence adhesion ability to the Caco-2 cell-line. The optimum lysine concentration of the formulation, designated "Zeta-bio", was found to be 0.03 M for improving the viability of Lactiplantibacillus plantarum Lp-115 by up to 13.86% and a 7-strain mixture (400B) to 41.99% compared to the control rehydrated with distilled water. In addition, the lysine Zeta-bio formulation notably increased the adherence ability of lyophilized Lp-115 to the Caco-2 cell-line after subjected to the in vitro stress conditions of the simulated gastrointestinal tract passage.

• 농촌계획
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• 제16권3호
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• pp.159-171
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• 2010

Since rapid industrialization of Korean society, out-of-village roads have been greatly improved, while almost all of in-village roads have not been fundamentally improved yet. Unless village roads should be improved in relatively comparison with trunk roads, it has been widely recognized that grass rooted achievement of accessibility revolution not be realized. In this regard, this study tried to develop evaluation indicators system for improvement of village roads. The evaluation indicator system on village road conditions was proposed which is sub-categorized as structural improvement of road itself, its communication serviceability and public securing level of property rights of road site. The system has 6 indicators(2 of each subcategory);good pavement ratio and over 3m wide road length ratio, ratio of household fronting under 2m wide road and connectivity of road network, ratio of registered as 'road' in land category and ratio of publicly owned road sites. In the final conclusion, village roads in rural Korea have been generally in worse condition regardless of whether prior improvement works or not, except some of recent plan-based improvement villages.

• 대한한의진단학회지
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• 제10권1호
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• pp.46-53
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• 2006

Magnetocardiography provides non-invasive three-dimensional electroanatomical imaging of cardiac electrophysiology. The aim of this study is to find the index for using the indicator of magnetocardiography. An object of this study is 214 papers registered on www.pubmed.com. The index used in magnetocardiography is the interval, morphology, amplitude and integrals of QRS complex, P wave, T wave. The current density and electric field map are used, too. A new try of the index is LAS, W-HEAD, Relative Smoothness index(RS index) etc.

• 농촌계획
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• 제20권2호
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• pp.1-10
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• 2014

Smartphones change the picture of data and information sharing and make it possible to share various real-time flooding data and information. The vulnerability indicators of farmland inundation is needed to calculate the risk of farmland flood based on changeable hydro-meteorological data over time with morphologic characteristics of flood-damaged areas. To find related variables show the vulnerability of farmland inundation using the binary-logit model and correlation analysis and to provide vulnerability indicators were estimated by fuzzy set method. The outputs of vulnerability indicators were compared with the results of Monte Carlo simulation (MCS) for verification. From the result vulnerability indicators are applicable to mobile_based information system of farmland inundation.

• Agricultural and Biosystems Engineering
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• 제2권2호
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• pp.59-62
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• 2001

The starch content of apples, which is related to the maturity and ripeness, was quantified by using a computer vision system. The stained area was measured by image analysis when an apple slice was stained with KI/I$_2$ solution. The ratio of stained area of the apple slice to its whole area, so called the starch index, was defined as an indicator of the starch content. When Tsugaru apples were manually sorted into immature, turing and mature groups, their starch indices were 0.374, 0.312 and 0.129, respectively. The starch index had relationships to various physicochemical properties of Tsugaru apples. At the statistically significant level of 0.1%, it was correlated with the pH value, bio-yield force, rupture force and color of intact and skin-removed apples. At the 1% significant level, it had the correlation with the density and moisture content.