• Biomolecules & Therapeutics
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• v.18 no.2
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• pp.171-177
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• 2010

Appetite is inhibited by the anorexigenic neuropeptides POMC (proopiomelanocortin) and CART (cocaine-amphetamine-regulated transcript) in the hypothalamus. The present study was performed to examine the inhibitory effects of baicalin against food intake and the upregulation of POMC/CART. Short-term food intake (48 h) was significantly inhibited by treatment with baicalin (10 mg/kg, p<0.05) in C57BL/6 mice. Immunohistochemical analysis showed that baicalin upregulated POMC and CART levels in the arcuate nucleus of the hypothalamus. These effects were also examined using an in vitro system. pPOMC-Luc or pCART-Luc plasmids were transformed into mouse N29-2 neuronal and human SH-SY5Y cells, and the activities of baicalin were examined in these cells. Baicalin increased POMC and CART promoter-driven luciferase activity in a dose-dependent manner without cytotoxic effects. These results suggest that baicalin downregulates short-term food intake while upregulating POMC and CART expression.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.2
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• pp.133-139
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• 2014

Veterinary antibiotics (VAs) in animal compost and organic fertilizer can have adverse effect on ecosystem and eventually human health. The main purpose of this research was to evaluate feasibility of Charm II system for monitoring residuals of VAs in animal compost and organic fertilizer. Four different VAs (Tetracyclines: TCs, Sulfonamides: SAs, Macrolides: MLs, and ${\beta}$-lactams: ${\beta}$-LTs) were analyzed and total of 100 samples were monitored. Results reveled that SAs in animal compost showed the highest detection frequency (64%) with exceeded concentration of criteria. However, very low detection frequency (0-12%) for ${\beta}$-LTs was observed in animal compost and organic fertilizer. Depending on physicochemical properties of each VAs, detection frequency of VAs was determined. In conclusion, charm II system can be utilized to screen if residual of VAs is in animal compost and organic fertilizer. Also, more research is necessary to establish standard method for analysis of VAs in complex matrix and to minimize adverse effect of VAs from source to environment.

• Journal of Ecology and Environment
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• v.41 no.3
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• pp.99-106
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• 2017

Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional "Jopoong" plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.

• Transactions of the Korean hydrogen and new energy society
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• v.28 no.4
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• pp.426-432
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• 2017

Hydrothermal carbonization (HTC) is an effective and environment friendly technique; it possesses extensive potential towards producing high-energy density solid fuels. it is a carbonization method of thermochemical process at a relatively low temperature ($180-250^{\circ}C$). It is reacted by water containing raw material. However, the production and quality of solid fuels from HTC depends upon several parameters; temperature, residence time, and pressure. This study investigates the influence of operating parameters on solid fuel production during HTC. Especially, when food waste was reacted for 2 hours, 4 hours, and 8 hours at $200^{\circ}C$ and 2.0-2.5 MPa, Data including heating value, proximate analysis and water content was consequently collected and analyzed. It was found that reaction temperature, residence time are the primary factors that influence the HTC process.

• Applied Chemistry for Engineering
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• v.26 no.5
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• pp.621-623
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• 2015

This article describes a purification method yielding high purity of MFB produced from DMT production process. Aldehyde functional group of MFB included in side-products were converted to acetal compound via reacting with methanol and further separated. Hydrolysis process of the acetal product was continued under acidic condition and highly pure MFB were obtained with 90% yield. The structure of MFB was analyzed by $^1H$ NMR and $^{13}C$ NMR spectroscopy. Also, the purity of MFB was estimated to be over 99% by GC analysis.

• Journal of Biosystems Engineering
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• v.32 no.3
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• pp.190-196
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• 2007

The purpose of this study is to develop a measurement system of cutter conditions for combine header diagnosis during rice harvesting. A load cell was installed at the locker-arm to measure load fluctuation and an acceleration senor was used to monitor vibration signal of cutter bar. The data were collected from a paddy field during harvesting. The tests were conducted with a normal cutter, a loosened cutter, a broken cutter, and a worn-out connecter pin at the field. The vibration signals converted by FFT (Fast Fourier Transformation), filtered, and normalized. The load data and peak values of vibration signals in four different frequency ranges were used to determine the cutting operation and the cutter conditions of combine. The multiple comparison tests showed that the load data and peak values of vibration signals were important to monitor the cutting operation and cutter conditions of combine header.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.1
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• pp.87-89
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• 2001

The nucleotide sequence of the domestic silkworm (Bombyx mori) mitochondrial (mt) control region and its flanking genes was determined from PCR clones. The control region of the silkworm mt genome was located between the small ribosomal RNA gene and transfer RN $A^{Met}$. This 499 bp control region hale 95.4% A+T content. Extensive comparative analysis studies performed with similar control region of other insect genomes could not reveal a highly conserved region containing conserved motifs of animal mito-chondrial genome. The remarkable feature that found in this control region was the presence of tandem motifs containing nine repetitive sequences. The potential usefulness of this motif sequences for Bombyx species or their taxonomically related species is enhanced by its unique localization in the maternally inheritance mitochondrial molecule.e.

• Journal of Korean Society on Water Environment
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• v.23 no.3
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• pp.309-313
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• 2007

In Activated Sludge Model, COD fraction of primary settled municipal wastewater was a very important parameter. In this study, the COD fraction was determined using the oxygen utilization rate experiments. Readily biodegradable COD ($S_S$) fraction was observed about 29.7% of influent TCOD. $S_I$, $X_I$, and $X_S$ were analyzed to be 7.6%, 7.3%, and 55.4% of TCOD, respectively. The model used in this study was developed based on ASM3 and modified Bio-P module in order to simulate the existing BNR process. Parameter estimation results showed that $Y_{STO,O2}$, $Y_{STO,NO}$, $Y_{H,O2}$, $Y_{H,NO}$, $Y_{PO4}$, ${\mu}_H$, $b_H$, ${\mu}_A$, $q_{PHA}$, $q_{PP}$ and ${\mu}_{PAO}$ were 0.7, 0.64, 0.61, 0.48, 0.31, 3.9, 0.1, 1.35, 4.98, 1.8 and 0.59, respectively. Using the presented model and the estimated parameters, the simulation of the existing BNR process was successfully conducted.

• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.861-868
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• 2020

The use of polyphenol extracts from radiata pine (Pinus radiata) bark as raw materials for bio-foams was investigated along with the optimal NaOH extraction conditions. The targeted yield of alkaline extracts was 60%, and the targeted pH was 11 to 12. The radiata pine bark was composed of 70% of a 1% NaOH extract, which contained mainly polyphenols, such as proanthocyanidin (PA). As the particle size of the bark decreased, the yield of the 1% NaOH extracts increased from 57 to 87%. A range of NaOH concentrations, liquor ratios, and extraction times were explored to establish an economic polyphenol extraction method. More than 60% of the alkaline extract was extracted, and the pH of the extract was approximately 12 when the optimum extraction conditions were employed, i.e., a liquor to bark ratio of 5:1, a NaOH dosage of 17 to 18% based on the bark weight, and a 1 h extraction time. Following neutralization of the alkaline extract, structural analysis indicated severe structural changes in the PA during the alkaline extraction. Because the alkaline extract was barely soluble in the solvent used for the structural analyses, it is assumed that chemical modification is required to increase the solubility of the alkaline extract for the production of bio-foams.

• Journal of the Korean Magnetic Resonance Society
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• v.18 no.2
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• pp.58-62
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• 2014

Human transmembrane proteins (hTMPs) are closely related to transport, channel formation, signaling, cell to cell interaction, so they are the crucial target of modern medicinal drugs. In order to study the structure and function of these hTMPs, it is important to prepare reasonable amounts of proteins. However, their preparation is seriously difficult and time-consuming due to insufficient yields and low solubility of hTMPs. We tried to produce large amounts of Syndecan-4 transmembrane domain (Syd4-TM) that is related to the healing wounds and tumor for a long time. In this study, we performed the structure determination of Syd4-TM combining the Polarity Index at Slanted Angle (PISA) wheel pattern analysis based on $^{15}N-^1H$ 2D SAMPI-4 solid-state NMR of expressed Syd4-TM and Molecular Dynamics (MD) simulation using Discovery Studio 3.1.