• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.146.2-146.2
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• 2014

KIST 6MV 가속기는 이온빔 분석 그리고 가속기 질량 분석법(Accelerator mass spectrometry)으로 활용된다. 이온빔 분석으로는 RBS, TOF ERD, PIXE. ${\mu}$-Probe을 할 수 있으며 AMS(Accelerator mass spectrometry)는 액체섬광측정법(LSC)과 비교할때 민감도는 1,000배 정도로 3H, 14C, 26Al,41Ca 을 10-21 ~ 10-18 mole/mg 까지 검출 가능하여. 응용분야로는 BAMS(Biological AMS), 전통과학, 지구과학, 환경과학에 활용되고 있다. 이중 AMS의 생-의학분야(BAMS)의 응용은 최근 매우 중요하게 연구되고 있다. BAMS의 활용 연구에 사용하는 핵종으로는 주로 3H, 14C, 41Ca, 36Cl를 사용하며, 14C 화합물은 쉽게 구할 수 있고, 자연방사선 이하의 낮은 14C labeled drug 사용하기 때문에 1948년 이후 생물학 연구에 혁신적으로 활용되고 있다. 주 활용분야로는 (1) 신약개발은 임상실험 전(Phase 0) 이용되며, 14C로 표지된 bio-molecule을 자연수준의 방사선 농도에서 추적자로 사용하여 질량을 측정하는 방법을 활용. (2) 의과학분야는 인체 내에서의 추적자 연구수행 (3) 항암제 연구는 암조직 중 약물농도와 암효과의 상관성을 연구 (4) 바이오 기술 분야에서는 생약 유효물질의 체내 대사연구 등을 할 수 있어 전세계적으로 활발히 연구가 진행되고 있다. KIST에서는 6MV가속기를 BAMS 연구에 활용하기 위하여 전처리 단계의 Combustion, Gas transfer, Reduction 등을 자체 제작하여 테스트 중에 있으며, BAMS 샘플의 Gas는 호기중의 성분, 대기 성분이 있으며, Liquid는 혈액(혈장,혈청,적혈구), Solid는 DNA, 세포, 장기 뼈, 피부, 식물조직, 사료, Drug 및 그 대사 류가 있다. AMS 측정 결과는 14C/12C 비율로 나타나며 그 결과를 농도로 환산하여 분석하게 된다. 또한 분석 데이터 신뢰를 확보하기 위하여 표준시료 및 품질관리용 시료를 사용하여 BAMS분석법에 대한 검증을 실시하였다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.3
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• pp.192-196
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• 2004

The in vitro effect of bisphenol A (BPA) on ovarian steroidogenesis of the longchin goby (Chasmichthys dolichognathus) was investigated. Oocytes taken during the maturing phase (vitellogenic, fully vitellogenic or germinal vesicle breakdown stage) were incubated with BPA (100 ng/mL) in the presence of exogenous precursor $^{3}H-17\alpha\;hydroxyprogesterone\;(^{3}H-17\alphaOHP).$ Steroids were extracted from the media and the isolated oocytes, and the extracts were separated and identified by thin layer chromatography and gas chromatography-mass spectrometry. The identities of the major metabolites were progestogens $[17{\alpha}-hydroxy,20{\alpha}-dihydroprogesterone\;(17{\alpha}20{\alpha}OHP)\;and\;17{\alpha}-hydrxy,20{\beta}-dihydroprogesterone\;(17{\alpha}20{\beta}OHP),$ androgens [androstenedione (A4) and testosterone (T)] and estrogens [estrone $(E_1)\;and\;estradiol-17{\beta}(E_2)].$ BPA treatment inhibited production of estrogens in all the maturing phases and progestogens in the germinal vesicle migrating stage. Percentage yield of estrogens was decreased with increased yield of androgens. In conclusion, BPA had an inhibitory effect on the conversion of $^3H-17\alphaOHP$ to estrogens and progestogens. These results demonstrate that BPA can act either estrogenic or anti-estrogenic effects.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.74-79
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• 2008

The aim of this study was to analyze the chemical composition of 14 kinds of citrus oils and to test their biological activities. Citrus essential oils were obtained by steam distillation from immature fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Limonene (55.4% to 91.7%), myrcene (2.1% to 32.1%), ${\alpha}$-pinene (0.6% to 1.6%) and linalool (0.4% to 6.9%) were the major components in most citrus species. To evaluate in vitro antibacterial activity, all essential oils were tested against Propionibacterium acnes and Staphylococcus epidermidis. Nine out of fourteen citrus oils exhibited antibacterial activity against P. acnes, but not against S. epidermidis. The effects of the citrus oils on DPPH radical scavenging, superoxide radical anion scavenging, nitric oxide radical, and cytotoxicity were also assessed. Three essential citrus oils, Joadeung, Dongjunggyul, and Bujiwha, exhibited potent inhibitory effects on nitric oxide production. Two essential oils, Dongjunggyul and Joadeung, showed potent free radical scavenging activities in the DPPH assay. For future applications in cosmetic products, we also performed MTT assays in a human dermal fibroblast cell line. The majority of the essential oils showed no cytotoxicity. The results indicate that citrus essential oils can be useful natural agents for cosmetic application.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.4
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• pp.86-96
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• 2012

Hydrogen gas production of anaerobic fermentative process from food waste as a substrate was 3.47 mg $H_2/g$ COD. The hydrogen production was little less than the synthetic wastewater with sucrose as a substrate (7.56 mg $H_2/g$ COD). The B/A ratios of the synthetic wastewater and food waste were 3.73 or 8.01 respectively. Butyric acid was more produced when hydrogen production was higher. Microbial community in the samples was analyzed as Escherichia sp., Klebsiella sp., Clostridium sp., Bacterium sp., and Enterobacter sp. Clostridium sp. was detected both samples but Klebsiella sp. was more active with fermentation process of the food waste. Taxonomic description shows that 60% of the microorganism was ${\gamma}-proteobacteria$ and Firmicute and Bacteria was 20% respectively.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.16-16
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• 2011

Plasmas in saline solutions receive considerable attention in recent years. How the operating parameters influence the plasma characteristics and how the electrode erosion occurs have been topics that require further study. In the first part of this talk, the effect of the frequency on the plasmas characteristics in saline solution driven by 50~1000 Hz AC power will be presented. Two distinct modes, namely bubble and jetting modes, are identified. The bubble mode occurs under low frequencies. In this mode, one mm-sized bubble is tightly attached to the electrode tip and oscillates with the applied voltage. With an increase in the frequency, it shows the jetting mode, in which many smaller bubbles are continuous formed and jetted away from the electrode surface. Multiple mechanisms that are potentially responsible to such a change in bubble dynamics have been proposed and the dominant mechanism is identified. From the Stark broadening of the hydrogen optical emission line, electron densities in both modes are estimated. It shows clearly that the driving frequency greatly influences the bubble dynamics, which in turn alters the plasma behavior. In the second part, the study of the erosion of a tungsten electrode immersed in saline solution under conditions suitable for bio-medical applications is presented. The electrode is immersed in 0.1 M saline solution and is positively or negatively biased using a DC power source up to 600 V. It is identified that when the electrode is positively biased, erosion by the surface electrolytic oxidation is the dominant mechanism with an applied voltage below 150 V. An increase in the applied voltage leads to the formation of the plasma and the damage by the plasma and the thermal effect becomes more prominent. The formation of the gas film at the electrode surface leads to the formation of the plasma and hinders the electrolytic erosion. In the negatively-biased electrode, no electrolytic oxidation is seen and the damage is mostly likely due to the plasma erosion and the thermal effect.

• Food Science and Biotechnology
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• v.16 no.6
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• pp.975-980
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• 2007

Determination of ethyl carbamate content in imported alcoholic beverages in Korea and an exposure assessment were conducted. In gas chromatography/mass spectrometry/selected ion monitoring (GC/MS/SIM) analysis, 2.5-39, 8-263, 6.3-112, 11.3-23.5, 53-94, 8.5-38.5, 7-9.5, 21.3-31.5, 5-832.5, and $10.5-364.8\;{\mu}g/L$ of ethyl carbamate were detected in imported beers, sakes, whiskies, vodkas, Chinese liquors, cognacs, tequilas, rums, liqueurs, and wines, respectively. The exposure assessment indicated that the exposure of Korean adults to ethyl carbamate were lower than 20 ng/kg BW per day, (the virtual safe dose) indicating that the amount of ethyl carbamate exposed through fermented food and alcoholic beverages including imported products are currently in the 'no significant risk level'. However, the present low exposure to ethyl carbamate through the imported alcoholic products was not due to the low contents of ethyl carbamate in imported products, but low consumption of the imported products. Therefore, given increasing importation of alcoholic beverages in Korea, reductions of ethyl carbamate content in imported alcoholic beverages, especially non-distilled products, should be required by regulating limits on the ethyl carbamate content in the imported alcoholic beverages.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.1
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• pp.84-89
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• 2003

The purpose of this study was to evaluate and improve the pre-treatment facility for foodwaste and sewage sludge mixture treatment. The process of foodwaste pre-treatment consists of storage, classification with crushing, and thickening. The effluent of sewage treatment facility was used as the diluting and washing water. The panicle size of foodwaste after pre-treatment was almost under 2mm, the mixture of foodwastes and sewage sludge showed an advantage to the anaerobic digestion. The amount of gas production increased from 0.8ton/day ($CH_4$ : 0.5ton/day) to 3.5ton/day ($CH_4$ : 2.3ton/day) after the anaerobic digestion of the foodwastes and sewage sludge mixture. The amount of sludge cake increased from 11.2ton/day to 21.2ton/day. Therefore, the proper operation of the foodwaste pre-treatment facility was contributed to the efficient anaerobic digestion of foodwaste and sewage sludge mixture treatment.

• Journal of Soil and Groundwater Environment
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• v.19 no.1
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• pp.1-7
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• 2014

When processing the biomass by Hydrothermal carbonization (HTC), a slow pyrolysis process, it produces bio-gas, biooil, and biochar. Among these end products, biochar is known for isolating or storing carbon and being used as a soil amendment. In this study, the characteristics of biochar generated by HTC at $250^{\circ}C$ for 1 hour, 2 hours, 3 hours, and 20 hours with synthetic food wastes and wood wastes were analyzed for potential uses in soil contaminated with heavy metals. The yield of biochar (weight %) increased when the ratio of wood wastes increased and showed a decreasing tendency as reaction time increased. Elemental analysis of biochar based on various conditions showed a maximum of 70% carbon (C) content. The carbon content showed an increasing tendency with the increase of wood wastes. Iodine adsorption test was peformed to determine the optimum reaction condition, which was 15% wood waste for mixing ratio and 2 hours for reaction time. Using biochar generated at the optimum condition, its capability of adsorbing heavy metals (Cd, Cu, Pb, Zn, Ni) was evaluated. It was concluded that lead (Pb) was removed efficiently while zinc (Zn) and nickel (Ni) were hardly adsorbed by biochar.

• Journal of the Korean Wood Science and Technology
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• v.35 no.1
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• pp.1-10
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• 2007

This study was reviewed on the bioethanol production from biomass resources and feedstock supply in America. U.S. Department of Energy (USDE) and the u.s. Department of Agriculture USDA) are both strongly committed to expand the role of biomass as an energy source. They support biomass fuels and products as a way to reduce the need for oil and gas imports, to strengthen the nation's energy security and environmental quality. And it was envisioned a 20 percent replacement of the current U.S.transportation fuel consumption in 2030. Also it was reviewed policies to encourage the expanding of Bio-based fuel use to replace gasoline, such as Clean Air Act, Federal Clean Fuel Program and American Jobs Creation Act. In feedstock supply it was assumed forest biomass will be supplied in 368 million dry tons yearly and the agriculture derived biomass adopted by new technologies and land use change will be supplied in 998 million dry tons, including highly 818 million dry tons of lignocellulosic biomass such as perenial crops (hybrid trees, grasses) corn stover, other crop residues. This amount is 5 times to the amount from based current agricultural technology and crop land.

• Journal of The Korean Society of Agricultural Engineers
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• v.61 no.6
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• pp.21-30
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• 2019

Livestock odor is comprised of mixed type of odorous compounds. Among these, ammonia ($NH_3$) and hydrogen sulfide ($H_2S$) are the two known major odor causing substances. Because high odor concentration reduces productivity of livestock and causes damage to the surrounding communities, quantitative analysis is needed to manage the odor inside and outside the livestock facilities. It is also necessary to evaluate odor dispersion according to the distance between the receptors taking into account the influence of odor source and weather condition. Therefore, in this study, we tried to evaluate the internal environment and odor dispersion from experimental pig house considering weather conditions. An experimental farm was specifically selected to eliminate the interference of odors generated by adjacent farms. $NH_3$ and complex odor were quantitatively analyzed using a gas detector and air dilution sensory method. The concentration of $NH_3$ and complex odor in pig house showed a distinct concentration difference according to the cleaning and ventilation conditions. $NH_3$ concentration and complex odor was lower than emission standard in the pig house and at the site boundary. The average $NH_3$ concentration (P1~P3) and the $NH_3$ concentration at the site boundary (S1) were strongly correlated with R=0.77. While the correlation for complex odor inside and at the site boundary had R=0.52. The correlation coefficient between $NH_3$ and the complex odor was 0.80.